##gff-version 3
##sequence-region BBa_J45299 1 1894
BBa_J45299	.	CDS	22	1757	.	+	0	ID=BBa_J45017;Name=BBa_J45017
BBa_J45299	.	point_mutation	461	461	.	+	0	ID=annotation1897453;Name=g -> a to eliminate PstI site;Parent=BBa_J45017
BBa_J45299	.	CDS	324	1757	.	+	0	ID=annotation1961297;Name=PchA;Parent=BBa_J45017
BBa_J45299	.	stop_codon	325	327	.	+	0	ID=annotation1897456;Name=pchB stop codon;Parent=BBa_J45017
BBa_J45299	.	stop_codon	1752	1757	.	+	0	ID=annotation1897457;Name=pchA double TAA stop codon;Parent=BBa_J45017
BBa_J45299	.	start_codon	324	326	.	+	0	ID=annotation1897454;Name=pchA start codon;Parent=BBa_J45017
BBa_J45299	.	CDS	22	327	.	+	0	ID=annotation1961296;Name=PchB;Parent=BBa_J45017
BBa_J45299	.	start_codon	22	24	.	+	0	ID=annotation1897455;Name=pchB start codon;Parent=BBa_J45017
BBa_J45299	.	terminator	1854	1894	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_J45299	.	stem_loop	1861	1880	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J45299	.	stop_codon	1887	1887	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J45299	.	polyA_site	1881	1894	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J45299	.	engineered_region	1854	1894	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J45299	.	terminator	1766	1845	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_J45299	.	stem_loop	1777	1820	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J45299	.	engineered_region	1766	1845	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J45299	.	ribosome_entry_site	1	15	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_J45299	.	ribosome_entry_site	8	12	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_J45299	.	engineered_region	1	15	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_J45299	.	ribosome_entry_site	1	15	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
>BBa_J45299
attaaagaggagaaatactagatgaaaactcccgaagactgcaccggcctggcggacatccgcgaggccatcgaccgga
tcgacctggatatcgtccaggccctcggccgccgcatggactacgtcaaggcggcgtcgcgcttcaaggccagcgaggc
ggcgattccggcgcccgagcgggtcgccgcgatgctccccgagcgcgcccgctgggccgaggaaaacggactcgacgcg
cccttcgtcgagggactgttcgcgcagatcatccactggtacatcgccgagcagatcaagtactggcgccagacacggg
gtgccgcatgagccggctggcgcccctgagccagtgcctgcacgccttgcgcggcaccttcgagcgcgccatcggccag
gcgcaggcgctcgatcgtccggtgctggtggcggcatcgttcgagatcgacccattggacccgctacaggtattcggtg
cctgggacgaccggcaaacgccctgcctgtactgggaacagcccgagctggcgttcttcgcctggggctgcgccctgga
gctgcaaggccacggcgaacagcgcttcgcccggatcgaggaaaactggcaattgctctgcgccgacgccgtggtcgag
ggcccgctggcgccgcgcctgtgcggcggattccgcttcgatccgcgcggcccgcgcgaggaacactggcaagccttcg
ccgatgccagcctgatgctcgccggcatcaccgtgctgcgcgagggcgaacgctaccgggtactctgccaacacctggc
caagcccggcgaagatgccctggccctggccgcctaccactgctcggcgctactgcgcctgaggcagccggccagacgc
cggccctcggggccgaccgctggcgcgcagggcgacgcttcggcgcaggagcgcaggcaatgggaagccaaggtgagcg
acgcggtaagcagtgtccgccagggacgcttcggcaaggtcgtgctggcccgcacccaggcccggcctctcggcgacat
cgagccgtggcaggtcatcgaacacctgcgtctgcaacatgccgacgcccagctgttcgcctgtcgccgcggcaacgcc
tgcttcctcggcgcctccccggaacgcctggtccgcattcgcgccggcgaggcactcacccatgccctggccgggacca
tcgcccgcggcggcgatgcccaggaagatgcgcggctcggacaggccctgctggacagcgccaaggacaggcacgaaca
ccagttggtggtggaggcgatccgtacggccctggaacccttcagcgaggtgctggaaatccccgatgcgcccggcctg
aaacgactggcgcgagtccagcacctgaacacgccgatccgcgcccgcctcgctgacgcaggcggcatcctgcggctgc
tacaagcgctgcatccgacccccgcggtgggcggctacccacgcagcgcggcgctggactacatccgccagcacgaagg
gatggaccgcggctggtacgccgcgccgctgggctggctcgacggcgaaggcaacggcgatttcctggtggcgctgcgc
tcggccctgctcacgccgggccggggctacctgttcgccggctgcggtctggtaggcgattcggaaccggcccacgagt
atcgcgaaacctgccttaagctcagtgccatgcgggaagctctatccgccataggcggcctggacgaagtgcccttgca
gcgcggcgtcgcctaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttt
tatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata