##gff-version 3
##sequence-region BBa_J45300 1 1957
BBa_J45300	.	promoter	1	54	.	+	0	ID=BBa_R0011;Name=BBa_R0011
BBa_J45300	.	promoter	43	48	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_J45300	.	engineered_region	1	54	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_J45300	.	operator	3	19	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_J45300	.	promoter	20	25	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_J45300	.	operator	26	42	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_J45300	.	ribosome_entry_site	64	78	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_J45300	.	ribosome_entry_site	71	75	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_J45300	.	engineered_region	64	78	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_J45300	.	ribosome_entry_site	64	78	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_J45300	.	terminator	1917	1957	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_J45300	.	stop_codon	1950	1950	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J45300	.	polyA_site	1944	1957	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J45300	.	stem_loop	1924	1943	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J45300	.	engineered_region	1917	1957	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J45300	.	terminator	1829	1908	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_J45300	.	engineered_region	1829	1908	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J45300	.	stem_loop	1840	1883	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J45300	.	CDS	85	1820	.	+	0	ID=BBa_J45017;Name=BBa_J45017
BBa_J45300	.	start_codon	387	389	.	+	0	ID=annotation1897454;Name=pchA start codon;Parent=BBa_J45017
BBa_J45300	.	stop_codon	388	390	.	+	0	ID=annotation1897456;Name=pchB stop codon;Parent=BBa_J45017
BBa_J45300	.	point_mutation	524	524	.	+	0	ID=annotation1897453;Name=g -> a to eliminate PstI site;Parent=BBa_J45017
BBa_J45300	.	CDS	387	1820	.	+	0	ID=annotation1961297;Name=PchA;Parent=BBa_J45017
BBa_J45300	.	stop_codon	1815	1820	.	+	0	ID=annotation1897457;Name=pchA double TAA stop codon;Parent=BBa_J45017
BBa_J45300	.	CDS	85	390	.	+	0	ID=annotation1961296;Name=PchB;Parent=BBa_J45017
BBa_J45300	.	start_codon	85	87	.	+	0	ID=annotation1897455;Name=pchB start codon;Parent=BBa_J45017
>BBa_J45300
aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagattaaagaggagaaat
actagatgaaaactcccgaagactgcaccggcctggcggacatccgcgaggccatcgaccggatcgacctggatatcgt
ccaggccctcggccgccgcatggactacgtcaaggcggcgtcgcgcttcaaggccagcgaggcggcgattccggcgccc
gagcgggtcgccgcgatgctccccgagcgcgcccgctgggccgaggaaaacggactcgacgcgcccttcgtcgagggac
tgttcgcgcagatcatccactggtacatcgccgagcagatcaagtactggcgccagacacggggtgccgcatgagccgg
ctggcgcccctgagccagtgcctgcacgccttgcgcggcaccttcgagcgcgccatcggccaggcgcaggcgctcgatc
gtccggtgctggtggcggcatcgttcgagatcgacccattggacccgctacaggtattcggtgcctgggacgaccggca
aacgccctgcctgtactgggaacagcccgagctggcgttcttcgcctggggctgcgccctggagctgcaaggccacggc
gaacagcgcttcgcccggatcgaggaaaactggcaattgctctgcgccgacgccgtggtcgagggcccgctggcgccgc
gcctgtgcggcggattccgcttcgatccgcgcggcccgcgcgaggaacactggcaagccttcgccgatgccagcctgat
gctcgccggcatcaccgtgctgcgcgagggcgaacgctaccgggtactctgccaacacctggccaagcccggcgaagat
gccctggccctggccgcctaccactgctcggcgctactgcgcctgaggcagccggccagacgccggccctcggggccga
ccgctggcgcgcagggcgacgcttcggcgcaggagcgcaggcaatgggaagccaaggtgagcgacgcggtaagcagtgt
ccgccagggacgcttcggcaaggtcgtgctggcccgcacccaggcccggcctctcggcgacatcgagccgtggcaggtc
atcgaacacctgcgtctgcaacatgccgacgcccagctgttcgcctgtcgccgcggcaacgcctgcttcctcggcgcct
ccccggaacgcctggtccgcattcgcgccggcgaggcactcacccatgccctggccgggaccatcgcccgcggcggcga
tgcccaggaagatgcgcggctcggacaggccctgctggacagcgccaaggacaggcacgaacaccagttggtggtggag
gcgatccgtacggccctggaacccttcagcgaggtgctggaaatccccgatgcgcccggcctgaaacgactggcgcgag
tccagcacctgaacacgccgatccgcgcccgcctcgctgacgcaggcggcatcctgcggctgctacaagcgctgcatcc
gacccccgcggtgggcggctacccacgcagcgcggcgctggactacatccgccagcacgaagggatggaccgcggctgg
tacgccgcgccgctgggctggctcgacggcgaaggcaacggcgatttcctggtggcgctgcgctcggccctgctcacgc
cgggccggggctacctgttcgccggctgcggtctggtaggcgattcggaaccggcccacgagtatcgcgaaacctgcct
taagctcagtgccatgcgggaagctctatccgccataggcggcctggacgaagtgcccttgcagcgcggcgtcgcctaa
taatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcg
gtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata