##gff-version 3
##sequence-region BBa_J45319 1 1892
BBa_J45319	.	CDS	20	1755	.	+	0	ID=BBa_J45017;Name=BBa_J45017
BBa_J45319	.	stop_codon	1750	1755	.	+	0	ID=annotation1897457;Name=pchA double TAA stop codon;Parent=BBa_J45017
BBa_J45319	.	start_codon	322	324	.	+	0	ID=annotation1897454;Name=pchA start codon;Parent=BBa_J45017
BBa_J45319	.	CDS	322	1755	.	+	0	ID=annotation1961297;Name=PchA;Parent=BBa_J45017
BBa_J45319	.	stop_codon	323	325	.	+	0	ID=annotation1897456;Name=pchB stop codon;Parent=BBa_J45017
BBa_J45319	.	CDS	20	325	.	+	0	ID=annotation1961296;Name=PchB;Parent=BBa_J45017
BBa_J45319	.	start_codon	20	22	.	+	0	ID=annotation1897455;Name=pchB start codon;Parent=BBa_J45017
BBa_J45319	.	point_mutation	459	459	.	+	0	ID=annotation1897453;Name=g -> a to eliminate PstI site;Parent=BBa_J45017
BBa_J45319	.	terminator	1852	1892	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_J45319	.	engineered_region	1852	1892	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J45319	.	polyA_site	1879	1892	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J45319	.	stop_codon	1885	1885	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J45319	.	stem_loop	1859	1878	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J45319	.	ribosome_entry_site	1	13	.	+	0	ID=BBa_B0032;Name=BBa_B0032
BBa_J45319	.	ribosome_entry_site	1	13	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_J45319	.	engineered_region	1	13	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_J45319	.	ribosome_entry_site	7	10	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_J45319	.	terminator	1764	1843	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_J45319	.	engineered_region	1764	1843	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J45319	.	stem_loop	1775	1818	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
>BBa_J45319
tcacacaggaaagtactagatgaaaactcccgaagactgcaccggcctggcggacatccgcgaggccatcgaccggatc
gacctggatatcgtccaggccctcggccgccgcatggactacgtcaaggcggcgtcgcgcttcaaggccagcgaggcgg
cgattccggcgcccgagcgggtcgccgcgatgctccccgagcgcgcccgctgggccgaggaaaacggactcgacgcgcc
cttcgtcgagggactgttcgcgcagatcatccactggtacatcgccgagcagatcaagtactggcgccagacacggggt
gccgcatgagccggctggcgcccctgagccagtgcctgcacgccttgcgcggcaccttcgagcgcgccatcggccaggc
gcaggcgctcgatcgtccggtgctggtggcggcatcgttcgagatcgacccattggacccgctacaggtattcggtgcc
tgggacgaccggcaaacgccctgcctgtactgggaacagcccgagctggcgttcttcgcctggggctgcgccctggagc
tgcaaggccacggcgaacagcgcttcgcccggatcgaggaaaactggcaattgctctgcgccgacgccgtggtcgaggg
cccgctggcgccgcgcctgtgcggcggattccgcttcgatccgcgcggcccgcgcgaggaacactggcaagccttcgcc
gatgccagcctgatgctcgccggcatcaccgtgctgcgcgagggcgaacgctaccgggtactctgccaacacctggcca
agcccggcgaagatgccctggccctggccgcctaccactgctcggcgctactgcgcctgaggcagccggccagacgccg
gccctcggggccgaccgctggcgcgcagggcgacgcttcggcgcaggagcgcaggcaatgggaagccaaggtgagcgac
gcggtaagcagtgtccgccagggacgcttcggcaaggtcgtgctggcccgcacccaggcccggcctctcggcgacatcg
agccgtggcaggtcatcgaacacctgcgtctgcaacatgccgacgcccagctgttcgcctgtcgccgcggcaacgcctg
cttcctcggcgcctccccggaacgcctggtccgcattcgcgccggcgaggcactcacccatgccctggccgggaccatc
gcccgcggcggcgatgcccaggaagatgcgcggctcggacaggccctgctggacagcgccaaggacaggcacgaacacc
agttggtggtggaggcgatccgtacggccctggaacccttcagcgaggtgctggaaatccccgatgcgcccggcctgaa
acgactggcgcgagtccagcacctgaacacgccgatccgcgcccgcctcgctgacgcaggcggcatcctgcggctgcta
caagcgctgcatccgacccccgcggtgggcggctacccacgcagcgcggcgctggactacatccgccagcacgaaggga
tggaccgcggctggtacgccgcgccgctgggctggctcgacggcgaaggcaacggcgatttcctggtggcgctgcgctc
ggccctgctcacgccgggccggggctacctgttcgccggctgcggtctggtaggcgattcggaaccggcccacgagtat
cgcgaaacctgccttaagctcagtgccatgcgggaagctctatccgccataggcggcctggacgaagtgcccttgcagc
gcggcgtcgcctaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgtttta
tctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata