##gff-version 3
##sequence-region BBa_J45320 1 1955
BBa_J45320	.	terminator	1915	1955	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_J45320	.	engineered_region	1915	1955	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J45320	.	stem_loop	1922	1941	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J45320	.	polyA_site	1942	1955	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J45320	.	stop_codon	1948	1948	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J45320	.	promoter	1	54	.	+	0	ID=BBa_R0011;Name=BBa_R0011
BBa_J45320	.	engineered_region	1	54	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_J45320	.	operator	26	42	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_J45320	.	promoter	43	48	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_J45320	.	operator	3	19	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_J45320	.	promoter	20	25	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_J45320	.	terminator	1827	1906	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_J45320	.	engineered_region	1827	1906	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J45320	.	stem_loop	1838	1881	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J45320	.	ribosome_entry_site	64	76	.	+	0	ID=BBa_B0032;Name=BBa_B0032
BBa_J45320	.	ribosome_entry_site	64	76	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_J45320	.	engineered_region	64	76	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_J45320	.	ribosome_entry_site	70	73	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_J45320	.	CDS	83	1818	.	+	0	ID=BBa_J45017;Name=BBa_J45017
BBa_J45320	.	start_codon	83	85	.	+	0	ID=annotation1897455;Name=pchB start codon;Parent=BBa_J45017
BBa_J45320	.	stop_codon	386	388	.	+	0	ID=annotation1897456;Name=pchB stop codon;Parent=BBa_J45017
BBa_J45320	.	point_mutation	522	522	.	+	0	ID=annotation1897453;Name=g -> a to eliminate PstI site;Parent=BBa_J45017
BBa_J45320	.	stop_codon	1813	1818	.	+	0	ID=annotation1897457;Name=pchA double TAA stop codon;Parent=BBa_J45017
BBa_J45320	.	start_codon	385	387	.	+	0	ID=annotation1897454;Name=pchA start codon;Parent=BBa_J45017
BBa_J45320	.	CDS	83	388	.	+	0	ID=annotation1961296;Name=PchB;Parent=BBa_J45017
BBa_J45320	.	CDS	385	1818	.	+	0	ID=annotation1961297;Name=PchA;Parent=BBa_J45017
>BBa_J45320
aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagtcacacaggaaagtac
tagatgaaaactcccgaagactgcaccggcctggcggacatccgcgaggccatcgaccggatcgacctggatatcgtcc
aggccctcggccgccgcatggactacgtcaaggcggcgtcgcgcttcaaggccagcgaggcggcgattccggcgcccga
gcgggtcgccgcgatgctccccgagcgcgcccgctgggccgaggaaaacggactcgacgcgcccttcgtcgagggactg
ttcgcgcagatcatccactggtacatcgccgagcagatcaagtactggcgccagacacggggtgccgcatgagccggct
ggcgcccctgagccagtgcctgcacgccttgcgcggcaccttcgagcgcgccatcggccaggcgcaggcgctcgatcgt
ccggtgctggtggcggcatcgttcgagatcgacccattggacccgctacaggtattcggtgcctgggacgaccggcaaa
cgccctgcctgtactgggaacagcccgagctggcgttcttcgcctggggctgcgccctggagctgcaaggccacggcga
acagcgcttcgcccggatcgaggaaaactggcaattgctctgcgccgacgccgtggtcgagggcccgctggcgccgcgc
ctgtgcggcggattccgcttcgatccgcgcggcccgcgcgaggaacactggcaagccttcgccgatgccagcctgatgc
tcgccggcatcaccgtgctgcgcgagggcgaacgctaccgggtactctgccaacacctggccaagcccggcgaagatgc
cctggccctggccgcctaccactgctcggcgctactgcgcctgaggcagccggccagacgccggccctcggggccgacc
gctggcgcgcagggcgacgcttcggcgcaggagcgcaggcaatgggaagccaaggtgagcgacgcggtaagcagtgtcc
gccagggacgcttcggcaaggtcgtgctggcccgcacccaggcccggcctctcggcgacatcgagccgtggcaggtcat
cgaacacctgcgtctgcaacatgccgacgcccagctgttcgcctgtcgccgcggcaacgcctgcttcctcggcgcctcc
ccggaacgcctggtccgcattcgcgccggcgaggcactcacccatgccctggccgggaccatcgcccgcggcggcgatg
cccaggaagatgcgcggctcggacaggccctgctggacagcgccaaggacaggcacgaacaccagttggtggtggaggc
gatccgtacggccctggaacccttcagcgaggtgctggaaatccccgatgcgcccggcctgaaacgactggcgcgagtc
cagcacctgaacacgccgatccgcgcccgcctcgctgacgcaggcggcatcctgcggctgctacaagcgctgcatccga
cccccgcggtgggcggctacccacgcagcgcggcgctggactacatccgccagcacgaagggatggaccgcggctggta
cgccgcgccgctgggctggctcgacggcgaaggcaacggcgatttcctggtggcgctgcgctcggccctgctcacgccg
ggccggggctacctgttcgccggctgcggtctggtaggcgattcggaaccggcccacgagtatcgcgaaacctgcctta
agctcagtgccatgcgggaagctctatccgccataggcggcctggacgaagtgcccttgcagcgcggcgtcgcctaata
atactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggt
gaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata