##gff-version 3
##sequence-region BBa_J45397 1 1178
BBa_J45397	.	ribosome_entry_site	1	15	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_J45397	.	engineered_region	1	15	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_J45397	.	ribosome_entry_site	8	12	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_J45397	.	ribosome_entry_site	1	15	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_J45397	.	ribosome_entry_site	1164	1178	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_J45397	.	engineered_region	1164	1178	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_J45397	.	ribosome_entry_site	1171	1175	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_J45397	.	ribosome_entry_site	1164	1178	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_J45397	.	CDS	22	1155	.	+	0	ID=BBa_J45008;Name=BBa_J45008
BBa_J45397	.	point_mutation	792	792	.	+	0	ID=annotation1894553;Name=a->g to eliminate SpeI;Parent=BBa_J45008
BBa_J45397	.	stop_codon	1150	1155	.	+	0	ID=annotation1883625;Name=double TAA stop codon;Parent=BBa_J45008
BBa_J45397	.	start_codon	22	24	.	+	0	ID=annotation1883624;Name=start codon;Parent=BBa_J45008
BBa_J45397	.	CDS	22	1155	.	+	0	ID=annotation1961299;Name=BAT2;Parent=BBa_J45008
>BBa_J45397
attaaagaggagaaatactagatgaccttggcacccctagacgcctccaaagttaagataactaccacacaacatgcat
ctaagccaaaaccgaacagtgagttagtgtttggcaagagcttcacggaccacatgttaactgcggaatggacagctga
aaaagggtggggtaccccagagattaaaccttatcaaaatctgtctttagacccttccgcggtggttttccattatgct
tttgagctattcgaagggatgaaggcttacagaacggtggacaacaaaattacaatgtttcgtccagatatgaatatga
agcgcatgaataagtctgctcagagaatctgtttgccaacgttcgacccagaagagttgattaccctaattgggaaact
gatccagcaagataagtgcttagttcctgaaggaaaaggttactctttatatatcaggcctacattaatcggcactacg
gccggtttaggggtttccacgcctgatagagccttgctatatgtcatttgctgccctgtgggtccttattacaaaactg
gatttaaggcggtcagactggaagccactgattatgccacaagagcttggccaggaggctgtggtgacaagaaactagg
tgcaaactacgccccctgcgtcctgccacaattgcaagctgcttcaaggggttaccaacaaaatttatggctatttggt
ccaaataacaacattactgaagtcggcaccatgaatgcttttttcgtgtttaaagatagtaaaacgggcaagaaggaac
tggttactgctccactagacggtaccattttggaaggtgttactagggattccattttaaatcttgctaaagaaagact
cgaaccaagtgaatggaccattagtgaacgctacttcactataggcgaagttactgagagatccaagaacggtgaacta
cttgaagcctttggttctggtactgctgcgattgtttctcccattaaggaaatcggctggaaaggcgaacaaattaata
ttccgttgttgcccggcgaacaaaccggtccattggccaaagaagttgcacaatggattaatggaatccaatatggcga
gactgagcatggcaattggtcaagggttgttactgatttgaactaataatactagagattaaagaggagaaa