LOCUS BBa_J45398 3014 bp DNA linear 22-Dec-2024 DEFINITION BAT2 & THI3 enzyme generator ACCESSION BBa_J45398 VERSION BBa_J45398.1 FEATURES Location/Qualifiers RBS 1..15 /label=RBS-1\Strong RBS 8..12 /label=RBS misc_feature 1..15 /label=BBa_B0030 RBS 1..15 /label=BBa_B0030 misc_feature 22..24 /label=start codon misc_feature 1150..1155 /label=double TAA stop codon misc_feature 792..792 /label=a->g to eliminate SpeI CDS 22..1155 /label=BAT2 CDS 22..1155 /label=BBa_J45008 RBS 1164..1178 /label=RBS-1\Strong RBS 1171..1175 /label=RBS misc_feature 1164..1178 /label=BBa_B0030 RBS 1164..1178 /label=BBa_B0030 misc_feature 1190..1190 /label=t->c to eliminate EcoRI, to do misc_feature 2090..2090 /label=a->g to eliminate EcoRI, to do misc_feature 2789..2789 /label=a->g to eliminate EcoRI, to do misc_feature 2975..2975 /label=a->g to eliminate EcoRI, to do misc_feature 1883..1883 /label=t->c to eliminate SpeI, to do CDS 1185..3014 /label=THI3 misc_feature 1185..1187 /label=start codon misc_feature 3012..3014 /label=stop codon CDS 1185..3014 /label=BBa_J45009 ORIGIN 1 attaaagagg agaaatacta gatgaccttg gcacccctag acgcctccaa agttaagata 61 actaccacac aacatgcatc taagccaaaa ccgaacagtg agttagtgtt tggcaagagc 121 ttcacggacc acatgttaac tgcggaatgg acagctgaaa aagggtgggg taccccagag 181 attaaacctt atcaaaatct gtctttagac ccttccgcgg tggttttcca ttatgctttt 241 gagctattcg aagggatgaa ggcttacaga acggtggaca acaaaattac aatgtttcgt 301 ccagatatga atatgaagcg catgaataag tctgctcaga gaatctgttt gccaacgttc 361 gacccagaag agttgattac cctaattggg aaactgatcc agcaagataa gtgcttagtt 421 cctgaaggaa aaggttactc tttatatatc aggcctacat taatcggcac tacggccggt 481 ttaggggttt ccacgcctga tagagccttg ctatatgtca tttgctgccc tgtgggtcct 541 tattacaaaa ctggatttaa ggcggtcaga ctggaagcca ctgattatgc cacaagagct 601 tggccaggag gctgtggtga caagaaacta ggtgcaaact acgccccctg cgtcctgcca 661 caattgcaag ctgcttcaag gggttaccaa caaaatttat ggctatttgg tccaaataac 721 aacattactg aagtcggcac catgaatgct tttttcgtgt ttaaagatag taaaacgggc 781 aagaaggaac tggttactgc tccactagac ggtaccattt tggaaggtgt tactagggat 841 tccattttaa atcttgctaa agaaagactc gaaccaagtg aatggaccat tagtgaacgc 901 tacttcacta taggcgaagt tactgagaga tccaagaacg gtgaactact tgaagccttt 961 ggttctggta ctgctgcgat tgtttctccc attaaggaaa tcggctggaa aggcgaacaa 1021 attaatattc cgttgttgcc cggcgaacaa accggtccat tggccaaaga agttgcacaa 1081 tggattaatg gaatccaata tggcgagact gagcatggca attggtcaag ggttgttact 1141 gatttgaact aataatacta gagattaaag aggagaaata ctagatgaat tctagctata 1201 cacagagata tgcactgccg aagtgtatag caatatcaga ttatcttttc catcggctca 1261 accagctgaa catacatacc atatttggac tctccggaga atttagcatg ccgttgctgg 1321 ataaactata caacattccg aacttacgat gggccggtaa ttctaatgag ttaaatgctg 1381 cctacgcagc agatggatac tcacgactaa aaggcttggg atgtctcata acaacctttg 1441 gtgtaggcga attatcggca atcaatggcg tggccggatc ttacgctgaa catgtaggaa 1501 tacttcacat agtgggtatg ccgccaacaa gtgcacaaac gaaacaacta ctactgcatc 1561 atactctggg caatggtgat ttcacggtat ttcatagaat agccagtgat gtagcatgct 1621 atacaacatt gattattgac tctgaattat gtgccgacga agtcgataag tgcatcaaaa 1681 aggcttggat agaacagagg ccagtataca tgggcatgcc tgtcaaccag gtaaatctcc 1741 cgattgaatc agcaaggctt aatacacctc tggatttaca attgcataaa aacgacccag 1801 acgtagagaa agaagttatt tctcgaatat tgagttttat atacaaaagc cagaatccgg 1861 caatcatcgt agatgcatgt actagtcgac agaatttaat cgaggagact aaagagcttt 1921 gtaataggct taaatttcca gtttttgtta cacctatggg taagggtaca gtaaacgaaa 1981 cagacccgca atttgggggc gtattcacgg gctcgatatc agccccagaa gtaagagaag 2041 tagttgattt tgccgatttt atcatcgtca ttggttgcat gctctccgaa ttcagcacgt 2101 caactttcca cttccaatat aaaactaaga attgtgcgct actatattct acatctgtga 2161 aattgaaaaa tgccacatat cctgacttga gcattaaatt actactacag aaaatattag 2221 caaatcttga tgaatctaaa ctgtcttacc aaccaagcga acaacccagt atgatggttc 2281 caagacctta cccagcagga aatgtcctct tgagacaaga atgggtctgg aatgaaatat 2341 cccattggtt ccaaccaggt gacataatca taacagaaac tggtgcttct gcatttggag 2401 ttaaccagac cagatttccg gtaaatacac taggtatttc gcaagctctt tggggatctg 2461 tcggatatac aatgggggcg tgtcttgggg cagaatttgc tgttcaagag ataaacaagg 2521 ataaattccc cgcaactaaa catagagtta ttctgtttat gggtgacggt gctttccaat 2581 tgacagttca agaattatcc acaattgtta agtggggatt gacaccttat atttttgtga 2641 tgaataacca aggttactct gtggacaggt ttttgcatca caggtcagat gctagttatt 2701 acgatatcca accttggaac tacttgggat tattgcgagt atttggttgc acgaactacg 2761 aaacgaaaaa aattattact gttggagaat tcagatccat gatcagtgac ccaaactttg 2821 cgaccaatga caaaattcgg atgatagaga ttatgctacc accaagggat gttccacagg 2881 ctctgcttga caggtgggtg gtagaaaaag aacagagcaa acaagtgcaa gaggagaacg 2941 aaaattctag cgcagtaaat acgccaactc cagaattcca accacttcta aaaaaaaatc 3001 aagttggata ctga //