##gff-version 3
##sequence-region BBa_J45399 1 3151
BBa_J45399	.	CDS	22	1155	.	+	0	ID=BBa_J45008;Name=BBa_J45008
BBa_J45399	.	CDS	22	1155	.	+	0	ID=annotation1961299;Name=BAT2;Parent=BBa_J45008
BBa_J45399	.	start_codon	22	24	.	+	0	ID=annotation1883624;Name=start codon;Parent=BBa_J45008
BBa_J45399	.	point_mutation	792	792	.	+	0	ID=annotation1894553;Name=a->g to eliminate SpeI;Parent=BBa_J45008
BBa_J45399	.	stop_codon	1150	1155	.	+	0	ID=annotation1883625;Name=double TAA stop codon;Parent=BBa_J45008
BBa_J45399	.	ribosome_entry_site	1164	1178	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_J45399	.	engineered_region	1164	1178	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_J45399	.	ribosome_entry_site	1164	1178	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_J45399	.	ribosome_entry_site	1171	1175	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_J45399	.	ribosome_entry_site	1	15	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_J45399	.	engineered_region	1	15	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_J45399	.	ribosome_entry_site	1	15	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_J45399	.	ribosome_entry_site	8	12	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_J45399	.	terminator	3023	3102	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_J45399	.	stem_loop	3034	3077	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J45399	.	engineered_region	3023	3102	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J45399	.	terminator	3111	3151	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_J45399	.	engineered_region	3111	3151	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J45399	.	stop_codon	3144	3144	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J45399	.	polyA_site	3138	3151	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J45399	.	stem_loop	3118	3137	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J45399	.	CDS	1185	3014	.	+	0	ID=BBa_J45009;Name=BBa_J45009
BBa_J45399	.	point_mutation	2789	2789	.	+	0	ID=annotation1891630;Name=a->g to eliminate EcoRI, to do;Parent=BBa_J45009
BBa_J45399	.	point_mutation	1190	1190	.	+	0	ID=annotation1891628;Name=t->c to eliminate EcoRI, to do;Parent=BBa_J45009
BBa_J45399	.	point_mutation	2975	2975	.	+	0	ID=annotation1891631;Name=a->g to eliminate EcoRI, to do;Parent=BBa_J45009
BBa_J45399	.	stop_codon	3012	3014	.	+	0	ID=annotation1961304;Name=stop codon;Parent=BBa_J45009
BBa_J45399	.	start_codon	1185	1187	.	+	0	ID=annotation1961303;Name=start codon;Parent=BBa_J45009
BBa_J45399	.	point_mutation	1883	1883	.	+	0	ID=annotation1891632;Name=t->c to eliminate SpeI, to do;Parent=BBa_J45009
BBa_J45399	.	CDS	1185	3014	.	+	0	ID=annotation1961298;Name=THI3;Parent=BBa_J45009
BBa_J45399	.	point_mutation	2090	2090	.	+	0	ID=annotation1891629;Name=a->g to eliminate EcoRI, to do;Parent=BBa_J45009
>BBa_J45399
attaaagaggagaaatactagatgaccttggcacccctagacgcctccaaagttaagataactaccacacaacatgcat
ctaagccaaaaccgaacagtgagttagtgtttggcaagagcttcacggaccacatgttaactgcggaatggacagctga
aaaagggtggggtaccccagagattaaaccttatcaaaatctgtctttagacccttccgcggtggttttccattatgct
tttgagctattcgaagggatgaaggcttacagaacggtggacaacaaaattacaatgtttcgtccagatatgaatatga
agcgcatgaataagtctgctcagagaatctgtttgccaacgttcgacccagaagagttgattaccctaattgggaaact
gatccagcaagataagtgcttagttcctgaaggaaaaggttactctttatatatcaggcctacattaatcggcactacg
gccggtttaggggtttccacgcctgatagagccttgctatatgtcatttgctgccctgtgggtccttattacaaaactg
gatttaaggcggtcagactggaagccactgattatgccacaagagcttggccaggaggctgtggtgacaagaaactagg
tgcaaactacgccccctgcgtcctgccacaattgcaagctgcttcaaggggttaccaacaaaatttatggctatttggt
ccaaataacaacattactgaagtcggcaccatgaatgcttttttcgtgtttaaagatagtaaaacgggcaagaaggaac
tggttactgctccactagacggtaccattttggaaggtgttactagggattccattttaaatcttgctaaagaaagact
cgaaccaagtgaatggaccattagtgaacgctacttcactataggcgaagttactgagagatccaagaacggtgaacta
cttgaagcctttggttctggtactgctgcgattgtttctcccattaaggaaatcggctggaaaggcgaacaaattaata
ttccgttgttgcccggcgaacaaaccggtccattggccaaagaagttgcacaatggattaatggaatccaatatggcga
gactgagcatggcaattggtcaagggttgttactgatttgaactaataatactagagattaaagaggagaaatactaga
tgaattctagctatacacagagatatgcactgccgaagtgtatagcaatatcagattatcttttccatcggctcaacca
gctgaacatacataccatatttggactctccggagaatttagcatgccgttgctggataaactatacaacattccgaac
ttacgatgggccggtaattctaatgagttaaatgctgcctacgcagcagatggatactcacgactaaaaggcttgggat
gtctcataacaacctttggtgtaggcgaattatcggcaatcaatggcgtggccggatcttacgctgaacatgtaggaat
acttcacatagtgggtatgccgccaacaagtgcacaaacgaaacaactactactgcatcatactctgggcaatggtgat
ttcacggtatttcatagaatagccagtgatgtagcatgctatacaacattgattattgactctgaattatgtgccgacg
aagtcgataagtgcatcaaaaaggcttggatagaacagaggccagtatacatgggcatgcctgtcaaccaggtaaatct
cccgattgaatcagcaaggcttaatacacctctggatttacaattgcataaaaacgacccagacgtagagaaagaagtt
atttctcgaatattgagttttatatacaaaagccagaatccggcaatcatcgtagatgcatgtactagtcgacagaatt
taatcgaggagactaaagagctttgtaataggcttaaatttccagtttttgttacacctatgggtaagggtacagtaaa
cgaaacagacccgcaatttgggggcgtattcacgggctcgatatcagccccagaagtaagagaagtagttgattttgcc
gattttatcatcgtcattggttgcatgctctccgaattcagcacgtcaactttccacttccaatataaaactaagaatt
gtgcgctactatattctacatctgtgaaattgaaaaatgccacatatcctgacttgagcattaaattactactacagaa
aatattagcaaatcttgatgaatctaaactgtcttaccaaccaagcgaacaacccagtatgatggttccaagaccttac
ccagcaggaaatgtcctcttgagacaagaatgggtctggaatgaaatatcccattggttccaaccaggtgacataatca
taacagaaactggtgcttctgcatttggagttaaccagaccagatttccggtaaatacactaggtatttcgcaagctct
ttggggatctgtcggatatacaatgggggcgtgtcttggggcagaatttgctgttcaagagataaacaaggataaattc
cccgcaactaaacatagagttattctgtttatgggtgacggtgctttccaattgacagttcaagaattatccacaattg
ttaagtggggattgacaccttatatttttgtgatgaataaccaaggttactctgtggacaggtttttgcatcacaggtc
agatgctagttattacgatatccaaccttggaactacttgggattattgcgagtatttggttgcacgaactacgaaacg
aaaaaaattattactgttggagaattcagatccatgatcagtgacccaaactttgcgaccaatgacaaaattcggatga
tagagattatgctaccaccaagggatgttccacaggctctgcttgacaggtgggtggtagaaaaagaacagagcaaaca
agtgcaagaggagaacgaaaattctagcgcagtaaatacgccaactccagaattccaaccacttctaaaaaaaaatcaa
gttggatactgatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgt
tgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata