BBa_J45399
type: ComponentDefinition
persistent identity: BBa J45399
display id: BBa_J45399
version: 1
was generated by: iGEM to SBOL conversion
description: RBS + BAT2 + RBS + THI3 + Term
created: 2006-10-27T11:00:00Z
modified: 2015-08-31T04:08:49Z
mutable provenance: coming
owned by: james
owned by: myers
mutable description: This part is an intermediate to the protein generator BBa_45400.
discontinued: true
dominant: false
experience: experience/None
group u list: _84_
m user id: 0
owner id: 977
owning group id: 84
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable notes: coming
star: false
creator: Andr?? Green II
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_J45008
component: BBa_B0030
component: BBa_J45009
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0030
sequence annotation: BBa_J45008
sequence annotation: BBa_J45009
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_J45399_sequence/1

BBa_J45008
type: Component
persistent identity: component1961104
display id: component1961104
version: 1
top level: BBa_J45399
definition: BAT2
access: public

BBa_B0030
type: Component
persistent identity: component1961099
display id: component1961099
version: 1
top level: BBa_J45399
definition: BBa_B0030
access: public

BBa_J45009
type: Component
persistent identity: component1961113
display id: component1961113
version: 1
top level: BBa_J45399
definition: THI3
access: public

BBa_B0030
type: Component
persistent identity: component1961106
display id: component1961106
version: 1
top level: BBa_J45399
definition: BBa_B0030
access: public

BBa_B0012
type: Component
persistent identity: component1961116
display id: component1961116
version: 1
top level: BBa_J45399
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1961114
display id: component1961114
version: 1
top level: BBa_J45399
definition: BBa_B0010
access: public

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1961099
display id: annotation1961099
version: 1
top level: BBa_J45399
location: https://synbiohub.org/public/igem/BBa_J45399/annotation1961099/range1961099/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_J45399/annotation1961099/range1961099/1
type: Range
persistent identity: range1961099
display id: range1961099
version: 1
top level: BBa_J45399
direction: direction/forward
start: 1
end: 15
orientation: inline

BBa_J45008
type: SequenceAnnotation
persistent identity: annotation1961104
display id: annotation1961104
version: 1
top level: BBa_J45399
location: https://synbiohub.org/public/igem/BBa_J45399/annotation1961104/range1961104/1
component: BBa_J45008

https://synbiohub.org/public/igem/BBa_J45399/annotation1961104/range1961104/1
type: Range
persistent identity: range1961104
display id: range1961104
version: 1
top level: BBa_J45399
direction: direction/reverse
start: 22
end: 1155
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1961106
display id: annotation1961106
version: 1
top level: BBa_J45399
location: https://synbiohub.org/public/igem/BBa_J45399/annotation1961106/range1961106/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_J45399/annotation1961106/range1961106/1
type: Range
persistent identity: range1961106
display id: range1961106
version: 1
top level: BBa_J45399
direction: direction/forward
start: 1164
end: 1178
orientation: inline

BBa_J45009
type: SequenceAnnotation
persistent identity: annotation1961113
display id: annotation1961113
version: 1
top level: BBa_J45399
location: https://synbiohub.org/public/igem/BBa_J45399/annotation1961113/range1961113/1
component: BBa_J45009

https://synbiohub.org/public/igem/BBa_J45399/annotation1961113/range1961113/1
type: Range
persistent identity: range1961113
display id: range1961113
version: 1
top level: BBa_J45399
direction: direction/reverse
start: 1185
end: 3014
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1961114
display id: annotation1961114
version: 1
top level: BBa_J45399
location: https://synbiohub.org/public/igem/BBa_J45399/annotation1961114/range1961114/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J45399/annotation1961114/range1961114/1
type: Range
persistent identity: range1961114
display id: range1961114
version: 1
top level: BBa_J45399
direction: direction/forward
start: 3023
end: 3102
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1961116
display id: annotation1961116
version: 1
top level: BBa_J45399
location: https://synbiohub.org/public/igem/BBa_J45399/annotation1961116/range1961116/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J45399/annotation1961116/range1961116/1
type: Range
persistent identity: range1961116
display id: range1961116
version: 1
top level: BBa_J45399
direction: direction/forward
start: 3111
end: 3151
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

BBa_B0030
type: ComponentDefinition
persistent identity: BBa B0030
display id: BBa_B0030
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.1 (strong) -- modified from R. Weiss
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _44_46_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: RBS-1\Strong
sequence annotation: BBa_B0030
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0030_sequence/1

RBS-1\Strong
type: SequenceAnnotation
persistent identity: annotation1701
display id: annotation1701
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
type: Range
persistent identity: range1701
display id: range1701
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation7025
display id: annotation7025
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
type: Range
persistent identity: range7025
display id: range7025
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1702
display id: annotation1702
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
type: Range
persistent identity: range1702
display id: range1702
version: 1
top level: BBa_B0030
direction: direction/forward
start: 8
end: 12
orientation: inline

BAT2
type: ComponentDefinition
persistent identity: BBa J45008
display id: BBa_J45008
version: 1
was generated by: iGEM to SBOL conversion
description: branched-chain amino acid transaminase (BAT2); converts leucine to alpha-ketoisocaproate
created: 2006-06-06T11:00:00Z
modified: 2015-08-31T04:08:49Z
mutable provenance: dkfkas
owned by: james
owned by: myers
mutable description: fd;lsfas
discontinued: false
dominant: false
experience: experience/Fails
group u list: _84_
m user id: 0
owner id: 642
owning group id: 84
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: dakfdas;
star: false
creator: Veena Venkatachalam
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: BAT2
sequence annotation: start codon
sequence annotation: a->g to eliminate SpeI
sequence annotation: double TAA stop codon
sequence: https://synbiohub.org/public/igem/BBa_J45008_sequence/1

BAT2
type: SequenceAnnotation
persistent identity: annotation1961299
display id: annotation1961299
version: 1
top level: BAT2
location: https://synbiohub.org/public/igem/BBa_J45008/annotation1961299/range1961299/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_J45008/annotation1961299/range1961299/1
type: Range
persistent identity: range1961299
display id: range1961299
version: 1
top level: BAT2
direction: direction/forward
start: 1
end: 1134
orientation: inline

start codon
type: SequenceAnnotation
persistent identity: annotation1883624
display id: annotation1883624
version: 1
top level: BAT2
location: https://synbiohub.org/public/igem/BBa_J45008/annotation1883624/range1883624/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_J45008/annotation1883624/range1883624/1
type: Range
persistent identity: range1883624
display id: range1883624
version: 1
top level: BAT2
direction: direction/forward
start: 1
end: 3
orientation: inline

a->g to eliminate SpeI
type: SequenceAnnotation
persistent identity: annotation1894553
display id: annotation1894553
version: 1
top level: BAT2
location: https://synbiohub.org/public/igem/BBa_J45008/annotation1894553/range1894553/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_J45008/annotation1894553/range1894553/1
type: Range
persistent identity: range1894553
display id: range1894553
version: 1
top level: BAT2
direction: direction/forward
start: 771
end: 771
orientation: inline

double TAA stop codon
type: SequenceAnnotation
persistent identity: annotation1883625
display id: annotation1883625
version: 1
top level: BAT2
location: https://synbiohub.org/public/igem/BBa_J45008/annotation1883625/range1883625/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_J45008/annotation1883625/range1883625/1
type: Range
persistent identity: range1883625
display id: range1883625
version: 1
top level: BAT2
direction: direction/forward
start: 1129
end: 1134
orientation: inline

THI3
type: ComponentDefinition
persistent identity: BBa J45009
display id: BBa_J45009
version: 1
was generated by: iGEM to SBOL conversion
description: ketoisocaproate decarboxylase (THI3); converts alpha-ketoisocaproate to 3-methylbutanal
created: 2006-06-06T11:00:00Z
modified: 2015-08-31T04:08:49Z
mutable provenance: dfd
owned by: james
owned by: myers
mutable description: dfsd
discontinued: false
dominant: false
experience: experience/Fails
group u list: _84_
m user id: 0
owner id: 642
owning group id: 84
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable notes: dfd
star: false
creator: Veena Venkatachalam
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: start codon
sequence annotation: THI3
sequence annotation: t->c to eliminate EcoRI, to do
sequence annotation: t->c to eliminate SpeI, to do
sequence annotation: a->g to eliminate EcoRI, to do
sequence annotation: stop codon
sequence: https://synbiohub.org/public/igem/BBa_J45009_sequence/1

start codon
type: SequenceAnnotation
persistent identity: annotation1961303
display id: annotation1961303
version: 1
top level: THI3
location: https://synbiohub.org/public/igem/BBa_J45009/annotation1961303/range1961303/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_J45009/annotation1961303/range1961303/1
type: Range
persistent identity: range1961303
display id: range1961303
version: 1
top level: THI3
direction: direction/forward
start: 1
end: 3
orientation: inline

THI3
type: SequenceAnnotation
persistent identity: annotation1961298
display id: annotation1961298
version: 1
top level: THI3
location: https://synbiohub.org/public/igem/BBa_J45009/annotation1961298/range1961298/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_J45009/annotation1961298/range1961298/1
type: Range
persistent identity: range1961298
display id: range1961298
version: 1
top level: THI3
direction: direction/forward
start: 1
end: 1830
orientation: inline

t->c to eliminate EcoRI, to do
type: SequenceAnnotation
persistent identity: annotation1891628
display id: annotation1891628
version: 1
top level: THI3
location: https://synbiohub.org/public/igem/BBa_J45009/annotation1891628/range1891628/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_J45009/annotation1891628/range1891628/1
type: Range
persistent identity: range1891628
display id: range1891628
version: 1
top level: THI3
direction: direction/forward
start: 6
end: 6
orientation: inline

t->c to eliminate SpeI, to do
type: SequenceAnnotation
persistent identity: annotation1891632
display id: annotation1891632
version: 1
top level: THI3
location: https://synbiohub.org/public/igem/BBa_J45009/annotation1891632/range1891632/1
role: SO:1000008
role: feature/s mutation

https://synbiohub.org/public/igem/BBa_J45009/annotation1891632/range1891632/1
type: Range
persistent identity: range1891632
display id: range1891632
version: 1
top level: THI3
direction: direction/forward
start: 699
end: 699
orientation: inline

a->g to eliminate EcoRI, to do
type: SequenceAnnotation
persistent identity: annotation1891629
display id: annotation1891629
version: 1
top level: THI3
location: https://synbiohub.org/public/igem/BBa_J45009/annotation1891629/range1891629/1
role: SO:1000008
role: feature/s mutation

https://synbiohub.org/public/igem/BBa_J45009/annotation1891629/range1891629/1
type: Range
persistent identity: range1891629
display id: range1891629
version: 1
top level: THI3
direction: direction/forward
start: 906
end: 906
orientation: inline

a->g to eliminate EcoRI, to do
type: SequenceAnnotation
persistent identity: annotation1891630
display id: annotation1891630
version: 1
top level: THI3
location: https://synbiohub.org/public/igem/BBa_J45009/annotation1891630/range1891630/1
role: SO:1000008
role: feature/s mutation

https://synbiohub.org/public/igem/BBa_J45009/annotation1891630/range1891630/1
type: Range
persistent identity: range1891630
display id: range1891630
version: 1
top level: THI3
direction: direction/forward
start: 1605
end: 1605
orientation: inline

a->g to eliminate EcoRI, to do
type: SequenceAnnotation
persistent identity: annotation1891631
display id: annotation1891631
version: 1
top level: THI3
location: https://synbiohub.org/public/igem/BBa_J45009/annotation1891631/range1891631/1
role: SO:1000008
role: feature/s mutation

https://synbiohub.org/public/igem/BBa_J45009/annotation1891631/range1891631/1
type: Range
persistent identity: range1891631
display id: range1891631
version: 1
top level: THI3
direction: direction/forward
start: 1791
end: 1791
orientation: inline

stop codon
type: SequenceAnnotation
persistent identity: annotation1961304
display id: annotation1961304
version: 1
top level: THI3
location: https://synbiohub.org/public/igem/BBa_J45009/annotation1961304/range1961304/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_J45009/annotation1961304/range1961304/1
type: Range
persistent identity: range1961304
display id: range1961304
version: 1
top level: THI3
direction: direction/forward
start: 1828
end: 1830
orientation: inline

https://synbiohub.org/public/igem/BBa_J45399_sequence/1
type: Sequence
persistent identity: BBa J45399 sequence
display id: BBa_J45399_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attaaagaggagaaatactagatgaccttggcacccctagacgcctccaaagttaagataactaccacacaacatgcatctaagccaaaaccgaacagtgagttagtgtttggcaagagcttcacggaccacatgttaactgcggaatggacagctgaaaaagggtggggtaccccagagattaaaccttatcaaaatctgtctttagacccttccgcggtggttttccattatgcttttgagctattcgaagggatgaaggcttacagaacggtggacaacaaaattacaatgtttcgtccagatatgaatatgaagcgcatgaataagtctgctcagagaatctgtttgccaacgttcgacccagaagagttgattaccctaattgggaaactgatccagcaagataagtgcttagttcctgaaggaaaaggttactctttatatatcaggcctacattaatcggcactacggccggtttaggggtttccacgcctgatagagccttgctatatgtcatttgctgccctgtgggtccttattacaaaactggatttaaggcggtcagactggaagccactgattatgccacaagagcttggccaggaggctgtggtgacaagaaactaggtgcaaactacgccccctgcgtcctgccacaattgcaagctgcttcaaggggttaccaacaaaatttatggctatttggtccaaataacaacattactgaagtcggcaccatgaatgcttttttcgtgtttaaagatagtaaaacgggcaagaaggaactggttactgctccactagacggtaccattttggaaggtgttactagggattccattttaaatcttgctaaagaaagactcgaaccaagtgaatggaccattagtgaacgctacttcactataggcgaagttactgagagatccaagaacggtgaactacttgaagcctttggttctggtactgctgcgattgtttctcccattaaggaaatcggctggaaaggcgaacaaattaatattccgttgttgcccggcgaacaaaccggtccattggccaaagaagttgcacaatggattaatggaatccaatatggcgagactgagcatggcaattggtcaagggttgttactgatttgaactaataatactagagattaaagaggagaaatactagatgaattctagctatacacagagatatgcactgccgaagtgtatagcaatatcagattatcttttccatcggctcaaccagctgaacatacataccatatttggactctccggagaatttagcatgccgttgctggataaactatacaacattccgaacttacgatgggccggtaattctaatgagttaaatgctgcctacgcagcagatggatactcacgactaaaaggcttgggatgtctcataacaacctttggtgtaggcgaattatcggcaatcaatggcgtggccggatcttacgctgaacatgtaggaatacttcacatagtgggtatgccgccaacaagtgcacaaacgaaacaactactactgcatcatactctgggcaatggtgatttcacggtatttcatagaatagccagtgatgtagcatgctatacaacattgattattgactctgaattatgtgccgacgaagtcgataagtgcatcaaaaaggcttggatagaacagaggccagtatacatgggcatgcctgtcaaccaggtaaatctcccgattgaatcagcaaggcttaatacacctctggatttacaattgcataaaaacgacccagacgtagagaaagaagttatttctcgaatattgagttttatatacaaaagccagaatccggcaatcatcgtagatgcatgtactagtcgacagaatttaatcgaggagactaaagagctttgtaataggcttaaatttccagtttttgttacacctatgggtaagggtacagtaaacgaaacagacccgcaatttgggggc…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0030_sequence/1
type: Sequence
persistent identity: BBa B0030 sequence
display id: BBa_B0030_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J45008_sequence/1
type: Sequence
persistent identity: BBa J45008 sequence
display id: BBa_J45008_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaccttggcacccctagacgcctccaaagttaagataactaccacacaacatgcatctaagccaaaaccgaacagtgagttagtgtttggcaagagcttcacggaccacatgttaactgcggaatggacagctgaaaaagggtggggtaccccagagattaaaccttatcaaaatctgtctttagacccttccgcggtggttttccattatgcttttgagctattcgaagggatgaaggcttacagaacggtggacaacaaaattacaatgtttcgtccagatatgaatatgaagcgcatgaataagtctgctcagagaatctgtttgccaacgttcgacccagaagagttgattaccctaattgggaaactgatccagcaagataagtgcttagttcctgaaggaaaaggttactctttatatatcaggcctacattaatcggcactacggccggtttaggggtttccacgcctgatagagccttgctatatgtcatttgctgccctgtgggtccttattacaaaactggatttaaggcggtcagactggaagccactgattatgccacaagagcttggccaggaggctgtggtgacaagaaactaggtgcaaactacgccccctgcgtcctgccacaattgcaagctgcttcaaggggttaccaacaaaatttatggctatttggtccaaataacaacattactgaagtcggcaccatgaatgcttttttcgtgtttaaagatagtaaaacgggcaagaaggaactggttactgctccactagacggtaccattttggaaggtgttactagggattccattttaaatcttgctaaagaaagactcgaaccaagtgaatggaccattagtgaacgctacttcactataggcgaagttactgagagatccaagaacggtgaactacttgaagcctttggttctggtactgctgcgattgtttctcccattaaggaaatcggctggaaaggcgaacaaattaatattccgttgttgcccggcgaacaaaccggtccattggccaaagaagttgcacaatggattaatggaatccaatatggcgagactgagcatggcaattggtcaagggttgttactgatttgaactaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J45009_sequence/1
type: Sequence
persistent identity: BBa J45009 sequence
display id: BBa_J45009_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaattctagctatacacagagatatgcactgccgaagtgtatagcaatatcagattatcttttccatcggctcaaccagctgaacatacataccatatttggactctccggagaatttagcatgccgttgctggataaactatacaacattccgaacttacgatgggccggtaattctaatgagttaaatgctgcctacgcagcagatggatactcacgactaaaaggcttgggatgtctcataacaacctttggtgtaggcgaattatcggcaatcaatggcgtggccggatcttacgctgaacatgtaggaatacttcacatagtgggtatgccgccaacaagtgcacaaacgaaacaactactactgcatcatactctgggcaatggtgatttcacggtatttcatagaatagccagtgatgtagcatgctatacaacattgattattgactctgaattatgtgccgacgaagtcgataagtgcatcaaaaaggcttggatagaacagaggccagtatacatgggcatgcctgtcaaccaggtaaatctcccgattgaatcagcaaggcttaatacacctctggatttacaattgcataaaaacgacccagacgtagagaaagaagttatttctcgaatattgagttttatatacaaaagccagaatccggcaatcatcgtagatgcatgtactagtcgacagaatttaatcgaggagactaaagagctttgtaataggcttaaatttccagtttttgttacacctatgggtaagggtacagtaaacgaaacagacccgcaatttgggggcgtattcacgggctcgatatcagccccagaagtaagagaagtagttgattttgccgattttatcatcgtcattggttgcatgctctccgaattcagcacgtcaactttccacttccaatataaaactaagaattgtgcgctactatattctacatctgtgaaattgaaaaatgccacatatcctgacttgagcattaaattactactacagaaaatattagcaaatcttgatgaatctaaactgtcttaccaaccaagcgaacaacccagtatgatggttccaagaccttacccagcaggaaatgtcctcttgagacaagaatgggtctggaatgaaatatcccattggttccaaccaggtgacataatcataacagaaactggtgcttctgcatttggagttaaccagaccagatttccggtaaatacactaggtatttcgcaagctctttggggatctgtcggatatacaatgggggcgtgtcttggggcagaatttgctgttcaagagataaacaaggataaattccccgcaactaaacatagagttattctgtttatgggtgacggtgctttccaattgacagttcaagaattatccacaattgttaagtggggattgacaccttatatttttgtgatgaataaccaaggttactctgtggacaggtttttgcatcacaggtcagatgctagttattacgatatccaaccttggaactacttgggattattgcgagtatttggttgcacgaactacgaaacgaaaaaaattattactgttggagaattcagatccatgatcagtgacccaaactttgcgaccaatgacaaaattcggatgatagagattatgctaccaccaagggatgttccacaggctctgcttgacaggtgggtggtagaaaaagaacagagcaaacaagtgcaagaggagaacgaaaattctagcgcagtaaatacgccaactccagaattccaaccacttctaaaaaaaaatcaagttggatactga
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z