LOCUS BBa_J45500 4310 bp DNA linear 14-May-2024 DEFINITION Exponential phase wintergreen odor biosynthetic system ACCESSION BBa_J45500 VERSION BBa_J45500.1 FEATURES Location/Qualifiers misc_feature 3..19 /label=lac O1 promoter 20..25 /label=-35 misc_feature 26..42 /label=lac O1 promoter 43..48 /label=-10 misc_feature 1..54 /label=BBa_R0011 promoter 1..54 /label=BBa_R0011 RBS 64..76 /label=RBS-3\Weak RBS 70..73 /label=RBS misc_feature 64..76 /label=BBa_B0032 RBS 64..76 /label=BBa_B0032 misc_feature 522..522 /label=g -> a to eliminate PstI site misc_feature 385..387 /label=pchA start codon misc_feature 83..85 /label=pchB start codon misc_feature 386..388 /label=pchB stop codon misc_feature 1813..1818 /label=pchA double TAA stop codon CDS 83..388 /label=PchB CDS 385..1818 /label=PchA CDS 83..1818 /label=BBa_J45017 stem_loop 1838..1881 /label=stem_loop misc_feature 1827..1906 /label=BBa_B0010 terminator 1827..1906 /label=BBa_B0010 stem_loop 1922..1941 /label=T7 TE misc_feature 1948..1948 /label=stop polyA_site 1942..1955 /label=polya misc_feature 1915..1955 /label=BBa_B0012 terminator 1915..1955 /label=BBa_B0012 misc_feature 1..1955 /label=BBa_J45320 promoter 2151..2156 /label=-10 promoter 2127..2132 /label=-35 promoter 1964..2162 /label=BBa_J45992 RBS 2171..2182 /label=BBa_B0064 CDS 2192..2808 /label=tetR misc_feature 2809..2842 /label=SsrA misc_feature 2849..2873 /label=Help:Barcodes CDS 2189..2873 /label=BBa_C0040 stem_loop 2893..2936 /label=stem_loop misc_feature 2882..2961 /label=BBa_B0010 terminator 2882..2961 /label=BBa_B0010 stem_loop 2977..2996 /label=T7 TE misc_feature 3003..3003 /label=stop polyA_site 2997..3010 /label=polya misc_feature 2970..3010 /label=BBa_B0012 terminator 2970..3010 /label=BBa_B0012 terminator 2882..3010 /label=BBa_B0015 misc_feature 3019..3037 /label=TetR 1 misc_feature 3019..3072 /label=BBa_R0040 promoter 3038..3043 /label=-35 misc_feature 3044..3062 /label=TetR 2 promoter 3061..3066 /label=-10 promoter 3019..3072 /label=BBa_R0040 misc_feature 2171..3072 /label=BBa_Q04401 promoter 1964..3072 /label=BBa_J45994 RBS 3081..3093 /label=RBS-3\Weak RBS 3087..3090 /label=RBS misc_feature 3081..3093 /label=BBa_B0032 RBS 3081..3093 /label=BBa_B0032 CDS 3100..4173 /label=BMST1 misc_feature 3100..3102 /label=start codon misc_feature 4171..4173 /label=stop codon CDS 3100..4173 /label=BBa_J45004 stem_loop 4193..4236 /label=stem_loop misc_feature 4182..4261 /label=BBa_B0010 terminator 4182..4261 /label=BBa_B0010 stem_loop 4277..4296 /label=T7 TE misc_feature 4303..4303 /label=stop polyA_site 4297..4310 /label=polya misc_feature 4270..4310 /label=BBa_B0012 terminator 4270..4310 /label=BBa_B0012 misc_feature 3081..4310 /label=BBa_J45119 misc_feature 1964..4310 /label=BBa_J45181 ORIGIN 1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta 61 gagtcacaca ggaaagtact agatgaaaac tcccgaagac tgcaccggcc tggcggacat 121 ccgcgaggcc atcgaccgga tcgacctgga tatcgtccag gccctcggcc gccgcatgga 181 ctacgtcaag gcggcgtcgc gcttcaaggc cagcgaggcg gcgattccgg cgcccgagcg 241 ggtcgccgcg atgctccccg agcgcgcccg ctgggccgag gaaaacggac tcgacgcgcc 301 cttcgtcgag ggactgttcg cgcagatcat ccactggtac atcgccgagc agatcaagta 361 ctggcgccag acacggggtg ccgcatgagc cggctggcgc ccctgagcca gtgcctgcac 421 gccttgcgcg gcaccttcga gcgcgccatc ggccaggcgc aggcgctcga tcgtccggtg 481 ctggtggcgg catcgttcga gatcgaccca ttggacccgc tacaggtatt cggtgcctgg 541 gacgaccggc aaacgccctg cctgtactgg gaacagcccg agctggcgtt cttcgcctgg 601 ggctgcgccc tggagctgca aggccacggc gaacagcgct tcgcccggat cgaggaaaac 661 tggcaattgc tctgcgccga cgccgtggtc gagggcccgc tggcgccgcg cctgtgcggc 721 ggattccgct tcgatccgcg cggcccgcgc gaggaacact ggcaagcctt cgccgatgcc 781 agcctgatgc tcgccggcat caccgtgctg cgcgagggcg aacgctaccg ggtactctgc 841 caacacctgg ccaagcccgg cgaagatgcc ctggccctgg ccgcctacca ctgctcggcg 901 ctactgcgcc tgaggcagcc ggccagacgc cggccctcgg ggccgaccgc tggcgcgcag 961 ggcgacgctt cggcgcagga gcgcaggcaa tgggaagcca aggtgagcga cgcggtaagc 1021 agtgtccgcc agggacgctt cggcaaggtc gtgctggccc gcacccaggc ccggcctctc 1081 ggcgacatcg agccgtggca ggtcatcgaa cacctgcgtc tgcaacatgc cgacgcccag 1141 ctgttcgcct gtcgccgcgg caacgcctgc ttcctcggcg cctccccgga acgcctggtc 1201 cgcattcgcg ccggcgaggc actcacccat gccctggccg ggaccatcgc ccgcggcggc 1261 gatgcccagg aagatgcgcg gctcggacag gccctgctgg acagcgccaa ggacaggcac 1321 gaacaccagt tggtggtgga ggcgatccgt acggccctgg aacccttcag cgaggtgctg 1381 gaaatccccg atgcgcccgg cctgaaacga ctggcgcgag tccagcacct gaacacgccg 1441 atccgcgccc gcctcgctga cgcaggcggc atcctgcggc tgctacaagc gctgcatccg 1501 acccccgcgg tgggcggcta cccacgcagc gcggcgctgg actacatccg ccagcacgaa 1561 gggatggacc gcggctggta cgccgcgccg ctgggctggc tcgacggcga aggcaacggc 1621 gatttcctgg tggcgctgcg ctcggccctg ctcacgccgg gccggggcta cctgttcgcc 1681 ggctgcggtc tggtaggcga ttcggaaccg gcccacgagt atcgcgaaac ctgccttaag 1741 ctcagtgcca tgcgggaagc tctatccgcc ataggcggcc tggacgaagt gcccttgcag 1801 cgcggcgtcg cctaataata ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa 1861 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac 1921 tggctcacct tcgggtgggc ctttctgcgt ttatatacta gagctggcac aggaacgtta 1981 tccggacgtt cagttccacc agacccgcga gcattaattc ttgcctccag ggcgcggtag 2041 tggcgccctg tcaatttccc ttccttatta gccgcttacg gaatgttctt aaaacattca 2101 cttttgctta tgttttcgct gatatcccga gcggtttcaa aattgtgatc tatatttaac 2161 aatactagag aaagagggga aatactagat gtccagatta gataaaagta aagtgattaa 2221 cagcgcatta gagctgctta atgaggtcgg aatcgaaggt ttaacaaccc gtaaactcgc 2281 ccagaagcta ggtgtagagc agcctacatt gtattggcat gtaaaaaata agcgggcttt 2341 gctcgacgcc ttagccattg agatgttaga taggcaccat actcactttt gccctttaga 2401 aggggaaagc tggcaagatt ttttacgtaa taacgctaaa agttttagat gtgctttact 2461 aagtcatcgc gatggagcaa aagtacattt aggtacacgg cctacagaaa aacagtatga 2521 aactctcgaa aatcaattag cctttttatg ccaacaaggt ttttcactag agaatgcatt 2581 atatgcactc agcgctgtgg ggcattttac tttaggttgc gtattggaag atcaagagca 2641 tcaagtcgct aaagaagaaa gggaaacacc tactactgat agtatgccgc cattattacg 2701 acaagctatc gaattatttg atcaccaagg tgcagagcca gccttcttat tcggccttga 2761 attgatcata tgcggattag aaaaacaact taaatgtgaa agtgggtccg ctgcaaacga 2821 cgaaaactac gctttagtag cttaataaca ctgatagtgc tagtgtagat cactactaga 2881 gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt 2941 tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc 3001 tgcgtttata tactagagtc cctatcagtg atagagattg acatccctat cagtgataga 3061 gatactgagc actactagag tcacacagga aagtactaga tggaagttgt tgaagttctt 3121 cacatgaatg gaggaaatgg agacagtagc tatgcaaaca attctttggt tcagcaaaag 3181 gtgattctca tgacaaagcc aataactgag caagccatga ttgatctcta cagcagcctc 3241 tttccagaaa ccttatgcat tgcagatttg ggttgttctt tgggagctaa cactttcttg 3301 gtggtctcac agcttgttaa aatagtagaa aaagaacgaa aaaagcatgg ttttaagtct 3361 ccagagtttt attttcactt caatgatctt cctggcaatg attttaatac actttttcag 3421 tcactggggg catttcaaga agatttgaga aagcatatag gggaaagctt tggtccatgt 3481 tttttcagtg gagtgcctgg ttcattttat actagacttt tcccttccaa aagtttacat 3541 tttgtttact cctcctacag tctcatgtgg ctatctcagg tgcctaatgg gattgaaaat 3601 aacaagggaa acatttacat ggcaagaaca agccctctaa gtgttattaa agcatactac 3661 aagcaatatg aaatagattt ttcaaatttt ctcaagtacc gttcagagga attgatgaaa 3721 ggtggaaaga tggtgttaac actcctaggt agagaaagtg aggatcctac tagcaaagaa 3781 tgctgttaca tttgggagct tctagccatg gccctcaata agttggttga agagggattg 3841 ataaaagaag agaaagtaga tgcattcaat attcctcaat acacaccatc accagcagaa 3901 gtaaagtaca tagttgagaa ggaaggatca ttcaccatta atcgcttgga aacatcaaga 3961 gttcattgga atgcttctaa taatgagaag aatggtggtt acaatgtgtc aaggtgcatg 4021 agagctgtgg ctgagccttt gcttgtcagc cactttgaca aggaattgat ggatttagtg 4081 ttccacaagt acgaagagat tgtttctgat tgcatgtcca aagagaatac tgagtttata 4141 aatgtcatca tctccttgac caaaataaat taatactaga gccaggcatc aaataaaacg 4201 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct 4261 ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata //