##gff-version 3
##sequence-region BBa_J45500 1 4310
BBa_J45500	.	engineered_region	1964	4310	.	+	0	ID=BBa_J45181;Name=BBa_J45181
BBa_J45500	.	promoter	1964	3072	.	+	0	ID=BBa_J45994;Name=BBa_J45994;Parent=BBa_J45181
BBa_J45500	.	promoter	1964	2162	.	+	0	ID=BBa_J45992;Name=BBa_J45992;Parent=BBa_J45994
BBa_J45500	.	promoter	2151	2156	.	+	0	ID=annotation1883645;Name=-10;Parent=BBa_J45992
BBa_J45500	.	promoter	2127	2132	.	+	0	ID=annotation1883646;Name=-35;Parent=BBa_J45992
BBa_J45500	.	engineered_region	2171	3072	.	+	0	ID=BBa_Q04401;Name=BBa_Q04401;Parent=BBa_J45994
BBa_J45500	.	CDS	2189	2873	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_Q04401
BBa_J45500	.	tag	2809	2842	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_J45500	.	engineered_tag	2849	2873	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_J45500	.	CDS	2192	2808	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_J45500	.	promoter	3019	3072	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_Q04401
BBa_J45500	.	engineered_region	3019	3072	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_J45500	.	promoter	3038	3043	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_J45500	.	non_covalent_binding_site	3019	3037	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_J45500	.	promoter	3061	3066	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_J45500	.	non_covalent_binding_site	3044	3062	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_J45500	.	terminator	2882	3010	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_Q04401
BBa_J45500	.	terminator	2970	3010	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_J45500	.	stem_loop	2977	2996	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J45500	.	stop_codon	3003	3003	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J45500	.	polyA_site	2997	3010	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J45500	.	engineered_region	2970	3010	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J45500	.	terminator	2882	2961	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_J45500	.	stem_loop	2893	2936	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J45500	.	engineered_region	2882	2961	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J45500	.	ribosome_entry_site	2171	2182	.	+	0	ID=BBa_B0064;Name=BBa_B0064;Parent=BBa_Q04401
BBa_J45500	.	engineered_region	3081	4310	.	+	0	ID=BBa_J45119;Name=BBa_J45119;Parent=BBa_J45181
BBa_J45500	.	terminator	4182	4261	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J45119
BBa_J45500	.	stem_loop	4193	4236	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J45500	.	engineered_region	4182	4261	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J45500	.	CDS	3100	4173	.	+	0	ID=BBa_J45004;Name=BBa_J45004;Parent=BBa_J45119
BBa_J45500	.	start_codon	3100	3102	.	+	0	ID=annotation1880519;Name=start codon;Parent=BBa_J45004
BBa_J45500	.	CDS	3100	4173	.	+	0	ID=annotation1880517;Name=BMST1;Parent=BBa_J45004
BBa_J45500	.	stop_codon	4171	4173	.	+	0	ID=annotation1880520;Name=stop codon;Parent=BBa_J45004
BBa_J45500	.	ribosome_entry_site	3081	3093	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_J45119
BBa_J45500	.	engineered_region	3081	3093	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_J45500	.	ribosome_entry_site	3087	3090	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_J45500	.	ribosome_entry_site	3081	3093	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_J45500	.	terminator	4270	4310	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J45119
BBa_J45500	.	stem_loop	4277	4296	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J45500	.	stop_codon	4303	4303	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J45500	.	polyA_site	4297	4310	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J45500	.	engineered_region	4270	4310	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J45500	.	engineered_region	1	1955	.	+	0	ID=BBa_J45320;Name=BBa_J45320
BBa_J45500	.	promoter	1	54	.	+	0	ID=BBa_R0011;Name=BBa_R0011;Parent=BBa_J45320
BBa_J45500	.	promoter	43	48	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_J45500	.	promoter	20	25	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_J45500	.	operator	3	19	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_J45500	.	engineered_region	1	54	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_J45500	.	operator	26	42	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_J45500	.	terminator	1915	1955	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J45320
BBa_J45500	.	stem_loop	1922	1941	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J45500	.	stop_codon	1948	1948	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J45500	.	polyA_site	1942	1955	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J45500	.	engineered_region	1915	1955	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J45500	.	CDS	83	1818	.	+	0	ID=BBa_J45017;Name=BBa_J45017;Parent=BBa_J45320
BBa_J45500	.	point_mutation	522	522	.	+	0	ID=annotation1897453;Name=g -> a to eliminate PstI site;Parent=BBa_J45017
BBa_J45500	.	CDS	385	1818	.	+	0	ID=annotation1961297;Name=PchA;Parent=BBa_J45017
BBa_J45500	.	stop_codon	386	388	.	+	0	ID=annotation1897456;Name=pchB stop codon;Parent=BBa_J45017
BBa_J45500	.	stop_codon	1813	1818	.	+	0	ID=annotation1897457;Name=pchA double TAA stop codon;Parent=BBa_J45017
BBa_J45500	.	start_codon	385	387	.	+	0	ID=annotation1897454;Name=pchA start codon;Parent=BBa_J45017
BBa_J45500	.	CDS	83	388	.	+	0	ID=annotation1961296;Name=PchB;Parent=BBa_J45017
BBa_J45500	.	start_codon	83	85	.	+	0	ID=annotation1897455;Name=pchB start codon;Parent=BBa_J45017
BBa_J45500	.	ribosome_entry_site	64	76	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_J45320
BBa_J45500	.	engineered_region	64	76	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_J45500	.	ribosome_entry_site	70	73	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_J45500	.	ribosome_entry_site	64	76	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_J45500	.	terminator	1827	1906	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J45320
BBa_J45500	.	stem_loop	1838	1881	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J45500	.	engineered_region	1827	1906	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
>BBa_J45500
aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagtcacacaggaaagtac
tagatgaaaactcccgaagactgcaccggcctggcggacatccgcgaggccatcgaccggatcgacctggatatcgtcc
aggccctcggccgccgcatggactacgtcaaggcggcgtcgcgcttcaaggccagcgaggcggcgattccggcgcccga
gcgggtcgccgcgatgctccccgagcgcgcccgctgggccgaggaaaacggactcgacgcgcccttcgtcgagggactg
ttcgcgcagatcatccactggtacatcgccgagcagatcaagtactggcgccagacacggggtgccgcatgagccggct
ggcgcccctgagccagtgcctgcacgccttgcgcggcaccttcgagcgcgccatcggccaggcgcaggcgctcgatcgt
ccggtgctggtggcggcatcgttcgagatcgacccattggacccgctacaggtattcggtgcctgggacgaccggcaaa
cgccctgcctgtactgggaacagcccgagctggcgttcttcgcctggggctgcgccctggagctgcaaggccacggcga
acagcgcttcgcccggatcgaggaaaactggcaattgctctgcgccgacgccgtggtcgagggcccgctggcgccgcgc
ctgtgcggcggattccgcttcgatccgcgcggcccgcgcgaggaacactggcaagccttcgccgatgccagcctgatgc
tcgccggcatcaccgtgctgcgcgagggcgaacgctaccgggtactctgccaacacctggccaagcccggcgaagatgc
cctggccctggccgcctaccactgctcggcgctactgcgcctgaggcagccggccagacgccggccctcggggccgacc
gctggcgcgcagggcgacgcttcggcgcaggagcgcaggcaatgggaagccaaggtgagcgacgcggtaagcagtgtcc
gccagggacgcttcggcaaggtcgtgctggcccgcacccaggcccggcctctcggcgacatcgagccgtggcaggtcat
cgaacacctgcgtctgcaacatgccgacgcccagctgttcgcctgtcgccgcggcaacgcctgcttcctcggcgcctcc
ccggaacgcctggtccgcattcgcgccggcgaggcactcacccatgccctggccgggaccatcgcccgcggcggcgatg
cccaggaagatgcgcggctcggacaggccctgctggacagcgccaaggacaggcacgaacaccagttggtggtggaggc
gatccgtacggccctggaacccttcagcgaggtgctggaaatccccgatgcgcccggcctgaaacgactggcgcgagtc
cagcacctgaacacgccgatccgcgcccgcctcgctgacgcaggcggcatcctgcggctgctacaagcgctgcatccga
cccccgcggtgggcggctacccacgcagcgcggcgctggactacatccgccagcacgaagggatggaccgcggctggta
cgccgcgccgctgggctggctcgacggcgaaggcaacggcgatttcctggtggcgctgcgctcggccctgctcacgccg
ggccggggctacctgttcgccggctgcggtctggtaggcgattcggaaccggcccacgagtatcgcgaaacctgcctta
agctcagtgccatgcgggaagctctatccgccataggcggcctggacgaagtgcccttgcagcgcggcgtcgcctaata
atactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggt
gaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagctggcacaggaa
cgttatccggacgttcagttccaccagacccgcgagcattaattcttgcctccagggcgcggtagtggcgccctgtcaa
tttcccttccttattagccgcttacggaatgttcttaaaacattcacttttgcttatgttttcgctgatatcccgagcg
gtttcaaaattgtgatctatatttaacaatactagagaaagaggggaaatactagatgtccagattagataaaagtaaa
gtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctag
gtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttaga
taggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttaga
tgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcg
aaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattt
tactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatg
ccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattga
tcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagctta
ataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactggg
cctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctg
cgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagt
cacacaggaaagtactagatggaagttgttgaagttcttcacatgaatggaggaaatggagacagtagctatgcaaaca
attctttggttcagcaaaaggtgattctcatgacaaagccaataactgagcaagccatgattgatctctacagcagcct
ctttccagaaaccttatgcattgcagatttgggttgttctttgggagctaacactttcttggtggtctcacagcttgtt
aaaatagtagaaaaagaacgaaaaaagcatggttttaagtctccagagttttattttcacttcaatgatcttcctggca
atgattttaatacactttttcagtcactgggggcatttcaagaagatttgagaaagcatataggggaaagctttggtcc
atgttttttcagtggagtgcctggttcattttatactagacttttcccttccaaaagtttacattttgtttactcctcc
tacagtctcatgtggctatctcaggtgcctaatgggattgaaaataacaagggaaacatttacatggcaagaacaagcc
ctctaagtgttattaaagcatactacaagcaatatgaaatagatttttcaaattttctcaagtaccgttcagaggaatt
gatgaaaggtggaaagatggtgttaacactcctaggtagagaaagtgaggatcctactagcaaagaatgctgttacatt
tgggagcttctagccatggccctcaataagttggttgaagagggattgataaaagaagagaaagtagatgcattcaata
ttcctcaatacacaccatcaccagcagaagtaaagtacatagttgagaaggaaggatcattcaccattaatcgcttgga
aacatcaagagttcattggaatgcttctaataatgagaagaatggtggttacaatgtgtcaaggtgcatgagagctgtg
gctgagcctttgcttgtcagccactttgacaaggaattgatggatttagtgttccacaagtacgaagagattgtttctg
attgcatgtccaaagagaatactgagtttataaatgtcatcatctccttgaccaaaataaattaatactagagccaggc
atcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctacta
gagtcacactggctcaccttcgggtgggcctttctgcgtttata