LOCUS BBa_J45600 5031 bp DNA linear 22-Dec-2024 DEFINITION Stationary phase banana odor biosynthetic system ACCESSION BBa_J45600 VERSION BBa_J45600.1 FEATURES Location/Qualifiers promoter 188..193 /label=-10 promoter 164..169 /label=-35 promoter 1..199 /label=BBa_J45992 RBS 208..222 /label=RBS-1\Strong RBS 215..219 /label=RBS misc_feature 208..222 /label=BBa_B0030 RBS 208..222 /label=BBa_B0030 CDS 229..1809 /label=ATF1 misc_feature 1376..1376 /label=t -> c misc_feature 1804..1809 /label=double TAA stop codon misc_feature 642..642 /label=a->g to eliminate EcoRI site CDS 229..1809 /label=BBa_J45014 stem_loop 1829..1872 /label=stem_loop misc_feature 1818..1897 /label=BBa_B0010 terminator 1818..1897 /label=BBa_B0010 stem_loop 1913..1932 /label=T7 TE misc_feature 1939..1939 /label=stop polyA_site 1933..1946 /label=polya misc_feature 1906..1946 /label=BBa_B0012 terminator 1906..1946 /label=BBa_B0012 misc_feature 1957..1973 /label=lac O1 promoter 1974..1979 /label=-35 misc_feature 1980..1996 /label=lac O1 promoter 1997..2002 /label=-10 misc_feature 1955..2008 /label=BBa_R0011 promoter 1955..2008 /label=BBa_R0011 RBS 2018..2032 /label=RBS-1\Strong RBS 2025..2029 /label=RBS misc_feature 2018..2032 /label=BBa_B0030 RBS 2018..2032 /label=BBa_B0030 misc_feature 2039..2041 /label=start codon misc_feature 3167..3172 /label=double TAA stop codon misc_feature 2809..2809 /label=a->g to eliminate SpeI CDS 2039..3172 /label=BAT2 CDS 2039..3172 /label=BBa_J45008 RBS 3181..3195 /label=RBS-1\Strong RBS 3188..3192 /label=RBS misc_feature 3181..3195 /label=BBa_B0030 RBS 3181..3195 /label=BBa_B0030 misc_feature 3207..3207 /label=t->c to eliminate EcoRI, to do misc_feature 4107..4107 /label=a->g to eliminate EcoRI, to do misc_feature 4806..4806 /label=a->g to eliminate EcoRI, to do misc_feature 4992..4992 /label=a->g to eliminate EcoRI, to do misc_feature 3900..3900 /label=t->c to eliminate SpeI, to do CDS 3202..5031 /label=THI3 misc_feature 3202..3204 /label=start codon misc_feature 5029..5031 /label=stop codon CDS 3202..5031 /label=BBa_J45009 ORIGIN 1 ctggcacagg aacgttatcc ggacgttcag ttccaccaga cccgcgagca ttaattcttg 61 cctccagggc gcggtagtgg cgccctgtca atttcccttc cttattagcc gcttacggaa 121 tgttcttaaa acattcactt ttgcttatgt tttcgctgat atcccgagcg gtttcaaaat 181 tgtgatctat atttaacaat actagagatt aaagaggaga aatactagat gaatgaaatc 241 gatgagaaaa atcaggcccc cgtgcaacaa gaatgcctga aagagatgat tcagaatggg 301 catgctcggc gtatgggatc tgttgaagat ctgtatgttg ctctcaacag acaaaactta 361 tatcgaaact tctgcacata tggagaattg agtgattact gtactaggga tcagctcaca 421 ttagctttga gggaaatctg cctgaaaaat ccaactcttt tacatattgt tctaccaaca 481 agatggccaa atcatgaaaa ttattatcgc agttccgaat actattcacg gccacatcca 541 gtgcatgatt atatttcagt attacaagaa ttgaaactga gtggtgtggt tctcaatgaa 601 caacctgagt acagtgcagt aatgaagcaa atattagaag agttcaaaaa tagtaagggt 661 tcctatactg caaaaatttt taaacttact accactttga ctattcctta ctttggacca 721 acaggaccga gttggcggct aatttgtctt ccagaagagc acacagaaaa gtggaaaaaa 781 tttatctttg tatctaatca ttgcatgtct gatggtcggt cttcgatcca cttttttcat 841 gatttaagag acgaattaaa taatattaaa actccaccaa aaaaattaga ttacattttc 901 aagtacgagg aggattacca attattgagg aaacttccag aaccgatcga aaaggtgata 961 gactttagac caccgtactt gtttattccg aagtcacttc tttcgggttt catctacaat 1021 catttgagat tttcttcaaa aggtgtctgt atgagaatgg atgatgtgga aaaaaccgat 1081 gatgttgtca ccgagatcat caatatttca ccaacagaat ttcaagcgat taaagcaaat 1141 attaaatcaa atatccaagg taagtgtact atcactccgt ttttacatgt ttgttggttt 1201 gtatctcttc ataaatgggg taaatttttc aaaccattga acttcgaatg gcttacggat 1261 atttttatcc ccgcagattg ccgctcacaa ctaccagatg atgatgaaat gagacagatg 1321 tacagatatg gcgctaacgt tggatttatt gacttcaccc cctggataag cgaatctgac 1381 atgaatgata acaaagaaaa tttttggcca cttattgagc actaccatga agtaatttcg 1441 gaagctttaa gaaataaaaa gcatctccat ggcttagggt tcaatataca aggcttcgtt 1501 caaaaatatg tgaacattga caaggtaatg tgcgatcgtg ccatcgggaa aagacgcgga 1561 ggtacattgt taagcaatgt aggtctgttt aatcagttag aggagcccga tgccaaatat 1621 tctatatgcg atttggcatt tggccaattt caaggatcct ggcaccaagc attttccttg 1681 ggtgtttgtt cgactaatgt aaaggggatg aatattgttg ttgcttcaac aaagaatgtt 1741 gttggtagtc aagaatctct cgaagagctt tgctccattt acaaagctct ccttttaggc 1801 ccttaataat actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc 1861 ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc 1921 ttcgggtggg cctttctgcg tttatatact agagaattgt gagcggataa caattgacat 1981 tgtgagcgga taacaagata ctgagcacat actagagatt aaagaggaga aatactagat 2041 gaccttggca cccctagacg cctccaaagt taagataact accacacaac atgcatctaa 2101 gccaaaaccg aacagtgagt tagtgtttgg caagagcttc acggaccaca tgttaactgc 2161 ggaatggaca gctgaaaaag ggtggggtac cccagagatt aaaccttatc aaaatctgtc 2221 tttagaccct tccgcggtgg ttttccatta tgcttttgag ctattcgaag ggatgaaggc 2281 ttacagaacg gtggacaaca aaattacaat gtttcgtcca gatatgaata tgaagcgcat 2341 gaataagtct gctcagagaa tctgtttgcc aacgttcgac ccagaagagt tgattaccct 2401 aattgggaaa ctgatccagc aagataagtg cttagttcct gaaggaaaag gttactcttt 2461 atatatcagg cctacattaa tcggcactac ggccggttta ggggtttcca cgcctgatag 2521 agccttgcta tatgtcattt gctgccctgt gggtccttat tacaaaactg gatttaaggc 2581 ggtcagactg gaagccactg attatgccac aagagcttgg ccaggaggct gtggtgacaa 2641 gaaactaggt gcaaactacg ccccctgcgt cctgccacaa ttgcaagctg cttcaagggg 2701 ttaccaacaa aatttatggc tatttggtcc aaataacaac attactgaag tcggcaccat 2761 gaatgctttt ttcgtgttta aagatagtaa aacgggcaag aaggaactgg ttactgctcc 2821 actagacggt accattttgg aaggtgttac tagggattcc attttaaatc ttgctaaaga 2881 aagactcgaa ccaagtgaat ggaccattag tgaacgctac ttcactatag gcgaagttac 2941 tgagagatcc aagaacggtg aactacttga agcctttggt tctggtactg ctgcgattgt 3001 ttctcccatt aaggaaatcg gctggaaagg cgaacaaatt aatattccgt tgttgcccgg 3061 cgaacaaacc ggtccattgg ccaaagaagt tgcacaatgg attaatggaa tccaatatgg 3121 cgagactgag catggcaatt ggtcaagggt tgttactgat ttgaactaat aatactagag 3181 attaaagagg agaaatacta gatgaattct agctatacac agagatatgc actgccgaag 3241 tgtatagcaa tatcagatta tcttttccat cggctcaacc agctgaacat acataccata 3301 tttggactct ccggagaatt tagcatgccg ttgctggata aactatacaa cattccgaac 3361 ttacgatggg ccggtaattc taatgagtta aatgctgcct acgcagcaga tggatactca 3421 cgactaaaag gcttgggatg tctcataaca acctttggtg taggcgaatt atcggcaatc 3481 aatggcgtgg ccggatctta cgctgaacat gtaggaatac ttcacatagt gggtatgccg 3541 ccaacaagtg cacaaacgaa acaactacta ctgcatcata ctctgggcaa tggtgatttc 3601 acggtatttc atagaatagc cagtgatgta gcatgctata caacattgat tattgactct 3661 gaattatgtg ccgacgaagt cgataagtgc atcaaaaagg cttggataga acagaggcca 3721 gtatacatgg gcatgcctgt caaccaggta aatctcccga ttgaatcagc aaggcttaat 3781 acacctctgg atttacaatt gcataaaaac gacccagacg tagagaaaga agttatttct 3841 cgaatattga gttttatata caaaagccag aatccggcaa tcatcgtaga tgcatgtact 3901 agtcgacaga atttaatcga ggagactaaa gagctttgta ataggcttaa atttccagtt 3961 tttgttacac ctatgggtaa gggtacagta aacgaaacag acccgcaatt tgggggcgta 4021 ttcacgggct cgatatcagc cccagaagta agagaagtag ttgattttgc cgattttatc 4081 atcgtcattg gttgcatgct ctccgaattc agcacgtcaa ctttccactt ccaatataaa 4141 actaagaatt gtgcgctact atattctaca tctgtgaaat tgaaaaatgc cacatatcct 4201 gacttgagca ttaaattact actacagaaa atattagcaa atcttgatga atctaaactg 4261 tcttaccaac caagcgaaca acccagtatg atggttccaa gaccttaccc agcaggaaat 4321 gtcctcttga gacaagaatg ggtctggaat gaaatatccc attggttcca accaggtgac 4381 ataatcataa cagaaactgg tgcttctgca tttggagtta accagaccag atttccggta 4441 aatacactag gtatttcgca agctctttgg ggatctgtcg gatatacaat gggggcgtgt 4501 cttggggcag aatttgctgt tcaagagata aacaaggata aattccccgc aactaaacat 4561 agagttattc tgtttatggg tgacggtgct ttccaattga cagttcaaga attatccaca 4621 attgttaagt ggggattgac accttatatt tttgtgatga ataaccaagg ttactctgtg 4681 gacaggtttt tgcatcacag gtcagatgct agttattacg atatccaacc ttggaactac 4741 ttgggattat tgcgagtatt tggttgcacg aactacgaaa cgaaaaaaat tattactgtt 4801 ggagaattca gatccatgat cagtgaccca aactttgcga ccaatgacaa aattcggatg 4861 atagagatta tgctaccacc aagggatgtt ccacaggctc tgcttgacag gtgggtggta 4921 gaaaaagaac agagcaaaca agtgcaagag gagaacgaaa attctagcgc agtaaatacg 4981 ccaactccag aattccaacc acttctaaaa aaaaatcaag ttggatactg a //