##gff-version 3
##sequence-region BBa_J45600 1 5031
BBa_J45600	.	CDS	229	1809	.	+	0	ID=BBa_J45014;Name=BBa_J45014
BBa_J45600	.	stop_codon	1804	1809	.	+	0	ID=annotation1961302;Name=double TAA stop codon;Parent=BBa_J45014
BBa_J45600	.	CDS	229	1809	.	+	0	ID=annotation1961300;Name=ATF1;Parent=BBa_J45014
BBa_J45600	.	sequence_alteration	1376	1376	.	+	0	ID=annotation1961301;Name=t -> c;Parent=BBa_J45014
BBa_J45600	.	point_mutation	642	642	.	+	0	ID=annotation1961305;Name=a->g to eliminate EcoRI site;Parent=BBa_J45014
BBa_J45600	.	ribosome_entry_site	208	222	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_J45600	.	ribosome_entry_site	208	222	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_J45600	.	engineered_region	208	222	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_J45600	.	ribosome_entry_site	215	219	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_J45600	.	ribosome_entry_site	3181	3195	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_J45600	.	ribosome_entry_site	3181	3195	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_J45600	.	engineered_region	3181	3195	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_J45600	.	ribosome_entry_site	3188	3192	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_J45600	.	promoter	1	199	.	+	0	ID=BBa_J45992;Name=BBa_J45992
BBa_J45600	.	promoter	164	169	.	+	0	ID=annotation1883646;Name=-35;Parent=BBa_J45992
BBa_J45600	.	promoter	188	193	.	+	0	ID=annotation1883645;Name=-10;Parent=BBa_J45992
BBa_J45600	.	terminator	1818	1897	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_J45600	.	stem_loop	1829	1872	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J45600	.	engineered_region	1818	1897	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J45600	.	CDS	2039	3172	.	+	0	ID=BBa_J45008;Name=BBa_J45008
BBa_J45600	.	stop_codon	3167	3172	.	+	0	ID=annotation1883625;Name=double TAA stop codon;Parent=BBa_J45008
BBa_J45600	.	point_mutation	2809	2809	.	+	0	ID=annotation1894553;Name=a->g to eliminate SpeI;Parent=BBa_J45008
BBa_J45600	.	CDS	2039	3172	.	+	0	ID=annotation1961299;Name=BAT2;Parent=BBa_J45008
BBa_J45600	.	start_codon	2039	2041	.	+	0	ID=annotation1883624;Name=start codon;Parent=BBa_J45008
BBa_J45600	.	ribosome_entry_site	2018	2032	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_J45600	.	ribosome_entry_site	2018	2032	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_J45600	.	engineered_region	2018	2032	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_J45600	.	ribosome_entry_site	2025	2029	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_J45600	.	terminator	1906	1946	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_J45600	.	stop_codon	1939	1939	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J45600	.	stem_loop	1913	1932	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J45600	.	engineered_region	1906	1946	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J45600	.	polyA_site	1933	1946	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J45600	.	promoter	1955	2008	.	+	0	ID=BBa_R0011;Name=BBa_R0011
BBa_J45600	.	promoter	1997	2002	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_J45600	.	operator	1980	1996	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_J45600	.	promoter	1974	1979	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_J45600	.	operator	1957	1973	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_J45600	.	engineered_region	1955	2008	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_J45600	.	CDS	3202	5031	.	+	0	ID=BBa_J45009;Name=BBa_J45009
BBa_J45600	.	point_mutation	4107	4107	.	+	0	ID=annotation1891629;Name=a->g to eliminate EcoRI, to do;Parent=BBa_J45009
BBa_J45600	.	point_mutation	4992	4992	.	+	0	ID=annotation1891631;Name=a->g to eliminate EcoRI, to do;Parent=BBa_J45009
BBa_J45600	.	start_codon	3202	3204	.	+	0	ID=annotation1961303;Name=start codon;Parent=BBa_J45009
BBa_J45600	.	CDS	3202	5031	.	+	0	ID=annotation1961298;Name=THI3;Parent=BBa_J45009
BBa_J45600	.	stop_codon	5029	5031	.	+	0	ID=annotation1961304;Name=stop codon;Parent=BBa_J45009
BBa_J45600	.	point_mutation	3207	3207	.	+	0	ID=annotation1891628;Name=t->c to eliminate EcoRI, to do;Parent=BBa_J45009
BBa_J45600	.	point_mutation	3900	3900	.	+	0	ID=annotation1891632;Name=t->c to eliminate SpeI, to do;Parent=BBa_J45009
BBa_J45600	.	point_mutation	4806	4806	.	+	0	ID=annotation1891630;Name=a->g to eliminate EcoRI, to do;Parent=BBa_J45009
>BBa_J45600
ctggcacaggaacgttatccggacgttcagttccaccagacccgcgagcattaattcttgcctccagggcgcggtagtg
gcgccctgtcaatttcccttccttattagccgcttacggaatgttcttaaaacattcacttttgcttatgttttcgctg
atatcccgagcggtttcaaaattgtgatctatatttaacaatactagagattaaagaggagaaatactagatgaatgaa
atcgatgagaaaaatcaggcccccgtgcaacaagaatgcctgaaagagatgattcagaatgggcatgctcggcgtatgg
gatctgttgaagatctgtatgttgctctcaacagacaaaacttatatcgaaacttctgcacatatggagaattgagtga
ttactgtactagggatcagctcacattagctttgagggaaatctgcctgaaaaatccaactcttttacatattgttcta
ccaacaagatggccaaatcatgaaaattattatcgcagttccgaatactattcacggccacatccagtgcatgattata
tttcagtattacaagaattgaaactgagtggtgtggttctcaatgaacaacctgagtacagtgcagtaatgaagcaaat
attagaagagttcaaaaatagtaagggttcctatactgcaaaaatttttaaacttactaccactttgactattccttac
tttggaccaacaggaccgagttggcggctaatttgtcttccagaagagcacacagaaaagtggaaaaaatttatctttg
tatctaatcattgcatgtctgatggtcggtcttcgatccacttttttcatgatttaagagacgaattaaataatattaa
aactccaccaaaaaaattagattacattttcaagtacgaggaggattaccaattattgaggaaacttccagaaccgatc
gaaaaggtgatagactttagaccaccgtacttgtttattccgaagtcacttctttcgggtttcatctacaatcatttga
gattttcttcaaaaggtgtctgtatgagaatggatgatgtggaaaaaaccgatgatgttgtcaccgagatcatcaatat
ttcaccaacagaatttcaagcgattaaagcaaatattaaatcaaatatccaaggtaagtgtactatcactccgttttta
catgtttgttggtttgtatctcttcataaatggggtaaatttttcaaaccattgaacttcgaatggcttacggatattt
ttatccccgcagattgccgctcacaactaccagatgatgatgaaatgagacagatgtacagatatggcgctaacgttgg
atttattgacttcaccccctggataagcgaatctgacatgaatgataacaaagaaaatttttggccacttattgagcac
taccatgaagtaatttcggaagctttaagaaataaaaagcatctccatggcttagggttcaatatacaaggcttcgttc
aaaaatatgtgaacattgacaaggtaatgtgcgatcgtgccatcgggaaaagacgcggaggtacattgttaagcaatgt
aggtctgtttaatcagttagaggagcccgatgccaaatattctatatgcgatttggcatttggccaatttcaaggatcc
tggcaccaagcattttccttgggtgtttgttcgactaatgtaaaggggatgaatattgttgttgcttcaacaaagaatg
ttgttggtagtcaagaatctctcgaagagctttgctccatttacaaagctctccttttaggcccttaataatactagag
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctct
ctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagaattgtgagcggataacaatt
gacattgtgagcggataacaagatactgagcacatactagagattaaagaggagaaatactagatgaccttggcacccc
tagacgcctccaaagttaagataactaccacacaacatgcatctaagccaaaaccgaacagtgagttagtgtttggcaa
gagcttcacggaccacatgttaactgcggaatggacagctgaaaaagggtggggtaccccagagattaaaccttatcaa
aatctgtctttagacccttccgcggtggttttccattatgcttttgagctattcgaagggatgaaggcttacagaacgg
tggacaacaaaattacaatgtttcgtccagatatgaatatgaagcgcatgaataagtctgctcagagaatctgtttgcc
aacgttcgacccagaagagttgattaccctaattgggaaactgatccagcaagataagtgcttagttcctgaaggaaaa
ggttactctttatatatcaggcctacattaatcggcactacggccggtttaggggtttccacgcctgatagagccttgc
tatatgtcatttgctgccctgtgggtccttattacaaaactggatttaaggcggtcagactggaagccactgattatgc
cacaagagcttggccaggaggctgtggtgacaagaaactaggtgcaaactacgccccctgcgtcctgccacaattgcaa
gctgcttcaaggggttaccaacaaaatttatggctatttggtccaaataacaacattactgaagtcggcaccatgaatg
cttttttcgtgtttaaagatagtaaaacgggcaagaaggaactggttactgctccactagacggtaccattttggaagg
tgttactagggattccattttaaatcttgctaaagaaagactcgaaccaagtgaatggaccattagtgaacgctacttc
actataggcgaagttactgagagatccaagaacggtgaactacttgaagcctttggttctggtactgctgcgattgttt
ctcccattaaggaaatcggctggaaaggcgaacaaattaatattccgttgttgcccggcgaacaaaccggtccattggc
caaagaagttgcacaatggattaatggaatccaatatggcgagactgagcatggcaattggtcaagggttgttactgat
ttgaactaataatactagagattaaagaggagaaatactagatgaattctagctatacacagagatatgcactgccgaa
gtgtatagcaatatcagattatcttttccatcggctcaaccagctgaacatacataccatatttggactctccggagaa
tttagcatgccgttgctggataaactatacaacattccgaacttacgatgggccggtaattctaatgagttaaatgctg
cctacgcagcagatggatactcacgactaaaaggcttgggatgtctcataacaacctttggtgtaggcgaattatcggc
aatcaatggcgtggccggatcttacgctgaacatgtaggaatacttcacatagtgggtatgccgccaacaagtgcacaa
acgaaacaactactactgcatcatactctgggcaatggtgatttcacggtatttcatagaatagccagtgatgtagcat
gctatacaacattgattattgactctgaattatgtgccgacgaagtcgataagtgcatcaaaaaggcttggatagaaca
gaggccagtatacatgggcatgcctgtcaaccaggtaaatctcccgattgaatcagcaaggcttaatacacctctggat
ttacaattgcataaaaacgacccagacgtagagaaagaagttatttctcgaatattgagttttatatacaaaagccaga
atccggcaatcatcgtagatgcatgtactagtcgacagaatttaatcgaggagactaaagagctttgtaataggcttaa
atttccagtttttgttacacctatgggtaagggtacagtaaacgaaacagacccgcaatttgggggcgtattcacgggc
tcgatatcagccccagaagtaagagaagtagttgattttgccgattttatcatcgtcattggttgcatgctctccgaat
tcagcacgtcaactttccacttccaatataaaactaagaattgtgcgctactatattctacatctgtgaaattgaaaaa
tgccacatatcctgacttgagcattaaattactactacagaaaatattagcaaatcttgatgaatctaaactgtcttac
caaccaagcgaacaacccagtatgatggttccaagaccttacccagcaggaaatgtcctcttgagacaagaatgggtct
ggaatgaaatatcccattggttccaaccaggtgacataatcataacagaaactggtgcttctgcatttggagttaacca
gaccagatttccggtaaatacactaggtatttcgcaagctctttggggatctgtcggatatacaatgggggcgtgtctt
ggggcagaatttgctgttcaagagataaacaaggataaattccccgcaactaaacatagagttattctgtttatgggtg
acggtgctttccaattgacagttcaagaattatccacaattgttaagtggggattgacaccttatatttttgtgatgaa
taaccaaggttactctgtggacaggtttttgcatcacaggtcagatgctagttattacgatatccaaccttggaactac
ttgggattattgcgagtatttggttgcacgaactacgaaacgaaaaaaattattactgttggagaattcagatccatga
tcagtgacccaaactttgcgaccaatgacaaaattcggatgatagagattatgctaccaccaagggatgttccacaggc
tctgcttgacaggtgggtggtagaaaaagaacagagcaaacaagtgcaagaggagaacgaaaattctagcgcagtaaat
acgccaactccagaattccaaccacttctaaaaaaaaatcaagttggatactga