SBOBS
type: ComponentDefinition
persistent identity: BBa J45600
display id: BBa_J45600
version: 1
was generated by: iGEM to SBOL conversion
description: Stationary phase banana odor biosynthetic system
created: 2006-10-30T12:00:00Z
modified: 2015-08-31T04:08:50Z
mutable provenance: This system is composed of devices <bbpart>J45120</bbpart> and <bbpart>J45320</bbpart>.
owned by: james
owned by: myers
mutable description: This system designed by the MIT 2006 iGEM team generates mint smell by biosynthesizing salicylic acid from chassis-produced metabolites (chorismate) and then converts salicylic acid into methyl salicylate (wintergreen scent).
discontinued: false
dominant: false
experience: experience/None
group u list: _84_
m user id: 0
owner id: 2
owning group id: 84
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: We recommend use of strain <bbpart>BBa_J45999</bbpart> as it does not produce indole. Indole is responsible for the foul smell of wt e.coli, and masks the wintergreen scent produced by J45600.
star: false
creator: MIT iGEM 2006
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_R0011
component: BBa_B0030
component: BBa_J45008
component: BBa_J45009
component: BBa_J45992
component: BBa_J45014
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_J45992
sequence annotation: BBa_B0030
sequence annotation: BBa_J45014
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_R0011
sequence annotation: BBa_J45008
sequence annotation: BBa_J45009
sequence: https://synbiohub.org/public/igem/BBa_J45600_sequence/1

BBa_R0011
type: Component
persistent identity: component1961077
display id: component1961077
version: 1
top level: SBOBS
definition: lacI+pL
access: public

BBa_B0030
type: Component
persistent identity: component1961090
display id: component1961090
version: 1
top level: SBOBS
definition: BBa_B0030
access: public

BBa_J45008
type: Component
persistent identity: component1961088
display id: component1961088
version: 1
top level: SBOBS
definition: BAT2
access: public

BBa_J45009
type: Component
persistent identity: component1961097
display id: component1961097
version: 1
top level: SBOBS
definition: THI3
access: public

BBa_J45992
type: Component
persistent identity: component1961062
display id: component1961062
version: 1
top level: SBOBS
definition: p(osmY)
access: public

BBa_J45014
type: Component
persistent identity: component1961070
display id: component1961070
version: 1
top level: SBOBS
definition: ATF1
access: public

BBa_B0030
type: Component
persistent identity: component1961064
display id: component1961064
version: 1
top level: SBOBS
definition: BBa_B0030
access: public

BBa_B0012
type: Component
persistent identity: component1961073
display id: component1961073
version: 1
top level: SBOBS
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1961071
display id: component1961071
version: 1
top level: SBOBS
definition: BBa_B0010
access: public

BBa_B0030
type: Component
persistent identity: component1961083
display id: component1961083
version: 1
top level: SBOBS
definition: BBa_B0030
access: public

BBa_J45992
type: SequenceAnnotation
persistent identity: annotation1961062
display id: annotation1961062
version: 1
top level: SBOBS
location: https://synbiohub.org/public/igem/BBa_J45600/annotation1961062/range1961062/1
component: BBa_J45992

https://synbiohub.org/public/igem/BBa_J45600/annotation1961062/range1961062/1
type: Range
persistent identity: range1961062
display id: range1961062
version: 1
top level: SBOBS
direction: direction/reverse
start: 1
end: 199
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1961064
display id: annotation1961064
version: 1
top level: SBOBS
location: https://synbiohub.org/public/igem/BBa_J45600/annotation1961064/range1961064/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_J45600/annotation1961064/range1961064/1
type: Range
persistent identity: range1961064
display id: range1961064
version: 1
top level: SBOBS
direction: direction/forward
start: 208
end: 222
orientation: inline

BBa_J45014
type: SequenceAnnotation
persistent identity: annotation1961070
display id: annotation1961070
version: 1
top level: SBOBS
location: https://synbiohub.org/public/igem/BBa_J45600/annotation1961070/range1961070/1
component: BBa_J45014

https://synbiohub.org/public/igem/BBa_J45600/annotation1961070/range1961070/1
type: Range
persistent identity: range1961070
display id: range1961070
version: 1
top level: SBOBS
direction: direction/reverse
start: 229
end: 1809
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1961071
display id: annotation1961071
version: 1
top level: SBOBS
location: https://synbiohub.org/public/igem/BBa_J45600/annotation1961071/range1961071/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J45600/annotation1961071/range1961071/1
type: Range
persistent identity: range1961071
display id: range1961071
version: 1
top level: SBOBS
direction: direction/forward
start: 1818
end: 1897
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1961073
display id: annotation1961073
version: 1
top level: SBOBS
location: https://synbiohub.org/public/igem/BBa_J45600/annotation1961073/range1961073/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J45600/annotation1961073/range1961073/1
type: Range
persistent identity: range1961073
display id: range1961073
version: 1
top level: SBOBS
direction: direction/forward
start: 1906
end: 1946
orientation: inline

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation1961077
display id: annotation1961077
version: 1
top level: SBOBS
location: https://synbiohub.org/public/igem/BBa_J45600/annotation1961077/range1961077/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_J45600/annotation1961077/range1961077/1
type: Range
persistent identity: range1961077
display id: range1961077
version: 1
top level: SBOBS
direction: direction/forward
start: 1955
end: 2008
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1961083
display id: annotation1961083
version: 1
top level: SBOBS
location: https://synbiohub.org/public/igem/BBa_J45600/annotation1961083/range1961083/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_J45600/annotation1961083/range1961083/1
type: Range
persistent identity: range1961083
display id: range1961083
version: 1
top level: SBOBS
direction: direction/forward
start: 2018
end: 2032
orientation: inline

BBa_J45008
type: SequenceAnnotation
persistent identity: annotation1961088
display id: annotation1961088
version: 1
top level: SBOBS
location: https://synbiohub.org/public/igem/BBa_J45600/annotation1961088/range1961088/1
component: BBa_J45008

https://synbiohub.org/public/igem/BBa_J45600/annotation1961088/range1961088/1
type: Range
persistent identity: range1961088
display id: range1961088
version: 1
top level: SBOBS
direction: direction/reverse
start: 2039
end: 3172
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1961090
display id: annotation1961090
version: 1
top level: SBOBS
location: https://synbiohub.org/public/igem/BBa_J45600/annotation1961090/range1961090/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_J45600/annotation1961090/range1961090/1
type: Range
persistent identity: range1961090
display id: range1961090
version: 1
top level: SBOBS
direction: direction/forward
start: 3181
end: 3195
orientation: inline

BBa_J45009
type: SequenceAnnotation
persistent identity: annotation1961097
display id: annotation1961097
version: 1
top level: SBOBS
location: https://synbiohub.org/public/igem/BBa_J45600/annotation1961097/range1961097/1
component: BBa_J45009

https://synbiohub.org/public/igem/BBa_J45600/annotation1961097/range1961097/1
type: Range
persistent identity: range1961097
display id: range1961097
version: 1
top level: SBOBS
direction: direction/reverse
start: 3202
end: 5031
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

BBa_B0030
type: ComponentDefinition
persistent identity: BBa B0030
display id: BBa_B0030
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.1 (strong) -- modified from R. Weiss
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _44_46_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: RBS-1\Strong
sequence annotation: BBa_B0030
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0030_sequence/1

RBS-1\Strong
type: SequenceAnnotation
persistent identity: annotation1701
display id: annotation1701
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
type: Range
persistent identity: range1701
display id: range1701
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation7025
display id: annotation7025
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
type: Range
persistent identity: range7025
display id: range7025
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1702
display id: annotation1702
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
type: Range
persistent identity: range1702
display id: range1702
version: 1
top level: BBa_B0030
direction: direction/forward
start: 8
end: 12
orientation: inline

lacI+pL
type: ComponentDefinition
persistent identity: BBa R0011
display id: BBa_R0011
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (lacI regulated, lambda pL hybrid)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: represillator of Elowitz and Leibler (2000)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The PLlac 0-1 promoter is a hybrid regulatory region consisting of the promoter P(L) of phage lambda with the cI binding sites replaced with lacO1. The hybrid design allows for strong promotion that can nevertheless be tightly repressed by LacI, the Lac inhibitor (i.e. repressor) (<bb_part>BBa_C0010</bb_part>) ([LUTZ97]). The activity of the promoter can be regulated over a >600-fold range by IPTG in E.Coli DH5-alpha-Z1 (same paper reference).
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: hybrid promoter design to create strong promoter that is, at the same time, highly repressible. note that the upstream operator installed in this hybrid is slightly different than the one in the original source (Lutz and Bujard, 1997). the most upstream operator region is slightly truncated in the represillator version, so that both operators in the hybrid are the same sequence. see references for details. also, the sequence has been truncated after the transcriptional start site. LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and increase transcription. This part is incompatible with environments containing lactose or lactose analogs.
star: true
creator: Neelaksh Varshney, Grace Kenney, Daniel Shen, Samantha Sutton
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0011
sequence annotation: lac O1
sequence annotation: -35
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0011_sequence/1

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation7064
display id: annotation7064
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation7064/range7064/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0011/annotation7064/range7064/1
type: Range
persistent identity: range7064
display id: range7064
version: 1
top level: lacI+pL
direction: direction/forward
start: 1
end: 54
orientation: inline

lac O1
type: SequenceAnnotation
persistent identity: annotation1999
display id: annotation1999
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation1999/range1999/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_R0011/annotation1999/range1999/1
type: Range
persistent identity: range1999
display id: range1999
version: 1
top level: lacI+pL
direction: direction/forward
start: 3
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2000
display id: annotation2000
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2000/range2000/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0011/annotation2000/range2000/1
type: Range
persistent identity: range2000
display id: range2000
version: 1
top level: lacI+pL
direction: direction/forward
start: 20
end: 25
orientation: inline

lac O1
type: SequenceAnnotation
persistent identity: annotation2001
display id: annotation2001
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2001/range2001/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_R0011/annotation2001/range2001/1
type: Range
persistent identity: range2001
display id: range2001
version: 1
top level: lacI+pL
direction: direction/forward
start: 26
end: 42
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2002
display id: annotation2002
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2002/range2002/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0011/annotation2002/range2002/1
type: Range
persistent identity: range2002
display id: range2002
version: 1
top level: lacI+pL
direction: direction/forward
start: 43
end: 48
orientation: inline

p(osmY)
type: ComponentDefinition
persistent identity: BBa J45992
display id: BBa_J45992
version: 1
was generated by: iGEM to SBOL conversion
description: Full-length stationary phase osmY promoter
created: 2006-07-10T11:00:00Z
modified: 2015-08-31T04:08:50Z
mutable provenance: This part was PCRed out of E. coli's genome.
owned by: james
owned by: myers
mutable description: osmY's promoter is active in stationary phase and under high osmotic pressure conditions.
discontinued: false
dominant: false
experience: experience/Works
group u list: _84_
m user id: 0
owner id: 642
owning group id: 84
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable notes: Truncated Forward Primer: 5'- GTT TCT TCG AAT TCG CGG CCG CTT CTA GGCT TAT GTT TTC GCT GAT ATC - 3' Total Length: 49 bp Annealing Length: 21 bp GC Content: 38.1% Melting Temperature: 49.5 degrees C hairpin deltaG: -2.66 kcal/mol self dimer deltaG: -99.97 kcal/mol Full Forward Primer: 5'- GTT TCT TCG AAT TCG CGG CCG CTT CTA GCTG GCA CAG GAA CGT TAT C - 3' Total Length: 47 bp Annealing Length: 19 bp GC Content: 52.6% Melting Temperature: 53.4 degrees C hairpin deltaG: -3.99 kcal/mol self dimer deltaG: -98.3 kcal/mol Reverse Primer: 5'-GTT TCT TCC TGC AGC GGC CGC TAC TAG TAT TGT TAA ATA TAG ATC ACA ATT TTG- 3' Total Length: 54 bp Annealing Length: 25 bp GC Content: 20.0% Melting Temperature: 46.4 degrees C hairpin deltaG: -2.86 kcal/mol self dimer deltaG: -99.44 kcal/mol heterodimer deltaG with Forward Primer: -99.44 kcal/mol heterodimer deltaG with Conserved Forward Primer: -99.97 kcal/mol
star: false
creator: Stephen Payne
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: -35
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_J45992_sequence/1

-35
type: SequenceAnnotation
persistent identity: annotation1883646
display id: annotation1883646
version: 1
top level: p(osmY)
location: https://synbiohub.org/public/igem/BBa_J45992/annotation1883646/range1883646/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_J45992/annotation1883646/range1883646/1
type: Range
persistent identity: range1883646
display id: range1883646
version: 1
top level: p(osmY)
direction: direction/forward
start: 164
end: 169
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1883645
display id: annotation1883645
version: 1
top level: p(osmY)
location: https://synbiohub.org/public/igem/BBa_J45992/annotation1883645/range1883645/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_J45992/annotation1883645/range1883645/1
type: Range
persistent identity: range1883645
display id: range1883645
version: 1
top level: p(osmY)
direction: direction/forward
start: 188
end: 193
orientation: inline

BAT2
type: ComponentDefinition
persistent identity: BBa J45008
display id: BBa_J45008
version: 1
was generated by: iGEM to SBOL conversion
description: branched-chain amino acid transaminase (BAT2); converts leucine to alpha-ketoisocaproate
created: 2006-06-06T11:00:00Z
modified: 2015-08-31T04:08:49Z
mutable provenance: dkfkas
owned by: james
owned by: myers
mutable description: fd;lsfas
discontinued: false
dominant: false
experience: experience/Fails
group u list: _84_
m user id: 0
owner id: 642
owning group id: 84
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: dakfdas;
star: false
creator: Veena Venkatachalam
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: start codon
sequence annotation: BAT2
sequence annotation: a->g to eliminate SpeI
sequence annotation: double TAA stop codon
sequence: https://synbiohub.org/public/igem/BBa_J45008_sequence/1

start codon
type: SequenceAnnotation
persistent identity: annotation1883624
display id: annotation1883624
version: 1
top level: BAT2
location: https://synbiohub.org/public/igem/BBa_J45008/annotation1883624/range1883624/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_J45008/annotation1883624/range1883624/1
type: Range
persistent identity: range1883624
display id: range1883624
version: 1
top level: BAT2
direction: direction/forward
start: 1
end: 3
orientation: inline

BAT2
type: SequenceAnnotation
persistent identity: annotation1961299
display id: annotation1961299
version: 1
top level: BAT2
location: https://synbiohub.org/public/igem/BBa_J45008/annotation1961299/range1961299/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_J45008/annotation1961299/range1961299/1
type: Range
persistent identity: range1961299
display id: range1961299
version: 1
top level: BAT2
direction: direction/forward
start: 1
end: 1134
orientation: inline

a->g to eliminate SpeI
type: SequenceAnnotation
persistent identity: annotation1894553
display id: annotation1894553
version: 1
top level: BAT2
location: https://synbiohub.org/public/igem/BBa_J45008/annotation1894553/range1894553/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_J45008/annotation1894553/range1894553/1
type: Range
persistent identity: range1894553
display id: range1894553
version: 1
top level: BAT2
direction: direction/forward
start: 771
end: 771
orientation: inline

double TAA stop codon
type: SequenceAnnotation
persistent identity: annotation1883625
display id: annotation1883625
version: 1
top level: BAT2
location: https://synbiohub.org/public/igem/BBa_J45008/annotation1883625/range1883625/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_J45008/annotation1883625/range1883625/1
type: Range
persistent identity: range1883625
display id: range1883625
version: 1
top level: BAT2
direction: direction/forward
start: 1129
end: 1134
orientation: inline

THI3
type: ComponentDefinition
persistent identity: BBa J45009
display id: BBa_J45009
version: 1
was generated by: iGEM to SBOL conversion
description: ketoisocaproate decarboxylase (THI3); converts alpha-ketoisocaproate to 3-methylbutanal
created: 2006-06-06T11:00:00Z
modified: 2015-08-31T04:08:49Z
mutable provenance: dfd
owned by: james
owned by: myers
mutable description: dfsd
discontinued: false
dominant: false
experience: experience/Fails
group u list: _84_
m user id: 0
owner id: 642
owning group id: 84
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable notes: dfd
star: false
creator: Veena Venkatachalam
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: start codon
sequence annotation: THI3
sequence annotation: t->c to eliminate EcoRI, to do
sequence annotation: t->c to eliminate SpeI, to do
sequence annotation: a->g to eliminate EcoRI, to do
sequence annotation: stop codon
sequence: https://synbiohub.org/public/igem/BBa_J45009_sequence/1

start codon
type: SequenceAnnotation
persistent identity: annotation1961303
display id: annotation1961303
version: 1
top level: THI3
location: https://synbiohub.org/public/igem/BBa_J45009/annotation1961303/range1961303/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_J45009/annotation1961303/range1961303/1
type: Range
persistent identity: range1961303
display id: range1961303
version: 1
top level: THI3
direction: direction/forward
start: 1
end: 3
orientation: inline

THI3
type: SequenceAnnotation
persistent identity: annotation1961298
display id: annotation1961298
version: 1
top level: THI3
location: https://synbiohub.org/public/igem/BBa_J45009/annotation1961298/range1961298/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_J45009/annotation1961298/range1961298/1
type: Range
persistent identity: range1961298
display id: range1961298
version: 1
top level: THI3
direction: direction/forward
start: 1
end: 1830
orientation: inline

t->c to eliminate EcoRI, to do
type: SequenceAnnotation
persistent identity: annotation1891628
display id: annotation1891628
version: 1
top level: THI3
location: https://synbiohub.org/public/igem/BBa_J45009/annotation1891628/range1891628/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_J45009/annotation1891628/range1891628/1
type: Range
persistent identity: range1891628
display id: range1891628
version: 1
top level: THI3
direction: direction/forward
start: 6
end: 6
orientation: inline

t->c to eliminate SpeI, to do
type: SequenceAnnotation
persistent identity: annotation1891632
display id: annotation1891632
version: 1
top level: THI3
location: https://synbiohub.org/public/igem/BBa_J45009/annotation1891632/range1891632/1
role: SO:1000008
role: feature/s mutation

https://synbiohub.org/public/igem/BBa_J45009/annotation1891632/range1891632/1
type: Range
persistent identity: range1891632
display id: range1891632
version: 1
top level: THI3
direction: direction/forward
start: 699
end: 699
orientation: inline

a->g to eliminate EcoRI, to do
type: SequenceAnnotation
persistent identity: annotation1891629
display id: annotation1891629
version: 1
top level: THI3
location: https://synbiohub.org/public/igem/BBa_J45009/annotation1891629/range1891629/1
role: SO:1000008
role: feature/s mutation

https://synbiohub.org/public/igem/BBa_J45009/annotation1891629/range1891629/1
type: Range
persistent identity: range1891629
display id: range1891629
version: 1
top level: THI3
direction: direction/forward
start: 906
end: 906
orientation: inline

a->g to eliminate EcoRI, to do
type: SequenceAnnotation
persistent identity: annotation1891630
display id: annotation1891630
version: 1
top level: THI3
location: https://synbiohub.org/public/igem/BBa_J45009/annotation1891630/range1891630/1
role: SO:1000008
role: feature/s mutation

https://synbiohub.org/public/igem/BBa_J45009/annotation1891630/range1891630/1
type: Range
persistent identity: range1891630
display id: range1891630
version: 1
top level: THI3
direction: direction/forward
start: 1605
end: 1605
orientation: inline

a->g to eliminate EcoRI, to do
type: SequenceAnnotation
persistent identity: annotation1891631
display id: annotation1891631
version: 1
top level: THI3
location: https://synbiohub.org/public/igem/BBa_J45009/annotation1891631/range1891631/1
role: SO:1000008
role: feature/s mutation

https://synbiohub.org/public/igem/BBa_J45009/annotation1891631/range1891631/1
type: Range
persistent identity: range1891631
display id: range1891631
version: 1
top level: THI3
direction: direction/forward
start: 1791
end: 1791
orientation: inline

stop codon
type: SequenceAnnotation
persistent identity: annotation1961304
display id: annotation1961304
version: 1
top level: THI3
location: https://synbiohub.org/public/igem/BBa_J45009/annotation1961304/range1961304/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_J45009/annotation1961304/range1961304/1
type: Range
persistent identity: range1961304
display id: range1961304
version: 1
top level: THI3
direction: direction/forward
start: 1828
end: 1830
orientation: inline

ATF1
type: ComponentDefinition
persistent identity: BBa J45014
display id: BBa_J45014
version: 1
was generated by: iGEM to SBOL conversion
description: alcohol acetyltransferase I; converts isoamyl alcohol to isoamyl acetate (banana odor)
created: 2006-07-30T11:00:00Z
modified: 2015-08-31T04:08:49Z
mutable provenance: This part comes from BBa_45J006.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: This part is the ATF sequence (BBa_45J006) with a single point mutation at base pair 1176 (T -> C).
discontinued: false
dominant: false
experience: experience/Works
group u list: _84_
m user id: 0
owner id: 977
owning group id: 84
sample status: In stock
status: status/Available
bookmark: true
mutable notes: It has a single point mutation at base pair 1176 (T -> C).
star: true
creator: Andr?? Green II
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: ATF1
sequence annotation: a->g to eliminate EcoRI site
sequence annotation: t -> c
sequence annotation: double TAA stop codon
sequence: https://synbiohub.org/public/igem/BBa_J45014_sequence/1

ATF1
type: SequenceAnnotation
persistent identity: annotation1961300
display id: annotation1961300
version: 1
top level: ATF1
location: https://synbiohub.org/public/igem/BBa_J45014/annotation1961300/range1961300/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_J45014/annotation1961300/range1961300/1
type: Range
persistent identity: range1961300
display id: range1961300
version: 1
top level: ATF1
direction: direction/forward
start: 1
end: 1581
orientation: inline

a->g to eliminate EcoRI site
type: SequenceAnnotation
persistent identity: annotation1961305
display id: annotation1961305
version: 1
top level: ATF1
location: https://synbiohub.org/public/igem/BBa_J45014/annotation1961305/range1961305/1
role: SO:1000008
role: feature/s mutation

https://synbiohub.org/public/igem/BBa_J45014/annotation1961305/range1961305/1
type: Range
persistent identity: range1961305
display id: range1961305
version: 1
top level: ATF1
direction: direction/forward
start: 414
end: 414
orientation: inline

t -> c
type: SequenceAnnotation
persistent identity: annotation1961301
display id: annotation1961301
version: 1
top level: ATF1
location: https://synbiohub.org/public/igem/BBa_J45014/annotation1961301/range1961301/1
role: feature/mutation
role: SO:0001059

https://synbiohub.org/public/igem/BBa_J45014/annotation1961301/range1961301/1
type: Range
persistent identity: range1961301
display id: range1961301
version: 1
top level: ATF1
direction: direction/forward
start: 1148
end: 1148
orientation: inline

double TAA stop codon
type: SequenceAnnotation
persistent identity: annotation1961302
display id: annotation1961302
version: 1
top level: ATF1
location: https://synbiohub.org/public/igem/BBa_J45014/annotation1961302/range1961302/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_J45014/annotation1961302/range1961302/1
type: Range
persistent identity: range1961302
display id: range1961302
version: 1
top level: ATF1
direction: direction/forward
start: 1576
end: 1581
orientation: inline

https://synbiohub.org/public/igem/BBa_J45600_sequence/1
type: Sequence
persistent identity: BBa J45600 sequence
display id: BBa_J45600_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctggcacaggaacgttatccggacgttcagttccaccagacccgcgagcattaattcttgcctccagggcgcggtagtggcgccctgtcaatttcccttccttattagccgcttacggaatgttcttaaaacattcacttttgcttatgttttcgctgatatcccgagcggtttcaaaattgtgatctatatttaacaatactagagattaaagaggagaaatactagatgaatgaaatcgatgagaaaaatcaggcccccgtgcaacaagaatgcctgaaagagatgattcagaatgggcatgctcggcgtatgggatctgttgaagatctgtatgttgctctcaacagacaaaacttatatcgaaacttctgcacatatggagaattgagtgattactgtactagggatcagctcacattagctttgagggaaatctgcctgaaaaatccaactcttttacatattgttctaccaacaagatggccaaatcatgaaaattattatcgcagttccgaatactattcacggccacatccagtgcatgattatatttcagtattacaagaattgaaactgagtggtgtggttctcaatgaacaacctgagtacagtgcagtaatgaagcaaatattagaagagttcaaaaatagtaagggttcctatactgcaaaaatttttaaacttactaccactttgactattccttactttggaccaacaggaccgagttggcggctaatttgtcttccagaagagcacacagaaaagtggaaaaaatttatctttgtatctaatcattgcatgtctgatggtcggtcttcgatccacttttttcatgatttaagagacgaattaaataatattaaaactccaccaaaaaaattagattacattttcaagtacgaggaggattaccaattattgaggaaacttccagaaccgatcgaaaaggtgatagactttagaccaccgtacttgtttattccgaagtcacttctttcgggtttcatctacaatcatttgagattttcttcaaaaggtgtctgtatgagaatggatgatgtggaaaaaaccgatgatgttgtcaccgagatcatcaatatttcaccaacagaatttcaagcgattaaagcaaatattaaatcaaatatccaaggtaagtgtactatcactccgtttttacatgtttgttggtttgtatctcttcataaatggggtaaatttttcaaaccattgaacttcgaatggcttacggatatttttatccccgcagattgccgctcacaactaccagatgatgatgaaatgagacagatgtacagatatggcgctaacgttggatttattgacttcaccccctggataagcgaatctgacatgaatgataacaaagaaaatttttggccacttattgagcactaccatgaagtaatttcggaagctttaagaaataaaaagcatctccatggcttagggttcaatatacaaggcttcgttcaaaaatatgtgaacattgacaaggtaatgtgcgatcgtgccatcgggaaaagacgcggaggtacattgttaagcaatgtaggtctgtttaatcagttagaggagcccgatgccaaatattctatatgcgatttggcatttggccaatttcaaggatcctggcaccaagcattttccttgggtgtttgttcgactaatgtaaaggggatgaatattgttgttgcttcaacaaagaatgttgttggtagtcaagaatctctcgaagagctttgctccatttacaaagctctccttttaggcccttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagaattgtgagcggataacaattgacattgtgagcggataacaagata…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0030_sequence/1
type: Sequence
persistent identity: BBa B0030 sequence
display id: BBa_B0030_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0011_sequence/1
type: Sequence
persistent identity: BBa R0011 sequence
display id: BBa_R0011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J45992_sequence/1
type: Sequence
persistent identity: BBa J45992 sequence
display id: BBa_J45992_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctggcacaggaacgttatccggacgttcagttccaccagacccgcgagcattaattcttgcctccagggcgcggtagtggcgccctgtcaatttcccttccttattagccgcttacggaatgttcttaaaacattcacttttgcttatgttttcgctgatatcccgagcggtttcaaaattgtgatctatatttaacaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J45008_sequence/1
type: Sequence
persistent identity: BBa J45008 sequence
display id: BBa_J45008_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaccttggcacccctagacgcctccaaagttaagataactaccacacaacatgcatctaagccaaaaccgaacagtgagttagtgtttggcaagagcttcacggaccacatgttaactgcggaatggacagctgaaaaagggtggggtaccccagagattaaaccttatcaaaatctgtctttagacccttccgcggtggttttccattatgcttttgagctattcgaagggatgaaggcttacagaacggtggacaacaaaattacaatgtttcgtccagatatgaatatgaagcgcatgaataagtctgctcagagaatctgtttgccaacgttcgacccagaagagttgattaccctaattgggaaactgatccagcaagataagtgcttagttcctgaaggaaaaggttactctttatatatcaggcctacattaatcggcactacggccggtttaggggtttccacgcctgatagagccttgctatatgtcatttgctgccctgtgggtccttattacaaaactggatttaaggcggtcagactggaagccactgattatgccacaagagcttggccaggaggctgtggtgacaagaaactaggtgcaaactacgccccctgcgtcctgccacaattgcaagctgcttcaaggggttaccaacaaaatttatggctatttggtccaaataacaacattactgaagtcggcaccatgaatgcttttttcgtgtttaaagatagtaaaacgggcaagaaggaactggttactgctccactagacggtaccattttggaaggtgttactagggattccattttaaatcttgctaaagaaagactcgaaccaagtgaatggaccattagtgaacgctacttcactataggcgaagttactgagagatccaagaacggtgaactacttgaagcctttggttctggtactgctgcgattgtttctcccattaaggaaatcggctggaaaggcgaacaaattaatattccgttgttgcccggcgaacaaaccggtccattggccaaagaagttgcacaatggattaatggaatccaatatggcgagactgagcatggcaattggtcaagggttgttactgatttgaactaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J45009_sequence/1
type: Sequence
persistent identity: BBa J45009 sequence
display id: BBa_J45009_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaattctagctatacacagagatatgcactgccgaagtgtatagcaatatcagattatcttttccatcggctcaaccagctgaacatacataccatatttggactctccggagaatttagcatgccgttgctggataaactatacaacattccgaacttacgatgggccggtaattctaatgagttaaatgctgcctacgcagcagatggatactcacgactaaaaggcttgggatgtctcataacaacctttggtgtaggcgaattatcggcaatcaatggcgtggccggatcttacgctgaacatgtaggaatacttcacatagtgggtatgccgccaacaagtgcacaaacgaaacaactactactgcatcatactctgggcaatggtgatttcacggtatttcatagaatagccagtgatgtagcatgctatacaacattgattattgactctgaattatgtgccgacgaagtcgataagtgcatcaaaaaggcttggatagaacagaggccagtatacatgggcatgcctgtcaaccaggtaaatctcccgattgaatcagcaaggcttaatacacctctggatttacaattgcataaaaacgacccagacgtagagaaagaagttatttctcgaatattgagttttatatacaaaagccagaatccggcaatcatcgtagatgcatgtactagtcgacagaatttaatcgaggagactaaagagctttgtaataggcttaaatttccagtttttgttacacctatgggtaagggtacagtaaacgaaacagacccgcaatttgggggcgtattcacgggctcgatatcagccccagaagtaagagaagtagttgattttgccgattttatcatcgtcattggttgcatgctctccgaattcagcacgtcaactttccacttccaatataaaactaagaattgtgcgctactatattctacatctgtgaaattgaaaaatgccacatatcctgacttgagcattaaattactactacagaaaatattagcaaatcttgatgaatctaaactgtcttaccaaccaagcgaacaacccagtatgatggttccaagaccttacccagcaggaaatgtcctcttgagacaagaatgggtctggaatgaaatatcccattggttccaaccaggtgacataatcataacagaaactggtgcttctgcatttggagttaaccagaccagatttccggtaaatacactaggtatttcgcaagctctttggggatctgtcggatatacaatgggggcgtgtcttggggcagaatttgctgttcaagagataaacaaggataaattccccgcaactaaacatagagttattctgtttatgggtgacggtgctttccaattgacagttcaagaattatccacaattgttaagtggggattgacaccttatatttttgtgatgaataaccaaggttactctgtggacaggtttttgcatcacaggtcagatgctagttattacgatatccaaccttggaactacttgggattattgcgagtatttggttgcacgaactacgaaacgaaaaaaattattactgttggagaattcagatccatgatcagtgacccaaactttgcgaccaatgacaaaattcggatgatagagattatgctaccaccaagggatgttccacaggctctgcttgacaggtgggtggtagaaaaagaacagagcaaacaagtgcaagaggagaacgaaaattctagcgcagtaaatacgccaactccagaattccaaccacttctaaaaaaaaatcaagttggatactga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J45014_sequence/1
type: Sequence
persistent identity: BBa J45014 sequence
display id: BBa_J45014_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaatgaaatcgatgagaaaaatcaggcccccgtgcaacaagaatgcctgaaagagatgattcagaatgggcatgctcggcgtatgggatctgttgaagatctgtatgttgctctcaacagacaaaacttatatcgaaacttctgcacatatggagaattgagtgattactgtactagggatcagctcacattagctttgagggaaatctgcctgaaaaatccaactcttttacatattgttctaccaacaagatggccaaatcatgaaaattattatcgcagttccgaatactattcacggccacatccagtgcatgattatatttcagtattacaagaattgaaactgagtggtgtggttctcaatgaacaacctgagtacagtgcagtaatgaagcaaatattagaagagttcaaaaatagtaagggttcctatactgcaaaaatttttaaacttactaccactttgactattccttactttggaccaacaggaccgagttggcggctaatttgtcttccagaagagcacacagaaaagtggaaaaaatttatctttgtatctaatcattgcatgtctgatggtcggtcttcgatccacttttttcatgatttaagagacgaattaaataatattaaaactccaccaaaaaaattagattacattttcaagtacgaggaggattaccaattattgaggaaacttccagaaccgatcgaaaaggtgatagactttagaccaccgtacttgtttattccgaagtcacttctttcgggtttcatctacaatcatttgagattttcttcaaaaggtgtctgtatgagaatggatgatgtggaaaaaaccgatgatgttgtcaccgagatcatcaatatttcaccaacagaatttcaagcgattaaagcaaatattaaatcaaatatccaaggtaagtgtactatcactccgtttttacatgtttgttggtttgtatctcttcataaatggggtaaatttttcaaaccattgaacttcgaatggcttacggatatttttatccccgcagattgccgctcacaactaccagatgatgatgaaatgagacagatgtacagatatggcgctaacgttggatttattgacttcaccccctggataagcgaatctgacatgaatgataacaaagaaaatttttggccacttattgagcactaccatgaagtaatttcggaagctttaagaaataaaaagcatctccatggcttagggttcaatatacaaggcttcgttcaaaaatatgtgaacattgacaaggtaatgtgcgatcgtgccatcgggaaaagacgcggaggtacattgttaagcaatgtaggtctgtttaatcagttagaggagcccgatgccaaatattctatatgcgatttggcatttggccaatttcaaggatcctggcaccaagcattttccttgggtgtttgttcgactaatgtaaaggggatgaatattgttgttgcttcaacaaagaatgttgttggtagtcaagaatctctcgaagagctttgctccatttacaaagctctccttttaggcccttaataa
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z