##gff-version 3
##sequence-region BBa_J45700 1 3255
BBa_J45700	.	terminator	1252	1292	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_J45700	.	engineered_region	1252	1292	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J45700	.	polyA_site	1279	1292	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J45700	.	stem_loop	1259	1278	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J45700	.	stop_codon	1285	1285	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J45700	.	CDS	1383	3118	.	+	0	ID=BBa_J45017;Name=BBa_J45017
BBa_J45700	.	start_codon	1685	1687	.	+	0	ID=annotation1897454;Name=pchA start codon;Parent=BBa_J45017
BBa_J45700	.	stop_codon	3113	3118	.	+	0	ID=annotation1897457;Name=pchA double TAA stop codon;Parent=BBa_J45017
BBa_J45700	.	CDS	1685	3118	.	+	0	ID=annotation1961297;Name=PchA;Parent=BBa_J45017
BBa_J45700	.	stop_codon	1686	1688	.	+	0	ID=annotation1897456;Name=pchB stop codon;Parent=BBa_J45017
BBa_J45700	.	CDS	1383	1688	.	+	0	ID=annotation1961296;Name=PchB;Parent=BBa_J45017
BBa_J45700	.	start_codon	1383	1385	.	+	0	ID=annotation1897455;Name=pchB start codon;Parent=BBa_J45017
BBa_J45700	.	point_mutation	1822	1822	.	+	0	ID=annotation1897453;Name=g -> a to eliminate PstI site;Parent=BBa_J45017
BBa_J45700	.	ribosome_entry_site	63	75	.	+	0	ID=BBa_B0032;Name=BBa_B0032
BBa_J45700	.	engineered_region	63	75	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_J45700	.	ribosome_entry_site	63	75	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_J45700	.	ribosome_entry_site	69	72	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_J45700	.	terminator	3127	3206	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_J45700	.	engineered_region	3127	3206	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J45700	.	stem_loop	3138	3181	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J45700	.	terminator	1164	1243	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_J45700	.	engineered_region	1164	1243	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J45700	.	stem_loop	1175	1218	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J45700	.	ribosome_entry_site	1364	1376	.	+	0	ID=BBa_B0032;Name=BBa_B0032
BBa_J45700	.	engineered_region	1364	1376	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_J45700	.	ribosome_entry_site	1364	1376	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_J45700	.	ribosome_entry_site	1370	1373	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_J45700	.	terminator	3215	3255	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_J45700	.	engineered_region	3215	3255	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J45700	.	polyA_site	3242	3255	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J45700	.	stem_loop	3222	3241	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J45700	.	stop_codon	3248	3248	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J45700	.	promoter	1301	1354	.	+	0	ID=BBa_R0011;Name=BBa_R0011
BBa_J45700	.	promoter	1343	1348	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_J45700	.	engineered_region	1301	1354	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_J45700	.	operator	1326	1342	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_J45700	.	operator	1303	1319	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_J45700	.	promoter	1320	1325	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_J45700	.	CDS	82	1155	.	+	0	ID=BBa_J45004;Name=BBa_J45004
BBa_J45700	.	CDS	82	1155	.	+	0	ID=annotation1880517;Name=BMST1;Parent=BBa_J45004
BBa_J45700	.	stop_codon	1153	1155	.	+	0	ID=annotation1880520;Name=stop codon;Parent=BBa_J45004
BBa_J45700	.	start_codon	82	84	.	+	0	ID=annotation1880519;Name=start codon;Parent=BBa_J45004
BBa_J45700	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_J45700	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_J45700	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_J45700	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_J45700	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_J45700	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
>BBa_J45700
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagtcacacaggaaagtact
agatggaagttgttgaagttcttcacatgaatggaggaaatggagacagtagctatgcaaacaattctttggttcagca
aaaggtgattctcatgacaaagccaataactgagcaagccatgattgatctctacagcagcctctttccagaaacctta
tgcattgcagatttgggttgttctttgggagctaacactttcttggtggtctcacagcttgttaaaatagtagaaaaag
aacgaaaaaagcatggttttaagtctccagagttttattttcacttcaatgatcttcctggcaatgattttaatacact
ttttcagtcactgggggcatttcaagaagatttgagaaagcatataggggaaagctttggtccatgttttttcagtgga
gtgcctggttcattttatactagacttttcccttccaaaagtttacattttgtttactcctcctacagtctcatgtggc
tatctcaggtgcctaatgggattgaaaataacaagggaaacatttacatggcaagaacaagccctctaagtgttattaa
agcatactacaagcaatatgaaatagatttttcaaattttctcaagtaccgttcagaggaattgatgaaaggtggaaag
atggtgttaacactcctaggtagagaaagtgaggatcctactagcaaagaatgctgttacatttgggagcttctagcca
tggccctcaataagttggttgaagagggattgataaaagaagagaaagtagatgcattcaatattcctcaatacacacc
atcaccagcagaagtaaagtacatagttgagaaggaaggatcattcaccattaatcgcttggaaacatcaagagttcat
tggaatgcttctaataatgagaagaatggtggttacaatgtgtcaaggtgcatgagagctgtggctgagcctttgcttg
tcagccactttgacaaggaattgatggatttagtgttccacaagtacgaagagattgtttctgattgcatgtccaaaga
gaatactgagtttataaatgtcatcatctccttgaccaaaataaattaatactagagccaggcatcaaataaaacgaaa
ggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctca
ccttcgggtgggcctttctgcgtttatatactagagaattgtgagcggataacaattgacattgtgagcggataacaag
atactgagcacatactagagtcacacaggaaagtactagatgaaaactcccgaagactgcaccggcctggcggacatcc
gcgaggccatcgaccggatcgacctggatatcgtccaggccctcggccgccgcatggactacgtcaaggcggcgtcgcg
cttcaaggccagcgaggcggcgattccggcgcccgagcgggtcgccgcgatgctccccgagcgcgcccgctgggccgag
gaaaacggactcgacgcgcccttcgtcgagggactgttcgcgcagatcatccactggtacatcgccgagcagatcaagt
actggcgccagacacggggtgccgcatgagccggctggcgcccctgagccagtgcctgcacgccttgcgcggcaccttc
gagcgcgccatcggccaggcgcaggcgctcgatcgtccggtgctggtggcggcatcgttcgagatcgacccattggacc
cgctacaggtattcggtgcctgggacgaccggcaaacgccctgcctgtactgggaacagcccgagctggcgttcttcgc
ctggggctgcgccctggagctgcaaggccacggcgaacagcgcttcgcccggatcgaggaaaactggcaattgctctgc
gccgacgccgtggtcgagggcccgctggcgccgcgcctgtgcggcggattccgcttcgatccgcgcggcccgcgcgagg
aacactggcaagccttcgccgatgccagcctgatgctcgccggcatcaccgtgctgcgcgagggcgaacgctaccgggt
actctgccaacacctggccaagcccggcgaagatgccctggccctggccgcctaccactgctcggcgctactgcgcctg
aggcagccggccagacgccggccctcggggccgaccgctggcgcgcagggcgacgcttcggcgcaggagcgcaggcaat
gggaagccaaggtgagcgacgcggtaagcagtgtccgccagggacgcttcggcaaggtcgtgctggcccgcacccaggc
ccggcctctcggcgacatcgagccgtggcaggtcatcgaacacctgcgtctgcaacatgccgacgcccagctgttcgcc
tgtcgccgcggcaacgcctgcttcctcggcgcctccccggaacgcctggtccgcattcgcgccggcgaggcactcaccc
atgccctggccgggaccatcgcccgcggcggcgatgcccaggaagatgcgcggctcggacaggccctgctggacagcgc
caaggacaggcacgaacaccagttggtggtggaggcgatccgtacggccctggaacccttcagcgaggtgctggaaatc
cccgatgcgcccggcctgaaacgactggcgcgagtccagcacctgaacacgccgatccgcgcccgcctcgctgacgcag
gcggcatcctgcggctgctacaagcgctgcatccgacccccgcggtgggcggctacccacgcagcgcggcgctggacta
catccgccagcacgaagggatggaccgcggctggtacgccgcgccgctgggctggctcgacggcgaaggcaacggcgat
ttcctggtggcgctgcgctcggccctgctcacgccgggccggggctacctgttcgccggctgcggtctggtaggcgatt
cggaaccggcccacgagtatcgcgaaacctgccttaagctcagtgccatgcgggaagctctatccgccataggcggcct
ggacgaagtgcccttgcagcgcggcgtcgcctaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaa
agactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtggg
cctttctgcgtttata