LOCUS BBa_J45800 4168 bp DNA linear 22-Dec-2024 DEFINITION Exponential phase wintergreen odor biosynthetic system ACCESSION BBa_J45800 VERSION BBa_J45800.1 FEATURES Location/Qualifiers misc_feature 3..19 /label=lac O1 promoter 20..25 /label=-35 misc_feature 26..42 /label=lac O1 promoter 43..48 /label=-10 misc_feature 1..54 /label=BBa_R0011 promoter 1..54 /label=BBa_R0011 RBS 64..76 /label=RBS-3\Weak RBS 70..73 /label=RBS misc_feature 64..76 /label=BBa_B0032 RBS 64..76 /label=BBa_B0032 misc_feature 522..522 /label=g -> a to eliminate PstI site misc_feature 385..387 /label=pchA start codon misc_feature 83..85 /label=pchB start codon misc_feature 386..388 /label=pchB stop codon misc_feature 1813..1818 /label=pchA double TAA stop codon CDS 83..388 /label=PchB CDS 385..1818 /label=PchA CDS 83..1818 /label=BBa_J45017 stem_loop 1838..1881 /label=stem_loop misc_feature 1827..1906 /label=BBa_B0010 terminator 1827..1906 /label=BBa_B0010 stem_loop 1922..1941 /label=T7 TE misc_feature 1948..1948 /label=stop polyA_site 1942..1955 /label=polya misc_feature 1915..1955 /label=BBa_B0012 terminator 1915..1955 /label=BBa_B0012 misc_feature 1..1955 /label=BBa_J45320 promoter 2009..2014 /label=-10 promoter 1985..1990 /label=-35 promoter 1964..2020 /label=BBa_J45993 RBS 2029..2040 /label=BBa_B0064 CDS 2050..2666 /label=tetR misc_feature 2667..2700 /label=SsrA misc_feature 2707..2731 /label=Help:Barcodes CDS 2047..2731 /label=BBa_C0040 stem_loop 2751..2794 /label=stem_loop misc_feature 2740..2819 /label=BBa_B0010 terminator 2740..2819 /label=BBa_B0010 stem_loop 2835..2854 /label=T7 TE misc_feature 2861..2861 /label=stop polyA_site 2855..2868 /label=polya misc_feature 2828..2868 /label=BBa_B0012 terminator 2828..2868 /label=BBa_B0012 terminator 2740..2868 /label=BBa_B0015 misc_feature 2877..2895 /label=TetR 1 misc_feature 2877..2930 /label=BBa_R0040 promoter 2896..2901 /label=-35 misc_feature 2902..2920 /label=TetR 2 promoter 2919..2924 /label=-10 promoter 2877..2930 /label=BBa_R0040 misc_feature 2029..2930 /label=BBa_Q04401 RBS 2939..2951 /label=RBS-3\Weak RBS 2945..2948 /label=RBS misc_feature 2939..2951 /label=BBa_B0032 RBS 2939..2951 /label=BBa_B0032 CDS 2958..4031 /label=BMST1 misc_feature 2958..2960 /label=start codon misc_feature 4029..4031 /label=stop codon CDS 2958..4031 /label=BBa_J45004 stem_loop 4051..4094 /label=stem_loop misc_feature 4040..4119 /label=BBa_B0010 terminator 4040..4119 /label=BBa_B0010 stem_loop 4135..4154 /label=T7 TE misc_feature 4161..4161 /label=stop polyA_site 4155..4168 /label=polya misc_feature 4128..4168 /label=BBa_B0012 terminator 4128..4168 /label=BBa_B0012 misc_feature 1964..4168 /label=BBa_J45180 ORIGIN 1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta 61 gagtcacaca ggaaagtact agatgaaaac tcccgaagac tgcaccggcc tggcggacat 121 ccgcgaggcc atcgaccgga tcgacctgga tatcgtccag gccctcggcc gccgcatgga 181 ctacgtcaag gcggcgtcgc gcttcaaggc cagcgaggcg gcgattccgg cgcccgagcg 241 ggtcgccgcg atgctccccg agcgcgcccg ctgggccgag gaaaacggac tcgacgcgcc 301 cttcgtcgag ggactgttcg cgcagatcat ccactggtac atcgccgagc agatcaagta 361 ctggcgccag acacggggtg ccgcatgagc cggctggcgc ccctgagcca gtgcctgcac 421 gccttgcgcg gcaccttcga gcgcgccatc ggccaggcgc aggcgctcga tcgtccggtg 481 ctggtggcgg catcgttcga gatcgaccca ttggacccgc tacaggtatt cggtgcctgg 541 gacgaccggc aaacgccctg cctgtactgg gaacagcccg agctggcgtt cttcgcctgg 601 ggctgcgccc tggagctgca aggccacggc gaacagcgct tcgcccggat cgaggaaaac 661 tggcaattgc tctgcgccga cgccgtggtc gagggcccgc tggcgccgcg cctgtgcggc 721 ggattccgct tcgatccgcg cggcccgcgc gaggaacact ggcaagcctt cgccgatgcc 781 agcctgatgc tcgccggcat caccgtgctg cgcgagggcg aacgctaccg ggtactctgc 841 caacacctgg ccaagcccgg cgaagatgcc ctggccctgg ccgcctacca ctgctcggcg 901 ctactgcgcc tgaggcagcc ggccagacgc cggccctcgg ggccgaccgc tggcgcgcag 961 ggcgacgctt cggcgcagga gcgcaggcaa tgggaagcca aggtgagcga cgcggtaagc 1021 agtgtccgcc agggacgctt cggcaaggtc gtgctggccc gcacccaggc ccggcctctc 1081 ggcgacatcg agccgtggca ggtcatcgaa cacctgcgtc tgcaacatgc cgacgcccag 1141 ctgttcgcct gtcgccgcgg caacgcctgc ttcctcggcg cctccccgga acgcctggtc 1201 cgcattcgcg ccggcgaggc actcacccat gccctggccg ggaccatcgc ccgcggcggc 1261 gatgcccagg aagatgcgcg gctcggacag gccctgctgg acagcgccaa ggacaggcac 1321 gaacaccagt tggtggtgga ggcgatccgt acggccctgg aacccttcag cgaggtgctg 1381 gaaatccccg atgcgcccgg cctgaaacga ctggcgcgag tccagcacct gaacacgccg 1441 atccgcgccc gcctcgctga cgcaggcggc atcctgcggc tgctacaagc gctgcatccg 1501 acccccgcgg tgggcggcta cccacgcagc gcggcgctgg actacatccg ccagcacgaa 1561 gggatggacc gcggctggta cgccgcgccg ctgggctggc tcgacggcga aggcaacggc 1621 gatttcctgg tggcgctgcg ctcggccctg ctcacgccgg gccggggcta cctgttcgcc 1681 ggctgcggtc tggtaggcga ttcggaaccg gcccacgagt atcgcgaaac ctgccttaag 1741 ctcagtgcca tgcgggaagc tctatccgcc ataggcggcc tggacgaagt gcccttgcag 1801 cgcggcgtcg cctaataata ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa 1861 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac 1921 tggctcacct tcgggtgggc ctttctgcgt ttatatacta gaggcttatg ttttcgctga 1981 tatcccgagc ggtttcaaaa ttgtgatcta tatttaacaa tactagagaa agaggggaaa 2041 tactagatgt ccagattaga taaaagtaaa gtgattaaca gcgcattaga gctgcttaat 2101 gaggtcggaa tcgaaggttt aacaacccgt aaactcgccc agaagctagg tgtagagcag 2161 cctacattgt attggcatgt aaaaaataag cgggctttgc tcgacgcctt agccattgag 2221 atgttagata ggcaccatac tcacttttgc cctttagaag gggaaagctg gcaagatttt 2281 ttacgtaata acgctaaaag ttttagatgt gctttactaa gtcatcgcga tggagcaaaa 2341 gtacatttag gtacacggcc tacagaaaaa cagtatgaaa ctctcgaaaa tcaattagcc 2401 tttttatgcc aacaaggttt ttcactagag aatgcattat atgcactcag cgctgtgggg 2461 cattttactt taggttgcgt attggaagat caagagcatc aagtcgctaa agaagaaagg 2521 gaaacaccta ctactgatag tatgccgcca ttattacgac aagctatcga attatttgat 2581 caccaaggtg cagagccagc cttcttattc ggccttgaat tgatcatatg cggattagaa 2641 aaacaactta aatgtgaaag tgggtccgct gcaaacgacg aaaactacgc tttagtagct 2701 taataacact gatagtgcta gtgtagatca ctactagagc caggcatcaa ataaaacgaa 2761 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct 2821 actagagtca cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagtccc 2881 tatcagtgat agagattgac atccctatca gtgatagaga tactgagcac tactagagtc 2941 acacaggaaa gtactagatg gaagttgttg aagttcttca catgaatgga ggaaatggag 3001 acagtagcta tgcaaacaat tctttggttc agcaaaaggt gattctcatg acaaagccaa 3061 taactgagca agccatgatt gatctctaca gcagcctctt tccagaaacc ttatgcattg 3121 cagatttggg ttgttctttg ggagctaaca ctttcttggt ggtctcacag cttgttaaaa 3181 tagtagaaaa agaacgaaaa aagcatggtt ttaagtctcc agagttttat tttcacttca 3241 atgatcttcc tggcaatgat tttaatacac tttttcagtc actgggggca tttcaagaag 3301 atttgagaaa gcatataggg gaaagctttg gtccatgttt tttcagtgga gtgcctggtt 3361 cattttatac tagacttttc ccttccaaaa gtttacattt tgtttactcc tcctacagtc 3421 tcatgtggct atctcaggtg cctaatggga ttgaaaataa caagggaaac atttacatgg 3481 caagaacaag ccctctaagt gttattaaag catactacaa gcaatatgaa atagattttt 3541 caaattttct caagtaccgt tcagaggaat tgatgaaagg tggaaagatg gtgttaacac 3601 tcctaggtag agaaagtgag gatcctacta gcaaagaatg ctgttacatt tgggagcttc 3661 tagccatggc cctcaataag ttggttgaag agggattgat aaaagaagag aaagtagatg 3721 cattcaatat tcctcaatac acaccatcac cagcagaagt aaagtacata gttgagaagg 3781 aaggatcatt caccattaat cgcttggaaa catcaagagt tcattggaat gcttctaata 3841 atgagaagaa tggtggttac aatgtgtcaa ggtgcatgag agctgtggct gagcctttgc 3901 ttgtcagcca ctttgacaag gaattgatgg atttagtgtt ccacaagtac gaagagattg 3961 tttctgattg catgtccaaa gagaatactg agtttataaa tgtcatcatc tccttgacca 4021 aaataaatta atactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg 4081 gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca 4141 ccttcgggtg ggcctttctg cgtttata //