##gff-version 3
##sequence-region BBa_J45800 1 4168
BBa_J45800	.	engineered_region	1964	4168	.	+	0	ID=BBa_J45180;Name=BBa_J45180
BBa_J45800	.	ribosome_entry_site	2939	2951	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_J45180
BBa_J45800	.	engineered_region	2939	2951	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_J45800	.	ribosome_entry_site	2939	2951	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_J45800	.	ribosome_entry_site	2945	2948	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_J45800	.	CDS	2958	4031	.	+	0	ID=BBa_J45004;Name=BBa_J45004;Parent=BBa_J45180
BBa_J45800	.	start_codon	2958	2960	.	+	0	ID=annotation1880519;Name=start codon;Parent=BBa_J45004
BBa_J45800	.	stop_codon	4029	4031	.	+	0	ID=annotation1880520;Name=stop codon;Parent=BBa_J45004
BBa_J45800	.	CDS	2958	4031	.	+	0	ID=annotation1880517;Name=BMST1;Parent=BBa_J45004
BBa_J45800	.	terminator	4040	4119	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J45180
BBa_J45800	.	stem_loop	4051	4094	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J45800	.	engineered_region	4040	4119	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J45800	.	engineered_region	2029	2930	.	+	0	ID=BBa_Q04401;Name=BBa_Q04401;Parent=BBa_J45180
BBa_J45800	.	ribosome_entry_site	2029	2040	.	+	0	ID=BBa_B0064;Name=BBa_B0064;Parent=BBa_Q04401
BBa_J45800	.	CDS	2047	2731	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_Q04401
BBa_J45800	.	engineered_tag	2707	2731	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_J45800	.	CDS	2050	2666	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_J45800	.	tag	2667	2700	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_J45800	.	terminator	2740	2868	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_Q04401
BBa_J45800	.	terminator	2740	2819	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_J45800	.	stem_loop	2751	2794	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J45800	.	engineered_region	2740	2819	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J45800	.	terminator	2828	2868	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_J45800	.	stop_codon	2861	2861	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J45800	.	stem_loop	2835	2854	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J45800	.	engineered_region	2828	2868	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J45800	.	polyA_site	2855	2868	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J45800	.	promoter	2877	2930	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_Q04401
BBa_J45800	.	promoter	2919	2924	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_J45800	.	promoter	2896	2901	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_J45800	.	non_covalent_binding_site	2902	2920	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_J45800	.	non_covalent_binding_site	2877	2895	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_J45800	.	engineered_region	2877	2930	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_J45800	.	promoter	1964	2020	.	+	0	ID=BBa_J45993;Name=BBa_J45993;Parent=BBa_J45180
BBa_J45800	.	promoter	2009	2014	.	+	0	ID=annotation1883647;Name=-10;Parent=BBa_J45993
BBa_J45800	.	promoter	1985	1990	.	+	0	ID=annotation1883648;Name=-35;Parent=BBa_J45993
BBa_J45800	.	terminator	4128	4168	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J45180
BBa_J45800	.	stop_codon	4161	4161	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J45800	.	stem_loop	4135	4154	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J45800	.	engineered_region	4128	4168	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J45800	.	polyA_site	4155	4168	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J45800	.	engineered_region	1	1955	.	+	0	ID=BBa_J45320;Name=BBa_J45320
BBa_J45800	.	terminator	1827	1906	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J45320
BBa_J45800	.	stem_loop	1838	1881	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J45800	.	engineered_region	1827	1906	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J45800	.	terminator	1915	1955	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J45320
BBa_J45800	.	stop_codon	1948	1948	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J45800	.	stem_loop	1922	1941	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J45800	.	engineered_region	1915	1955	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J45800	.	polyA_site	1942	1955	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J45800	.	promoter	1	54	.	+	0	ID=BBa_R0011;Name=BBa_R0011;Parent=BBa_J45320
BBa_J45800	.	promoter	43	48	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_J45800	.	operator	26	42	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_J45800	.	promoter	20	25	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_J45800	.	operator	3	19	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_J45800	.	engineered_region	1	54	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_J45800	.	CDS	83	1818	.	+	0	ID=BBa_J45017;Name=BBa_J45017;Parent=BBa_J45320
BBa_J45800	.	CDS	83	388	.	+	0	ID=annotation1961296;Name=PchB;Parent=BBa_J45017
BBa_J45800	.	CDS	385	1818	.	+	0	ID=annotation1961297;Name=PchA;Parent=BBa_J45017
BBa_J45800	.	stop_codon	386	388	.	+	0	ID=annotation1897456;Name=pchB stop codon;Parent=BBa_J45017
BBa_J45800	.	point_mutation	522	522	.	+	0	ID=annotation1897453;Name=g -> a to eliminate PstI site;Parent=BBa_J45017
BBa_J45800	.	stop_codon	1813	1818	.	+	0	ID=annotation1897457;Name=pchA double TAA stop codon;Parent=BBa_J45017
BBa_J45800	.	start_codon	385	387	.	+	0	ID=annotation1897454;Name=pchA start codon;Parent=BBa_J45017
BBa_J45800	.	start_codon	83	85	.	+	0	ID=annotation1897455;Name=pchB start codon;Parent=BBa_J45017
BBa_J45800	.	ribosome_entry_site	64	76	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_J45320
BBa_J45800	.	engineered_region	64	76	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_J45800	.	ribosome_entry_site	64	76	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_J45800	.	ribosome_entry_site	70	73	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
>BBa_J45800
aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagtcacacaggaaagtac
tagatgaaaactcccgaagactgcaccggcctggcggacatccgcgaggccatcgaccggatcgacctggatatcgtcc
aggccctcggccgccgcatggactacgtcaaggcggcgtcgcgcttcaaggccagcgaggcggcgattccggcgcccga
gcgggtcgccgcgatgctccccgagcgcgcccgctgggccgaggaaaacggactcgacgcgcccttcgtcgagggactg
ttcgcgcagatcatccactggtacatcgccgagcagatcaagtactggcgccagacacggggtgccgcatgagccggct
ggcgcccctgagccagtgcctgcacgccttgcgcggcaccttcgagcgcgccatcggccaggcgcaggcgctcgatcgt
ccggtgctggtggcggcatcgttcgagatcgacccattggacccgctacaggtattcggtgcctgggacgaccggcaaa
cgccctgcctgtactgggaacagcccgagctggcgttcttcgcctggggctgcgccctggagctgcaaggccacggcga
acagcgcttcgcccggatcgaggaaaactggcaattgctctgcgccgacgccgtggtcgagggcccgctggcgccgcgc
ctgtgcggcggattccgcttcgatccgcgcggcccgcgcgaggaacactggcaagccttcgccgatgccagcctgatgc
tcgccggcatcaccgtgctgcgcgagggcgaacgctaccgggtactctgccaacacctggccaagcccggcgaagatgc
cctggccctggccgcctaccactgctcggcgctactgcgcctgaggcagccggccagacgccggccctcggggccgacc
gctggcgcgcagggcgacgcttcggcgcaggagcgcaggcaatgggaagccaaggtgagcgacgcggtaagcagtgtcc
gccagggacgcttcggcaaggtcgtgctggcccgcacccaggcccggcctctcggcgacatcgagccgtggcaggtcat
cgaacacctgcgtctgcaacatgccgacgcccagctgttcgcctgtcgccgcggcaacgcctgcttcctcggcgcctcc
ccggaacgcctggtccgcattcgcgccggcgaggcactcacccatgccctggccgggaccatcgcccgcggcggcgatg
cccaggaagatgcgcggctcggacaggccctgctggacagcgccaaggacaggcacgaacaccagttggtggtggaggc
gatccgtacggccctggaacccttcagcgaggtgctggaaatccccgatgcgcccggcctgaaacgactggcgcgagtc
cagcacctgaacacgccgatccgcgcccgcctcgctgacgcaggcggcatcctgcggctgctacaagcgctgcatccga
cccccgcggtgggcggctacccacgcagcgcggcgctggactacatccgccagcacgaagggatggaccgcggctggta
cgccgcgccgctgggctggctcgacggcgaaggcaacggcgatttcctggtggcgctgcgctcggccctgctcacgccg
ggccggggctacctgttcgccggctgcggtctggtaggcgattcggaaccggcccacgagtatcgcgaaacctgcctta
agctcagtgccatgcgggaagctctatccgccataggcggcctggacgaagtgcccttgcagcgcggcgtcgcctaata
atactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggt
gaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagaggcttatgttttc
gctgatatcccgagcggtttcaaaattgtgatctatatttaacaatactagagaaagaggggaaatactagatgtccag
attagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaa
ctcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttag
ccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataa
cgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaa
cagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactca
gcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacc
tactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttctta
ttcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaact
acgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggct
cagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcacctt
cgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatac
tgagcactactagagtcacacaggaaagtactagatggaagttgttgaagttcttcacatgaatggaggaaatggagac
agtagctatgcaaacaattctttggttcagcaaaaggtgattctcatgacaaagccaataactgagcaagccatgattg
atctctacagcagcctctttccagaaaccttatgcattgcagatttgggttgttctttgggagctaacactttcttggt
ggtctcacagcttgttaaaatagtagaaaaagaacgaaaaaagcatggttttaagtctccagagttttattttcacttc
aatgatcttcctggcaatgattttaatacactttttcagtcactgggggcatttcaagaagatttgagaaagcatatag
gggaaagctttggtccatgttttttcagtggagtgcctggttcattttatactagacttttcccttccaaaagtttaca
ttttgtttactcctcctacagtctcatgtggctatctcaggtgcctaatgggattgaaaataacaagggaaacatttac
atggcaagaacaagccctctaagtgttattaaagcatactacaagcaatatgaaatagatttttcaaattttctcaagt
accgttcagaggaattgatgaaaggtggaaagatggtgttaacactcctaggtagagaaagtgaggatcctactagcaa
agaatgctgttacatttgggagcttctagccatggccctcaataagttggttgaagagggattgataaaagaagagaaa
gtagatgcattcaatattcctcaatacacaccatcaccagcagaagtaaagtacatagttgagaaggaaggatcattca
ccattaatcgcttggaaacatcaagagttcattggaatgcttctaataatgagaagaatggtggttacaatgtgtcaag
gtgcatgagagctgtggctgagcctttgcttgtcagccactttgacaaggaattgatggatttagtgttccacaagtac
gaagagattgtttctgattgcatgtccaaagagaatactgagtttataaatgtcatcatctccttgaccaaaataaatt
aatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcgg
tgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata