EWOBS
type: ComponentDefinition
persistent identity: BBa J45800
display id: BBa_J45800
version: 1
was generated by: iGEM to SBOL conversion
description: Exponential phase wintergreen odor biosynthetic system
created: 2006-10-31T12:00:00Z
modified: 2015-08-31T04:08:50Z
mutable provenance: This part is composed of <bbpart>BBa_J45320</bbpart> and <bbpart>BBa_J45180</bbpart>
owned by: james
owned by: myers
mutable description: This system designed by the MIT 2006 iGEM team generates mint smell by biosynthesizing salicylic acid from chassis-produced metabolites (chorismate) and then converts salicylic acid into methyl salicylate (wintergreen scent). It uses a stationary phase promoter followed by an inverter (see <bbpart>BBa_J45996<bbpart> for details) to only express the necessary enzymes during exponential phase.
discontinued: false
dominant: false
experience: experience/None
group u list: _84_
m user id: 0
owner id: 2
owning group id: 84
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable notes: We recommend use of strain <bbpart>BBa_J45999</bbpart> as it does not produce indole. Indole is responsible for the foul smell of wt e.coli, and masks the wintergreen scent produced by J45800.
star: false
creator: MIT iGEM 2006
type: DnaRegion
role: partType/Project
role: SO:0000804
component: BBa_J45180
component: BBa_J45320
sequence annotation: BBa_J45320
sequence annotation: BBa_J45180
sequence: https://synbiohub.org/public/igem/BBa_J45800_sequence/1

BBa_J45180
type: Component
persistent identity: component2291446
display id: component2291446
version: 1
top level: EWOBS
definition: BBa_J45180
access: public

BBa_J45320
type: Component
persistent identity: component2291428
display id: component2291428
version: 1
top level: EWOBS
definition: SG
access: public

BBa_J45320
type: SequenceAnnotation
persistent identity: annotation2291428
display id: annotation2291428
version: 1
top level: EWOBS
location: https://synbiohub.org/public/igem/BBa_J45800/annotation2291428/range2291428/1
component: BBa_J45320

https://synbiohub.org/public/igem/BBa_J45800/annotation2291428/range2291428/1
type: Range
persistent identity: range2291428
display id: range2291428
version: 1
top level: EWOBS
direction: direction/reverse
start: 1
end: 1955
orientation: inline

BBa_J45180
type: SequenceAnnotation
persistent identity: annotation2291446
display id: annotation2291446
version: 1
top level: EWOBS
location: https://synbiohub.org/public/igem/BBa_J45800/annotation2291446/range2291446/1
component: BBa_J45180

https://synbiohub.org/public/igem/BBa_J45800/annotation2291446/range2291446/1
type: Range
persistent identity: range2291446
display id: range2291446
version: 1
top level: EWOBS
direction: direction/reverse
start: 1964
end: 4168
orientation: inline

BBa_J45180
type: ComponentDefinition
persistent identity: BBa J45180
display id: BBa_J45180
version: 1
was generated by: iGEM to SBOL conversion
description: Exponential phase dependent wintergreen odor generator
created: 2006-10-30T12:00:00Z
modified: 2015-08-31T04:08:49Z
mutable provenance: -
owned by: james
owned by: myers
mutable description: This is a protein generator that outputs BSMT (benzoic acid/salicylic acid methyl transferase) under control of the osmY stationary phase promoter and Q04400 inverter. Ideally, when in stationary phase, BSMT output is "low" and vice versa.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _84_
m user id: 0
owner id: 977
owning group id: 84
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: -
star: false
creator: Andr?? Green II
type: DnaRegion
role: partType/Generator
role: SO:0000804
component: BBa_J45993
component: BBa_B0032
component: BBa_Q04401
component: BBa_B0010
component: BBa_J45004
component: BBa_B0012
sequence annotation: BBa_J45993
sequence annotation: BBa_Q04401
sequence annotation: BBa_B0032
sequence annotation: BBa_J45004
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_J45180_sequence/1

BBa_J45993
type: Component
persistent identity: component1909152
display id: component1909152
version: 1
top level: BBa_J45180
definition: p(osmY)
access: public

BBa_B0032
type: Component
persistent identity: component1909155
display id: component1909155
version: 1
top level: BBa_J45180
definition: BBa_B0032
access: public

BBa_Q04401
type: Component
persistent identity: component1909153
display id: component1909153
version: 1
top level: BBa_J45180
definition: tetR inver
access: public

BBa_B0010
type: Component
persistent identity: component1909161
display id: component1909161
version: 1
top level: BBa_J45180
definition: BBa_B0010
access: public

BBa_J45004
type: Component
persistent identity: component1909160
display id: component1909160
version: 1
top level: BBa_J45180
definition: BSMT1
access: public

BBa_B0012
type: Component
persistent identity: component1909163
display id: component1909163
version: 1
top level: BBa_J45180
definition: BBa_B0012
access: public

BBa_J45993
type: SequenceAnnotation
persistent identity: annotation1909152
display id: annotation1909152
version: 1
top level: BBa_J45180
location: https://synbiohub.org/public/igem/BBa_J45180/annotation1909152/range1909152/1
component: BBa_J45993

https://synbiohub.org/public/igem/BBa_J45180/annotation1909152/range1909152/1
type: Range
persistent identity: range1909152
display id: range1909152
version: 1
top level: BBa_J45180
direction: direction/reverse
start: 1
end: 57
orientation: inline

BBa_Q04401
type: SequenceAnnotation
persistent identity: annotation1909153
display id: annotation1909153
version: 1
top level: BBa_J45180
location: https://synbiohub.org/public/igem/BBa_J45180/annotation1909153/range1909153/1
component: BBa_Q04401

https://synbiohub.org/public/igem/BBa_J45180/annotation1909153/range1909153/1
type: Range
persistent identity: range1909153
display id: range1909153
version: 1
top level: BBa_J45180
direction: direction/reverse
start: 66
end: 967
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation1909155
display id: annotation1909155
version: 1
top level: BBa_J45180
location: https://synbiohub.org/public/igem/BBa_J45180/annotation1909155/range1909155/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_J45180/annotation1909155/range1909155/1
type: Range
persistent identity: range1909155
display id: range1909155
version: 1
top level: BBa_J45180
direction: direction/forward
start: 976
end: 988
orientation: inline

BBa_J45004
type: SequenceAnnotation
persistent identity: annotation1909160
display id: annotation1909160
version: 1
top level: BBa_J45180
location: https://synbiohub.org/public/igem/BBa_J45180/annotation1909160/range1909160/1
component: BBa_J45004

https://synbiohub.org/public/igem/BBa_J45180/annotation1909160/range1909160/1
type: Range
persistent identity: range1909160
display id: range1909160
version: 1
top level: BBa_J45180
direction: direction/reverse
start: 995
end: 2068
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1909161
display id: annotation1909161
version: 1
top level: BBa_J45180
location: https://synbiohub.org/public/igem/BBa_J45180/annotation1909161/range1909161/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J45180/annotation1909161/range1909161/1
type: Range
persistent identity: range1909161
display id: range1909161
version: 1
top level: BBa_J45180
direction: direction/forward
start: 2077
end: 2156
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1909163
display id: annotation1909163
version: 1
top level: BBa_J45180
location: https://synbiohub.org/public/igem/BBa_J45180/annotation1909163/range1909163/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J45180/annotation1909163/range1909163/1
type: Range
persistent identity: range1909163
display id: range1909163
version: 1
top level: BBa_J45180
direction: direction/forward
start: 2165
end: 2205
orientation: inline

SG
type: ComponentDefinition
persistent identity: BBa J45320
display id: BBa_J45320
version: 1
was generated by: iGEM to SBOL conversion
description: Salicylate generator
created: 2006-10-19T11:00:00Z
modified: 2015-08-31T04:08:49Z
mutable provenance: This part is phBSMT1 from Petunia x hybrida (GenBank). See part design and discussion for more information.
owned by: james
owned by: myers
mutable description: Protein generator converting PoPS to the prot BSMT. The BSMT enzyme converts benzoic acid/salicylic acid to methyl benzoate and methyl salicylate.
discontinued: false
dominant: false
experience: experience/Works
group u list: _84_
m user id: 0
owner id: 977
owning group id: 84
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: None
star: true
creator: Andr?? Green II
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0032
component: BBa_R0011
component: BBa_B0010
component: BBa_J45017
component: BBa_B0012
sequence annotation: BBa_R0011
sequence annotation: BBa_B0032
sequence annotation: BBa_J45017
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_J45320_sequence/1

BBa_B0032
type: Component
persistent identity: component1960951
display id: component1960951
version: 1
top level: SG
definition: BBa_B0032
access: public

BBa_R0011
type: Component
persistent identity: component1960945
display id: component1960945
version: 1
top level: SG
definition: lacI+pL
access: public

BBa_B0010
type: Component
persistent identity: component1960959
display id: component1960959
version: 1
top level: SG
definition: BBa_B0010
access: public

BBa_J45017
type: Component
persistent identity: component1960958
display id: component1960958
version: 1
top level: SG
definition: pchBA
access: public

BBa_B0012
type: Component
persistent identity: component1960961
display id: component1960961
version: 1
top level: SG
definition: BBa_B0012
access: public

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation1960945
display id: annotation1960945
version: 1
top level: SG
location: https://synbiohub.org/public/igem/BBa_J45320/annotation1960945/range1960945/1
component: BBa_R0011

https://synbiohub.org/public/igem/BBa_J45320/annotation1960945/range1960945/1
type: Range
persistent identity: range1960945
display id: range1960945
version: 1
top level: SG
direction: direction/forward
start: 1
end: 54
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation1960951
display id: annotation1960951
version: 1
top level: SG
location: https://synbiohub.org/public/igem/BBa_J45320/annotation1960951/range1960951/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_J45320/annotation1960951/range1960951/1
type: Range
persistent identity: range1960951
display id: range1960951
version: 1
top level: SG
direction: direction/forward
start: 64
end: 76
orientation: inline

BBa_J45017
type: SequenceAnnotation
persistent identity: annotation1960958
display id: annotation1960958
version: 1
top level: SG
location: https://synbiohub.org/public/igem/BBa_J45320/annotation1960958/range1960958/1
component: BBa_J45017

https://synbiohub.org/public/igem/BBa_J45320/annotation1960958/range1960958/1
type: Range
persistent identity: range1960958
display id: range1960958
version: 1
top level: SG
direction: direction/reverse
start: 83
end: 1818
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1960959
display id: annotation1960959
version: 1
top level: SG
location: https://synbiohub.org/public/igem/BBa_J45320/annotation1960959/range1960959/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J45320/annotation1960959/range1960959/1
type: Range
persistent identity: range1960959
display id: range1960959
version: 1
top level: SG
direction: direction/forward
start: 1827
end: 1906
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1960961
display id: annotation1960961
version: 1
top level: SG
location: https://synbiohub.org/public/igem/BBa_J45320/annotation1960961/range1960961/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J45320/annotation1960961/range1960961/1
type: Range
persistent identity: range1960961
display id: range1960961
version: 1
top level: SG
direction: direction/forward
start: 1915
end: 1955
orientation: inline

p(osmY)
type: ComponentDefinition
persistent identity: BBa J45993
display id: BBa_J45993
version: 1
was generated by: iGEM to SBOL conversion
description: Minimal stationary phase osmY promoter
created: 2006-07-10T11:00:00Z
modified: 2015-08-31T04:08:50Z
mutable provenance: This part was PCRed out of E. coli's genome.
owned by: james
owned by: myers
mutable description: osmY's promoter is active in stationary phase and under high osmotic pressure conditions.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _84_
m user id: 0
owner id: 642
owning group id: 84
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable notes: Truncated Forward Primer: 5'- GTT TCT TCG AAT TCG CGG CCG CTT CTA GGCT TAT GTT TTC GCT GAT ATC - 3' Total Length: 49 bp Annealing Length: 21 bp GC Content: 38.1% Melting Temperature: 49.5 degrees C hairpin deltaG: -2.66 kcal/mol self dimer deltaG: -99.97 kcal/mol Reverse Primer: 5'-GTT TCT TCC TGC AGC GGC CGC TAC TAG TAT TGT TAA ATA TAG ATC ACA ATT TTG- 3' Total Length: 54 bp Annealing Length: 25 bp GC Content: 20.0% Melting Temperature: 46.4 degrees C hairpin deltaG: -2.86 kcal/mol self dimer deltaG: -99.44 kcal/mol heterodimer deltaG with Conserved Forward Primer: -99.97 kcal/mol
star: false
creator: Stephen Payne
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_J45993_sequence/1

-35
type: SequenceAnnotation
persistent identity: annotation1883648
display id: annotation1883648
version: 1
top level: p(osmY)
location: https://synbiohub.org/public/igem/BBa_J45993/annotation1883648/range1883648/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_J45993/annotation1883648/range1883648/1
type: Range
persistent identity: range1883648
display id: range1883648
version: 1
top level: p(osmY)
direction: direction/forward
start: 22
end: 27
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1883647
display id: annotation1883647
version: 1
top level: p(osmY)
location: https://synbiohub.org/public/igem/BBa_J45993/annotation1883647/range1883647/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_J45993/annotation1883647/range1883647/1
type: Range
persistent identity: range1883647
display id: range1883647
version: 1
top level: p(osmY)
direction: direction/forward
start: 46
end: 51
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0032
type: ComponentDefinition
persistent identity: BBa B0032
display id: BBa_B0032
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.3 (medium) -- derivative of BBa_0030
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_44_48_46_1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: RBS-3\Weak
sequence annotation: BBa_B0032
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0032_sequence/1

RBS-3\Weak
type: SequenceAnnotation
persistent identity: annotation1709
display id: annotation1709
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
type: Range
persistent identity: range1709
display id: range1709
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation7027
display id: annotation7027
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
type: Range
persistent identity: range7027
display id: range7027
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1710
display id: annotation1710
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
type: Range
persistent identity: range1710
display id: range1710
version: 1
top level: BBa_B0032
direction: direction/forward
start: 7
end: 10
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

lacI+pL
type: ComponentDefinition
persistent identity: BBa R0011
display id: BBa_R0011
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (lacI regulated, lambda pL hybrid)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: represillator of Elowitz and Leibler (2000)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The PLlac 0-1 promoter is a hybrid regulatory region consisting of the promoter P(L) of phage lambda with the cI binding sites replaced with lacO1. The hybrid design allows for strong promotion that can nevertheless be tightly repressed by LacI, the Lac inhibitor (i.e. repressor) (<bb_part>BBa_C0010</bb_part>) ([LUTZ97]). The activity of the promoter can be regulated over a >600-fold range by IPTG in E.Coli DH5-alpha-Z1 (same paper reference).
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: hybrid promoter design to create strong promoter that is, at the same time, highly repressible. note that the upstream operator installed in this hybrid is slightly different than the one in the original source (Lutz and Bujard, 1997). the most upstream operator region is slightly truncated in the represillator version, so that both operators in the hybrid are the same sequence. see references for details. also, the sequence has been truncated after the transcriptional start site. LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and increase transcription. This part is incompatible with environments containing lactose or lactose analogs.
star: true
creator: Neelaksh Varshney, Grace Kenney, Daniel Shen, Samantha Sutton
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0011
sequence annotation: lac O1
sequence annotation: -35
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0011_sequence/1

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation7064
display id: annotation7064
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation7064/range7064/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0011/annotation7064/range7064/1
type: Range
persistent identity: range7064
display id: range7064
version: 1
top level: lacI+pL
direction: direction/forward
start: 1
end: 54
orientation: inline

lac O1
type: SequenceAnnotation
persistent identity: annotation1999
display id: annotation1999
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation1999/range1999/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_R0011/annotation1999/range1999/1
type: Range
persistent identity: range1999
display id: range1999
version: 1
top level: lacI+pL
direction: direction/forward
start: 3
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2000
display id: annotation2000
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2000/range2000/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0011/annotation2000/range2000/1
type: Range
persistent identity: range2000
display id: range2000
version: 1
top level: lacI+pL
direction: direction/forward
start: 20
end: 25
orientation: inline

lac O1
type: SequenceAnnotation
persistent identity: annotation2001
display id: annotation2001
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2001/range2001/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_R0011/annotation2001/range2001/1
type: Range
persistent identity: range2001
display id: range2001
version: 1
top level: lacI+pL
direction: direction/forward
start: 26
end: 42
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2002
display id: annotation2002
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2002/range2002/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0011/annotation2002/range2002/1
type: Range
persistent identity: range2002
display id: range2002
version: 1
top level: lacI+pL
direction: direction/forward
start: 43
end: 48
orientation: inline

tetR inver
type: ComponentDefinition
persistent identity: BBa Q04401
display id: BBa_Q04401
version: 1
was generated by: iGEM to SBOL conversion
description: TetR-derived transcriptional inverter
created: 2006-05-10T11:00:00Z
modified: 2015-05-08T01:14:13Z
owned by: james
owned by: myers
mutable description: Currently entered as a placeholder. This is a modified version of the tetR inverter Q04400 with a single base switch in the RBS: AAAGAGGAGAAA to AAAGAGGGGAAA
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Josh Michener
type: DnaRegion
role: SO:0000804
role: partType/Inverter
component: BBa_B0064
component: BBa_B0015
component: BBa_C0040
component: BBa_R0040
sequence annotation: BBa_B0064
sequence annotation: BBa_C0040
sequence annotation: BBa_B0015
sequence annotation: BBa_R0040
sequence: https://synbiohub.org/public/igem/BBa_Q04401_sequence/1

BBa_B0064
type: Component
persistent identity: component2251459
display id: component2251459
version: 1
top level: tetR inver
definition: BBa_B0064
access: public

BBa_B0015
type: Component
persistent identity: component2251470
display id: component2251470
version: 1
top level: tetR inver
definition: BBa_B0015
access: public

BBa_C0040
type: Component
persistent identity: component2251463
display id: component2251463
version: 1
top level: tetR inver
definition: tetR
access: public

BBa_R0040
type: Component
persistent identity: component2251471
display id: component2251471
version: 1
top level: tetR inver
definition: p(tetR)
access: public

BBa_B0064
type: SequenceAnnotation
persistent identity: annotation2251459
display id: annotation2251459
version: 1
top level: tetR inver
location: https://synbiohub.org/public/igem/BBa_Q04401/annotation2251459/range2251459/1
component: BBa_B0064

https://synbiohub.org/public/igem/BBa_Q04401/annotation2251459/range2251459/1
type: Range
persistent identity: range2251459
display id: range2251459
version: 1
top level: tetR inver
direction: direction/reverse
start: 1
end: 12
orientation: inline

BBa_C0040
type: SequenceAnnotation
persistent identity: annotation2251463
display id: annotation2251463
version: 1
top level: tetR inver
location: https://synbiohub.org/public/igem/BBa_Q04401/annotation2251463/range2251463/1
component: BBa_C0040

https://synbiohub.org/public/igem/BBa_Q04401/annotation2251463/range2251463/1
type: Range
persistent identity: range2251463
display id: range2251463
version: 1
top level: tetR inver
direction: direction/reverse
start: 19
end: 703
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2251470
display id: annotation2251470
version: 1
top level: tetR inver
location: https://synbiohub.org/public/igem/BBa_Q04401/annotation2251470/range2251470/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_Q04401/annotation2251470/range2251470/1
type: Range
persistent identity: range2251470
display id: range2251470
version: 1
top level: tetR inver
direction: direction/reverse
start: 712
end: 840
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation2251471
display id: annotation2251471
version: 1
top level: tetR inver
location: https://synbiohub.org/public/igem/BBa_Q04401/annotation2251471/range2251471/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_Q04401/annotation2251471/range2251471/1
type: Range
persistent identity: range2251471
display id: range2251471
version: 1
top level: tetR inver
direction: direction/forward
start: 849
end: 902
orientation: inline

pchBA
type: ComponentDefinition
persistent identity: BBa J45017
display id: BBa_J45017
version: 1
was generated by: iGEM to SBOL conversion
description: isochorismate pyruvate-lyase and isochorismate synthase (pchBA); converts chorismate to salicylate
created: 2006-08-20T11:00:00Z
modified: 2015-08-31T04:08:49Z
mutable provenance: pchBA is found in pseudomonas. The plasmid was kindly sent to the MIT iGEM team by Dr. Cornelia Riemmann from the Universite de Lausanne.
owned by: james
owned by: myers
mutable description: pchBA codes for proteins used to generate salicylic acid. It doesn't make a fusion protein, even though the coding regions overlap; the RBS for pchA is located at the end of the coding region of pchB.
discontinued: false
dominant: false
experience: experience/Works
group u list: _84_
m user id: 0
owner id: 1147
owning group id: 84
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: n/a
star: false
creator: MIT IGEM 2006
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: pchB start codon
sequence annotation: PchB
sequence annotation: pchA start codon
sequence annotation: PchA
sequence annotation: pchB stop codon
sequence annotation: g -> a to eliminate PstI site
sequence annotation: pchA double TAA stop codon
sequence: https://synbiohub.org/public/igem/BBa_J45017_sequence/1

pchB start codon
type: SequenceAnnotation
persistent identity: annotation1897455
display id: annotation1897455
version: 1
top level: pchBA
location: https://synbiohub.org/public/igem/BBa_J45017/annotation1897455/range1897455/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_J45017/annotation1897455/range1897455/1
type: Range
persistent identity: range1897455
display id: range1897455
version: 1
top level: pchBA
direction: direction/forward
start: 1
end: 3
orientation: inline

PchB
type: SequenceAnnotation
persistent identity: annotation1961296
display id: annotation1961296
version: 1
top level: pchBA
location: https://synbiohub.org/public/igem/BBa_J45017/annotation1961296/range1961296/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_J45017/annotation1961296/range1961296/1
type: Range
persistent identity: range1961296
display id: range1961296
version: 1
top level: pchBA
direction: direction/forward
start: 1
end: 306
orientation: inline

pchA start codon
type: SequenceAnnotation
persistent identity: annotation1897454
display id: annotation1897454
version: 1
top level: pchBA
location: https://synbiohub.org/public/igem/BBa_J45017/annotation1897454/range1897454/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_J45017/annotation1897454/range1897454/1
type: Range
persistent identity: range1897454
display id: range1897454
version: 1
top level: pchBA
direction: direction/forward
start: 303
end: 305
orientation: inline

PchA
type: SequenceAnnotation
persistent identity: annotation1961297
display id: annotation1961297
version: 1
top level: pchBA
location: https://synbiohub.org/public/igem/BBa_J45017/annotation1961297/range1961297/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_J45017/annotation1961297/range1961297/1
type: Range
persistent identity: range1961297
display id: range1961297
version: 1
top level: pchBA
direction: direction/forward
start: 303
end: 1736
orientation: inline

pchB stop codon
type: SequenceAnnotation
persistent identity: annotation1897456
display id: annotation1897456
version: 1
top level: pchBA
location: https://synbiohub.org/public/igem/BBa_J45017/annotation1897456/range1897456/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_J45017/annotation1897456/range1897456/1
type: Range
persistent identity: range1897456
display id: range1897456
version: 1
top level: pchBA
direction: direction/forward
start: 304
end: 306
orientation: inline

g -> a to eliminate PstI site
type: SequenceAnnotation
persistent identity: annotation1897453
display id: annotation1897453
version: 1
top level: pchBA
location: https://synbiohub.org/public/igem/BBa_J45017/annotation1897453/range1897453/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_J45017/annotation1897453/range1897453/1
type: Range
persistent identity: range1897453
display id: range1897453
version: 1
top level: pchBA
direction: direction/forward
start: 440
end: 440
orientation: inline

pchA double TAA stop codon
type: SequenceAnnotation
persistent identity: annotation1897457
display id: annotation1897457
version: 1
top level: pchBA
location: https://synbiohub.org/public/igem/BBa_J45017/annotation1897457/range1897457/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_J45017/annotation1897457/range1897457/1
type: Range
persistent identity: range1897457
display id: range1897457
version: 1
top level: pchBA
direction: direction/forward
start: 1731
end: 1736
orientation: inline

BSMT1
type: ComponentDefinition
persistent identity: BBa J45004
display id: BBa_J45004
version: 1
was generated by: iGEM to SBOL conversion
description: SAM:benzoic acid/salicylic acid carboxyl methyltransferase I; converts salicylic acid to methyl sali
created: 2006-06-06T11:00:00Z
modified: 2015-08-31T04:08:49Z
mutable provenance: Species: Petunia x hybrida from Genbank; (also available: A. thaliana and N. suaveolens)
owned by: james
owned by: myers
mutable description: Codes for BSMT enzyme to convert Benzoic acid/salicylic acid to methyl benzoate and methyl salicylate. This part is phBSMT1 from Petunia x hybrida.
discontinued: false
dominant: false
experience: experience/Works
group u list: _84_
m user id: 0
owner id: 974
owning group id: 84
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: Transport issues are being considered.
star: true
creator: Boyuan Zhu
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: BMST1
sequence annotation: start codon
sequence annotation: stop codon
sequence: https://synbiohub.org/public/igem/BBa_J45004_sequence/1

BMST1
type: SequenceAnnotation
persistent identity: annotation1880517
display id: annotation1880517
version: 1
top level: BSMT1
location: https://synbiohub.org/public/igem/BBa_J45004/annotation1880517/range1880517/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_J45004/annotation1880517/range1880517/1
type: Range
persistent identity: range1880517
display id: range1880517
version: 1
top level: BSMT1
direction: direction/forward
start: 1
end: 1074
orientation: inline

start codon
type: SequenceAnnotation
persistent identity: annotation1880519
display id: annotation1880519
version: 1
top level: BSMT1
location: https://synbiohub.org/public/igem/BBa_J45004/annotation1880519/range1880519/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_J45004/annotation1880519/range1880519/1
type: Range
persistent identity: range1880519
display id: range1880519
version: 1
top level: BSMT1
direction: direction/forward
start: 1
end: 3
orientation: inline

stop codon
type: SequenceAnnotation
persistent identity: annotation1880520
display id: annotation1880520
version: 1
top level: BSMT1
location: https://synbiohub.org/public/igem/BBa_J45004/annotation1880520/range1880520/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_J45004/annotation1880520/range1880520/1
type: Range
persistent identity: range1880520
display id: range1880520
version: 1
top level: BSMT1
direction: direction/forward
start: 1072
end: 1074
orientation: inline

p(tetR)
type: ComponentDefinition
persistent identity: BBa R0040
display id: BBa_R0040
version: 1
was generated by: iGEM to SBOL conversion
description: TetR repressible promoter
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: Lutz, R., Bujard, H., Nucleic Acids Research (1997) 25, 1203-1210.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Sequence for pTet inverting regulator driven by the TetR protein.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0040
sequence annotation: TetR 1
sequence annotation: -35
sequence annotation: TetR 2
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0040_sequence/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1986784
display id: annotation1986784
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
type: Range
persistent identity: range1986784
display id: range1986784
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 54
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation1986783
display id: annotation1986783
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
type: Range
persistent identity: range1986783
display id: range1986783
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1986785
display id: annotation1986785
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
type: Range
persistent identity: range1986785
display id: range1986785
version: 1
top level: p(tetR)
direction: direction/forward
start: 20
end: 25
orientation: inline

TetR 2
type: SequenceAnnotation
persistent identity: annotation1986786
display id: annotation1986786
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
type: Range
persistent identity: range1986786
display id: range1986786
version: 1
top level: p(tetR)
direction: direction/forward
start: 26
end: 44
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1986787
display id: annotation1986787
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
type: Range
persistent identity: range1986787
display id: range1986787
version: 1
top level: p(tetR)
direction: direction/forward
start: 43
end: 48
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

tetR
type: ComponentDefinition
persistent identity: BBa C0040
display id: BBa_C0040
version: 1
was generated by: iGEM to SBOL conversion
description: tetracycline repressor from transposon Tn10 (+LVA)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Coding region for the TetR protein without the Ribosome Binding Site. Modified with an LVA tail for rapid degradation of the protein and faster fall time for the emission. TetR binds to the pTet regulator (BBa_R0040). aTc (anhydrotetracycline) binds to TetR and inhibits its operation.
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: References (unparsed) here: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999). Lutz R, Bujard H., Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 1997 Mar 15;25(6):1203-10. PMID: 9092630 References (unparsed) here: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999). Lutz R, Bujard H., Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 1997 Mar 15;25(6):1203-10. PMID: 9092630 BBa_C0040 TetR Protein is based on the TetR sequence from Elowitz's repressilator. It has been modified to include a rapid degradation LVA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman.
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: tetR
sequence annotation: SsrA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0040_sequence/1

tetR
type: SequenceAnnotation
persistent identity: annotation23329
display id: annotation23329
version: 1
top level: tetR
location: https://synbiohub.org/public/igem/BBa_C0040/annotation23329/range23329/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_C0040/annotation23329/range23329/1
type: Range
persistent identity: range23329
display id: range23329
version: 1
top level: tetR
direction: direction/forward
start: 4
end: 620
orientation: inline

SsrA
type: SequenceAnnotation
persistent identity: annotation23330
display id: annotation23330
version: 1
top level: tetR
location: https://synbiohub.org/public/igem/BBa_C0040/annotation23330/range23330/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_C0040/annotation23330/range23330/1
type: Range
persistent identity: range23330
display id: range23330
version: 1
top level: tetR
direction: direction/forward
start: 621
end: 654
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213989
display id: annotation2213989
version: 1
top level: tetR
location: https://synbiohub.org/public/igem/BBa_C0040/annotation2213989/range2213989/1
role: feature/barcode
role: SO:0000807

https://synbiohub.org/public/igem/BBa_C0040/annotation2213989/range2213989/1
type: Range
persistent identity: range2213989
display id: range2213989
version: 1
top level: tetR
direction: direction/forward
start: 661
end: 685
orientation: inline

BBa_B0064
type: ComponentDefinition
persistent identity: BBa B0064
display id: BBa_B0064
version: 1
was generated by: iGEM to SBOL conversion
description: Tuned RBS for Q04401
created: 2008-02-23T12:00:00Z
modified: 2015-08-31T04:07:21Z
mutable provenance: .
owned by: james
owned by: myers
mutable description: This is a single bp change from B0034. It's strength is approximately 35% that of B0034.
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 2
owning group id: 84
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: .
star: false
creator: Jason Kelly
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_B0064_sequence/1

https://synbiohub.org/public/igem/BBa_J45800_sequence/1
type: Sequence
persistent identity: BBa J45800 sequence
display id: BBa_J45800_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagtcacacaggaaagtactagatgaaaactcccgaagactgcaccggcctggcggacatccgcgaggccatcgaccggatcgacctggatatcgtccaggccctcggccgccgcatggactacgtcaaggcggcgtcgcgcttcaaggccagcgaggcggcgattccggcgcccgagcgggtcgccgcgatgctccccgagcgcgcccgctgggccgaggaaaacggactcgacgcgcccttcgtcgagggactgttcgcgcagatcatccactggtacatcgccgagcagatcaagtactggcgccagacacggggtgccgcatgagccggctggcgcccctgagccagtgcctgcacgccttgcgcggcaccttcgagcgcgccatcggccaggcgcaggcgctcgatcgtccggtgctggtggcggcatcgttcgagatcgacccattggacccgctacaggtattcggtgcctgggacgaccggcaaacgccctgcctgtactgggaacagcccgagctggcgttcttcgcctggggctgcgccctggagctgcaaggccacggcgaacagcgcttcgcccggatcgaggaaaactggcaattgctctgcgccgacgccgtggtcgagggcccgctggcgccgcgcctgtgcggcggattccgcttcgatccgcgcggcccgcgcgaggaacactggcaagccttcgccgatgccagcctgatgctcgccggcatcaccgtgctgcgcgagggcgaacgctaccgggtactctgccaacacctggccaagcccggcgaagatgccctggccctggccgcctaccactgctcggcgctactgcgcctgaggcagccggccagacgccggccctcggggccgaccgctggcgcgcagggcgacgcttcggcgcaggagcgcaggcaatgggaagccaaggtgagcgacgcggtaagcagtgtccgccagggacgcttcggcaaggtcgtgctggcccgcacccaggcccggcctctcggcgacatcgagccgtggcaggtcatcgaacacctgcgtctgcaacatgccgacgcccagctgttcgcctgtcgccgcggcaacgcctgcttcctcggcgcctccccggaacgcctggtccgcattcgcgccggcgaggcactcacccatgccctggccgggaccatcgcccgcggcggcgatgcccaggaagatgcgcggctcggacaggccctgctggacagcgccaaggacaggcacgaacaccagttggtggtggaggcgatccgtacggccctggaacccttcagcgaggtgctggaaatccccgatgcgcccggcctgaaacgactggcgcgagtccagcacctgaacacgccgatccgcgcccgcctcgctgacgcaggcggcatcctgcggctgctacaagcgctgcatccgacccccgcggtgggcggctacccacgcagcgcggcgctggactacatccgccagcacgaagggatggaccgcggctggtacgccgcgccgctgggctggctcgacggcgaaggcaacggcgatttcctggtggcgctgcgctcggccctgctcacgccgggccggggctacctgttcgccggctgcggtctggtaggcgattcggaaccggcccacgagtatcgcgaaacctgccttaagctcagtgccatgcgggaagctctatccgccataggcggcctggacgaagtgcccttgcagcgcggcgtcgcctaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagaggcttatgttttcgctgatatcccgagcggtttcaaaa…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J45180_sequence/1
type: Sequence
persistent identity: BBa J45180 sequence
display id: BBa_J45180_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcttatgttttcgctgatatcccgagcggtttcaaaattgtgatctatatttaacaatactagagaaagaggggaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagtcacacaggaaagtactagatggaagttgttgaagttcttcacatgaatggaggaaatggagacagtagctatgcaaacaattctttggttcagcaaaaggtgattctcatgacaaagccaataactgagcaagccatgattgatctctacagcagcctctttccagaaaccttatgcattgcagatttgggttgttctttgggagctaacactttcttggtggtctcacagcttgttaaaatagtagaaaaagaacgaaaaaagcatggttttaagtctccagagttttattttcacttcaatgatcttcctggcaatgattttaatacactttttcagtcactgggggcatttcaagaagatttgagaaagcatataggggaaagctttggtccatgttttttcagtggagtgcctggttcattttatactagacttttcccttccaaaagtttacattttgtttactcctcctacagtctcatgtggctatctcaggtgcctaatgggattgaaaataacaagggaaacatttacatggcaagaacaagccctctaagtgttattaaagcatactacaagcaatatgaaatagatttttcaaattttctcaagtaccgttcagaggaattgatgaaaggtggaaagatggtgttaacactcctaggtagagaaagtgaggatcctactagcaaagaatgctgttacatttgggagcttctagccatggccctcaataagttggttgaagagggattgataaaagaagagaaagtagatgcattcaatattcctcaatacacaccatcaccagcagaagtaaagtacatagttgagaaggaaggatcattcaccattaatcgcttggaaacatcaagagttcattggaatgcttctaataatgagaagaatggtggttacaatgtgtcaaggtgcatgagagctgtggctgagcctttgcttgtcagccactttgacaaggaattgatggatttagtgttccacaagtacgaagagattgttt…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J45320_sequence/1
type: Sequence
persistent identity: BBa J45320 sequence
display id: BBa_J45320_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagtcacacaggaaagtactagatgaaaactcccgaagactgcaccggcctggcggacatccgcgaggccatcgaccggatcgacctggatatcgtccaggccctcggccgccgcatggactacgtcaaggcggcgtcgcgcttcaaggccagcgaggcggcgattccggcgcccgagcgggtcgccgcgatgctccccgagcgcgcccgctgggccgaggaaaacggactcgacgcgcccttcgtcgagggactgttcgcgcagatcatccactggtacatcgccgagcagatcaagtactggcgccagacacggggtgccgcatgagccggctggcgcccctgagccagtgcctgcacgccttgcgcggcaccttcgagcgcgccatcggccaggcgcaggcgctcgatcgtccggtgctggtggcggcatcgttcgagatcgacccattggacccgctacaggtattcggtgcctgggacgaccggcaaacgccctgcctgtactgggaacagcccgagctggcgttcttcgcctggggctgcgccctggagctgcaaggccacggcgaacagcgcttcgcccggatcgaggaaaactggcaattgctctgcgccgacgccgtggtcgagggcccgctggcgccgcgcctgtgcggcggattccgcttcgatccgcgcggcccgcgcgaggaacactggcaagccttcgccgatgccagcctgatgctcgccggcatcaccgtgctgcgcgagggcgaacgctaccgggtactctgccaacacctggccaagcccggcgaagatgccctggccctggccgcctaccactgctcggcgctactgcgcctgaggcagccggccagacgccggccctcggggccgaccgctggcgcgcagggcgacgcttcggcgcaggagcgcaggcaatgggaagccaaggtgagcgacgcggtaagcagtgtccgccagggacgcttcggcaaggtcgtgctggcccgcacccaggcccggcctctcggcgacatcgagccgtggcaggtcatcgaacacctgcgtctgcaacatgccgacgcccagctgttcgcctgtcgccgcggcaacgcctgcttcctcggcgcctccccggaacgcctggtccgcattcgcgccggcgaggcactcacccatgccctggccgggaccatcgcccgcggcggcgatgcccaggaagatgcgcggctcggacaggccctgctggacagcgccaaggacaggcacgaacaccagttggtggtggaggcgatccgtacggccctggaacccttcagcgaggtgctggaaatccccgatgcgcccggcctgaaacgactggcgcgagtccagcacctgaacacgccgatccgcgcccgcctcgctgacgcaggcggcatcctgcggctgctacaagcgctgcatccgacccccgcggtgggcggctacccacgcagcgcggcgctggactacatccgccagcacgaagggatggaccgcggctggtacgccgcgccgctgggctggctcgacggcgaaggcaacggcgatttcctggtggcgctgcgctcggccctgctcacgccgggccggggctacctgttcgccggctgcggtctggtaggcgattcggaaccggcccacgagtatcgcgaaacctgccttaagctcagtgccatgcgggaagctctatccgccataggcggcctggacgaagtgcccttgcagcgcggcgtcgcctaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J45993_sequence/1
type: Sequence
persistent identity: BBa J45993 sequence
display id: BBa_J45993_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcttatgttttcgctgatatcccgagcggtttcaaaattgtgatctatatttaacaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0032_sequence/1
type: Sequence
persistent identity: BBa B0032 sequence
display id: BBa_B0032_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0011_sequence/1
type: Sequence
persistent identity: BBa R0011 sequence
display id: BBa_R0011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_Q04401_sequence/1
type: Sequence
persistent identity: BBa Q04401 sequence
display id: BBa_Q04401_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggggaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J45017_sequence/1
type: Sequence
persistent identity: BBa J45017 sequence
display id: BBa_J45017_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaaactcccgaagactgcaccggcctggcggacatccgcgaggccatcgaccggatcgacctggatatcgtccaggccctcggccgccgcatggactacgtcaaggcggcgtcgcgcttcaaggccagcgaggcggcgattccggcgcccgagcgggtcgccgcgatgctccccgagcgcgcccgctgggccgaggaaaacggactcgacgcgcccttcgtcgagggactgttcgcgcagatcatccactggtacatcgccgagcagatcaagtactggcgccagacacggggtgccgcatgagccggctggcgcccctgagccagtgcctgcacgccttgcgcggcaccttcgagcgcgccatcggccaggcgcaggcgctcgatcgtccggtgctggtggcggcatcgttcgagatcgacccattggacccgctacaggtattcggtgcctgggacgaccggcaaacgccctgcctgtactgggaacagcccgagctggcgttcttcgcctggggctgcgccctggagctgcaaggccacggcgaacagcgcttcgcccggatcgaggaaaactggcaattgctctgcgccgacgccgtggtcgagggcccgctggcgccgcgcctgtgcggcggattccgcttcgatccgcgcggcccgcgcgaggaacactggcaagccttcgccgatgccagcctgatgctcgccggcatcaccgtgctgcgcgagggcgaacgctaccgggtactctgccaacacctggccaagcccggcgaagatgccctggccctggccgcctaccactgctcggcgctactgcgcctgaggcagccggccagacgccggccctcggggccgaccgctggcgcgcagggcgacgcttcggcgcaggagcgcaggcaatgggaagccaaggtgagcgacgcggtaagcagtgtccgccagggacgcttcggcaaggtcgtgctggcccgcacccaggcccggcctctcggcgacatcgagccgtggcaggtcatcgaacacctgcgtctgcaacatgccgacgcccagctgttcgcctgtcgccgcggcaacgcctgcttcctcggcgcctccccggaacgcctggtccgcattcgcgccggcgaggcactcacccatgccctggccgggaccatcgcccgcggcggcgatgcccaggaagatgcgcggctcggacaggccctgctggacagcgccaaggacaggcacgaacaccagttggtggtggaggcgatccgtacggccctggaacccttcagcgaggtgctggaaatccccgatgcgcccggcctgaaacgactggcgcgagtccagcacctgaacacgccgatccgcgcccgcctcgctgacgcaggcggcatcctgcggctgctacaagcgctgcatccgacccccgcggtgggcggctacccacgcagcgcggcgctggactacatccgccagcacgaagggatggaccgcggctggtacgccgcgccgctgggctggctcgacggcgaaggcaacggcgatttcctggtggcgctgcgctcggccctgctcacgccgggccggggctacctgttcgccggctgcggtctggtaggcgattcggaaccggcccacgagtatcgcgaaacctgccttaagctcagtgccatgcgggaagctctatccgccataggcggcctggacgaagtgcccttgcagcgcggcgtcgcctaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J45004_sequence/1
type: Sequence
persistent identity: BBa J45004 sequence
display id: BBa_J45004_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggaagttgttgaagttcttcacatgaatggaggaaatggagacagtagctatgcaaacaattctttggttcagcaaaaggtgattctcatgacaaagccaataactgagcaagccatgattgatctctacagcagcctctttccagaaaccttatgcattgcagatttgggttgttctttgggagctaacactttcttggtggtctcacagcttgttaaaatagtagaaaaagaacgaaaaaagcatggttttaagtctccagagttttattttcacttcaatgatcttcctggcaatgattttaatacactttttcagtcactgggggcatttcaagaagatttgagaaagcatataggggaaagctttggtccatgttttttcagtggagtgcctggttcattttatactagacttttcccttccaaaagtttacattttgtttactcctcctacagtctcatgtggctatctcaggtgcctaatgggattgaaaataacaagggaaacatttacatggcaagaacaagccctctaagtgttattaaagcatactacaagcaatatgaaatagatttttcaaattttctcaagtaccgttcagaggaattgatgaaaggtggaaagatggtgttaacactcctaggtagagaaagtgaggatcctactagcaaagaatgctgttacatttgggagcttctagccatggccctcaataagttggttgaagagggattgataaaagaagagaaagtagatgcattcaatattcctcaatacacaccatcaccagcagaagtaaagtacatagttgagaaggaaggatcattcaccattaatcgcttggaaacatcaagagttcattggaatgcttctaataatgagaagaatggtggttacaatgtgtcaaggtgcatgagagctgtggctgagcctttgcttgtcagccactttgacaaggaattgatggatttagtgttccacaagtacgaagagattgtttctgattgcatgtccaaagagaatactgagtttataaatgtcatcatctccttgaccaaaataaattaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0040_sequence/1
type: Sequence
persistent identity: BBa R0040 sequence
display id: BBa_R0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0040_sequence/1
type: Sequence
persistent identity: BBa C0040 sequence
display id: BBa_C0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0064_sequence/1
type: Sequence
persistent identity: BBa B0064 sequence
display id: BBa_B0064_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggggaaa
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z