LOCUS BBa_J45900 4886 bp DNA linear 22-Dec-2024 DEFINITION Banana odor biosynthetic system ACCESSION BBa_J45900 VERSION BBa_J45900.1 FEATURES Location/Qualifiers misc_feature 3..19 /label=lac O1 promoter 20..25 /label=-35 misc_feature 26..42 /label=lac O1 promoter 43..48 /label=-10 misc_feature 1..54 /label=BBa_R0011 promoter 1..54 /label=BBa_R0011 RBS 64..78 /label=RBS-1\Strong RBS 71..75 /label=RBS misc_feature 64..78 /label=BBa_B0030 RBS 64..78 /label=BBa_B0030 misc_feature 85..87 /label=start codon misc_feature 1213..1218 /label=double TAA stop codon misc_feature 855..855 /label=a->g to eliminate SpeI CDS 85..1218 /label=BAT2 CDS 85..1218 /label=BBa_J45008 RBS 1227..1241 /label=RBS-1\Strong RBS 1234..1238 /label=RBS misc_feature 1227..1241 /label=BBa_B0030 RBS 1227..1241 /label=BBa_B0030 misc_feature 1253..1253 /label=t->c to eliminate EcoRI, to do misc_feature 2153..2153 /label=a->g to eliminate EcoRI, to do misc_feature 2852..2852 /label=a->g to eliminate EcoRI, to do misc_feature 3038..3038 /label=a->g to eliminate EcoRI, to do misc_feature 1946..1946 /label=t->c to eliminate SpeI, to do CDS 1248..3077 /label=THI3 misc_feature 1248..1250 /label=start codon misc_feature 3075..3077 /label=stop codon CDS 1248..3077 /label=BBa_J45009 misc_feature 3086..3104 /label=TetR 1 misc_feature 3086..3139 /label=BBa_R0040 promoter 3105..3110 /label=-35 misc_feature 3111..3129 /label=TetR 2 promoter 3128..3133 /label=-10 promoter 3086..3139 /label=BBa_R0040 RBS 3148..3162 /label=RBS-1\Strong RBS 3155..3159 /label=RBS misc_feature 3148..3162 /label=BBa_B0030 RBS 3148..3162 /label=BBa_B0030 CDS 3169..4749 /label=ATF1 misc_feature 4316..4316 /label=t -> c misc_feature 4744..4749 /label=double TAA stop codon misc_feature 3582..3582 /label=a->g to eliminate EcoRI site CDS 3169..4749 /label=BBa_J45014 stem_loop 4769..4812 /label=stem_loop misc_feature 4758..4837 /label=BBa_B0010 terminator 4758..4837 /label=BBa_B0010 stem_loop 4853..4872 /label=T7 TE misc_feature 4879..4879 /label=stop polyA_site 4873..4886 /label=polya misc_feature 4846..4886 /label=BBa_B0012 terminator 4846..4886 /label=BBa_B0012 ORIGIN 1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta 61 gagattaaag aggagaaata ctagatgacc ttggcacccc tagacgcctc caaagttaag 121 ataactacca cacaacatgc atctaagcca aaaccgaaca gtgagttagt gtttggcaag 181 agcttcacgg accacatgtt aactgcggaa tggacagctg aaaaagggtg gggtacccca 241 gagattaaac cttatcaaaa tctgtcttta gacccttccg cggtggtttt ccattatgct 301 tttgagctat tcgaagggat gaaggcttac agaacggtgg acaacaaaat tacaatgttt 361 cgtccagata tgaatatgaa gcgcatgaat aagtctgctc agagaatctg tttgccaacg 421 ttcgacccag aagagttgat taccctaatt gggaaactga tccagcaaga taagtgctta 481 gttcctgaag gaaaaggtta ctctttatat atcaggccta cattaatcgg cactacggcc 541 ggtttagggg tttccacgcc tgatagagcc ttgctatatg tcatttgctg ccctgtgggt 601 ccttattaca aaactggatt taaggcggtc agactggaag ccactgatta tgccacaaga 661 gcttggccag gaggctgtgg tgacaagaaa ctaggtgcaa actacgcccc ctgcgtcctg 721 ccacaattgc aagctgcttc aaggggttac caacaaaatt tatggctatt tggtccaaat 781 aacaacatta ctgaagtcgg caccatgaat gcttttttcg tgtttaaaga tagtaaaacg 841 ggcaagaagg aactggttac tgctccacta gacggtacca ttttggaagg tgttactagg 901 gattccattt taaatcttgc taaagaaaga ctcgaaccaa gtgaatggac cattagtgaa 961 cgctacttca ctataggcga agttactgag agatccaaga acggtgaact acttgaagcc 1021 tttggttctg gtactgctgc gattgtttct cccattaagg aaatcggctg gaaaggcgaa 1081 caaattaata ttccgttgtt gcccggcgaa caaaccggtc cattggccaa agaagttgca 1141 caatggatta atggaatcca atatggcgag actgagcatg gcaattggtc aagggttgtt 1201 actgatttga actaataata ctagagatta aagaggagaa atactagatg aattctagct 1261 atacacagag atatgcactg ccgaagtgta tagcaatatc agattatctt ttccatcggc 1321 tcaaccagct gaacatacat accatatttg gactctccgg agaatttagc atgccgttgc 1381 tggataaact atacaacatt ccgaacttac gatgggccgg taattctaat gagttaaatg 1441 ctgcctacgc agcagatgga tactcacgac taaaaggctt gggatgtctc ataacaacct 1501 ttggtgtagg cgaattatcg gcaatcaatg gcgtggccgg atcttacgct gaacatgtag 1561 gaatacttca catagtgggt atgccgccaa caagtgcaca aacgaaacaa ctactactgc 1621 atcatactct gggcaatggt gatttcacgg tatttcatag aatagccagt gatgtagcat 1681 gctatacaac attgattatt gactctgaat tatgtgccga cgaagtcgat aagtgcatca 1741 aaaaggcttg gatagaacag aggccagtat acatgggcat gcctgtcaac caggtaaatc 1801 tcccgattga atcagcaagg cttaatacac ctctggattt acaattgcat aaaaacgacc 1861 cagacgtaga gaaagaagtt atttctcgaa tattgagttt tatatacaaa agccagaatc 1921 cggcaatcat cgtagatgca tgtactagtc gacagaattt aatcgaggag actaaagagc 1981 tttgtaatag gcttaaattt ccagtttttg ttacacctat gggtaagggt acagtaaacg 2041 aaacagaccc gcaatttggg ggcgtattca cgggctcgat atcagcccca gaagtaagag 2101 aagtagttga ttttgccgat tttatcatcg tcattggttg catgctctcc gaattcagca 2161 cgtcaacttt ccacttccaa tataaaacta agaattgtgc gctactatat tctacatctg 2221 tgaaattgaa aaatgccaca tatcctgact tgagcattaa attactacta cagaaaatat 2281 tagcaaatct tgatgaatct aaactgtctt accaaccaag cgaacaaccc agtatgatgg 2341 ttccaagacc ttacccagca ggaaatgtcc tcttgagaca agaatgggtc tggaatgaaa 2401 tatcccattg gttccaacca ggtgacataa tcataacaga aactggtgct tctgcatttg 2461 gagttaacca gaccagattt ccggtaaata cactaggtat ttcgcaagct ctttggggat 2521 ctgtcggata tacaatgggg gcgtgtcttg gggcagaatt tgctgttcaa gagataaaca 2581 aggataaatt ccccgcaact aaacatagag ttattctgtt tatgggtgac ggtgctttcc 2641 aattgacagt tcaagaatta tccacaattg ttaagtgggg attgacacct tatatttttg 2701 tgatgaataa ccaaggttac tctgtggaca ggtttttgca tcacaggtca gatgctagtt 2761 attacgatat ccaaccttgg aactacttgg gattattgcg agtatttggt tgcacgaact 2821 acgaaacgaa aaaaattatt actgttggag aattcagatc catgatcagt gacccaaact 2881 ttgcgaccaa tgacaaaatt cggatgatag agattatgct accaccaagg gatgttccac 2941 aggctctgct tgacaggtgg gtggtagaaa aagaacagag caaacaagtg caagaggaga 3001 acgaaaattc tagcgcagta aatacgccaa ctccagaatt ccaaccactt ctaaaaaaaa 3061 atcaagttgg atactgatac tagagtccct atcagtgata gagattgaca tccctatcag 3121 tgatagagat actgagcact actagagatt aaagaggaga aatactagat gaatgaaatc 3181 gatgagaaaa atcaggcccc cgtgcaacaa gaatgcctga aagagatgat tcagaatggg 3241 catgctcggc gtatgggatc tgttgaagat ctgtatgttg ctctcaacag acaaaactta 3301 tatcgaaact tctgcacata tggagaattg agtgattact gtactaggga tcagctcaca 3361 ttagctttga gggaaatctg cctgaaaaat ccaactcttt tacatattgt tctaccaaca 3421 agatggccaa atcatgaaaa ttattatcgc agttccgaat actattcacg gccacatcca 3481 gtgcatgatt atatttcagt attacaagaa ttgaaactga gtggtgtggt tctcaatgaa 3541 caacctgagt acagtgcagt aatgaagcaa atattagaag agttcaaaaa tagtaagggt 3601 tcctatactg caaaaatttt taaacttact accactttga ctattcctta ctttggacca 3661 acaggaccga gttggcggct aatttgtctt ccagaagagc acacagaaaa gtggaaaaaa 3721 tttatctttg tatctaatca ttgcatgtct gatggtcggt cttcgatcca cttttttcat 3781 gatttaagag acgaattaaa taatattaaa actccaccaa aaaaattaga ttacattttc 3841 aagtacgagg aggattacca attattgagg aaacttccag aaccgatcga aaaggtgata 3901 gactttagac caccgtactt gtttattccg aagtcacttc tttcgggttt catctacaat 3961 catttgagat tttcttcaaa aggtgtctgt atgagaatgg atgatgtgga aaaaaccgat 4021 gatgttgtca ccgagatcat caatatttca ccaacagaat ttcaagcgat taaagcaaat 4081 attaaatcaa atatccaagg taagtgtact atcactccgt ttttacatgt ttgttggttt 4141 gtatctcttc ataaatgggg taaatttttc aaaccattga acttcgaatg gcttacggat 4201 atttttatcc ccgcagattg ccgctcacaa ctaccagatg atgatgaaat gagacagatg 4261 tacagatatg gcgctaacgt tggatttatt gacttcaccc cctggataag cgaatctgac 4321 atgaatgata acaaagaaaa tttttggcca cttattgagc actaccatga agtaatttcg 4381 gaagctttaa gaaataaaaa gcatctccat ggcttagggt tcaatataca aggcttcgtt 4441 caaaaatatg tgaacattga caaggtaatg tgcgatcgtg ccatcgggaa aagacgcgga 4501 ggtacattgt taagcaatgt aggtctgttt aatcagttag aggagcccga tgccaaatat 4561 tctatatgcg atttggcatt tggccaattt caaggatcct ggcaccaagc attttccttg 4621 ggtgtttgtt cgactaatgt aaaggggatg aatattgttg ttgcttcaac aaagaatgtt 4681 gttggtagtc aagaatctct cgaagagctt tgctccattt acaaagctct ccttttaggc 4741 ccttaataat actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc 4801 ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc 4861 ttcgggtggg cctttctgcg tttata //