LOCUS BBa_J45950 4886 bp DNA linear 22-Dec-2024 DEFINITION J45400.J45200 (const. banana) ACCESSION BBa_J45950 VERSION BBa_J45950.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 RBS 63..77 /label=RBS-1\Strong RBS 70..74 /label=RBS misc_feature 63..77 /label=BBa_B0030 RBS 63..77 /label=BBa_B0030 CDS 84..1664 /label=ATF1 misc_feature 1231..1231 /label=t -> c misc_feature 1659..1664 /label=double TAA stop codon misc_feature 497..497 /label=a->g to eliminate EcoRI site CDS 84..1664 /label=BBa_J45014 stem_loop 1684..1727 /label=stem_loop misc_feature 1673..1752 /label=BBa_B0010 terminator 1673..1752 /label=BBa_B0010 stem_loop 1768..1787 /label=T7 TE misc_feature 1794..1794 /label=stop polyA_site 1788..1801 /label=polya misc_feature 1761..1801 /label=BBa_B0012 terminator 1761..1801 /label=BBa_B0012 misc_feature 1812..1828 /label=lac O1 promoter 1829..1834 /label=-35 misc_feature 1835..1851 /label=lac O1 promoter 1852..1857 /label=-10 misc_feature 1810..1863 /label=BBa_R0011 promoter 1810..1863 /label=BBa_R0011 RBS 1873..1887 /label=RBS-1\Strong RBS 1880..1884 /label=RBS misc_feature 1873..1887 /label=BBa_B0030 RBS 1873..1887 /label=BBa_B0030 misc_feature 1894..1896 /label=start codon misc_feature 3022..3027 /label=double TAA stop codon misc_feature 2664..2664 /label=a->g to eliminate SpeI CDS 1894..3027 /label=BAT2 CDS 1894..3027 /label=BBa_J45008 RBS 3036..3050 /label=RBS-1\Strong RBS 3043..3047 /label=RBS misc_feature 3036..3050 /label=BBa_B0030 RBS 3036..3050 /label=BBa_B0030 misc_feature 3062..3062 /label=t->c to eliminate EcoRI, to do misc_feature 3962..3962 /label=a->g to eliminate EcoRI, to do misc_feature 4661..4661 /label=a->g to eliminate EcoRI, to do misc_feature 4847..4847 /label=a->g to eliminate EcoRI, to do misc_feature 3755..3755 /label=t->c to eliminate SpeI, to do CDS 3057..4886 /label=THI3 misc_feature 3057..3059 /label=start codon misc_feature 4884..4886 /label=stop codon CDS 3057..4886 /label=BBa_J45009 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 agattaaaga ggagaaatac tagatgaatg aaatcgatga gaaaaatcag gcccccgtgc 121 aacaagaatg cctgaaagag atgattcaga atgggcatgc tcggcgtatg ggatctgttg 181 aagatctgta tgttgctctc aacagacaaa acttatatcg aaacttctgc acatatggag 241 aattgagtga ttactgtact agggatcagc tcacattagc tttgagggaa atctgcctga 301 aaaatccaac tcttttacat attgttctac caacaagatg gccaaatcat gaaaattatt 361 atcgcagttc cgaatactat tcacggccac atccagtgca tgattatatt tcagtattac 421 aagaattgaa actgagtggt gtggttctca atgaacaacc tgagtacagt gcagtaatga 481 agcaaatatt agaagagttc aaaaatagta agggttccta tactgcaaaa atttttaaac 541 ttactaccac tttgactatt ccttactttg gaccaacagg accgagttgg cggctaattt 601 gtcttccaga agagcacaca gaaaagtgga aaaaatttat ctttgtatct aatcattgca 661 tgtctgatgg tcggtcttcg atccactttt ttcatgattt aagagacgaa ttaaataata 721 ttaaaactcc accaaaaaaa ttagattaca ttttcaagta cgaggaggat taccaattat 781 tgaggaaact tccagaaccg atcgaaaagg tgatagactt tagaccaccg tacttgttta 841 ttccgaagtc acttctttcg ggtttcatct acaatcattt gagattttct tcaaaaggtg 901 tctgtatgag aatggatgat gtggaaaaaa ccgatgatgt tgtcaccgag atcatcaata 961 tttcaccaac agaatttcaa gcgattaaag caaatattaa atcaaatatc caaggtaagt 1021 gtactatcac tccgttttta catgtttgtt ggtttgtatc tcttcataaa tggggtaaat 1081 ttttcaaacc attgaacttc gaatggctta cggatatttt tatccccgca gattgccgct 1141 cacaactacc agatgatgat gaaatgagac agatgtacag atatggcgct aacgttggat 1201 ttattgactt caccccctgg ataagcgaat ctgacatgaa tgataacaaa gaaaattttt 1261 ggccacttat tgagcactac catgaagtaa tttcggaagc tttaagaaat aaaaagcatc 1321 tccatggctt agggttcaat atacaaggct tcgttcaaaa atatgtgaac attgacaagg 1381 taatgtgcga tcgtgccatc gggaaaagac gcggaggtac attgttaagc aatgtaggtc 1441 tgtttaatca gttagaggag cccgatgcca aatattctat atgcgatttg gcatttggcc 1501 aatttcaagg atcctggcac caagcatttt ccttgggtgt ttgttcgact aatgtaaagg 1561 ggatgaatat tgttgttgct tcaacaaaga atgttgttgg tagtcaagaa tctctcgaag 1621 agctttgctc catttacaaa gctctccttt taggccctta ataatactag agccaggcat 1681 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg 1741 gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat 1801 atactagaga attgtgagcg gataacaatt gacattgtga gcggataaca agatactgag 1861 cacatactag agattaaaga ggagaaatac tagatgacct tggcacccct agacgcctcc 1921 aaagttaaga taactaccac acaacatgca tctaagccaa aaccgaacag tgagttagtg 1981 tttggcaaga gcttcacgga ccacatgtta actgcggaat ggacagctga aaaagggtgg 2041 ggtaccccag agattaaacc ttatcaaaat ctgtctttag acccttccgc ggtggttttc 2101 cattatgctt ttgagctatt cgaagggatg aaggcttaca gaacggtgga caacaaaatt 2161 acaatgtttc gtccagatat gaatatgaag cgcatgaata agtctgctca gagaatctgt 2221 ttgccaacgt tcgacccaga agagttgatt accctaattg ggaaactgat ccagcaagat 2281 aagtgcttag ttcctgaagg aaaaggttac tctttatata tcaggcctac attaatcggc 2341 actacggccg gtttaggggt ttccacgcct gatagagcct tgctatatgt catttgctgc 2401 cctgtgggtc cttattacaa aactggattt aaggcggtca gactggaagc cactgattat 2461 gccacaagag cttggccagg aggctgtggt gacaagaaac taggtgcaaa ctacgccccc 2521 tgcgtcctgc cacaattgca agctgcttca aggggttacc aacaaaattt atggctattt 2581 ggtccaaata acaacattac tgaagtcggc accatgaatg cttttttcgt gtttaaagat 2641 agtaaaacgg gcaagaagga actggttact gctccactag acggtaccat tttggaaggt 2701 gttactaggg attccatttt aaatcttgct aaagaaagac tcgaaccaag tgaatggacc 2761 attagtgaac gctacttcac tataggcgaa gttactgaga gatccaagaa cggtgaacta 2821 cttgaagcct ttggttctgg tactgctgcg attgtttctc ccattaagga aatcggctgg 2881 aaaggcgaac aaattaatat tccgttgttg cccggcgaac aaaccggtcc attggccaaa 2941 gaagttgcac aatggattaa tggaatccaa tatggcgaga ctgagcatgg caattggtca 3001 agggttgtta ctgatttgaa ctaataatac tagagattaa agaggagaaa tactagatga 3061 attctagcta tacacagaga tatgcactgc cgaagtgtat agcaatatca gattatcttt 3121 tccatcggct caaccagctg aacatacata ccatatttgg actctccgga gaatttagca 3181 tgccgttgct ggataaacta tacaacattc cgaacttacg atgggccggt aattctaatg 3241 agttaaatgc tgcctacgca gcagatggat actcacgact aaaaggcttg ggatgtctca 3301 taacaacctt tggtgtaggc gaattatcgg caatcaatgg cgtggccgga tcttacgctg 3361 aacatgtagg aatacttcac atagtgggta tgccgccaac aagtgcacaa acgaaacaac 3421 tactactgca tcatactctg ggcaatggtg atttcacggt atttcataga atagccagtg 3481 atgtagcatg ctatacaaca ttgattattg actctgaatt atgtgccgac gaagtcgata 3541 agtgcatcaa aaaggcttgg atagaacaga ggccagtata catgggcatg cctgtcaacc 3601 aggtaaatct cccgattgaa tcagcaaggc ttaatacacc tctggattta caattgcata 3661 aaaacgaccc agacgtagag aaagaagtta tttctcgaat attgagtttt atatacaaaa 3721 gccagaatcc ggcaatcatc gtagatgcat gtactagtcg acagaattta atcgaggaga 3781 ctaaagagct ttgtaatagg cttaaatttc cagtttttgt tacacctatg ggtaagggta 3841 cagtaaacga aacagacccg caatttgggg gcgtattcac gggctcgata tcagccccag 3901 aagtaagaga agtagttgat tttgccgatt ttatcatcgt cattggttgc atgctctccg 3961 aattcagcac gtcaactttc cacttccaat ataaaactaa gaattgtgcg ctactatatt 4021 ctacatctgt gaaattgaaa aatgccacat atcctgactt gagcattaaa ttactactac 4081 agaaaatatt agcaaatctt gatgaatcta aactgtctta ccaaccaagc gaacaaccca 4141 gtatgatggt tccaagacct tacccagcag gaaatgtcct cttgagacaa gaatgggtct 4201 ggaatgaaat atcccattgg ttccaaccag gtgacataat cataacagaa actggtgctt 4261 ctgcatttgg agttaaccag accagatttc cggtaaatac actaggtatt tcgcaagctc 4321 tttggggatc tgtcggatat acaatggggg cgtgtcttgg ggcagaattt gctgttcaag 4381 agataaacaa ggataaattc cccgcaacta aacatagagt tattctgttt atgggtgacg 4441 gtgctttcca attgacagtt caagaattat ccacaattgt taagtgggga ttgacacctt 4501 atatttttgt gatgaataac caaggttact ctgtggacag gtttttgcat cacaggtcag 4561 atgctagtta ttacgatatc caaccttgga actacttggg attattgcga gtatttggtt 4621 gcacgaacta cgaaacgaaa aaaattatta ctgttggaga attcagatcc atgatcagtg 4681 acccaaactt tgcgaccaat gacaaaattc ggatgataga gattatgcta ccaccaaggg 4741 atgttccaca ggctctgctt gacaggtggg tggtagaaaa agaacagagc aaacaagtgc 4801 aagaggagaa cgaaaattct agcgcagtaa atacgccaac tccagaattc caaccacttc 4861 taaaaaaaaa tcaagttgga tactga //