##gff-version 3
##sequence-region BBa_J45950 1 4886
BBa_J45950	.	terminator	1761	1801	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_J45950	.	engineered_region	1761	1801	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J45950	.	stem_loop	1768	1787	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J45950	.	polyA_site	1788	1801	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J45950	.	stop_codon	1794	1794	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J45950	.	ribosome_entry_site	63	77	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_J45950	.	engineered_region	63	77	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_J45950	.	ribosome_entry_site	70	74	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_J45950	.	ribosome_entry_site	63	77	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_J45950	.	CDS	3057	4886	.	+	0	ID=BBa_J45009;Name=BBa_J45009
BBa_J45950	.	CDS	3057	4886	.	+	0	ID=annotation1961298;Name=THI3;Parent=BBa_J45009
BBa_J45950	.	point_mutation	4661	4661	.	+	0	ID=annotation1891630;Name=a->g to eliminate EcoRI, to do;Parent=BBa_J45009
BBa_J45950	.	point_mutation	3755	3755	.	+	0	ID=annotation1891632;Name=t->c to eliminate SpeI, to do;Parent=BBa_J45009
BBa_J45950	.	point_mutation	3062	3062	.	+	0	ID=annotation1891628;Name=t->c to eliminate EcoRI, to do;Parent=BBa_J45009
BBa_J45950	.	point_mutation	3962	3962	.	+	0	ID=annotation1891629;Name=a->g to eliminate EcoRI, to do;Parent=BBa_J45009
BBa_J45950	.	start_codon	3057	3059	.	+	0	ID=annotation1961303;Name=start codon;Parent=BBa_J45009
BBa_J45950	.	point_mutation	4847	4847	.	+	0	ID=annotation1891631;Name=a->g to eliminate EcoRI, to do;Parent=BBa_J45009
BBa_J45950	.	stop_codon	4884	4886	.	+	0	ID=annotation1961304;Name=stop codon;Parent=BBa_J45009
BBa_J45950	.	ribosome_entry_site	3036	3050	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_J45950	.	engineered_region	3036	3050	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_J45950	.	ribosome_entry_site	3043	3047	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_J45950	.	ribosome_entry_site	3036	3050	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_J45950	.	CDS	1894	3027	.	+	0	ID=BBa_J45008;Name=BBa_J45008
BBa_J45950	.	CDS	1894	3027	.	+	0	ID=annotation1961299;Name=BAT2;Parent=BBa_J45008
BBa_J45950	.	point_mutation	2664	2664	.	+	0	ID=annotation1894553;Name=a->g to eliminate SpeI;Parent=BBa_J45008
BBa_J45950	.	start_codon	1894	1896	.	+	0	ID=annotation1883624;Name=start codon;Parent=BBa_J45008
BBa_J45950	.	stop_codon	3022	3027	.	+	0	ID=annotation1883625;Name=double TAA stop codon;Parent=BBa_J45008
BBa_J45950	.	CDS	84	1664	.	+	0	ID=BBa_J45014;Name=BBa_J45014
BBa_J45950	.	CDS	84	1664	.	+	0	ID=annotation1961300;Name=ATF1;Parent=BBa_J45014
BBa_J45950	.	point_mutation	497	497	.	+	0	ID=annotation1961305;Name=a->g to eliminate EcoRI site;Parent=BBa_J45014
BBa_J45950	.	stop_codon	1659	1664	.	+	0	ID=annotation1961302;Name=double TAA stop codon;Parent=BBa_J45014
BBa_J45950	.	sequence_alteration	1231	1231	.	+	0	ID=annotation1961301;Name=t -> c;Parent=BBa_J45014
BBa_J45950	.	promoter	1810	1863	.	+	0	ID=BBa_R0011;Name=BBa_R0011
BBa_J45950	.	operator	1812	1828	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_J45950	.	promoter	1852	1857	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_J45950	.	engineered_region	1810	1863	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_J45950	.	promoter	1829	1834	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_J45950	.	operator	1835	1851	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_J45950	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_J45950	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_J45950	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_J45950	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_J45950	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_J45950	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_J45950	.	ribosome_entry_site	1873	1887	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_J45950	.	engineered_region	1873	1887	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_J45950	.	ribosome_entry_site	1880	1884	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_J45950	.	ribosome_entry_site	1873	1887	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_J45950	.	terminator	1673	1752	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_J45950	.	engineered_region	1673	1752	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J45950	.	stem_loop	1684	1727	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
>BBa_J45950
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagattaaagaggagaaata
ctagatgaatgaaatcgatgagaaaaatcaggcccccgtgcaacaagaatgcctgaaagagatgattcagaatgggcat
gctcggcgtatgggatctgttgaagatctgtatgttgctctcaacagacaaaacttatatcgaaacttctgcacatatg
gagaattgagtgattactgtactagggatcagctcacattagctttgagggaaatctgcctgaaaaatccaactctttt
acatattgttctaccaacaagatggccaaatcatgaaaattattatcgcagttccgaatactattcacggccacatcca
gtgcatgattatatttcagtattacaagaattgaaactgagtggtgtggttctcaatgaacaacctgagtacagtgcag
taatgaagcaaatattagaagagttcaaaaatagtaagggttcctatactgcaaaaatttttaaacttactaccacttt
gactattccttactttggaccaacaggaccgagttggcggctaatttgtcttccagaagagcacacagaaaagtggaaa
aaatttatctttgtatctaatcattgcatgtctgatggtcggtcttcgatccacttttttcatgatttaagagacgaat
taaataatattaaaactccaccaaaaaaattagattacattttcaagtacgaggaggattaccaattattgaggaaact
tccagaaccgatcgaaaaggtgatagactttagaccaccgtacttgtttattccgaagtcacttctttcgggtttcatc
tacaatcatttgagattttcttcaaaaggtgtctgtatgagaatggatgatgtggaaaaaaccgatgatgttgtcaccg
agatcatcaatatttcaccaacagaatttcaagcgattaaagcaaatattaaatcaaatatccaaggtaagtgtactat
cactccgtttttacatgtttgttggtttgtatctcttcataaatggggtaaatttttcaaaccattgaacttcgaatgg
cttacggatatttttatccccgcagattgccgctcacaactaccagatgatgatgaaatgagacagatgtacagatatg
gcgctaacgttggatttattgacttcaccccctggataagcgaatctgacatgaatgataacaaagaaaatttttggcc
acttattgagcactaccatgaagtaatttcggaagctttaagaaataaaaagcatctccatggcttagggttcaatata
caaggcttcgttcaaaaatatgtgaacattgacaaggtaatgtgcgatcgtgccatcgggaaaagacgcggaggtacat
tgttaagcaatgtaggtctgtttaatcagttagaggagcccgatgccaaatattctatatgcgatttggcatttggcca
atttcaaggatcctggcaccaagcattttccttgggtgtttgttcgactaatgtaaaggggatgaatattgttgttgct
tcaacaaagaatgttgttggtagtcaagaatctctcgaagagctttgctccatttacaaagctctccttttaggccctt
aataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgt
cggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagaattgtga
gcggataacaattgacattgtgagcggataacaagatactgagcacatactagagattaaagaggagaaatactagatg
accttggcacccctagacgcctccaaagttaagataactaccacacaacatgcatctaagccaaaaccgaacagtgagt
tagtgtttggcaagagcttcacggaccacatgttaactgcggaatggacagctgaaaaagggtggggtaccccagagat
taaaccttatcaaaatctgtctttagacccttccgcggtggttttccattatgcttttgagctattcgaagggatgaag
gcttacagaacggtggacaacaaaattacaatgtttcgtccagatatgaatatgaagcgcatgaataagtctgctcaga
gaatctgtttgccaacgttcgacccagaagagttgattaccctaattgggaaactgatccagcaagataagtgcttagt
tcctgaaggaaaaggttactctttatatatcaggcctacattaatcggcactacggccggtttaggggtttccacgcct
gatagagccttgctatatgtcatttgctgccctgtgggtccttattacaaaactggatttaaggcggtcagactggaag
ccactgattatgccacaagagcttggccaggaggctgtggtgacaagaaactaggtgcaaactacgccccctgcgtcct
gccacaattgcaagctgcttcaaggggttaccaacaaaatttatggctatttggtccaaataacaacattactgaagtc
ggcaccatgaatgcttttttcgtgtttaaagatagtaaaacgggcaagaaggaactggttactgctccactagacggta
ccattttggaaggtgttactagggattccattttaaatcttgctaaagaaagactcgaaccaagtgaatggaccattag
tgaacgctacttcactataggcgaagttactgagagatccaagaacggtgaactacttgaagcctttggttctggtact
gctgcgattgtttctcccattaaggaaatcggctggaaaggcgaacaaattaatattccgttgttgcccggcgaacaaa
ccggtccattggccaaagaagttgcacaatggattaatggaatccaatatggcgagactgagcatggcaattggtcaag
ggttgttactgatttgaactaataatactagagattaaagaggagaaatactagatgaattctagctatacacagagat
atgcactgccgaagtgtatagcaatatcagattatcttttccatcggctcaaccagctgaacatacataccatatttgg
actctccggagaatttagcatgccgttgctggataaactatacaacattccgaacttacgatgggccggtaattctaat
gagttaaatgctgcctacgcagcagatggatactcacgactaaaaggcttgggatgtctcataacaacctttggtgtag
gcgaattatcggcaatcaatggcgtggccggatcttacgctgaacatgtaggaatacttcacatagtgggtatgccgcc
aacaagtgcacaaacgaaacaactactactgcatcatactctgggcaatggtgatttcacggtatttcatagaatagcc
agtgatgtagcatgctatacaacattgattattgactctgaattatgtgccgacgaagtcgataagtgcatcaaaaagg
cttggatagaacagaggccagtatacatgggcatgcctgtcaaccaggtaaatctcccgattgaatcagcaaggcttaa
tacacctctggatttacaattgcataaaaacgacccagacgtagagaaagaagttatttctcgaatattgagttttata
tacaaaagccagaatccggcaatcatcgtagatgcatgtactagtcgacagaatttaatcgaggagactaaagagcttt
gtaataggcttaaatttccagtttttgttacacctatgggtaagggtacagtaaacgaaacagacccgcaatttggggg
cgtattcacgggctcgatatcagccccagaagtaagagaagtagttgattttgccgattttatcatcgtcattggttgc
atgctctccgaattcagcacgtcaactttccacttccaatataaaactaagaattgtgcgctactatattctacatctg
tgaaattgaaaaatgccacatatcctgacttgagcattaaattactactacagaaaatattagcaaatcttgatgaatc
taaactgtcttaccaaccaagcgaacaacccagtatgatggttccaagaccttacccagcaggaaatgtcctcttgaga
caagaatgggtctggaatgaaatatcccattggttccaaccaggtgacataatcataacagaaactggtgcttctgcat
ttggagttaaccagaccagatttccggtaaatacactaggtatttcgcaagctctttggggatctgtcggatatacaat
gggggcgtgtcttggggcagaatttgctgttcaagagataaacaaggataaattccccgcaactaaacatagagttatt
ctgtttatgggtgacggtgctttccaattgacagttcaagaattatccacaattgttaagtggggattgacaccttata
tttttgtgatgaataaccaaggttactctgtggacaggtttttgcatcacaggtcagatgctagttattacgatatcca
accttggaactacttgggattattgcgagtatttggttgcacgaactacgaaacgaaaaaaattattactgttggagaa
ttcagatccatgatcagtgacccaaactttgcgaccaatgacaaaattcggatgatagagattatgctaccaccaaggg
atgttccacaggctctgcttgacaggtgggtggtagaaaaagaacagagcaaacaagtgcaagaggagaacgaaaattc
tagcgcagtaaatacgccaactccagaattccaaccacttctaaaaaaaaatcaagttggatactga