LOCUS BBa_J45990 2856 bp DNA linear 22-May-2024 DEFINITION Exponential phase expression of ATFI ACCESSION BBa_J45990 VERSION BBa_J45990.1 FEATURES Location/Qualifiers promoter 188..193 /label=-10 promoter 164..169 /label=-35 promoter 1..199 /label=BBa_J45992 RBS 208..219 /label=BBa_B0064 CDS 229..845 /label=tetR misc_feature 846..879 /label=SsrA misc_feature 886..910 /label=Help:Barcodes CDS 226..910 /label=BBa_C0040 stem_loop 930..973 /label=stem_loop misc_feature 919..998 /label=BBa_B0010 terminator 919..998 /label=BBa_B0010 stem_loop 1014..1033 /label=T7 TE misc_feature 1040..1040 /label=stop polyA_site 1034..1047 /label=polya misc_feature 1007..1047 /label=BBa_B0012 terminator 1007..1047 /label=BBa_B0012 terminator 919..1047 /label=BBa_B0015 misc_feature 1056..1074 /label=TetR 1 misc_feature 1056..1109 /label=BBa_R0040 promoter 1075..1080 /label=-35 misc_feature 1081..1099 /label=TetR 2 promoter 1098..1103 /label=-10 promoter 1056..1109 /label=BBa_R0040 misc_feature 208..1109 /label=BBa_Q04401 RBS 1118..1132 /label=RBS-1\Strong RBS 1125..1129 /label=RBS misc_feature 1118..1132 /label=BBa_B0030 RBS 1118..1132 /label=BBa_B0030 CDS 1139..2719 /label=ATF1 misc_feature 2286..2286 /label=t -> c misc_feature 2714..2719 /label=double TAA stop codon misc_feature 1552..1552 /label=a->g to eliminate EcoRI site CDS 1139..2719 /label=BBa_J45014 stem_loop 2739..2782 /label=stem_loop misc_feature 2728..2807 /label=BBa_B0010 terminator 2728..2807 /label=BBa_B0010 stem_loop 2823..2842 /label=T7 TE misc_feature 2849..2849 /label=stop polyA_site 2843..2856 /label=polya misc_feature 2816..2856 /label=BBa_B0012 terminator 2816..2856 /label=BBa_B0012 misc_feature 1118..2856 /label=BBa_J45199 ORIGIN 1 ctggcacagg aacgttatcc ggacgttcag ttccaccaga cccgcgagca ttaattcttg 61 cctccagggc gcggtagtgg cgccctgtca atttcccttc cttattagcc gcttacggaa 121 tgttcttaaa acattcactt ttgcttatgt tttcgctgat atcccgagcg gtttcaaaat 181 tgtgatctat atttaacaat actagagaaa gaggggaaat actagatgtc cagattagat 241 aaaagtaaag tgattaacag cgcattagag ctgcttaatg aggtcggaat cgaaggttta 301 acaacccgta aactcgccca gaagctaggt gtagagcagc ctacattgta ttggcatgta 361 aaaaataagc gggctttgct cgacgcctta gccattgaga tgttagatag gcaccatact 421 cacttttgcc ctttagaagg ggaaagctgg caagattttt tacgtaataa cgctaaaagt 481 tttagatgtg ctttactaag tcatcgcgat ggagcaaaag tacatttagg tacacggcct 541 acagaaaaac agtatgaaac tctcgaaaat caattagcct ttttatgcca acaaggtttt 601 tcactagaga atgcattata tgcactcagc gctgtggggc attttacttt aggttgcgta 661 ttggaagatc aagagcatca agtcgctaaa gaagaaaggg aaacacctac tactgatagt 721 atgccgccat tattacgaca agctatcgaa ttatttgatc accaaggtgc agagccagcc 781 ttcttattcg gccttgaatt gatcatatgc ggattagaaa aacaacttaa atgtgaaagt 841 gggtccgctg caaacgacga aaactacgct ttagtagctt aataacactg atagtgctag 901 tgtagatcac tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg 961 cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac 1021 cttcgggtgg gcctttctgc gtttatatac tagagtccct atcagtgata gagattgaca 1081 tccctatcag tgatagagat actgagcact actagagatt aaagaggaga aatactagat 1141 gaatgaaatc gatgagaaaa atcaggcccc cgtgcaacaa gaatgcctga aagagatgat 1201 tcagaatggg catgctcggc gtatgggatc tgttgaagat ctgtatgttg ctctcaacag 1261 acaaaactta tatcgaaact tctgcacata tggagaattg agtgattact gtactaggga 1321 tcagctcaca ttagctttga gggaaatctg cctgaaaaat ccaactcttt tacatattgt 1381 tctaccaaca agatggccaa atcatgaaaa ttattatcgc agttccgaat actattcacg 1441 gccacatcca gtgcatgatt atatttcagt attacaagaa ttgaaactga gtggtgtggt 1501 tctcaatgaa caacctgagt acagtgcagt aatgaagcaa atattagaag agttcaaaaa 1561 tagtaagggt tcctatactg caaaaatttt taaacttact accactttga ctattcctta 1621 ctttggacca acaggaccga gttggcggct aatttgtctt ccagaagagc acacagaaaa 1681 gtggaaaaaa tttatctttg tatctaatca ttgcatgtct gatggtcggt cttcgatcca 1741 cttttttcat gatttaagag acgaattaaa taatattaaa actccaccaa aaaaattaga 1801 ttacattttc aagtacgagg aggattacca attattgagg aaacttccag aaccgatcga 1861 aaaggtgata gactttagac caccgtactt gtttattccg aagtcacttc tttcgggttt 1921 catctacaat catttgagat tttcttcaaa aggtgtctgt atgagaatgg atgatgtgga 1981 aaaaaccgat gatgttgtca ccgagatcat caatatttca ccaacagaat ttcaagcgat 2041 taaagcaaat attaaatcaa atatccaagg taagtgtact atcactccgt ttttacatgt 2101 ttgttggttt gtatctcttc ataaatgggg taaatttttc aaaccattga acttcgaatg 2161 gcttacggat atttttatcc ccgcagattg ccgctcacaa ctaccagatg atgatgaaat 2221 gagacagatg tacagatatg gcgctaacgt tggatttatt gacttcaccc cctggataag 2281 cgaatctgac atgaatgata acaaagaaaa tttttggcca cttattgagc actaccatga 2341 agtaatttcg gaagctttaa gaaataaaaa gcatctccat ggcttagggt tcaatataca 2401 aggcttcgtt caaaaatatg tgaacattga caaggtaatg tgcgatcgtg ccatcgggaa 2461 aagacgcgga ggtacattgt taagcaatgt aggtctgttt aatcagttag aggagcccga 2521 tgccaaatat tctatatgcg atttggcatt tggccaattt caaggatcct ggcaccaagc 2581 attttccttg ggtgtttgtt cgactaatgt aaaggggatg aatattgttg ttgcttcaac 2641 aaagaatgtt gttggtagtc aagaatctct cgaagagctt tgctccattt acaaagctct 2701 ccttttaggc ccttaataat actagagcca ggcatcaaat aaaacgaaag gctcagtcga 2761 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca 2821 ctggctcacc ttcgggtggg cctttctgcg tttata //