##gff-version 3
##sequence-region BBa_J45990 1 2856
BBa_J45990	.	promoter	1	199	.	+	0	ID=BBa_J45992;Name=BBa_J45992
BBa_J45990	.	promoter	188	193	.	+	0	ID=annotation1883645;Name=-10;Parent=BBa_J45992
BBa_J45990	.	promoter	164	169	.	+	0	ID=annotation1883646;Name=-35;Parent=BBa_J45992
BBa_J45990	.	engineered_region	1118	2856	.	+	0	ID=BBa_J45199;Name=BBa_J45199
BBa_J45990	.	ribosome_entry_site	1118	1132	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_J45199
BBa_J45990	.	ribosome_entry_site	1125	1129	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_J45990	.	engineered_region	1118	1132	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_J45990	.	ribosome_entry_site	1118	1132	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_J45990	.	terminator	2728	2807	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J45199
BBa_J45990	.	stem_loop	2739	2782	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J45990	.	engineered_region	2728	2807	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J45990	.	terminator	2816	2856	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J45199
BBa_J45990	.	stem_loop	2823	2842	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J45990	.	polyA_site	2843	2856	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J45990	.	stop_codon	2849	2849	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J45990	.	engineered_region	2816	2856	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J45990	.	CDS	1139	2719	.	+	0	ID=BBa_J45014;Name=BBa_J45014;Parent=BBa_J45199
BBa_J45990	.	sequence_alteration	2286	2286	.	+	0	ID=annotation1961301;Name=t -> c;Parent=BBa_J45014
BBa_J45990	.	CDS	1139	2719	.	+	0	ID=annotation1961300;Name=ATF1;Parent=BBa_J45014
BBa_J45990	.	stop_codon	2714	2719	.	+	0	ID=annotation1961302;Name=double TAA stop codon;Parent=BBa_J45014
BBa_J45990	.	point_mutation	1552	1552	.	+	0	ID=annotation1961305;Name=a->g to eliminate EcoRI site;Parent=BBa_J45014
BBa_J45990	.	engineered_region	208	1109	.	+	0	ID=BBa_Q04401;Name=BBa_Q04401
BBa_J45990	.	ribosome_entry_site	208	219	.	+	0	ID=BBa_B0064;Name=BBa_B0064;Parent=BBa_Q04401
BBa_J45990	.	promoter	1056	1109	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_Q04401
BBa_J45990	.	non_covalent_binding_site	1056	1074	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_J45990	.	engineered_region	1056	1109	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_J45990	.	promoter	1075	1080	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_J45990	.	non_covalent_binding_site	1081	1099	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_J45990	.	promoter	1098	1103	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_J45990	.	CDS	226	910	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_Q04401
BBa_J45990	.	engineered_tag	886	910	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_J45990	.	CDS	229	845	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_J45990	.	tag	846	879	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_J45990	.	terminator	919	1047	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_Q04401
BBa_J45990	.	terminator	1007	1047	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_J45990	.	stem_loop	1014	1033	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J45990	.	polyA_site	1034	1047	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J45990	.	stop_codon	1040	1040	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J45990	.	engineered_region	1007	1047	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J45990	.	terminator	919	998	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_J45990	.	stem_loop	930	973	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J45990	.	engineered_region	919	998	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
>BBa_J45990
ctggcacaggaacgttatccggacgttcagttccaccagacccgcgagcattaattcttgcctccagggcgcggtagtg
gcgccctgtcaatttcccttccttattagccgcttacggaatgttcttaaaacattcacttttgcttatgttttcgctg
atatcccgagcggtttcaaaattgtgatctatatttaacaatactagagaaagaggggaaatactagatgtccagatta
gataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcg
cccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccat
tgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgct
aaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagt
atgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgc
tgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctact
actgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcg
gccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgc
tttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagt
cgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcggg
tgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgag
cactactagagattaaagaggagaaatactagatgaatgaaatcgatgagaaaaatcaggcccccgtgcaacaagaatg
cctgaaagagatgattcagaatgggcatgctcggcgtatgggatctgttgaagatctgtatgttgctctcaacagacaa
aacttatatcgaaacttctgcacatatggagaattgagtgattactgtactagggatcagctcacattagctttgaggg
aaatctgcctgaaaaatccaactcttttacatattgttctaccaacaagatggccaaatcatgaaaattattatcgcag
ttccgaatactattcacggccacatccagtgcatgattatatttcagtattacaagaattgaaactgagtggtgtggtt
ctcaatgaacaacctgagtacagtgcagtaatgaagcaaatattagaagagttcaaaaatagtaagggttcctatactg
caaaaatttttaaacttactaccactttgactattccttactttggaccaacaggaccgagttggcggctaatttgtct
tccagaagagcacacagaaaagtggaaaaaatttatctttgtatctaatcattgcatgtctgatggtcggtcttcgatc
cacttttttcatgatttaagagacgaattaaataatattaaaactccaccaaaaaaattagattacattttcaagtacg
aggaggattaccaattattgaggaaacttccagaaccgatcgaaaaggtgatagactttagaccaccgtacttgtttat
tccgaagtcacttctttcgggtttcatctacaatcatttgagattttcttcaaaaggtgtctgtatgagaatggatgat
gtggaaaaaaccgatgatgttgtcaccgagatcatcaatatttcaccaacagaatttcaagcgattaaagcaaatatta
aatcaaatatccaaggtaagtgtactatcactccgtttttacatgtttgttggtttgtatctcttcataaatggggtaa
atttttcaaaccattgaacttcgaatggcttacggatatttttatccccgcagattgccgctcacaactaccagatgat
gatgaaatgagacagatgtacagatatggcgctaacgttggatttattgacttcaccccctggataagcgaatctgaca
tgaatgataacaaagaaaatttttggccacttattgagcactaccatgaagtaatttcggaagctttaagaaataaaaa
gcatctccatggcttagggttcaatatacaaggcttcgttcaaaaatatgtgaacattgacaaggtaatgtgcgatcgt
gccatcgggaaaagacgcggaggtacattgttaagcaatgtaggtctgtttaatcagttagaggagcccgatgccaaat
attctatatgcgatttggcatttggccaatttcaaggatcctggcaccaagcattttccttgggtgtttgttcgactaa
tgtaaaggggatgaatattgttgttgcttcaacaaagaatgttgttggtagtcaagaatctctcgaagagctttgctcc
atttacaaagctctccttttaggcccttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagac
tgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctt
tctgcgtttata