LOCUS       BBa_J45996              1995 bp    DNA     linear       19-Dec-2024
DEFINITION  Exponential phase dependent GFP generator
ACCESSION   BBa_J45996
VERSION     BBa_J45996.1  
FEATURES             Location/Qualifiers
     promoter        188..193
                     /label=-10
     promoter        164..169
                     /label=-35
     promoter        1..199
                     /label=BBa_J45992
     RBS             208..219
                     /label=BBa_B0064
     CDS             229..845
                     /label=tetR
     misc_feature    846..879
                     /label=SsrA
     misc_feature    886..910
                     /label=Help:Barcodes
     CDS             226..910
                     /label=BBa_C0040
     stem_loop       930..973
                     /label=stem_loop
     misc_feature    919..998
                     /label=BBa_B0010
     terminator      919..998
                     /label=BBa_B0010
     stem_loop       1014..1033
                     /label=T7 TE
     misc_feature    1040..1040
                     /label=stop
     polyA_site      1034..1047
                     /label=polya
     misc_feature    1007..1047
                     /label=BBa_B0012
     terminator      1007..1047
                     /label=BBa_B0012
     terminator      919..1047
                     /label=BBa_B0015
     misc_feature    1056..1074
                     /label=TetR 1
     misc_feature    1056..1109
                     /label=BBa_R0040
     promoter        1075..1080
                     /label=-35
     misc_feature    1081..1099
                     /label=TetR 2
     promoter        1098..1103
                     /label=-10
     promoter        1056..1109
                     /label=BBa_R0040
     misc_feature    208..1109
                     /label=BBa_Q04401
     RBS             1118..1132
                     /label=RBS-1\Strong
     RBS             1125..1129
                     /label=RBS
     misc_feature    1118..1132
                     /label=BBa_B0030
     RBS             1118..1132
                     /label=BBa_B0030
     CDS             1139..1858
                     /label=GFP protein
     CDS             1139..1858
                     /label=BBa_E0040
     stem_loop       1878..1921
                     /label=stem_loop
     misc_feature    1867..1946
                     /label=BBa_B0010
     terminator      1867..1946
                     /label=BBa_B0010
     stem_loop       1962..1981
                     /label=T7 TE
     misc_feature    1988..1988
                     /label=stop
     polyA_site      1982..1995
                     /label=polya
     misc_feature    1955..1995
                     /label=BBa_B0012
     terminator      1955..1995
                     /label=BBa_B0012
ORIGIN
        1 ctggcacagg aacgttatcc ggacgttcag ttccaccaga cccgcgagca ttaattcttg
       61 cctccagggc gcggtagtgg cgccctgtca atttcccttc cttattagcc gcttacggaa
      121 tgttcttaaa acattcactt ttgcttatgt tttcgctgat atcccgagcg gtttcaaaat
      181 tgtgatctat atttaacaat actagagaaa gaggggaaat actagatgtc cagattagat
      241 aaaagtaaag tgattaacag cgcattagag ctgcttaatg aggtcggaat cgaaggttta
      301 acaacccgta aactcgccca gaagctaggt gtagagcagc ctacattgta ttggcatgta
      361 aaaaataagc gggctttgct cgacgcctta gccattgaga tgttagatag gcaccatact
      421 cacttttgcc ctttagaagg ggaaagctgg caagattttt tacgtaataa cgctaaaagt
      481 tttagatgtg ctttactaag tcatcgcgat ggagcaaaag tacatttagg tacacggcct
      541 acagaaaaac agtatgaaac tctcgaaaat caattagcct ttttatgcca acaaggtttt
      601 tcactagaga atgcattata tgcactcagc gctgtggggc attttacttt aggttgcgta
      661 ttggaagatc aagagcatca agtcgctaaa gaagaaaggg aaacacctac tactgatagt
      721 atgccgccat tattacgaca agctatcgaa ttatttgatc accaaggtgc agagccagcc
      781 ttcttattcg gccttgaatt gatcatatgc ggattagaaa aacaacttaa atgtgaaagt
      841 gggtccgctg caaacgacga aaactacgct ttagtagctt aataacactg atagtgctag
      901 tgtagatcac tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg
      961 cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac
     1021 cttcgggtgg gcctttctgc gtttatatac tagagtccct atcagtgata gagattgaca
     1081 tccctatcag tgatagagat actgagcact actagagatt aaagaggaga aatactagat
     1141 gcgtaaagga gaagaacttt tcactggagt tgtcccaatt cttgttgaat tagatggtga
     1201 tgttaatggg cacaaatttt ctgtcagtgg agagggtgaa ggtgatgcaa catacggaaa
     1261 acttaccctt aaatttattt gcactactgg aaaactacct gttccatggc caacacttgt
     1321 cactactttc ggttatggtg ttcaatgctt tgcgagatac ccagatcata tgaaacagca
     1381 tgactttttc aagagtgcca tgcccgaagg ttatgtacag gaaagaacta tatttttcaa
     1441 agatgacggg aactacaaga cacgtgctga agtcaagttt gaaggtgata cccttgttaa
     1501 tagaatcgag ttaaaaggta ttgattttaa agaagatgga aacattcttg gacacaaatt
     1561 ggaatacaac tataactcac acaatgtata catcatggca gacaaacaaa agaatggaat
     1621 caaagttaac ttcaaaatta gacacaacat tgaagatgga agcgttcaac tagcagacca
     1681 ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt ttaccagaca accattacct
     1741 gtccacacaa tctgcccttt cgaaagatcc caacgaaaag agagaccaca tggtccttct
     1801 tgagtttgta acagctgctg ggattacaca tggcatggat gaactataca aataataata
     1861 ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta
     1921 tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc
     1981 ctttctgcgt ttata
//