LOCUS       BBa_J45998              1853 bp    DNA     linear       12-May-2024
DEFINITION  Exponential Phase Activated GFP Generator with the Truncated osmY 
            Promoter
ACCESSION   BBa_J45998
VERSION     BBa_J45998.1  
FEATURES             Location/Qualifiers
     promoter        46..51
                     /label=-10
     promoter        22..27
                     /label=-35
     promoter        1..57
                     /label=BBa_J45993
     RBS             66..77
                     /label=BBa_B0064
     CDS             87..703
                     /label=tetR
     misc_feature    704..737
                     /label=SsrA
     misc_feature    744..768
                     /label=Help:Barcodes
     CDS             84..768
                     /label=BBa_C0040
     stem_loop       788..831
                     /label=stem_loop
     misc_feature    777..856
                     /label=BBa_B0010
     terminator      777..856
                     /label=BBa_B0010
     stem_loop       872..891
                     /label=T7 TE
     misc_feature    898..898
                     /label=stop
     polyA_site      892..905
                     /label=polya
     misc_feature    865..905
                     /label=BBa_B0012
     terminator      865..905
                     /label=BBa_B0012
     terminator      777..905
                     /label=BBa_B0015
     misc_feature    914..932
                     /label=TetR 1
     misc_feature    914..967
                     /label=BBa_R0040
     promoter        933..938
                     /label=-35
     misc_feature    939..957
                     /label=TetR 2
     promoter        956..961
                     /label=-10
     promoter        914..967
                     /label=BBa_R0040
     misc_feature    66..967
                     /label=BBa_Q04401
     RBS             976..990
                     /label=RBS-1\Strong
     RBS             983..987
                     /label=RBS
     misc_feature    976..990
                     /label=BBa_B0030
     RBS             976..990
                     /label=BBa_B0030
     CDS             997..1716
                     /label=GFP protein
     CDS             997..1716
                     /label=BBa_E0040
     stem_loop       1736..1779
                     /label=stem_loop
     misc_feature    1725..1804
                     /label=BBa_B0010
     terminator      1725..1804
                     /label=BBa_B0010
     stem_loop       1820..1839
                     /label=T7 TE
     misc_feature    1846..1846
                     /label=stop
     polyA_site      1840..1853
                     /label=polya
     misc_feature    1813..1853
                     /label=BBa_B0012
     terminator      1813..1853
                     /label=BBa_B0012
ORIGIN
        1 gcttatgttt tcgctgatat cccgagcggt ttcaaaattg tgatctatat ttaacaatac
       61 tagagaaaga ggggaaatac tagatgtcca gattagataa aagtaaagtg attaacagcg
      121 cattagagct gcttaatgag gtcggaatcg aaggtttaac aacccgtaaa ctcgcccaga
      181 agctaggtgt agagcagcct acattgtatt ggcatgtaaa aaataagcgg gctttgctcg
      241 acgccttagc cattgagatg ttagataggc accatactca cttttgccct ttagaagggg
      301 aaagctggca agatttttta cgtaataacg ctaaaagttt tagatgtgct ttactaagtc
      361 atcgcgatgg agcaaaagta catttaggta cacggcctac agaaaaacag tatgaaactc
      421 tcgaaaatca attagccttt ttatgccaac aaggtttttc actagagaat gcattatatg
      481 cactcagcgc tgtggggcat tttactttag gttgcgtatt ggaagatcaa gagcatcaag
      541 tcgctaaaga agaaagggaa acacctacta ctgatagtat gccgccatta ttacgacaag
      601 ctatcgaatt atttgatcac caaggtgcag agccagcctt cttattcggc cttgaattga
      661 tcatatgcgg attagaaaaa caacttaaat gtgaaagtgg gtccgctgca aacgacgaaa
      721 actacgcttt agtagcttaa taacactgat agtgctagtg tagatcacta ctagagccag
      781 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt
      841 gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt
      901 ttatatacta gagtccctat cagtgataga gattgacatc cctatcagtg atagagatac
      961 tgagcactac tagagattaa agaggagaaa tactagatgc gtaaaggaga agaacttttc
     1021 actggagttg tcccaattct tgttgaatta gatggtgatg ttaatgggca caaattttct
     1081 gtcagtggag agggtgaagg tgatgcaaca tacggaaaac ttacccttaa atttatttgc
     1141 actactggaa aactacctgt tccatggcca acacttgtca ctactttcgg ttatggtgtt
     1201 caatgctttg cgagataccc agatcatatg aaacagcatg actttttcaa gagtgccatg
     1261 cccgaaggtt atgtacagga aagaactata tttttcaaag atgacgggaa ctacaagaca
     1321 cgtgctgaag tcaagtttga aggtgatacc cttgttaata gaatcgagtt aaaaggtatt
     1381 gattttaaag aagatggaaa cattcttgga cacaaattgg aatacaacta taactcacac
     1441 aatgtataca tcatggcaga caaacaaaag aatggaatca aagttaactt caaaattaga
     1501 cacaacattg aagatggaag cgttcaacta gcagaccatt atcaacaaaa tactccaatt
     1561 ggcgatggcc ctgtcctttt accagacaac cattacctgt ccacacaatc tgccctttcg
     1621 aaagatccca acgaaaagag agaccacatg gtccttcttg agtttgtaac agctgctggg
     1681 attacacatg gcatggatga actatacaaa taataatact agagccaggc atcaaataaa
     1741 acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc
     1801 tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt ata
//