##gff-version 3
##sequence-region BBa_J45998 1 1853
BBa_J45998	.	promoter	1	57	.	+	0	ID=BBa_J45993;Name=BBa_J45993
BBa_J45998	.	promoter	22	27	.	+	0	ID=annotation1883648;Name=-35;Parent=BBa_J45993
BBa_J45998	.	promoter	46	51	.	+	0	ID=annotation1883647;Name=-10;Parent=BBa_J45993
BBa_J45998	.	ribosome_entry_site	976	990	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_J45998	.	engineered_region	976	990	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_J45998	.	ribosome_entry_site	983	987	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_J45998	.	ribosome_entry_site	976	990	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_J45998	.	terminator	1725	1804	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_J45998	.	stem_loop	1736	1779	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J45998	.	engineered_region	1725	1804	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J45998	.	CDS	997	1716	.	+	0	ID=BBa_E0040;Name=BBa_E0040
BBa_J45998	.	CDS	997	1716	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_J45998	.	engineered_region	66	967	.	+	0	ID=BBa_Q04401;Name=BBa_Q04401
BBa_J45998	.	CDS	84	768	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_Q04401
BBa_J45998	.	engineered_tag	744	768	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_J45998	.	tag	704	737	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_J45998	.	CDS	87	703	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_J45998	.	promoter	914	967	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_Q04401
BBa_J45998	.	promoter	933	938	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_J45998	.	non_covalent_binding_site	939	957	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_J45998	.	engineered_region	914	967	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_J45998	.	non_covalent_binding_site	914	932	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_J45998	.	promoter	956	961	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_J45998	.	terminator	777	905	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_Q04401
BBa_J45998	.	terminator	777	856	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_J45998	.	stem_loop	788	831	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J45998	.	engineered_region	777	856	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J45998	.	terminator	865	905	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_J45998	.	engineered_region	865	905	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J45998	.	stop_codon	898	898	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J45998	.	stem_loop	872	891	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J45998	.	polyA_site	892	905	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J45998	.	ribosome_entry_site	66	77	.	+	0	ID=BBa_B0064;Name=BBa_B0064;Parent=BBa_Q04401
BBa_J45998	.	terminator	1813	1853	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_J45998	.	engineered_region	1813	1853	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J45998	.	stop_codon	1846	1846	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J45998	.	stem_loop	1820	1839	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J45998	.	polyA_site	1840	1853	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
>BBa_J45998
gcttatgttttcgctgatatcccgagcggtttcaaaattgtgatctatatttaacaatactagagaaagaggggaaata
ctagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggttta
acaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgc
tcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattt
tttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacgg
cctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcat
tatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaaga
aagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagag
ccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaa
acgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaata
aaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcaca
ctggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcag
tgatagagatactgagcactactagagattaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggag
ttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgc
aacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactact
ttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccg
aaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaagg
tgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaa
tacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagac
acaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtcctttt
accagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttctt
gagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaata
aaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcaca
ctggctcaccttcgggtgggcctttctgcgtttata