LOCUS       BBa_J46001              2617 bp    DNA     linear       25-Nov-2024
DEFINITION  HSL amplifier
ACCESSION   BBa_J46001
VERSION     BBa_J46001.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     misc_feature    81..82
                     /label=prefix
     misc_feature    254..254
                     /label=T
     misc_feature    572..572
                     /label=A
     CDS             81..830
                     /label=luxR
     misc_feature    81..836
                     /label=BBa_C0062
     misc_feature    837..861
                     /label=Help:Barcodes
     CDS             81..836
                     /label=BBa_C0062
     stem_loop       881..924
                     /label=stem_loop
     misc_feature    870..949
                     /label=BBa_B0010
     terminator      870..949
                     /label=BBa_B0010
     stem_loop       965..984
                     /label=T7 TE
     misc_feature    991..991
                     /label=stop
     polyA_site      985..998
                     /label=polya
     misc_feature    958..998
                     /label=BBa_B0012
     terminator      958..998
                     /label=BBa_B0012
     terminator      870..998
                     /label=BBa_B0015
     misc_feature    1007..1026
                     /label=LuxR/HSL
     promoter        1026..1031
                     /label=-35
     promoter        1048..1053
                     /label=-10
     misc_feature    1059..1059
                     /label=start
     misc_feature    1007..1061
                     /label=BBa_R0062
     promoter        1007..1061
                     /label=BBa_R0062
     misc_feature    1..1061
                     /label=BBa_F2620
     RBS             1070..1082
                     /label=RBS-3\Weak
     RBS             1076..1079
                     /label=RBS
     misc_feature    1070..1082
                     /label=BBa_B0032
     RBS             1070..1082
                     /label=BBa_B0032
     CDS             1089..1811
                     /label=BBa_E0030
     misc_feature    1070..1811
                     /label=BBa_I15017
     misc_feature    1824..1827
                     /label=conserved
     RBS             1820..1831
                     /label=BBa_B0034
     misc_feature    2417..2448
                     /label=LVA
     CDS             1838..2416
                     /label=luxI
     misc_feature    1838..2455
                     /label=BBa_C0061
     misc_feature    2456..2480
                     /label=Help:Barcodes
     CDS             1838..2455
                     /label=BBa_C0061
     stem_loop       2500..2543
                     /label=stem_loop
     misc_feature    2489..2568
                     /label=BBa_B0010
     terminator      2489..2568
                     /label=BBa_B0010
     stem_loop       2584..2603
                     /label=T7 TE
     misc_feature    2610..2610
                     /label=stop
     polyA_site      2604..2617
                     /label=polya
     misc_feature    2577..2617
                     /label=BBa_B0012
     terminator      2577..2617
                     /label=BBa_B0012
     misc_feature    1820..2617
                     /label=BBa_F1610
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgaaaaaca taaatgccga cgacacatac agaataatta
      121 ataaaattaa agcttgtaga agcaataatg atattaatca atgcttatct gatatgacta
      181 aaatggtaca ttgtgaatat tatttactcg cgatcattta tcctcattct atggttaaat
      241 ctgatatttc aatcctagat aattacccta aaaaatggag gcaatattat gatgacgcta
      301 atttaataaa atatgatcct atagtagatt attctaactc caatcattca ccaattaatt
      361 ggaatatatt tgaaaacaat gctgtaaata aaaaatctcc aaatgtaatt aaagaagcga
      421 aaacatcagg tcttatcact gggtttagtt tccctattca tacggctaac aatggcttcg
      481 gaatgcttag ttttgcacat tcagaaaaag acaactatat agatagttta tttttacatg
      541 cgtgtatgaa cataccatta attgttcctt ctctagttga taattatcga aaaataaata
      601 tagcaaataa taaatcaaac aacgatttaa ccaaaagaga aaaagaatgt ttagcgtggg
      661 catgcgaagg aaaaagctct tgggatattt caaaaatatt aggttgcagt gagcgtactg
      721 tcactttcca tttaaccaat gcgcaaatga aactcaatac aacaaaccgc tgccaaagta
      781 tttctaaagc aattttaaca ggagcaattg attgcccata ctttaaaaat taataacact
      841 gatagtgcta gtgtagatca ctactagagc caggcatcaa ataaaacgaa aggctcagtc
      901 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca
      961 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagacct gtaggatcgt
     1021 acaggtttac gcaagaaaat ggtttgttat agtcgaataa atactagagt cacacaggaa
     1081 agtactagat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg
     1141 agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg
     1201 ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct
     1261 ggcccaccct cgtgaccacc ttcggctacg gcctgcaatg cttcgcccgc taccccgacc
     1321 acatgaagct gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca
     1381 ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg
     1441 acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc
     1501 tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc
     1561 agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc
     1621 agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg
     1681 acaaccacta cctgagctac cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc
     1741 acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt
     1801 acaagtaata atactagaga aagaggagaa atactagatg actataatga taaaaaaatc
     1861 ggattttttg gcaattccat cggaggagta taaaggtatt ctaagtcttc gttatcaagt
     1921 gtttaagcaa agacttgagt gggacttagt tgtagaaaat aaccttgaat cagatgagta
     1981 tgataactca aatgcagaat atatttatgc ttgtgatgat actgaaaatg taagtggatg
     2041 ctggcgttta ttacctacaa caggtgatta tatgctgaaa agtgtttttc ctgaattgct
     2101 tggtcaacag agtgctccca aagatcctaa tatagtcgaa ttaagtcgtt ttgctgtagg
     2161 taaaaatagc tcaaagataa ataactctgc tagtgaaatt acaatgaaac tatttgaagc
     2221 tatatataaa cacgctgtta gtcaaggtat tacagaatat gtaacagtaa catcaacagc
     2281 aatagagcga tttttaaagc gtattaaagt tccttgtcat cgtattggag acaaagaaat
     2341 tcatgtatta ggtgatacta aatcggttgt attgtctatg cctattaatg aacagtttaa
     2401 aaaagcagtc ttaaatgctg caaacgacga aaactacgct ttagtagctt aataactctg
     2461 atagtgctag tgtagatctc tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg
     2521 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac
     2581 actggctcac cttcgggtgg gcctttctgc gtttata
//