##gff-version 3
##sequence-region BBa_J46001 1 2617
BBa_J46001	.	engineered_region	1070	1811	.	+	0	ID=BBa_I15017;Name=BBa_I15017
BBa_J46001	.	ribosome_entry_site	1070	1082	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_I15017
BBa_J46001	.	engineered_region	1070	1082	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_J46001	.	ribosome_entry_site	1070	1082	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_J46001	.	ribosome_entry_site	1076	1079	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_J46001	.	CDS	1089	1811	.	+	0	ID=BBa_E0030;Name=BBa_E0030;Parent=BBa_I15017
BBa_J46001	.	engineered_region	1820	2617	.	+	0	ID=BBa_F1610;Name=BBa_F1610
BBa_J46001	.	terminator	2577	2617	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_F1610
BBa_J46001	.	polyA_site	2604	2617	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J46001	.	engineered_region	2577	2617	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J46001	.	stem_loop	2584	2603	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J46001	.	stop_codon	2610	2610	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J46001	.	CDS	1838	2455	.	+	0	ID=BBa_C0061;Name=BBa_C0061;Parent=BBa_F1610
BBa_J46001	.	CDS	1838	2416	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_J46001	.	engineered_region	1838	2455	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_J46001	.	tag	2417	2448	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_J46001	.	engineered_tag	2456	2480	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_J46001	.	terminator	2489	2568	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_F1610
BBa_J46001	.	stem_loop	2500	2543	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J46001	.	engineered_region	2489	2568	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J46001	.	ribosome_entry_site	1820	1831	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_F1610
BBa_J46001	.	conserved_region	1824	1827	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J46001	.	engineered_region	1	1061	.	+	0	ID=BBa_F2620;Name=BBa_F2620
BBa_J46001	.	CDS	81	836	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_F2620
BBa_J46001	.	CDS	81	830	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_J46001	.	engineered_tag	837	861	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_J46001	.	engineered_region	81	836	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_J46001	.	point_mutation	254	254	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_J46001	.	engineered_region	81	82	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_J46001	.	point_mutation	572	572	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_J46001	.	promoter	1007	1061	.	+	0	ID=BBa_R0062;Name=BBa_R0062;Parent=BBa_F2620
BBa_J46001	.	engineered_region	1007	1061	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_J46001	.	promoter	1026	1031	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_J46001	.	promoter	1048	1053	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_J46001	.	start_codon	1059	1059	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_J46001	.	non_covalent_binding_site	1007	1026	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_J46001	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_F2620
BBa_J46001	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_J46001	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_J46001	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_J46001	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_J46001	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_J46001	.	ribosome_entry_site	63	74	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_F2620
BBa_J46001	.	conserved_region	67	70	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J46001	.	terminator	870	998	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_F2620
BBa_J46001	.	terminator	958	998	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_J46001	.	polyA_site	985	998	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J46001	.	engineered_region	958	998	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J46001	.	stem_loop	965	984	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J46001	.	stop_codon	991	991	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J46001	.	terminator	870	949	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_J46001	.	stem_loop	881	924	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J46001	.	engineered_region	870	949	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
>BBa_J46001
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatacta
gatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaat
caatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggtta
aatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatga
tcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaa
tctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatg
gcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacat
accattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaacc
aaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtg
agcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagc
aattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagag
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctct
ctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtt
tacgcaagaaaatggtttgttatagtcgaataaatactagagtcacacaggaaagtactagatggtgagcaagggcgag
gagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcg
agggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcc
caccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttc
aagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccg
aggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcct
ggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtg
aacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcg
acggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcga
tcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaataatactag
agaaagaggagaaatactagatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaagg
tattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagat
gagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattac
ctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatat
agtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaacta
tttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgat
ttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgt
attgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagct
taataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactg
ggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttc
tgcgtttata