##gff-version 3
##sequence-region BBa_J46002 1 5391
BBa_J46002	.	engineered_region	1	2766	.	+	0	ID=BBa_J46000;Name=BBa_J46000
BBa_J46002	.	engineered_region	1219	1960	.	+	0	ID=BBa_I15016;Name=BBa_I15016;Parent=BBa_J46000
BBa_J46002	.	CDS	1238	1960	.	+	0	ID=BBa_E0020;Name=BBa_E0020;Parent=BBa_I15016
BBa_J46002	.	ribosome_entry_site	1219	1231	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_I15016
BBa_J46002	.	ribosome_entry_site	1225	1228	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_J46002	.	engineered_region	1219	1231	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_J46002	.	ribosome_entry_site	1219	1231	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_J46002	.	engineered_region	1969	2766	.	+	0	ID=BBa_F1610;Name=BBa_F1610;Parent=BBa_J46000
BBa_J46002	.	terminator	2726	2766	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_F1610
BBa_J46002	.	engineered_region	2726	2766	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J46002	.	stop_codon	2759	2759	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J46002	.	stem_loop	2733	2752	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J46002	.	polyA_site	2753	2766	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J46002	.	CDS	1987	2604	.	+	0	ID=BBa_C0061;Name=BBa_C0061;Parent=BBa_F1610
BBa_J46002	.	engineered_region	1987	2604	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_J46002	.	tag	2566	2597	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_J46002	.	engineered_tag	2605	2629	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_J46002	.	CDS	1987	2565	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_J46002	.	ribosome_entry_site	1969	1980	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_F1610
BBa_J46002	.	conserved_region	1973	1976	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J46002	.	terminator	2638	2717	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_F1610
BBa_J46002	.	stem_loop	2649	2692	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J46002	.	engineered_region	2638	2717	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J46002	.	promoter	1	1210	.	+	0	ID=BBa_I0500;Name=BBa_I0500;Parent=BBa_J46000
BBa_J46002	.	promoter	1043	1114	.	+	0	ID=annotation1498842;Name=PC;Parent=BBa_I0500
BBa_J46002	.	promoter	1080	1210	.	+	0	ID=annotation1498841;Name=Pbad;Parent=BBa_I0500
BBa_J46002	.	sequence_feature	1	1079	.	+	0	ID=annotation1498843;Name=AraC;Parent=BBa_I0500
BBa_J46002	.	engineered_region	2775	5391	.	+	0	ID=BBa_J46001;Name=BBa_J46001
BBa_J46002	.	engineered_region	3844	4585	.	+	0	ID=BBa_I15017;Name=BBa_I15017;Parent=BBa_J46001
BBa_J46002	.	CDS	3863	4585	.	+	0	ID=BBa_E0030;Name=BBa_E0030;Parent=BBa_I15017
BBa_J46002	.	ribosome_entry_site	3844	3856	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_I15017
BBa_J46002	.	ribosome_entry_site	3850	3853	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_J46002	.	engineered_region	3844	3856	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_J46002	.	ribosome_entry_site	3844	3856	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_J46002	.	engineered_region	4594	5391	.	+	0	ID=BBa_F1610;Name=BBa_F1610;Parent=BBa_J46001
BBa_J46002	.	terminator	5351	5391	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_F1610
BBa_J46002	.	engineered_region	5351	5391	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J46002	.	stop_codon	5384	5384	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J46002	.	stem_loop	5358	5377	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J46002	.	polyA_site	5378	5391	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J46002	.	CDS	4612	5229	.	+	0	ID=BBa_C0061;Name=BBa_C0061;Parent=BBa_F1610
BBa_J46002	.	engineered_region	4612	5229	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_J46002	.	tag	5191	5222	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_J46002	.	engineered_tag	5230	5254	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_J46002	.	CDS	4612	5190	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_J46002	.	ribosome_entry_site	4594	4605	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_F1610
BBa_J46002	.	conserved_region	4598	4601	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J46002	.	terminator	5263	5342	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_F1610
BBa_J46002	.	stem_loop	5274	5317	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J46002	.	engineered_region	5263	5342	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J46002	.	engineered_region	2775	3835	.	+	0	ID=BBa_F2620;Name=BBa_F2620;Parent=BBa_J46001
BBa_J46002	.	ribosome_entry_site	2837	2848	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_F2620
BBa_J46002	.	conserved_region	2841	2844	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J46002	.	terminator	3644	3772	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_F2620
BBa_J46002	.	terminator	3644	3723	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_J46002	.	stem_loop	3655	3698	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J46002	.	engineered_region	3644	3723	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J46002	.	terminator	3732	3772	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_J46002	.	engineered_region	3732	3772	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J46002	.	stop_codon	3765	3765	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J46002	.	stem_loop	3739	3758	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J46002	.	polyA_site	3759	3772	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J46002	.	promoter	2775	2828	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_F2620
BBa_J46002	.	promoter	2794	2799	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_J46002	.	non_covalent_binding_site	2800	2818	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_J46002	.	engineered_region	2775	2828	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_J46002	.	non_covalent_binding_site	2775	2793	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_J46002	.	promoter	2817	2822	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_J46002	.	CDS	2855	3610	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_F2620
BBa_J46002	.	CDS	2855	3604	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_J46002	.	engineered_region	2855	3610	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_J46002	.	engineered_tag	3611	3635	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_J46002	.	point_mutation	3028	3028	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_J46002	.	engineered_region	2855	2856	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_J46002	.	point_mutation	3346	3346	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_J46002	.	promoter	3781	3835	.	+	0	ID=BBa_R0062;Name=BBa_R0062;Parent=BBa_F2620
BBa_J46002	.	non_covalent_binding_site	3781	3800	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_J46002	.	promoter	3822	3827	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_J46002	.	engineered_region	3781	3835	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_J46002	.	start_codon	3833	3833	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_J46002	.	promoter	3800	3805	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
>BBa_J46002
ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcat
tttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtgg
tgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaa
aagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtga
tcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgcc
gcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttg
cccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccag
ttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgac
cgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcac
caccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataa
ccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcg
cttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactg
cgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaa
gccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacac
tttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttc
tccatacccgtttttttgggctagctactagagtcacacaggaaagtactagatggtgagcaagggcgaggagctgttc
accggggtggtgcccatcctggtcgagctggacggcgacgtgaacggccacaagttcagcgtgtccggcgagggcgagg
gcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgt
gaccaccctgacctggggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgcc
atgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagt
tcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaa
gctggagtacaactacatcagccacaacgtctatatcaccgccgacaagcagaagaacggcatcaaggccaacttcaag
atccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccg
tgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggt
cctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaataatactagagaaagagg
agaaatactagatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaag
tcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgat
aactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacag
gtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaatt
aagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagct
atatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagc
gtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctat
gcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactc
tgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcg
ttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttat
atactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagagga
gaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataat
gatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcatt
ctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaat
aaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgta
aataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacgg
ctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtg
tatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaac
gatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattag
gttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtat
ttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatca
ctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggt
gaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatc
gtacaggtttacgcaagaaaatggtttgttatagtcgaataaatactagagtcacacaggaaagtactagatggtgagc
aagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcg
tgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgt
gccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcac
gacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaaga
cccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacgg
caacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggc
atcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacaccc
ccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacga
gaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaa
taatactagagaaagaggagaaatactagatgactataatgataaaaaaatcggattttttggcaattccatcggagga
gtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataacctt
gaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggc
gtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaaga
tcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattaca
atgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaa
tagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaa
atcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgct
ttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtc
gaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggt
gggcctttctgcgtttata