LOCUS       BBa_J46003              2881 bp    DNA     linear       25-Nov-2024
DEFINITION  Aspartate detector
ACCESSION   BBa_J46003
VERSION     BBa_J46003.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    1..220
                     /label=BBa_J04500
     misc_feature    649..650
                     /label=A
     misc_feature    1679..1711
                     /label=LVA
     CDS             227..991
                     /label=Tar (front)
     CDS             998..1678
                     /label=Envz (back)
     misc_feature    1718..1742
                     /label=Help:Barcodes
     CDS             227..1742
                     /label=BBa_C0082
     stem_loop       1762..1805
                     /label=stem_loop
     misc_feature    1751..1830
                     /label=BBa_B0010
     terminator      1751..1830
                     /label=BBa_B0010
     stem_loop       1846..1865
                     /label=T7 TE
     misc_feature    1872..1872
                     /label=stop
     polyA_site      1866..1879
                     /label=polya
     misc_feature    1839..1879
                     /label=BBa_B0012
     terminator      1839..1879
                     /label=BBa_B0012
     terminator      1751..1879
                     /label=BBa_B0015
     misc_feature    1900..1917
                     /label=C1 OmpR
     misc_feature    1921..1938
                     /label=C2 OmpR
     misc_feature    1941..1958
                     /label=C3 OmpR
     promoter        1962..1967
                     /label=-35
     promoter        1985..1990
                     /label=-10
     promoter        1888..1995
                     /label=BBa_R0082
     misc_feature    2008..2011
                     /label=conserved
     RBS             2004..2015
                     /label=BBa_B0034
     CDS             2022..2744
                     /label=BBa_E0030
     stem_loop       2764..2807
                     /label=stem_loop
     misc_feature    2753..2832
                     /label=BBa_B0010
     terminator      2753..2832
                     /label=BBa_B0010
     stem_loop       2848..2867
                     /label=T7 TE
     misc_feature    2874..2874
                     /label=stop
     polyA_site      2868..2881
                     /label=polya
     misc_feature    2841..2881
                     /label=BBa_B0012
     terminator      2841..2881
                     /label=BBa_B0012
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatga ttaaccgtat
      241 ccgcgtagtc acgctgttgg taatggtgct gggggtattc gcactgttac agcttatttc
      301 cggcagtctg tttttttctt cccttcacca tagccagaag agctttgtgg tttccaatca
      361 attacgggaa cagcagggcg agctgacgtc aacctgggat ttaatgctgc aaacgcgcat
      421 taacctgagt cgttcagcgg tacggatgat gatggattcc tccaatcaac aaagtaacgc
      481 caaagttgaa ttgctcgata gcgccaggaa aacattggcg caggcagcga cgcattataa
      541 aaaattcaaa agcatggcac cgttacctga aatggtcgct accagtcgta atattgatga
      601 aaaatataaa aactattaca cagcgttaac tgaactgatt gattatctcg attatggcaa
      661 tactggagct tatttcgctc agccaaccca gggaatgcaa aatgcaatgg gcgaagcgtt
      721 tgctcagtac gccctcagca gtgaaaaact gtatcgcgat atcgtcactg acaacgcaga
      781 tgattaccga tttgcccagt ggcaactggc ggttatcgcg ctggtggtgg tattgattct
      841 gctggtggcg tggtacggca ttcgccgtat gttgcttact ccgctggcaa aaattattgc
      901 tcacattcgc gaaatcgccg gtggtaacct ggcgaatacc ctgaccattg acgggcgcag
      961 tgaaatgggc gacctggcgc agagcgtttc acatatggcg gctggtgtta agcaactggc
     1021 ggatgaccgc acgctgctga tggcgggggt aagtcacgac ttgcgcacgc cgctgacgcg
     1081 tattcgcctg gcgactgaga tgatgagcga gcaggatggc tatctggcag aatcgatcaa
     1141 taaagatatc gaagagtgca acgccatcat tgagcagttt atcgactacc tgcgcaccgg
     1201 gcaggagatg ccgatggaaa tggcggatct taatgcagta ctcggtgagg tgattgctgc
     1261 cgaaagtggc tatgagcggg aaattgaaac cgcgctttac cccggcagca ttgaagtgaa
     1321 aatgcacccg ctgtcgatca aacgcgcggt ggcgaatatg gtggtcaacg ccgcccgtta
     1381 tggcaatggc tggatcaaag tcagcagcgg aacggagccg aatcgcgcct ggttccaggt
     1441 ggaagatgac ggtccgggaa ttgcgccgga acaacgtaag cacctgttcc agccgtttgt
     1501 ccgcggcgac agtgcgcgca ccattagcgg cacgggatta gggctggcaa ttgtgcagcg
     1561 tatcgtggat aaccataacg ggatgctgga gcttggcacc agcgagcggg gcgggctttc
     1621 cattcgcgcc tggctgccag tgccggtaac gcgggcgcag ggcacgacaa aagaaggggc
     1681 tgcaaacgac gaaaactacg ctttagtagc ttaataacgc tgatagtgct agtgtagatc
     1741 gctactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt
     1801 tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt
     1861 gggcctttct gcgtttatat actagagtcc cttgcattta cattttgaaa catctatagc
     1921 gataaatgaa acatcttaaa agttttagta tcatattcgt gttggattat tctgcatttt
     1981 tggggagaat ggacttacta gagaaagagg agaaatacta gatggtgagc aagggcgagg
     2041 agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca
     2101 agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt
     2161 tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accttcggct
     2221 acggcctgca atgcttcgcc cgctaccccg accacatgaa gctgcacgac ttcttcaagt
     2281 ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact
     2341 acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga
     2401 agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca
     2461 acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca
     2521 agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca
     2581 cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc taccagtccg
     2641 ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg
     2701 ccgccgggat cactctcggc atggacgagc tgtacaagta ataatactag agccaggcat
     2761 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg
     2821 gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat
     2881 a
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