##gff-version 3
##sequence-region BBa_J46003 1 2881
BBa_J46003	.	CDS	2022	2744	.	+	0	ID=BBa_E0030;Name=BBa_E0030
BBa_J46003	.	promoter	1888	1995	.	+	0	ID=BBa_R0082;Name=BBa_R0082
BBa_J46003	.	promoter	1962	1967	.	+	0	ID=annotation301166;Name=-35;Parent=BBa_R0082
BBa_J46003	.	non_covalent_binding_site	1941	1958	.	+	0	ID=annotation301156;Name=C3 OmpR;Parent=BBa_R0082
BBa_J46003	.	non_covalent_binding_site	1900	1917	.	+	0	ID=annotation301154;Name=C1 OmpR;Parent=BBa_R0082
BBa_J46003	.	promoter	1985	1990	.	+	0	ID=annotation301167;Name=-10;Parent=BBa_R0082
BBa_J46003	.	non_covalent_binding_site	1921	1938	.	+	0	ID=annotation301155;Name=C2 OmpR;Parent=BBa_R0082
BBa_J46003	.	terminator	2753	2832	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_J46003	.	engineered_region	2753	2832	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J46003	.	stem_loop	2764	2807	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J46003	.	terminator	1751	1879	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_J46003	.	terminator	1839	1879	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_J46003	.	engineered_region	1839	1879	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J46003	.	stem_loop	1846	1865	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J46003	.	stop_codon	1872	1872	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J46003	.	polyA_site	1866	1879	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J46003	.	terminator	1751	1830	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_J46003	.	engineered_region	1751	1830	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J46003	.	stem_loop	1762	1805	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J46003	.	ribosome_entry_site	2004	2015	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_J46003	.	conserved_region	2008	2011	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J46003	.	terminator	2841	2881	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_J46003	.	engineered_region	2841	2881	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J46003	.	stem_loop	2848	2867	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J46003	.	stop_codon	2874	2874	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J46003	.	polyA_site	2868	2881	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J46003	.	CDS	227	1742	.	+	0	ID=BBa_C0082;Name=BBa_C0082
BBa_J46003	.	tag	1679	1711	.	+	0	ID=annotation301146;Name=LVA;Parent=BBa_C0082
BBa_J46003	.	CDS	998	1678	.	+	0	ID=annotation308360;Name=Envz (back);Parent=BBa_C0082
BBa_J46003	.	CDS	227	991	.	+	0	ID=annotation308352;Name=Tar (front);Parent=BBa_C0082
BBa_J46003	.	engineered_tag	1718	1742	.	+	0	ID=annotation2213997;Name=Help:Barcodes;Parent=BBa_C0082
BBa_J46003	.	point_mutation	649	650	.	+	0	ID=annotation300943;Name=A;Parent=BBa_C0082
BBa_J46003	.	engineered_region	1	220	.	+	0	ID=BBa_J04500;Name=BBa_J04500
BBa_J46003	.	promoter	1	200	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_J04500
BBa_J46003	.	non_covalent_binding_site	89	126	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_J46003	.	engineered_region	1	200	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_J46003	.	promoter	161	166	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_J46003	.	start_codon	173	173	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_J46003	.	non_covalent_binding_site	166	200	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_J46003	.	CDS	1	88	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_J46003	.	promoter	137	142	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_J46003	.	ribosome_entry_site	209	220	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J04500
BBa_J46003	.	conserved_region	213	216	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_J46003
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc
gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct
cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgattaaccg
tatccgcgtagtcacgctgttggtaatggtgctgggggtattcgcactgttacagcttatttccggcagtctgtttttt
tcttcccttcaccatagccagaagagctttgtggtttccaatcaattacgggaacagcagggcgagctgacgtcaacct
gggatttaatgctgcaaacgcgcattaacctgagtcgttcagcggtacggatgatgatggattcctccaatcaacaaag
taacgccaaagttgaattgctcgatagcgccaggaaaacattggcgcaggcagcgacgcattataaaaaattcaaaagc
atggcaccgttacctgaaatggtcgctaccagtcgtaatattgatgaaaaatataaaaactattacacagcgttaactg
aactgattgattatctcgattatggcaatactggagcttatttcgctcagccaacccagggaatgcaaaatgcaatggg
cgaagcgtttgctcagtacgccctcagcagtgaaaaactgtatcgcgatatcgtcactgacaacgcagatgattaccga
tttgcccagtggcaactggcggttatcgcgctggtggtggtattgattctgctggtggcgtggtacggcattcgccgta
tgttgcttactccgctggcaaaaattattgctcacattcgcgaaatcgccggtggtaacctggcgaataccctgaccat
tgacgggcgcagtgaaatgggcgacctggcgcagagcgtttcacatatggcggctggtgttaagcaactggcggatgac
cgcacgctgctgatggcgggggtaagtcacgacttgcgcacgccgctgacgcgtattcgcctggcgactgagatgatga
gcgagcaggatggctatctggcagaatcgatcaataaagatatcgaagagtgcaacgccatcattgagcagtttatcga
ctacctgcgcaccgggcaggagatgccgatggaaatggcggatcttaatgcagtactcggtgaggtgattgctgccgaa
agtggctatgagcgggaaattgaaaccgcgctttaccccggcagcattgaagtgaaaatgcacccgctgtcgatcaaac
gcgcggtggcgaatatggtggtcaacgccgcccgttatggcaatggctggatcaaagtcagcagcggaacggagccgaa
tcgcgcctggttccaggtggaagatgacggtccgggaattgcgccggaacaacgtaagcacctgttccagccgtttgtc
cgcggcgacagtgcgcgcaccattagcggcacgggattagggctggcaattgtgcagcgtatcgtggataaccataacg
ggatgctggagcttggcaccagcgagcggggcgggctttccattcgcgcctggctgccagtgccggtaacgcgggcgca
gggcacgacaaaagaaggggctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtaga
tcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtc
ggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtcccttgca
tttacattttgaaacatctatagcgataaatgaaacatcttaaaagttttagtatcatattcgtgttggattattctgc
atttttggggagaatggacttactagagaaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggg
gtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatg
ccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccac
cttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgccc
gaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagg
gcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctgga
gtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgc
cacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgc
tgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgct
ggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaataatactagagccaggcatcaaat
aaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcac
actggctcaccttcgggtgggcctttctgcgtttata