LOCUS       BBa_J47050              1353 bp    DNA     linear       22-Dec-2024
DEFINITION  Constitutive device for lacI repressor, strong RBS
ACCESSION   BBa_J47050
VERSION     BBa_J47050.1  
FEATURES             Location/Qualifiers
     misc_feature    1..37
                     /label=P(Lac) IQ
     promoter        3..8
                     /label=-35
     promoter        26..31
                     /label=-10
     promoter        1..37
                     /label=BBa_I14032
     misc_feature    50..53
                     /label=conserved
     RBS             46..57
                     /label=BBa_B0034
     misc_feature    1153..1191
                     /label=LVA
     CDS             64..1191
                     /label=lacI-LVA
     misc_feature    64..1191
                     /label=BBa_C0012
     misc_feature    1192..1216
                     /label=Help:Barcodes
     CDS             64..1191
                     /label=BBa_C0012
     stem_loop       1236..1279
                     /label=stem_loop
     misc_feature    1225..1304
                     /label=BBa_B0010
     terminator      1225..1304
                     /label=BBa_B0010
     stem_loop       1320..1339
                     /label=T7 TE
     misc_feature    1346..1346
                     /label=stop
     polyA_site      1340..1353
                     /label=polya
     misc_feature    1313..1353
                     /label=BBa_B0012
     terminator      1313..1353
                     /label=BBa_B0012
     terminator      1225..1353
                     /label=BBa_B0015
ORIGIN
        1 tggtgcaaaa cctttcgcgg tatggcatga tagcgcctac tagagaaaga ggagaaatac
       61 tagatggtga atgtgaaacc agtaacgtta tacgatgtcg cagagtatgc cggtgtctct
      121 tatcagaccg tttcccgcgt ggtgaaccag gccagccacg tttctgcgaa aacgcgggaa
      181 aaagtggaag cggcgatggc ggagctgaat tacattccca accgcgtggc acaacaactg
      241 gcgggcaaac agtcgttgct gattggcgtt gccacctcca gtctggccct gcacgcgccg
      301 tcgcaaattg tcgcggcgat taaatctcgc gccgatcaac tgggtgccag cgtggtggtg
      361 tcgatggtag aacgaagcgg cgtcgaagcc tgtaaagcgg cggtgcacaa tcttctcgcg
      421 caacgcgtca gtgggctgat cattaactat ccgctggatg accaggatgc cattgctgtg
      481 gaagctgcct gcactaatgt tccggcgtta tttcttgatg tctctgacca gacacccatc
      541 aacagtatta ttttctccca tgaagacggt acgcgactgg gcgtggagca tctggtcgca
      601 ttgggtcacc agcaaatcgc gctgttagcg ggcccattaa gttctgtctc ggcgcgtctg
      661 cgtctggctg gctggcataa atatctcact cgcaatcaaa ttcagccgat agcggaacgg
      721 gaaggcgact ggagtgccat gtccggtttt caacaaacca tgcaaatgct gaatgagggc
      781 atcgttccca ctgcgatgct ggttgccaac gatcagatgg cgctgggcgc aatgcgcgcc
      841 attaccgagt ccgggctgcg cgttggtgcg gatatctcgg tagtgggata cgacgatacc
      901 gaagacagct catgttatat cccgccgtta accaccatca aacaggattt tcgcctgctg
      961 gggcaaacca gcgtggaccg cttgctgcaa ctctctcagg gccaggcggt gaagggcaat
     1021 cagctgttgc ccgtctcact ggtgaaaaga aaaaccaccc tggcgcccaa tacgcaaacc
     1081 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg
     1141 gaaagcgggc aggctgcaaa cgacgaaaac tacgctttag tagcttaata actctgatag
     1201 tgctagtgta gatctctact agagccaggc atcaaataaa acgaaaggct cagtcgaaag
     1261 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg
     1321 gctcaccttc gggtgggcct ttctgcgttt ata
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