LOCUS       BBa_J47051              1356 bp    DNA     linear       03-May-2024
DEFINITION  Constitutive device for lacI repressor, strong RBS
ACCESSION   BBa_J47051
VERSION     BBa_J47051.1  
FEATURES             Location/Qualifiers
     misc_feature    1..37
                     /label=P(Lac) IQ
     promoter        3..8
                     /label=-35
     promoter        26..31
                     /label=-10
     promoter        1..37
                     /label=BBa_I14032
     RBS             46..60
                     /label=RBS-1\Strong
     RBS             53..57
                     /label=RBS
     misc_feature    46..60
                     /label=BBa_B0030
     RBS             46..60
                     /label=BBa_B0030
     misc_feature    1156..1194
                     /label=LVA
     CDS             67..1194
                     /label=lacI-LVA
     misc_feature    67..1194
                     /label=BBa_C0012
     misc_feature    1195..1219
                     /label=Help:Barcodes
     CDS             67..1194
                     /label=BBa_C0012
     stem_loop       1239..1282
                     /label=stem_loop
     misc_feature    1228..1307
                     /label=BBa_B0010
     terminator      1228..1307
                     /label=BBa_B0010
     stem_loop       1323..1342
                     /label=T7 TE
     misc_feature    1349..1349
                     /label=stop
     polyA_site      1343..1356
                     /label=polya
     misc_feature    1316..1356
                     /label=BBa_B0012
     terminator      1316..1356
                     /label=BBa_B0012
     terminator      1228..1356
                     /label=BBa_B0015
ORIGIN
        1 tggtgcaaaa cctttcgcgg tatggcatga tagcgcctac tagagattaa agaggagaaa
       61 tactagatgg tgaatgtgaa accagtaacg ttatacgatg tcgcagagta tgccggtgtc
      121 tcttatcaga ccgtttcccg cgtggtgaac caggccagcc acgtttctgc gaaaacgcgg
      181 gaaaaagtgg aagcggcgat ggcggagctg aattacattc ccaaccgcgt ggcacaacaa
      241 ctggcgggca aacagtcgtt gctgattggc gttgccacct ccagtctggc cctgcacgcg
      301 ccgtcgcaaa ttgtcgcggc gattaaatct cgcgccgatc aactgggtgc cagcgtggtg
      361 gtgtcgatgg tagaacgaag cggcgtcgaa gcctgtaaag cggcggtgca caatcttctc
      421 gcgcaacgcg tcagtgggct gatcattaac tatccgctgg atgaccagga tgccattgct
      481 gtggaagctg cctgcactaa tgttccggcg ttatttcttg atgtctctga ccagacaccc
      541 atcaacagta ttattttctc ccatgaagac ggtacgcgac tgggcgtgga gcatctggtc
      601 gcattgggtc accagcaaat cgcgctgtta gcgggcccat taagttctgt ctcggcgcgt
      661 ctgcgtctgg ctggctggca taaatatctc actcgcaatc aaattcagcc gatagcggaa
      721 cgggaaggcg actggagtgc catgtccggt tttcaacaaa ccatgcaaat gctgaatgag
      781 ggcatcgttc ccactgcgat gctggttgcc aacgatcaga tggcgctggg cgcaatgcgc
      841 gccattaccg agtccgggct gcgcgttggt gcggatatct cggtagtggg atacgacgat
      901 accgaagaca gctcatgtta tatcccgccg ttaaccacca tcaaacagga ttttcgcctg
      961 ctggggcaaa ccagcgtgga ccgcttgctg caactctctc agggccaggc ggtgaagggc
     1021 aatcagctgt tgcccgtctc actggtgaaa agaaaaacca ccctggcgcc caatacgcaa
     1081 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga
     1141 ctggaaagcg ggcaggctgc aaacgacgaa aactacgctt tagtagctta ataactctga
     1201 tagtgctagt gtagatctct actagagcca ggcatcaaat aaaacgaaag gctcagtcga
     1261 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca
     1321 ctggctcacc ttcgggtggg cctttctgcg tttata
//