LOCUS       BBa_J47054              1357 bp    DNA     linear       22-Dec-2024
DEFINITION  Constitutive device (medium transcription)  for lacI repressor, 
            strong RBS
ACCESSION   BBa_J47054
VERSION     BBa_J47054.1  
FEATURES             Location/Qualifiers
     promoter        4..9
                     /label=-35
     promoter        27..32
                     /label=-10
     misc_feature    1..38
                     /label=P(Cat)
     promoter        1..38
                     /label=BBa_I14033
     RBS             47..61
                     /label=RBS-1\Strong
     RBS             54..58
                     /label=RBS
     misc_feature    47..61
                     /label=BBa_B0030
     RBS             47..61
                     /label=BBa_B0030
     misc_feature    1157..1195
                     /label=LVA
     CDS             68..1195
                     /label=lacI-LVA
     misc_feature    68..1195
                     /label=BBa_C0012
     misc_feature    1196..1220
                     /label=Help:Barcodes
     CDS             68..1195
                     /label=BBa_C0012
     stem_loop       1240..1283
                     /label=stem_loop
     misc_feature    1229..1308
                     /label=BBa_B0010
     terminator      1229..1308
                     /label=BBa_B0010
     stem_loop       1324..1343
                     /label=T7 TE
     misc_feature    1350..1350
                     /label=stop
     polyA_site      1344..1357
                     /label=polya
     misc_feature    1317..1357
                     /label=BBa_B0012
     terminator      1317..1357
                     /label=BBa_B0012
     terminator      1229..1357
                     /label=BBa_B0015
ORIGIN
        1 ggcacgtaag aggttccaac tttcaccata atgaaacata ctagagatta aagaggagaa
       61 atactagatg gtgaatgtga aaccagtaac gttatacgat gtcgcagagt atgccggtgt
      121 ctcttatcag accgtttccc gcgtggtgaa ccaggccagc cacgtttctg cgaaaacgcg
      181 ggaaaaagtg gaagcggcga tggcggagct gaattacatt cccaaccgcg tggcacaaca
      241 actggcgggc aaacagtcgt tgctgattgg cgttgccacc tccagtctgg ccctgcacgc
      301 gccgtcgcaa attgtcgcgg cgattaaatc tcgcgccgat caactgggtg ccagcgtggt
      361 ggtgtcgatg gtagaacgaa gcggcgtcga agcctgtaaa gcggcggtgc acaatcttct
      421 cgcgcaacgc gtcagtgggc tgatcattaa ctatccgctg gatgaccagg atgccattgc
      481 tgtggaagct gcctgcacta atgttccggc gttatttctt gatgtctctg accagacacc
      541 catcaacagt attattttct cccatgaaga cggtacgcga ctgggcgtgg agcatctggt
      601 cgcattgggt caccagcaaa tcgcgctgtt agcgggccca ttaagttctg tctcggcgcg
      661 tctgcgtctg gctggctggc ataaatatct cactcgcaat caaattcagc cgatagcgga
      721 acgggaaggc gactggagtg ccatgtccgg ttttcaacaa accatgcaaa tgctgaatga
      781 gggcatcgtt cccactgcga tgctggttgc caacgatcag atggcgctgg gcgcaatgcg
      841 cgccattacc gagtccgggc tgcgcgttgg tgcggatatc tcggtagtgg gatacgacga
      901 taccgaagac agctcatgtt atatcccgcc gttaaccacc atcaaacagg attttcgcct
      961 gctggggcaa accagcgtgg accgcttgct gcaactctct cagggccagg cggtgaaggg
     1021 caatcagctg ttgcccgtct cactggtgaa aagaaaaacc accctggcgc ccaatacgca
     1081 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
     1141 actggaaagc gggcaggctg caaacgacga aaactacgct ttagtagctt aataactctg
     1201 atagtgctag tgtagatctc tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg
     1261 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac
     1321 actggctcac cttcgggtgg gcctttctgc gtttata
//