LOCUS       BBa_J47055              1361 bp    DNA     linear       17-May-2024
DEFINITION  Constitutive device (medium transcription)  for lacI repressor, 
            strong RBS
ACCESSION   BBa_J47055
VERSION     BBa_J47055.1  
FEATURES             Location/Qualifiers
     promoter        1..45
                     /label=P(Kat)
     promoter        1..45
                     /label=BBa_I14034
     misc_feature    58..61
                     /label=conserved
     RBS             54..65
                     /label=BBa_B0034
     misc_feature    1161..1199
                     /label=LVA
     CDS             72..1199
                     /label=lacI-LVA
     misc_feature    72..1199
                     /label=BBa_C0012
     misc_feature    1200..1224
                     /label=Help:Barcodes
     CDS             72..1199
                     /label=BBa_C0012
     stem_loop       1244..1287
                     /label=stem_loop
     misc_feature    1233..1312
                     /label=BBa_B0010
     terminator      1233..1312
                     /label=BBa_B0010
     stem_loop       1328..1347
                     /label=T7 TE
     misc_feature    1354..1354
                     /label=stop
     polyA_site      1348..1361
                     /label=polya
     misc_feature    1321..1361
                     /label=BBa_B0012
     terminator      1321..1361
                     /label=BBa_B0012
     terminator      1233..1361
                     /label=BBa_B0015
ORIGIN
        1 cattattgca attaataaac aactaacgga caattctacc taacatacta gagaaagagg
       61 agaaatacta gatggtgaat gtgaaaccag taacgttata cgatgtcgca gagtatgccg
      121 gtgtctctta tcagaccgtt tcccgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa
      181 cgcgggaaaa agtggaagcg gcgatggcgg agctgaatta cattcccaac cgcgtggcac
      241 aacaactggc gggcaaacag tcgttgctga ttggcgttgc cacctccagt ctggccctgc
      301 acgcgccgtc gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg
      361 tggtggtgtc gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc
      421 ttctcgcgca acgcgtcagt gggctgatca ttaactatcc gctggatgac caggatgcca
      481 ttgctgtgga agctgcctgc actaatgttc cggcgttatt tcttgatgtc tctgaccaga
      541 cacccatcaa cagtattatt ttctcccatg aagacggtac gcgactgggc gtggagcatc
      601 tggtcgcatt gggtcaccag caaatcgcgc tgttagcggg cccattaagt tctgtctcgg
      661 cgcgtctgcg tctggctggc tggcataaat atctcactcg caatcaaatt cagccgatag
      721 cggaacggga aggcgactgg agtgccatgt ccggttttca acaaaccatg caaatgctga
      781 atgagggcat cgttcccact gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa
      841 tgcgcgccat taccgagtcc gggctgcgcg ttggtgcgga tatctcggta gtgggatacg
      901 acgataccga agacagctca tgttatatcc cgccgttaac caccatcaaa caggattttc
      961 gcctgctggg gcaaaccagc gtggaccgct tgctgcaact ctctcagggc caggcggtga
     1021 agggcaatca gctgttgccc gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata
     1081 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     1141 cccgactgga aagcgggcag gctgcaaacg acgaaaacta cgctttagta gcttaataac
     1201 tctgatagtg ctagtgtaga tctctactag agccaggcat caaataaaac gaaaggctca
     1261 gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag
     1321 tcacactggc tcaccttcgg gtgggccttt ctgcgtttat a
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