LOCUS       BBa_J47057              1363 bp    DNA     linear       29-Nov-2024
DEFINITION  Constitutive device (medium transcription)  for lacI repressor, 
            medium RBS
ACCESSION   BBa_J47057
VERSION     BBa_J47057.1  
FEATURES             Location/Qualifiers
     promoter        1..45
                     /label=P(Kat)
     promoter        1..45
                     /label=BBa_I14034
     misc_feature    60..63
                     /label=conserved
     RBS             54..67
                     /label=BBa_B0031
     misc_feature    1163..1201
                     /label=LVA
     CDS             74..1201
                     /label=lacI-LVA
     misc_feature    74..1201
                     /label=BBa_C0012
     misc_feature    1202..1226
                     /label=Help:Barcodes
     CDS             74..1201
                     /label=BBa_C0012
     stem_loop       1246..1289
                     /label=stem_loop
     misc_feature    1235..1314
                     /label=BBa_B0010
     terminator      1235..1314
                     /label=BBa_B0010
     stem_loop       1330..1349
                     /label=T7 TE
     misc_feature    1356..1356
                     /label=stop
     polyA_site      1350..1363
                     /label=polya
     misc_feature    1323..1363
                     /label=BBa_B0012
     terminator      1323..1363
                     /label=BBa_B0012
     terminator      1235..1363
                     /label=BBa_B0015
ORIGIN
        1 cattattgca attaataaac aactaacgga caattctacc taacatacta gagtcacaca
       61 ggaaacctac tagatggtga atgtgaaacc agtaacgtta tacgatgtcg cagagtatgc
      121 cggtgtctct tatcagaccg tttcccgcgt ggtgaaccag gccagccacg tttctgcgaa
      181 aacgcgggaa aaagtggaag cggcgatggc ggagctgaat tacattccca accgcgtggc
      241 acaacaactg gcgggcaaac agtcgttgct gattggcgtt gccacctcca gtctggccct
      301 gcacgcgccg tcgcaaattg tcgcggcgat taaatctcgc gccgatcaac tgggtgccag
      361 cgtggtggtg tcgatggtag aacgaagcgg cgtcgaagcc tgtaaagcgg cggtgcacaa
      421 tcttctcgcg caacgcgtca gtgggctgat cattaactat ccgctggatg accaggatgc
      481 cattgctgtg gaagctgcct gcactaatgt tccggcgtta tttcttgatg tctctgacca
      541 gacacccatc aacagtatta ttttctccca tgaagacggt acgcgactgg gcgtggagca
      601 tctggtcgca ttgggtcacc agcaaatcgc gctgttagcg ggcccattaa gttctgtctc
      661 ggcgcgtctg cgtctggctg gctggcataa atatctcact cgcaatcaaa ttcagccgat
      721 agcggaacgg gaaggcgact ggagtgccat gtccggtttt caacaaacca tgcaaatgct
      781 gaatgagggc atcgttccca ctgcgatgct ggttgccaac gatcagatgg cgctgggcgc
      841 aatgcgcgcc attaccgagt ccgggctgcg cgttggtgcg gatatctcgg tagtgggata
      901 cgacgatacc gaagacagct catgttatat cccgccgtta accaccatca aacaggattt
      961 tcgcctgctg gggcaaacca gcgtggaccg cttgctgcaa ctctctcagg gccaggcggt
     1021 gaagggcaat cagctgttgc ccgtctcact ggtgaaaaga aaaaccaccc tggcgcccaa
     1081 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
     1141 ttcccgactg gaaagcgggc aggctgcaaa cgacgaaaac tacgctttag tagcttaata
     1201 actctgatag tgctagtgta gatctctact agagccaggc atcaaataaa acgaaaggct
     1261 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag
     1321 agtcacactg gctcaccttc gggtgggcct ttctgcgttt ata
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