LOCUS       BBa_J47532              1373 bp    DNA     linear       26-Dec-2024
DEFINITION  luxR promoter + lacI device
ACCESSION   BBa_J47532
VERSION     BBa_J47532.1  
FEATURES             Location/Qualifiers
     misc_feature    1..20
                     /label=LuxR/HSL
     promoter        20..25
                     /label=-35
     promoter        42..47
                     /label=-10
     misc_feature    53..53
                     /label=start
     misc_feature    1..55
                     /label=BBa_R0062
     promoter        1..55
                     /label=BBa_R0062
     misc_feature    70..73
                     /label=conserved
     RBS             64..77
                     /label=BBa_B0031
     misc_feature    1173..1211
                     /label=LVA
     CDS             84..1211
                     /label=lacI-LVA
     misc_feature    84..1211
                     /label=BBa_C0012
     misc_feature    1212..1236
                     /label=Help:Barcodes
     CDS             84..1211
                     /label=BBa_C0012
     stem_loop       1256..1299
                     /label=stem_loop
     misc_feature    1245..1324
                     /label=BBa_B0010
     terminator      1245..1324
                     /label=BBa_B0010
     stem_loop       1340..1359
                     /label=T7 TE
     misc_feature    1366..1366
                     /label=stop
     polyA_site      1360..1373
                     /label=polya
     misc_feature    1333..1373
                     /label=BBa_B0012
     terminator      1333..1373
                     /label=BBa_B0012
     misc_feature    64..1373
                     /label=BBa_P0112
ORIGIN
        1 acctgtagga tcgtacaggt ttacgcaaga aaatggtttg ttatagtcga ataaatacta
       61 gagtcacaca ggaaacctac tagatggtga atgtgaaacc agtaacgtta tacgatgtcg
      121 cagagtatgc cggtgtctct tatcagaccg tttcccgcgt ggtgaaccag gccagccacg
      181 tttctgcgaa aacgcgggaa aaagtggaag cggcgatggc ggagctgaat tacattccca
      241 accgcgtggc acaacaactg gcgggcaaac agtcgttgct gattggcgtt gccacctcca
      301 gtctggccct gcacgcgccg tcgcaaattg tcgcggcgat taaatctcgc gccgatcaac
      361 tgggtgccag cgtggtggtg tcgatggtag aacgaagcgg cgtcgaagcc tgtaaagcgg
      421 cggtgcacaa tcttctcgcg caacgcgtca gtgggctgat cattaactat ccgctggatg
      481 accaggatgc cattgctgtg gaagctgcct gcactaatgt tccggcgtta tttcttgatg
      541 tctctgacca gacacccatc aacagtatta ttttctccca tgaagacggt acgcgactgg
      601 gcgtggagca tctggtcgca ttgggtcacc agcaaatcgc gctgttagcg ggcccattaa
      661 gttctgtctc ggcgcgtctg cgtctggctg gctggcataa atatctcact cgcaatcaaa
      721 ttcagccgat agcggaacgg gaaggcgact ggagtgccat gtccggtttt caacaaacca
      781 tgcaaatgct gaatgagggc atcgttccca ctgcgatgct ggttgccaac gatcagatgg
      841 cgctgggcgc aatgcgcgcc attaccgagt ccgggctgcg cgttggtgcg gatatctcgg
      901 tagtgggata cgacgatacc gaagacagct catgttatat cccgccgtta accaccatca
      961 aacaggattt tcgcctgctg gggcaaacca gcgtggaccg cttgctgcaa ctctctcagg
     1021 gccaggcggt gaagggcaat cagctgttgc ccgtctcact ggtgaaaaga aaaaccaccc
     1081 tggcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
     1141 cacgacaggt ttcccgactg gaaagcgggc aggctgcaaa cgacgaaaac tacgctttag
     1201 tagcttaata actctgatag tgctagtgta gatctctact agagccaggc atcaaataaa
     1261 acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc
     1321 tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt ata
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