##gff-version 3
##sequence-region BBa_J47532 1 1373
BBa_J47532	.	engineered_region	64	1373	.	+	0	ID=BBa_P0112;Name=BBa_P0112
BBa_J47532	.	terminator	1333	1373	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_P0112
BBa_J47532	.	polyA_site	1360	1373	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J47532	.	stop_codon	1366	1366	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J47532	.	stem_loop	1340	1359	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J47532	.	engineered_region	1333	1373	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J47532	.	CDS	84	1211	.	+	0	ID=BBa_C0012;Name=BBa_C0012;Parent=BBa_P0112
BBa_J47532	.	engineered_tag	1212	1236	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_J47532	.	CDS	84	1211	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_J47532	.	engineered_region	84	1211	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_J47532	.	tag	1173	1211	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_J47532	.	terminator	1245	1324	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_P0112
BBa_J47532	.	stem_loop	1256	1299	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J47532	.	engineered_region	1245	1324	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J47532	.	ribosome_entry_site	64	77	.	+	0	ID=BBa_B0031;Name=BBa_B0031;Parent=BBa_P0112
BBa_J47532	.	conserved_region	70	73	.	+	0	ID=annotation23316;Name=conserved;Parent=BBa_B0031
BBa_J47532	.	promoter	1	55	.	+	0	ID=BBa_R0062;Name=BBa_R0062
BBa_J47532	.	non_covalent_binding_site	1	20	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_J47532	.	start_codon	53	53	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_J47532	.	promoter	20	25	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_J47532	.	promoter	42	47	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_J47532	.	engineered_region	1	55	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
>BBa_J47532
acctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatactagagtcacacaggaaaccta
ctagatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgc
gtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattc
ccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgc
gccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacga
agcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgc
tggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagac
acccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccag
caaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactc
gcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgct
gaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgag
tccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgt
taaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggc
ggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctct
ccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacg
aaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacga
aaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggct
caccttcgggtgggcctttctgcgtttata