LOCUS       BBa_J47533              2465 bp    DNA     linear       22-Dec-2024
DEFINITION  luxR promoter + lacI inverter
ACCESSION   BBa_J47533
VERSION     BBa_J47533.1  
FEATURES             Location/Qualifiers
     misc_feature    1..20
                     /label=LuxR/HSL
     promoter        20..25
                     /label=-35
     promoter        42..47
                     /label=-10
     misc_feature    53..53
                     /label=start
     misc_feature    1..55
                     /label=BBa_R0062
     promoter        1..55
                     /label=BBa_R0062
     misc_feature    70..73
                     /label=conserved
     RBS             64..77
                     /label=BBa_B0031
     misc_feature    1173..1211
                     /label=LVA
     CDS             84..1211
                     /label=lacI-LVA
     misc_feature    84..1211
                     /label=BBa_C0012
     misc_feature    1212..1236
                     /label=Help:Barcodes
     CDS             84..1211
                     /label=BBa_C0012
     stem_loop       1256..1299
                     /label=stem_loop
     misc_feature    1245..1324
                     /label=BBa_B0010
     terminator      1245..1324
                     /label=BBa_B0010
     stem_loop       1340..1359
                     /label=T7 TE
     misc_feature    1366..1366
                     /label=stop
     polyA_site      1360..1373
                     /label=polya
     misc_feature    1333..1373
                     /label=BBa_B0012
     terminator      1333..1373
                     /label=BBa_B0012
     misc_feature    64..1373
                     /label=BBa_P0112
     misc_feature    1..1373
                     /label=BBa_J47532
     CDS             1382..1469
                     /label=end of LacI coding region (inactive)
     misc_feature    1382..1581
                     /label=BBa_R0010
     misc_feature    1470..1507
                     /label=CAP binding site
     promoter        1518..1523
                     /label=-35
     promoter        1542..1547
                     /label=-10
     misc_feature    1547..1581
                     /label=LacI binding site
     misc_feature    1554..1554
                     /label=start
     promoter        1382..1581
                     /label=BBa_R0010
     RBS             1590..1602
                     /label=RBS-3\Weak
     RBS             1596..1599
                     /label=RBS
     misc_feature    1590..1602
                     /label=BBa_B0032
     RBS             1590..1602
                     /label=BBa_B0032
     CDS             1609..2328
                     /label=GFP protein
     CDS             1609..2328
                     /label=BBa_E0040
     stem_loop       2348..2391
                     /label=stem_loop
     misc_feature    2337..2416
                     /label=BBa_B0010
     terminator      2337..2416
                     /label=BBa_B0010
     stem_loop       2432..2451
                     /label=T7 TE
     misc_feature    2458..2458
                     /label=stop
     polyA_site      2452..2465
                     /label=polya
     misc_feature    2425..2465
                     /label=BBa_B0012
     terminator      2425..2465
                     /label=BBa_B0012
     misc_feature    1382..2465
                     /label=BBa_J09264
ORIGIN
        1 acctgtagga tcgtacaggt ttacgcaaga aaatggtttg ttatagtcga ataaatacta
       61 gagtcacaca ggaaacctac tagatggtga atgtgaaacc agtaacgtta tacgatgtcg
      121 cagagtatgc cggtgtctct tatcagaccg tttcccgcgt ggtgaaccag gccagccacg
      181 tttctgcgaa aacgcgggaa aaagtggaag cggcgatggc ggagctgaat tacattccca
      241 accgcgtggc acaacaactg gcgggcaaac agtcgttgct gattggcgtt gccacctcca
      301 gtctggccct gcacgcgccg tcgcaaattg tcgcggcgat taaatctcgc gccgatcaac
      361 tgggtgccag cgtggtggtg tcgatggtag aacgaagcgg cgtcgaagcc tgtaaagcgg
      421 cggtgcacaa tcttctcgcg caacgcgtca gtgggctgat cattaactat ccgctggatg
      481 accaggatgc cattgctgtg gaagctgcct gcactaatgt tccggcgtta tttcttgatg
      541 tctctgacca gacacccatc aacagtatta ttttctccca tgaagacggt acgcgactgg
      601 gcgtggagca tctggtcgca ttgggtcacc agcaaatcgc gctgttagcg ggcccattaa
      661 gttctgtctc ggcgcgtctg cgtctggctg gctggcataa atatctcact cgcaatcaaa
      721 ttcagccgat agcggaacgg gaaggcgact ggagtgccat gtccggtttt caacaaacca
      781 tgcaaatgct gaatgagggc atcgttccca ctgcgatgct ggttgccaac gatcagatgg
      841 cgctgggcgc aatgcgcgcc attaccgagt ccgggctgcg cgttggtgcg gatatctcgg
      901 tagtgggata cgacgatacc gaagacagct catgttatat cccgccgtta accaccatca
      961 aacaggattt tcgcctgctg gggcaaacca gcgtggaccg cttgctgcaa ctctctcagg
     1021 gccaggcggt gaagggcaat cagctgttgc ccgtctcact ggtgaaaaga aaaaccaccc
     1081 tggcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
     1141 cacgacaggt ttcccgactg gaaagcgggc aggctgcaaa cgacgaaaac tacgctttag
     1201 tagcttaata actctgatag tgctagtgta gatctctact agagccaggc atcaaataaa
     1261 acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc
     1321 tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt atatactaga
     1381 gcaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
     1441 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact
     1501 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg
     1561 agcggataac aatttcacac atactagagt cacacaggaa agtactagat gcgtaaagga
     1621 gaagaacttt tcactggagt tgtcccaatt cttgttgaat tagatggtga tgttaatggg
     1681 cacaaatttt ctgtcagtgg agagggtgaa ggtgatgcaa catacggaaa acttaccctt
     1741 aaatttattt gcactactgg aaaactacct gttccatggc caacacttgt cactactttc
     1801 ggttatggtg ttcaatgctt tgcgagatac ccagatcata tgaaacagca tgactttttc
     1861 aagagtgcca tgcccgaagg ttatgtacag gaaagaacta tatttttcaa agatgacggg
     1921 aactacaaga cacgtgctga agtcaagttt gaaggtgata cccttgttaa tagaatcgag
     1981 ttaaaaggta ttgattttaa agaagatgga aacattcttg gacacaaatt ggaatacaac
     2041 tataactcac acaatgtata catcatggca gacaaacaaa agaatggaat caaagttaac
     2101 ttcaaaatta gacacaacat tgaagatgga agcgttcaac tagcagacca ttatcaacaa
     2161 aatactccaa ttggcgatgg ccctgtcctt ttaccagaca accattacct gtccacacaa
     2221 tctgcccttt cgaaagatcc caacgaaaag agagaccaca tggtccttct tgagtttgta
     2281 acagctgctg ggattacaca tggcatggat gaactataca aataataata ctagagccag
     2341 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt
     2401 gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt
     2461 ttata
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