##gff-version 3
##sequence-region BBa_J47533 1 2465
BBa_J47533	.	engineered_region	1382	2465	.	+	0	ID=BBa_J09264;Name=BBa_J09264
BBa_J47533	.	ribosome_entry_site	1590	1602	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_J09264
BBa_J47533	.	engineered_region	1590	1602	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_J47533	.	ribosome_entry_site	1590	1602	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_J47533	.	ribosome_entry_site	1596	1599	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_J47533	.	terminator	2425	2465	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_J09264
BBa_J47533	.	stop_codon	2458	2458	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J47533	.	engineered_region	2425	2465	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J47533	.	stem_loop	2432	2451	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J47533	.	polyA_site	2452	2465	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J47533	.	CDS	1609	2328	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_J09264
BBa_J47533	.	CDS	1609	2328	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_J47533	.	promoter	1382	1581	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_J09264
BBa_J47533	.	non_covalent_binding_site	1470	1507	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_J47533	.	promoter	1518	1523	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_J47533	.	engineered_region	1382	1581	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_J47533	.	CDS	1382	1469	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_J47533	.	promoter	1542	1547	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_J47533	.	start_codon	1554	1554	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_J47533	.	non_covalent_binding_site	1547	1581	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_J47533	.	terminator	2337	2416	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_J09264
BBa_J47533	.	stem_loop	2348	2391	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J47533	.	engineered_region	2337	2416	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J47533	.	engineered_region	1	1373	.	+	0	ID=BBa_J47532;Name=BBa_J47532
BBa_J47533	.	promoter	1	55	.	+	0	ID=BBa_R0062;Name=BBa_R0062;Parent=BBa_J47532
BBa_J47533	.	non_covalent_binding_site	1	20	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_J47533	.	promoter	42	47	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_J47533	.	promoter	20	25	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_J47533	.	start_codon	53	53	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_J47533	.	engineered_region	1	55	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_J47533	.	engineered_region	64	1373	.	+	0	ID=BBa_P0112;Name=BBa_P0112;Parent=BBa_J47532
BBa_J47533	.	ribosome_entry_site	64	77	.	+	0	ID=BBa_B0031;Name=BBa_B0031;Parent=BBa_P0112
BBa_J47533	.	conserved_region	70	73	.	+	0	ID=annotation23316;Name=conserved;Parent=BBa_B0031
BBa_J47533	.	terminator	1245	1324	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_P0112
BBa_J47533	.	stem_loop	1256	1299	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J47533	.	engineered_region	1245	1324	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J47533	.	CDS	84	1211	.	+	0	ID=BBa_C0012;Name=BBa_C0012;Parent=BBa_P0112
BBa_J47533	.	engineered_region	84	1211	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_J47533	.	engineered_tag	1212	1236	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_J47533	.	CDS	84	1211	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_J47533	.	tag	1173	1211	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_J47533	.	terminator	1333	1373	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_P0112
BBa_J47533	.	stop_codon	1366	1366	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J47533	.	engineered_region	1333	1373	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J47533	.	stem_loop	1340	1359	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J47533	.	polyA_site	1360	1373	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
>BBa_J47533
acctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatactagagtcacacaggaaaccta
ctagatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgc
gtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattc
ccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgc
gccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacga
agcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgc
tggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagac
acccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccag
caaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactc
gcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgct
gaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgag
tccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgt
taaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggc
ggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctct
ccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacg
aaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacga
aaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggct
caccttcgggtgggcctttctgcgtttatatactagagcaatacgcaaaccgcctctccccgcgcgttggccgattcat
taatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactc
attaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacac
atactagagtcacacaggaaagtactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaa
ttagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttaccc
ttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatg
ctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaaga
actatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaa
tcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaa
tgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagc
gttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgt
ccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgg
gattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaa
agactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtggg
cctttctgcgtttata