LOCUS       BBa_J47821              1906 bp    DNA     linear       23-May-2024
DEFINITION  HSL->GFP (AAV+)
ACCESSION   BBa_J47821
VERSION     BBa_J47821.1  
FEATURES             Location/Qualifiers
     promoter        4..9
                     /label=-35
     promoter        27..32
                     /label=-10
     misc_feature    1..38
                     /label=P(Cat)
     promoter        1..38
                     /label=BBa_I14033
     misc_feature    51..54
                     /label=conserved
     RBS             47..58
                     /label=BBa_B0034
     misc_feature    65..66
                     /label=prefix
     misc_feature    238..238
                     /label=T
     misc_feature    556..556
                     /label=A
     CDS             65..814
                     /label=luxR
     misc_feature    65..820
                     /label=BBa_C0062
     misc_feature    821..845
                     /label=Help:Barcodes
     CDS             65..820
                     /label=BBa_C0062
     stem_loop       865..908
                     /label=stem_loop
     misc_feature    854..933
                     /label=BBa_B0010
     terminator      854..933
                     /label=BBa_B0010
     stem_loop       949..968
                     /label=T7 TE
     misc_feature    975..975
                     /label=stop
     polyA_site      969..982
                     /label=polya
     misc_feature    942..982
                     /label=BBa_B0012
     terminator      942..982
                     /label=BBa_B0012
     misc_feature    47..982
                     /label=BBa_I0462
     misc_feature    1..982
                     /label=BBa_J47811
     misc_feature    997..1000
                     /label=conserved
     RBS             991..1004
                     /label=BBa_B0031
     misc_feature    1011..1769
                     /label=BBa_E0044
     stem_loop       1789..1832
                     /label=stem_loop
     misc_feature    1778..1857
                     /label=BBa_B0010
     terminator      1778..1857
                     /label=BBa_B0010
     stem_loop       1873..1892
                     /label=T7 TE
     misc_feature    1899..1899
                     /label=stop
     polyA_site      1893..1906
                     /label=polya
     misc_feature    1866..1906
                     /label=BBa_B0012
     terminator      1866..1906
                     /label=BBa_B0012
     misc_feature    991..1906
                     /label=BBa_J09144
ORIGIN
        1 ggcacgtaag aggttccaac tttcaccata atgaaacata ctagagaaag aggagaaata
       61 ctagatgaaa aacataaatg ccgacgacac atacagaata attaataaaa ttaaagcttg
      121 tagaagcaat aatgatatta atcaatgctt atctgatatg actaaaatgg tacattgtga
      181 atattattta ctcgcgatca tttatcctca ttctatggtt aaatctgata tttcaatcct
      241 agataattac cctaaaaaat ggaggcaata ttatgatgac gctaatttaa taaaatatga
      301 tcctatagta gattattcta actccaatca ttcaccaatt aattggaata tatttgaaaa
      361 caatgctgta aataaaaaat ctccaaatgt aattaaagaa gcgaaaacat caggtcttat
      421 cactgggttt agtttcccta ttcatacggc taacaatggc ttcggaatgc ttagttttgc
      481 acattcagaa aaagacaact atatagatag tttattttta catgcgtgta tgaacatacc
      541 attaattgtt ccttctctag ttgataatta tcgaaaaata aatatagcaa ataataaatc
      601 aaacaacgat ttaaccaaaa gagaaaaaga atgtttagcg tgggcatgcg aaggaaaaag
      661 ctcttgggat atttcaaaaa tattaggttg cagtgagcgt actgtcactt tccatttaac
      721 caatgcgcaa atgaaactca atacaacaaa ccgctgccaa agtatttcta aagcaatttt
      781 aacaggagca attgattgcc catactttaa aaattaataa cactgatagt gctagtgtag
      841 atcactacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt
      901 cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg
      961 ggtgggcctt tctgcgttta tatactagag tcacacagga aacctactag atgcgtaaag
     1021 gagaagaact tttcactgga gttgtcccaa ttcttgttga attagatggt gatgttaatg
     1081 ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc aacatacgga aaacttaccc
     1141 ttaaatttat ttgcactact ggaaaactac ctgttccatg gccaacactt gtcactactt
     1201 tcggttatgg tgttcaatgc tttgcgagat acccagatca tatgaaacag catgactttt
     1261 tcaagagtgc catgcccgaa ggttatgtac aggaaagaac tatatttttc aaagatgacg
     1321 ggaactacaa gacacgtgct gaagtcaagt ttgaaggtga tacccttgtt aatagaatcg
     1381 agttaaaagg tattgatttt aaagaagatg gaaacattct tggacacaaa ttggaataca
     1441 actataactc acacaatgta tacatcatgg cagacaaaca aaagaatgga atcaaagtta
     1501 acttcaaaat tagacacaac attgaagatg gaagcgttca actagcagac cattatcaac
     1561 aaaatactcc aattggcgat ggccctgtcc ttttaccaga caaccattac ctgtccacac
     1621 aatctgccct ttcgaaagat cccaacgaaa agagagacca catggtcctt cttgagtttg
     1681 taacagctgc tgggattaca catggcatgg atgaactata caaaaggcct gctgcaaacg
     1741 acgaaaacta cgctgctgct gtttaataat actagagcca ggcatcaaat aaaacgaaag
     1801 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac
     1861 tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttata
//