LOCUS       BBa_J47822              1904 bp    DNA     linear       18-May-2024
DEFINITION  HSL->GFP (AAV+)
ACCESSION   BBa_J47822
VERSION     BBa_J47822.1  
FEATURES             Location/Qualifiers
     promoter        4..9
                     /label=-35
     promoter        27..32
                     /label=-10
     misc_feature    1..38
                     /label=P(Cat)
     promoter        1..38
                     /label=BBa_I14033
     misc_feature    51..54
                     /label=conserved
     RBS             47..58
                     /label=BBa_B0034
     misc_feature    65..66
                     /label=prefix
     misc_feature    238..238
                     /label=T
     misc_feature    556..556
                     /label=A
     CDS             65..814
                     /label=luxR
     misc_feature    65..820
                     /label=BBa_C0062
     misc_feature    821..845
                     /label=Help:Barcodes
     CDS             65..820
                     /label=BBa_C0062
     stem_loop       865..908
                     /label=stem_loop
     misc_feature    854..933
                     /label=BBa_B0010
     terminator      854..933
                     /label=BBa_B0010
     stem_loop       949..968
                     /label=T7 TE
     misc_feature    975..975
                     /label=stop
     polyA_site      969..982
                     /label=polya
     misc_feature    942..982
                     /label=BBa_B0012
     terminator      942..982
                     /label=BBa_B0012
     misc_feature    47..982
                     /label=BBa_I0462
     misc_feature    1..982
                     /label=BBa_J47811
     misc_feature    995..998
                     /label=conserved
     RBS             991..1002
                     /label=BBa_B0034
     misc_feature    1009..1767
                     /label=BBa_E0044
     stem_loop       1787..1830
                     /label=stem_loop
     misc_feature    1776..1855
                     /label=BBa_B0010
     terminator      1776..1855
                     /label=BBa_B0010
     stem_loop       1871..1890
                     /label=T7 TE
     misc_feature    1897..1897
                     /label=stop
     polyA_site      1891..1904
                     /label=polya
     misc_feature    1864..1904
                     /label=BBa_B0012
     terminator      1864..1904
                     /label=BBa_B0012
     misc_feature    991..1904
                     /label=BBa_J09142
ORIGIN
        1 ggcacgtaag aggttccaac tttcaccata atgaaacata ctagagaaag aggagaaata
       61 ctagatgaaa aacataaatg ccgacgacac atacagaata attaataaaa ttaaagcttg
      121 tagaagcaat aatgatatta atcaatgctt atctgatatg actaaaatgg tacattgtga
      181 atattattta ctcgcgatca tttatcctca ttctatggtt aaatctgata tttcaatcct
      241 agataattac cctaaaaaat ggaggcaata ttatgatgac gctaatttaa taaaatatga
      301 tcctatagta gattattcta actccaatca ttcaccaatt aattggaata tatttgaaaa
      361 caatgctgta aataaaaaat ctccaaatgt aattaaagaa gcgaaaacat caggtcttat
      421 cactgggttt agtttcccta ttcatacggc taacaatggc ttcggaatgc ttagttttgc
      481 acattcagaa aaagacaact atatagatag tttattttta catgcgtgta tgaacatacc
      541 attaattgtt ccttctctag ttgataatta tcgaaaaata aatatagcaa ataataaatc
      601 aaacaacgat ttaaccaaaa gagaaaaaga atgtttagcg tgggcatgcg aaggaaaaag
      661 ctcttgggat atttcaaaaa tattaggttg cagtgagcgt actgtcactt tccatttaac
      721 caatgcgcaa atgaaactca atacaacaaa ccgctgccaa agtatttcta aagcaatttt
      781 aacaggagca attgattgcc catactttaa aaattaataa cactgatagt gctagtgtag
      841 atcactacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt
      901 cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg
      961 ggtgggcctt tctgcgttta tatactagag aaagaggaga aatactagat gcgtaaagga
     1021 gaagaacttt tcactggagt tgtcccaatt cttgttgaat tagatggtga tgttaatggg
     1081 cacaaatttt ctgtcagtgg agagggtgaa ggtgatgcaa catacggaaa acttaccctt
     1141 aaatttattt gcactactgg aaaactacct gttccatggc caacacttgt cactactttc
     1201 ggttatggtg ttcaatgctt tgcgagatac ccagatcata tgaaacagca tgactttttc
     1261 aagagtgcca tgcccgaagg ttatgtacag gaaagaacta tatttttcaa agatgacggg
     1321 aactacaaga cacgtgctga agtcaagttt gaaggtgata cccttgttaa tagaatcgag
     1381 ttaaaaggta ttgattttaa agaagatgga aacattcttg gacacaaatt ggaatacaac
     1441 tataactcac acaatgtata catcatggca gacaaacaaa agaatggaat caaagttaac
     1501 ttcaaaatta gacacaacat tgaagatgga agcgttcaac tagcagacca ttatcaacaa
     1561 aatactccaa ttggcgatgg ccctgtcctt ttaccagaca accattacct gtccacacaa
     1621 tctgcccttt cgaaagatcc caacgaaaag agagaccaca tggtccttct tgagtttgta
     1681 acagctgctg ggattacaca tggcatggat gaactataca aaaggcctgc tgcaaacgac
     1741 gaaaactacg ctgctgctgt ttaataatac tagagccagg catcaaataa aacgaaaggc
     1801 tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta
     1861 gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tata
//