LOCUS       BBa_J47861              1128 bp    DNA     linear       22-Dec-2024
DEFINITION  pLacI + ECFP (LVA+)
ACCESSION   BBa_J47861
VERSION     BBa_J47861.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     RBS             209..223
                     /label=RBS-1\Strong
     RBS             216..220
                     /label=RBS
     misc_feature    209..223
                     /label=BBa_B0030
     RBS             209..223
                     /label=BBa_B0030
     CDS             230..991
                     /label=CFP (LVA)
     misc_feature    948..985
                     /label=SsrA
     misc_feature    986..991
                     /label=2
     misc_feature    298..298
                     /label=A
     misc_feature    230..991
                     /label=BBa_E0022
     misc_feature    230..991
                     /label=BBa_E0022
     stem_loop       1011..1054
                     /label=stem_loop
     misc_feature    1000..1079
                     /label=BBa_B0010
     terminator      1000..1079
                     /label=BBa_B0010
     stem_loop       1095..1114
                     /label=T7 TE
     misc_feature    1121..1121
                     /label=stop
     polyA_site      1115..1128
                     /label=polya
     misc_feature    1088..1128
                     /label=BBa_B0012
     terminator      1088..1128
                     /label=BBa_B0012
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagat taaagaggag aaatactaga tggtgagcaa
      241 gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtgaa
      301 cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac
      361 cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac
      421 cctgacctgg ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt
      481 cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga
      541 cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat
      601 cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta
      661 caactacatc agccacaacg tctatatcac cgccgacaag cagaagaacg gcatcaaggc
      721 caacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca
      781 gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac
      841 ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt
      901 cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagaggc ctgctgcaaa
      961 cgacgaaaac tacgctttag tagcttaata atactagagc caggcatcaa ataaaacgaa
     1021 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct
     1081 actagagtca cactggctca ccttcgggtg ggcctttctg cgtttata
//