##gff-version 3
##sequence-region BBa_J47861 1 1128
BBa_J47861	.	terminator	1088	1128	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_J47861	.	stem_loop	1095	1114	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J47861	.	stop_codon	1121	1121	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J47861	.	engineered_region	1088	1128	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J47861	.	polyA_site	1115	1128	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J47861	.	promoter	1	200	.	+	0	ID=BBa_R0010;Name=BBa_R0010
BBa_J47861	.	non_covalent_binding_site	89	126	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_J47861	.	engineered_region	1	200	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_J47861	.	promoter	161	166	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_J47861	.	start_codon	173	173	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_J47861	.	non_covalent_binding_site	166	200	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_J47861	.	CDS	1	88	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_J47861	.	promoter	137	142	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_J47861	.	terminator	1000	1079	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_J47861	.	stem_loop	1011	1054	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J47861	.	engineered_region	1000	1079	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J47861	.	engineered_region	230	991	.	+	0	ID=BBa_E0022;Name=BBa_E0022
BBa_J47861	.	CDS	230	991	.	+	0	ID=annotation2150;Name=CFP (LVA);Parent=BBa_E0022
BBa_J47861	.	point_mutation	298	298	.	+	0	ID=annotation2155;Name=A;Parent=BBa_E0022
BBa_J47861	.	tag	948	985	.	+	0	ID=annotation2153;Name=SsrA;Parent=BBa_E0022
BBa_J47861	.	stop_codon	986	991	.	+	0	ID=annotation2154;Name=2;Parent=BBa_E0022
BBa_J47861	.	engineered_region	230	991	.	+	0	ID=annotation7040;Name=BBa_E0022;Parent=BBa_E0022
BBa_J47861	.	ribosome_entry_site	209	223	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_J47861	.	ribosome_entry_site	216	220	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_J47861	.	engineered_region	209	223	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_J47861	.	ribosome_entry_site	209	223	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
>BBa_J47861
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc
gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct
cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagattaaagaggagaaatactagatggtgag
caagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtgaacggccacaagttcagc
gtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccg
tgccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcagccgctaccccgaccacatgaagcagca
cgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaag
acccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacg
gcaacatcctggggcacaagctggagtacaactacatcagccacaacgtctatatcaccgccgacaagcagaagaacgg
catcaaggccaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacc
cccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacg
agaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagag
gcctgctgcaaacgacgaaaactacgctttagtagcttaataatactagagccaggcatcaaataaaacgaaaggctca
gtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcg
ggtgggcctttctgcgtttata