LOCUS       BBa_J47862              1127 bp    DNA     linear       22-Dec-2024
DEFINITION  pLacI + ECFP (LVA+)
ACCESSION   BBa_J47862
VERSION     BBa_J47862.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    215..218
                     /label=conserved
     RBS             209..222
                     /label=BBa_B0031
     CDS             229..990
                     /label=CFP (LVA)
     misc_feature    947..984
                     /label=SsrA
     misc_feature    985..990
                     /label=2
     misc_feature    297..297
                     /label=A
     misc_feature    229..990
                     /label=BBa_E0022
     misc_feature    229..990
                     /label=BBa_E0022
     stem_loop       1010..1053
                     /label=stem_loop
     misc_feature    999..1078
                     /label=BBa_B0010
     terminator      999..1078
                     /label=BBa_B0010
     stem_loop       1094..1113
                     /label=T7 TE
     misc_feature    1120..1120
                     /label=stop
     polyA_site      1114..1127
                     /label=polya
     misc_feature    1087..1127
                     /label=BBa_B0012
     terminator      1087..1127
                     /label=BBa_B0012
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagtc acacaggaaa cctactagat ggtgagcaag
      241 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtgaac
      301 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc
      361 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
      421 ctgacctggg gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc
      481 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac
      541 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
      601 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
      661 aactacatca gccacaacgt ctatatcacc gccgacaagc agaagaacgg catcaaggcc
      721 aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag
      781 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc
      841 cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
      901 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagaggcc tgctgcaaac
      961 gacgaaaact acgctttagt agcttaataa tactagagcc aggcatcaaa taaaacgaaa
     1021 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta
     1081 ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttata
//