LOCUS       BBa_J47863              1126 bp    DNA     linear       06-May-2024
DEFINITION  pLacI + ECFP (LVA+)
ACCESSION   BBa_J47863
VERSION     BBa_J47863.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     RBS             209..221
                     /label=RBS-3\Weak
     RBS             215..218
                     /label=RBS
     misc_feature    209..221
                     /label=BBa_B0032
     RBS             209..221
                     /label=BBa_B0032
     CDS             228..989
                     /label=CFP (LVA)
     misc_feature    946..983
                     /label=SsrA
     misc_feature    984..989
                     /label=2
     misc_feature    296..296
                     /label=A
     misc_feature    228..989
                     /label=BBa_E0022
     misc_feature    228..989
                     /label=BBa_E0022
     stem_loop       1009..1052
                     /label=stem_loop
     misc_feature    998..1077
                     /label=BBa_B0010
     terminator      998..1077
                     /label=BBa_B0010
     stem_loop       1093..1112
                     /label=T7 TE
     misc_feature    1119..1119
                     /label=stop
     polyA_site      1113..1126
                     /label=polya
     misc_feature    1086..1126
                     /label=BBa_B0012
     terminator      1086..1126
                     /label=BBa_B0012
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagtc acacaggaaa gtactagatg gtgagcaagg
      241 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtgaacg
      301 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc
      361 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc
      421 tgacctgggg cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct
      481 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg
      541 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg
      601 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca
      661 actacatcag ccacaacgtc tatatcaccg ccgacaagca gaagaacggc atcaaggcca
      721 acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc
      781 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc
      841 agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg
      901 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagaggcct gctgcaaacg
      961 acgaaaacta cgctttagta gcttaataat actagagcca ggcatcaaat aaaacgaaag
     1021 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac
     1081 tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttata
//