LOCUS BBa_J48100 4791 bp DNA linear 10-May-2024 DEFINITION Promoter/Met32p ACCESSION BBa_J48100 VERSION BBa_J48100.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 misc_feature 67..70 /label=conserved RBS 63..74 /label=BBa_B0034 misc_feature 81..82 /label=prefix misc_feature 254..254 /label=T misc_feature 572..572 /label=A CDS 81..830 /label=luxR misc_feature 81..836 /label=BBa_C0062 misc_feature 837..861 /label=Help:Barcodes CDS 81..836 /label=BBa_C0062 stem_loop 881..924 /label=stem_loop misc_feature 870..949 /label=BBa_B0010 terminator 870..949 /label=BBa_B0010 stem_loop 965..984 /label=T7 TE misc_feature 991..991 /label=stop polyA_site 985..998 /label=polya misc_feature 958..998 /label=BBa_B0012 terminator 958..998 /label=BBa_B0012 misc_feature 1007..1026 /label=LuxR/HSL promoter 1026..1031 /label=-35 promoter 1048..1053 /label=-10 misc_feature 1059..1059 /label=start misc_feature 1007..1061 /label=BBa_R0062 promoter 1007..1061 /label=BBa_R0062 misc_feature 1074..1077 /label=conserved RBS 1070..1081 /label=BBa_B0034 CDS 1088..1810 /label=BBa_E0030 stem_loop 1830..1873 /label=stem_loop misc_feature 1819..1898 /label=BBa_B0010 terminator 1819..1898 /label=BBa_B0010 stem_loop 1914..1933 /label=T7 TE misc_feature 1940..1940 /label=stop polyA_site 1934..1947 /label=polya misc_feature 1907..1947 /label=BBa_B0012 terminator 1907..1947 /label=BBa_B0012 promoter 3035..3165 /label=Pbad promoter 2998..3069 /label=PC misc_feature 1956..3034 /label=AraC promoter 1956..3165 /label=BBa_I0500 misc_feature 3178..3181 /label=conserved RBS 3174..3185 /label=BBa_B0034 misc_feature 3192..3194 /label=start misc_feature 3975..3980 /label=2 misc_feature 3942..3974 /label=LVA CDS 3192..3941 /label=aiiA misc_feature 3192..3980 /label=BBa_C0060 misc_feature 3981..4005 /label=Help:Barcodes CDS 3192..3980 /label=BBa_C0060 stem_loop 4025..4068 /label=stem_loop misc_feature 4014..4093 /label=BBa_B0010 terminator 4014..4093 /label=BBa_B0010 stem_loop 4109..4128 /label=T7 TE misc_feature 4135..4135 /label=stop polyA_site 4129..4142 /label=polya misc_feature 4102..4142 /label=BBa_B0012 terminator 4102..4142 /label=BBa_B0012 misc_feature 4728..4759 /label=LVA CDS 4149..4727 /label=luxI misc_feature 4149..4766 /label=BBa_C0061 misc_feature 4767..4791 /label=Help:Barcodes CDS 4149..4766 /label=BBa_C0061 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 agaaagagga gaaatactag atgaaaaaca taaatgccga cgacacatac agaataatta 121 ataaaattaa agcttgtaga agcaataatg atattaatca atgcttatct gatatgacta 181 aaatggtaca ttgtgaatat tatttactcg cgatcattta tcctcattct atggttaaat 241 ctgatatttc aatcctagat aattacccta aaaaatggag gcaatattat gatgacgcta 301 atttaataaa atatgatcct atagtagatt attctaactc caatcattca ccaattaatt 361 ggaatatatt tgaaaacaat gctgtaaata aaaaatctcc aaatgtaatt aaagaagcga 421 aaacatcagg tcttatcact gggtttagtt tccctattca tacggctaac aatggcttcg 481 gaatgcttag ttttgcacat tcagaaaaag acaactatat agatagttta tttttacatg 541 cgtgtatgaa cataccatta attgttcctt ctctagttga taattatcga aaaataaata 601 tagcaaataa taaatcaaac aacgatttaa ccaaaagaga aaaagaatgt ttagcgtggg 661 catgcgaagg aaaaagctct tgggatattt caaaaatatt aggttgcagt gagcgtactg 721 tcactttcca tttaaccaat gcgcaaatga aactcaatac aacaaaccgc tgccaaagta 781 tttctaaagc aattttaaca ggagcaattg attgcccata ctttaaaaat taataacact 841 gatagtgcta gtgtagatca ctactagagc caggcatcaa ataaaacgaa aggctcagtc 901 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca 961 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagacct gtaggatcgt 1021 acaggtttac gcaagaaaat ggtttgttat agtcgaataa atactagaga aagaggagaa 1081 atactagatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga 1141 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc 1201 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg 1261 gcccaccctc gtgaccacct tcggctacgg cctgcaatgc ttcgcccgct accccgacca 1321 catgaagctg cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac 1381 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga 1441 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct 1501 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca 1561 gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca 1621 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga 1681 caaccactac ctgagctacc agtccgccct gagcaaagac cccaacgaga agcgcgatca 1741 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta 1801 caagtaataa tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg 1861 cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac 1921 cttcgggtgg gcctttctgc gtttatatac tagagttatg acaacttgac ggctacatca 1981 ttcacttttt cttcacaacc ggcacggaac tcgctcgggc tggccccggt gcatttttta 2041 aatacccgcg agaaatagag ttgatcgtca aaaccaacat tgcgaccgac ggtggcgata 2101 ggcatccggg tggtgctcaa aagcagcttc gcctggctga tacgttggtc ctcgcgccag 2161 cttaagacgc taatccctaa ctgctggcgg aaaagatgtg acagacgcga cggcgacaag 2221 caaacatgct gtgcgacgct ggcgatatca aaattgctgt ctgccaggtg atcgctgatg 2281 tactgacaag cctcgcgtac ccgattatcc atcggtggat ggagcgactc gttaatcgct 2341 tccatgcgcc gcagtaacaa ttgctcaagc agatttatcg ccagcagctc cgaatagcgc 2401 ccttcccctt gcccggcgtt aatgatttgc ccaaacaggt cgctgaaatg cggctggtgc 2461 gcttcatccg ggcgaaagaa ccccgtattg gcaaatattg acggccagtt aagccattca 2521 tgccagtagg cgcgcggacg aaagtaaacc cactggtgat accattcgcg agcctccgga 2581 tgacgaccgt agtgatgaat ctctcctggc gggaacagca aaatatcacc cggtcggcaa 2641 acaaattctc gtccctgatt tttcaccacc ccctgaccgc gaatggtgag attgagaata 2701 taacctttca ttcccagcgg tcggtcgata aaaaaatcga gataaccgtt ggcctcaatc 2761 ggcgttaaac ccgccaccag atgggcatta aacgagtatc ccggcagcag gggatcattt 2821 tgcgcttcag ccatactttt catactcccg ccattcagag aagaaaccaa ttgtccatat 2881 tgcatcagac attgccgtca ctgcgtcttt tactggctct tctcgctaac caaaccggta 2941 accccgctta ttaaaagcat tctgtaacaa agcgggacca aagccatgac aaaaacgcgt 3001 aacaaaagtg tctataatca cggcagaaaa gtccacattg attatttgca cggcgtcaca 3061 ctttgctatg ccatagcatt tttatccata agattagcgg atcctacctg acgcttttta 3121 tcgcaactct ctactgtttc tccatacccg tttttttggg ctagctacta gagaaagagg 3181 agaaatacta gatgacagta aagaagcttt atttcgtccc agcaggtcgt tgtatgttgg 3241 atcattcgtc tgttaatagt acattaacac caggagaatt attagactta ccggtttggt 3301 gttatctttt ggagactgaa gaaggaccta ttttagtaga tacaggtatg ccagaaagtg 3361 cagttaataa tgaaggtctt tttaacggta catttgtcga agggcaggtt ttaccgaaaa 3421 tgactgaaga agatagaatc gtgaatattt taaaacgggt tggttatgag ccggaagacc 3481 ttctttatat tattagttct cacttgcatt ttgatcatgc aggaggaaat ggcgctttta 3541 taaatacacc aatcattgta cagcgtgctg aatatgaggc ggcgcagcat agcgaagaat 3601 atttgaaaga atgtatattg ccgaatttaa actacaaaat cattgaaggt gattatgaag 3661 tcgtaccagg agttcaatta ttgcatacac caggccatac tccagggcat caatcgctat 3721 taattgagac agaaaaatcc ggtcctgtat tattaacgat tgatgcatcg tatacgaaag 3781 agaattttga aaatgaagtg ccatttgcgg gatttgattc agaattagct ttatcttcaa 3841 ttaaacgttt aaaagaagtg gtgatgaaag agaagccgat tgttttcttt ggacatgata 3901 tagagcagga aaggggatgt aaagtgttcc ctgaatatat agctgcaaac gacgaaaact 3961 acgctttagt agcttaataa cgctgatagt gctagtgtag atcgctacta gagccaggca 4021 tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc 4081 ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta 4141 tatactagat gactataatg ataaaaaaat cggatttttt ggcaattcca tcggaggagt 4201 ataaaggtat tctaagtctt cgttatcaag tgtttaagca aagacttgag tgggacttag 4261 ttgtagaaaa taaccttgaa tcagatgagt atgataactc aaatgcagaa tatatttatg 4321 cttgtgatga tactgaaaat gtaagtggat gctggcgttt attacctaca acaggtgatt 4381 atatgctgaa aagtgttttt cctgaattgc ttggtcaaca gagtgctccc aaagatccta 4441 atatagtcga attaagtcgt tttgctgtag gtaaaaatag ctcaaagata aataactctg 4501 ctagtgaaat tacaatgaaa ctatttgaag ctatatataa acacgctgtt agtcaaggta 4561 ttacagaata tgtaacagta acatcaacag caatagagcg atttttaaag cgtattaaag 4621 ttccttgtca tcgtattgga gacaaagaaa ttcatgtatt aggtgatact aaatcggttg 4681 tattgtctat gcctattaat gaacagttta aaaaagcagt cttaaatgct gcaaacgacg 4741 aaaactacgc tttagtagct taataactct gatagtgcta gtgtagatct c //