##gff-version 3 ##sequence-region BBa_J48100 1 4766 BBa_J48100 . ribosome_entry_site 3174 3185 . + 0 ID=BBa_B0034;Name=BBa_B0034 BBa_J48100 . conserved_region 3178 3181 . + 0 ID=annotation23325;Name=conserved;Parent=BBa_B0034 BBa_J48100 . ribosome_entry_site 63 74 . + 0 ID=BBa_B0034;Name=BBa_B0034 BBa_J48100 . conserved_region 67 70 . + 0 ID=annotation23325;Name=conserved;Parent=BBa_B0034 BBa_J48100 . terminator 870 949 . + 0 ID=BBa_B0010;Name=BBa_B0010 BBa_J48100 . stem_loop 881 924 . + 0 ID=annotation4184;Name=stem_loop;Parent=BBa_B0010 BBa_J48100 . engineered_region 870 949 . + 0 ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010 BBa_J48100 . terminator 4014 4093 . + 0 ID=BBa_B0010;Name=BBa_B0010 BBa_J48100 . stem_loop 4025 4068 . + 0 ID=annotation4184;Name=stem_loop;Parent=BBa_B0010 BBa_J48100 . engineered_region 4014 4093 . + 0 ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010 BBa_J48100 . ribosome_entry_site 1070 1081 . + 0 ID=BBa_B0034;Name=BBa_B0034 BBa_J48100 . conserved_region 1074 1077 . + 0 ID=annotation23325;Name=conserved;Parent=BBa_B0034 BBa_J48100 . promoter 1956 3165 . + 0 ID=BBa_I0500;Name=BBa_I0500 BBa_J48100 . promoter 3035 3165 . + 0 ID=annotation1498841;Name=Pbad;Parent=BBa_I0500 BBa_J48100 . promoter 2998 3069 . + 0 ID=annotation1498842;Name=PC;Parent=BBa_I0500 BBa_J48100 . sequence_feature 1956 3034 . + 0 ID=annotation1498843;Name=AraC;Parent=BBa_I0500 BBa_J48100 . terminator 4102 4142 . + 0 ID=BBa_B0012;Name=BBa_B0012 BBa_J48100 . polyA_site 4129 4142 . + 0 ID=annotation1690;Name=polya;Parent=BBa_B0012 BBa_J48100 . stop_codon 4135 4135 . + 0 ID=annotation1687;Name=stop;Parent=BBa_B0012 BBa_J48100 . stem_loop 4109 4128 . + 0 ID=annotation1686;Name=T7 TE;Parent=BBa_B0012 BBa_J48100 . engineered_region 4102 4142 . + 0 ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012 BBa_J48100 . CDS 1088 1810 . + 0 ID=BBa_E0030;Name=BBa_E0030 BBa_J48100 . CDS 81 836 . + 0 ID=BBa_C0062;Name=BBa_C0062 BBa_J48100 . engineered_tag 837 861 . + 0 ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062 BBa_J48100 . point_mutation 254 254 . + 0 ID=annotation1764;Name=T;Parent=BBa_C0062 BBa_J48100 . engineered_region 81 836 . + 0 ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062 BBa_J48100 . engineered_region 81 82 . + 0 ID=annotation1762;Name=prefix;Parent=BBa_C0062 BBa_J48100 . point_mutation 572 572 . + 0 ID=annotation1765;Name=A;Parent=BBa_C0062 BBa_J48100 . CDS 81 830 . + 0 ID=annotation1766;Name=luxR;Parent=BBa_C0062 BBa_J48100 . promoter 1 54 . + 0 ID=BBa_R0040;Name=BBa_R0040 BBa_J48100 . non_covalent_binding_site 26 44 . + 0 ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040 BBa_J48100 . engineered_region 1 54 . + 0 ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040 BBa_J48100 . promoter 43 48 . + 0 ID=annotation1986787;Name=-10;Parent=BBa_R0040 BBa_J48100 . non_covalent_binding_site 1 19 . + 0 ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040 BBa_J48100 . promoter 20 25 . + 0 ID=annotation1986785;Name=-35;Parent=BBa_R0040 BBa_J48100 . terminator 1907 1947 . + 0 ID=BBa_B0012;Name=BBa_B0012 BBa_J48100 . polyA_site 1934 1947 . + 0 ID=annotation1690;Name=polya;Parent=BBa_B0012 BBa_J48100 . stop_codon 1940 1940 . + 0 ID=annotation1687;Name=stop;Parent=BBa_B0012 BBa_J48100 . stem_loop 1914 1933 . + 0 ID=annotation1686;Name=T7 TE;Parent=BBa_B0012 BBa_J48100 . engineered_region 1907 1947 . + 0 ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012 BBa_J48100 . promoter 1007 1061 . + 0 ID=BBa_R0062;Name=BBa_R0062 BBa_J48100 . engineered_region 1007 1061 . + 0 ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062 BBa_J48100 . start_codon 1059 1059 . + 0 ID=annotation2048;Name=start;Parent=BBa_R0062 BBa_J48100 . promoter 1048 1053 . + 0 ID=annotation2047;Name=-10;Parent=BBa_R0062 BBa_J48100 . promoter 1026 1031 . + 0 ID=annotation2046;Name=-35;Parent=BBa_R0062 BBa_J48100 . non_covalent_binding_site 1007 1026 . + 0 ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062 BBa_J48100 . CDS 3192 3980 . + 0 ID=BBa_C0060;Name=BBa_C0060 BBa_J48100 . engineered_region 3192 3980 . + 0 ID=annotation7037;Name=BBa_C0060;Parent=BBa_C0060 BBa_J48100 . engineered_tag 3981 4005 . + 0 ID=annotation2213987;Name=Help:Barcodes;Parent=BBa_C0060 BBa_J48100 . CDS 3192 3941 . + 0 ID=annotation1757;Name=aiiA;Parent=BBa_C0060 BBa_J48100 . tag 3942 3974 . + 0 ID=annotation1756;Name=LVA;Parent=BBa_C0060 BBa_J48100 . start_codon 3192 3194 . + 0 ID=annotation1754;Name=start;Parent=BBa_C0060 BBa_J48100 . stop_codon 3975 3980 . + 0 ID=annotation1755;Name=2;Parent=BBa_C0060 BBa_J48100 . terminator 1819 1898 . + 0 ID=BBa_B0010;Name=BBa_B0010 BBa_J48100 . stem_loop 1830 1873 . + 0 ID=annotation4184;Name=stem_loop;Parent=BBa_B0010 BBa_J48100 . engineered_region 1819 1898 . + 0 ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010 BBa_J48100 . terminator 958 998 . + 0 ID=BBa_B0012;Name=BBa_B0012 BBa_J48100 . polyA_site 985 998 . + 0 ID=annotation1690;Name=polya;Parent=BBa_B0012 BBa_J48100 . stop_codon 991 991 . + 0 ID=annotation1687;Name=stop;Parent=BBa_B0012 BBa_J48100 . stem_loop 965 984 . + 0 ID=annotation1686;Name=T7 TE;Parent=BBa_B0012 BBa_J48100 . engineered_region 958 998 . + 0 ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012 BBa_J48100 . CDS 4149 4766 . + 0 ID=BBa_C0061;Name=BBa_C0061 BBa_J48100 . engineered_region 4149 4766 . + 0 ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061 BBa_J48100 . CDS 4149 4727 . + 0 ID=annotation1761;Name=luxI;Parent=BBa_C0061 BBa_J48100 . engineered_tag 4767 4791 . + 0 ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061 BBa_J48100 . tag 4728 4759 . + 0 ID=annotation1760;Name=LVA;Parent=BBa_C0061 >BBa_J48100 tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatacta gatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaat caatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggtta aatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatga tcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaa tctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatg gcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacat accattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaacc aaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtg agcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagc aattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagag ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctct ctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtt tacgcaagaaaatggtttgttatagtcgaataaatactagagaaagaggagaaatactagatggtgagcaagggcgagg agctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcga gggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggccc accctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttca agtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccga ggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctg gggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtga acttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcga cggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgat cacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaataatactaga gccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctc tctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttatgacaacttgacggcta catcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaa atagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcc tggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacg gcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagc ctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagc agatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaat gcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagta ggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcct ggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatgg tgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgt taaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcata ctcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttct cgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaa caaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcattt ttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccatacccgtttttttggg ctagctactagagaaagaggagaaatactagatgacagtaaagaagctttatttcgtcccagcaggtcgttgtatgttg gatcattcgtctgttaatagtacattaacaccaggagaattattagacttaccggtttggtgttatcttttggagactg aagaaggacctattttagtagatacaggtatgccagaaagtgcagttaataatgaaggtctttttaacggtacatttgt cgaagggcaggttttaccgaaaatgactgaagaagatagaatcgtgaatattttaaaacgggttggttatgagccggaa gaccttctttatattattagttctcacttgcattttgatcatgcaggaggaaatggcgcttttataaatacaccaatca ttgtacagcgtgctgaatatgaggcggcgcagcatagcgaagaatatttgaaagaatgtatattgccgaatttaaacta caaaatcattgaaggtgattatgaagtcgtaccaggagttcaattattgcatacaccaggccatactccagggcatcaa tcgctattaattgagacagaaaaatccggtcctgtattattaacgattgatgcatcgtatacgaaagagaattttgaaa atgaagtgccatttgcgggatttgattcagaattagctttatcttcaattaaacgtttaaaagaagtggtgatgaaaga gaagccgattgttttctttggacatgatatagagcaggaaaggggatgtaaagtgttccctgaatatatagctgcaaac gacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaa acgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacact ggctcaccttcgggtgggcctttctgcgtttatatactagatgactataatgataaaaaaatcggattttttggcaatt ccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtag aaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaag tggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagt gctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgcta gtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaac atcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtatta ggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacg aaaactacgctttagtagcttaataactctgatagtgctagtgtagatctc