BBa_J48108
type: ComponentDefinition
persistent identity: BBa J48108
display id: BBa_J48108
version: 1
was generated by: iGEM to SBOL conversion
description: pUC57-Sulfur-m3
created: 2006-10-30T12:00:00Z
modified: 2015-08-31T04:08:51Z
mutable provenance: Micrococcus luteus (4698)
owned by: james
owned by: myers
mutable description: sulfur mediated, trimetallic probe for iron, nickel, vanadium
discontinued: false
dominant: false
experience: experience/None
group u list: _73_
m user id: 0
owner id: 969
owning group id: 73
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable notes: Cut with XbaI, Pst I, EcoRV, SmaI and BamHI
star: false
creator: Laina Magaya
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_I6114
component: BBa_J48109
component: BBa_J48110
component: BBa_J48107
component: BBa_J48112
component: BBa_J48111
sequence annotation: BBa_J48109
sequence annotation: BBa_I6114
sequence annotation: BBa_J48107
sequence annotation: BBa_J48110
sequence annotation: BBa_J48111
sequence annotation: BBa_J48112
sequence: https://synbiohub.org/public/igem/BBa_J48108_sequence/1

BBa_I6114
type: Component
persistent identity: component2328841
display id: component2328841
version: 1
top level: BBa_J48108
definition: BBa_I6114
access: public

BBa_J48109
type: Component
persistent identity: component2328824
display id: component2328824
version: 1
top level: BBa_J48108
definition: BBa_J48109
access: public

BBa_J48110
type: Component
persistent identity: component2328856
display id: component2328856
version: 1
top level: BBa_J48108
definition: BBa_J48110
access: public

BBa_J48107
type: Component
persistent identity: component2328842
display id: component2328842
version: 1
top level: BBa_J48108
definition: BBa_J48107
access: public

BBa_J48112
type: Component
persistent identity: component2328882
display id: component2328882
version: 1
top level: BBa_J48108
definition: BBa_J48112
access: public

BBa_J48111
type: Component
persistent identity: component2328867
display id: component2328867
version: 1
top level: BBa_J48108
definition: BBa_J48111
access: public

BBa_J48109
type: SequenceAnnotation
persistent identity: annotation2328824
display id: annotation2328824
version: 1
top level: BBa_J48108
location: https://synbiohub.org/public/igem/BBa_J48108/annotation2328824/range2328824/1
component: BBa_J48109

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type: Range
persistent identity: range2328824
display id: range2328824
version: 1
top level: BBa_J48108
direction: direction/reverse
start: 1
end: 4791
orientation: inline

BBa_I6114
type: SequenceAnnotation
persistent identity: annotation2328841
display id: annotation2328841
version: 1
top level: BBa_J48108
location: https://synbiohub.org/public/igem/BBa_J48108/annotation2328841/range2328841/1
component: BBa_I6114

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type: Range
persistent identity: range2328841
display id: range2328841
version: 1
top level: BBa_J48108
direction: direction/reverse
start: 4800
end: 5737
orientation: inline

BBa_J48107
type: SequenceAnnotation
persistent identity: annotation2328842
display id: annotation2328842
version: 1
top level: BBa_J48108
location: https://synbiohub.org/public/igem/BBa_J48108/annotation2328842/range2328842/1
component: BBa_J48107

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type: Range
persistent identity: range2328842
display id: range2328842
version: 1
top level: BBa_J48108
direction: direction/reverse
start: 5746
end: 6333
orientation: inline

BBa_J48110
type: SequenceAnnotation
persistent identity: annotation2328856
display id: annotation2328856
version: 1
top level: BBa_J48108
location: https://synbiohub.org/public/igem/BBa_J48108/annotation2328856/range2328856/1
component: BBa_J48110

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type: Range
persistent identity: range2328856
display id: range2328856
version: 1
top level: BBa_J48108
direction: direction/reverse
start: 6342
end: 7350
orientation: inline

BBa_J48111
type: SequenceAnnotation
persistent identity: annotation2328867
display id: annotation2328867
version: 1
top level: BBa_J48108
location: https://synbiohub.org/public/igem/BBa_J48108/annotation2328867/range2328867/1
component: BBa_J48111

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persistent identity: range2328867
display id: range2328867
version: 1
top level: BBa_J48108
direction: direction/reverse
start: 7359
end: 8284
orientation: inline

BBa_J48112
type: SequenceAnnotation
persistent identity: annotation2328882
display id: annotation2328882
version: 1
top level: BBa_J48108
location: https://synbiohub.org/public/igem/BBa_J48108/annotation2328882/range2328882/1
component: BBa_J48112

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type: Range
persistent identity: range2328882
display id: range2328882
version: 1
top level: BBa_J48108
direction: direction/reverse
start: 8291
end: 10106
orientation: inline

BBa_I6114
type: ComponentDefinition
persistent identity: BBa I6114
display id: BBa_I6114
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter O_H Test R2001.E0430
created: 2004-05-09T11:00:00Z
modified: 2015-08-31T04:07:44Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: -- No description --
discontinued: false
dominant: false
experience: experience/None
group u list: _11_1_
m user id: 0
owner id: 60
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: false
creator: cconboy
type: DnaRegion
role: SO:0000804
role: partType/Measurement
component: BBa_B0010
component: BBa_E0030
component: BBa_B0012
component: BBa_B0034
component: BBa_R2001
sequence annotation: BBa_R2001
sequence annotation: BBa_B0034
sequence annotation: BBa_E0030
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_I6114_sequence/1

BBa_B0010
type: Component
persistent identity: component950183
display id: component950183
version: 1
top level: BBa_I6114
definition: BBa_B0010
access: public

BBa_E0030
type: Component
persistent identity: component950178
display id: component950178
version: 1
top level: BBa_I6114
definition: eyfp
access: public

BBa_B0012
type: Component
persistent identity: component950193
display id: component950193
version: 1
top level: BBa_I6114
definition: BBa_B0012
access: public

BBa_B0034
type: Component
persistent identity: component950176
display id: component950176
version: 1
top level: BBa_I6114
definition: BBa_B0034
access: public

BBa_R2001
type: Component
persistent identity: component950171
display id: component950171
version: 1
top level: BBa_I6114
definition: Zif23
access: public

BBa_R2001
type: SequenceAnnotation
persistent identity: annotation950171
display id: annotation950171
version: 1
top level: BBa_I6114
location: https://synbiohub.org/public/igem/BBa_I6114/annotation950171/range950171/1
component: BBa_R2001

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type: Range
persistent identity: range950171
display id: range950171
version: 1
top level: BBa_I6114
direction: direction/forward
start: 1
end: 52
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation950176
display id: annotation950176
version: 1
top level: BBa_I6114
location: https://synbiohub.org/public/igem/BBa_I6114/annotation950176/range950176/1
component: BBa_B0034

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type: Range
persistent identity: range950176
display id: range950176
version: 1
top level: BBa_I6114
direction: direction/forward
start: 61
end: 72
orientation: inline

BBa_E0030
type: SequenceAnnotation
persistent identity: annotation950178
display id: annotation950178
version: 1
top level: BBa_I6114
location: https://synbiohub.org/public/igem/BBa_I6114/annotation950178/range950178/1
component: BBa_E0030

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type: Range
persistent identity: range950178
display id: range950178
version: 1
top level: BBa_I6114
direction: direction/forward
start: 79
end: 801
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation950183
display id: annotation950183
version: 1
top level: BBa_I6114
location: https://synbiohub.org/public/igem/BBa_I6114/annotation950183/range950183/1
component: BBa_B0010

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type: Range
persistent identity: range950183
display id: range950183
version: 1
top level: BBa_I6114
direction: direction/forward
start: 810
end: 889
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation950193
display id: annotation950193
version: 1
top level: BBa_I6114
location: https://synbiohub.org/public/igem/BBa_I6114/annotation950193/range950193/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I6114/annotation950193/range950193/1
type: Range
persistent identity: range950193
display id: range950193
version: 1
top level: BBa_I6114
direction: direction/forward
start: 898
end: 938
orientation: inline

BBa_J48107
type: ComponentDefinition
persistent identity: BBa J48107
display id: BBa_J48107
version: 1
was generated by: iGEM to SBOL conversion
description: UGT008-3 Promoter/Met32p
created: 2006-10-30T12:00:00Z
modified: 2015-08-31T04:08:51Z
mutable provenance: Zinc-finger DNA-binding protein, involved in regulating expression of the methionine biosynthetic genes, similar to Met31p (Saccharomyces cerevisiae)
owned by: james
owned by: myers
mutable description: sulfur Met32p promoter insert in pUC57
discontinued: false
dominant: false
experience: experience/None
group u list: _73_
m user id: 0
owner id: 969
owning group id: 73
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Cut with Xba I and Pst I
star: false
creator: Laina Magaya
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence: https://synbiohub.org/public/igem/BBa_J48107_sequence/1

BBa_J48109
type: ComponentDefinition
persistent identity: BBa J48109
display id: BBa_J48109
version: 1
was generated by: iGEM to SBOL conversion
description: lux I
created: 2006-11-03T12:00:00Z
modified: 2015-08-31T04:08:51Z
mutable provenance: Registry
owned by: james
owned by: myers
mutable description: luxI signalling
discontinued: false
dominant: false
group u list: _73_
m user id: 0
owner id: 969
owning group id: 73
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Both sequence were digested using EcoRV and BamHI
star: false
creator: Laina Magaya
type: DnaRegion
role: SO:0000804
role: partType/Signalling
component: BBa_C0061
component: BBa_I13207
sequence annotation: BBa_I13207
sequence annotation: BBa_C0061
sequence: https://synbiohub.org/public/igem/BBa_J48109_sequence/1

BBa_C0061
type: Component
persistent identity: component2313133
display id: component2313133
version: 1
top level: BBa_J48109
definition: luxI
access: public

BBa_I13207
type: Component
persistent identity: component2313131
display id: component2313131
version: 1
top level: BBa_J48109
definition: BBa_I13207
access: public

BBa_I13207
type: SequenceAnnotation
persistent identity: annotation2313131
display id: annotation2313131
version: 1
top level: BBa_J48109
location: https://synbiohub.org/public/igem/BBa_J48109/annotation2313131/range2313131/1
component: BBa_I13207

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type: Range
persistent identity: range2313131
display id: range2313131
version: 1
top level: BBa_J48109
direction: direction/reverse
start: 1
end: 4142
orientation: inline

BBa_C0061
type: SequenceAnnotation
persistent identity: annotation2313133
display id: annotation2313133
version: 1
top level: BBa_J48109
location: https://synbiohub.org/public/igem/BBa_J48109/annotation2313133/range2313133/1
component: BBa_C0061

https://synbiohub.org/public/igem/BBa_J48109/annotation2313133/range2313133/1
type: Range
persistent identity: range2313133
display id: range2313133
version: 1
top level: BBa_J48109
direction: direction/forward
start: 4149
end: 4766
orientation: inline

BBa_J48110
type: ComponentDefinition
persistent identity: BBa J48110
display id: BBa_J48110
version: 1
was generated by: iGEM to SBOL conversion
description: Fe Promoter+ mRFP1
created: 2006-11-03T12:00:00Z
modified: 2015-08-31T04:08:51Z
mutable provenance: PVAMU
owned by: james
owned by: myers
mutable description: Fe promoter sequence ligated to the part BBa_I13507
discontinued: false
dominant: false
group u list: _73_
m user id: 0
owner id: 969
owning group id: 73
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: XbaI cut Site on the Fe sequence.
star: false
creator: Laina Magaya
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_I13507
component: BBa_J48103
sequence annotation: BBa_J48103
sequence annotation: BBa_I13507
sequence: https://synbiohub.org/public/igem/BBa_J48110_sequence/1

BBa_I13507
type: Component
persistent identity: component2252307
display id: component2252307
version: 1
top level: BBa_J48110
definition: BBa_I13507
access: public

BBa_J48103
type: Component
persistent identity: component2252295
display id: component2252295
version: 1
top level: BBa_J48110
definition: BBa_J48103
access: public

BBa_J48103
type: SequenceAnnotation
persistent identity: annotation2252295
display id: annotation2252295
version: 1
top level: BBa_J48110
location: https://synbiohub.org/public/igem/BBa_J48110/annotation2252295/range2252295/1
component: BBa_J48103

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type: Range
persistent identity: range2252295
display id: range2252295
version: 1
top level: BBa_J48110
direction: direction/reverse
start: 1
end: 140
orientation: inline

BBa_I13507
type: SequenceAnnotation
persistent identity: annotation2252307
display id: annotation2252307
version: 1
top level: BBa_J48110
location: https://synbiohub.org/public/igem/BBa_J48110/annotation2252307/range2252307/1
component: BBa_I13507

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type: Range
persistent identity: range2252307
display id: range2252307
version: 1
top level: BBa_J48110
direction: direction/reverse
start: 149
end: 1009
orientation: inline

BBa_J48111
type: ComponentDefinition
persistent identity: BBa J48111
display id: BBa_J48111
version: 1
was generated by: iGEM to SBOL conversion
description: E. coli NikR
created: 2006-11-03T12:00:00Z
modified: 2015-08-31T04:08:51Z
mutable provenance: Registry+PVAMU
owned by: james
owned by: myers
mutable description: NikR promoter ligated to the EYFP BBa_E0430
discontinued: false
dominant: false
group u list: _73_
m user id: 0
owner id: 969
owning group id: 73
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Xba I cut site
star: false
creator: Laina Magaya
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
component: BBa_B0012
component: BBa_B0034
component: BBa_J48104
component: BBa_B0010
component: BBa_E0030
sequence annotation: BBa_J48104
sequence annotation: BBa_B0034
sequence annotation: BBa_E0030
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_J48111_sequence/1

BBa_B0012
type: Component
persistent identity: component1909818
display id: component1909818
version: 1
top level: BBa_J48111
definition: BBa_B0012
access: public

BBa_B0034
type: Component
persistent identity: component1909814
display id: component1909814
version: 1
top level: BBa_J48111
definition: BBa_B0034
access: public

BBa_J48104
type: Component
persistent identity: component1909812
display id: component1909812
version: 1
top level: BBa_J48111
definition: BBa_J48104
access: public

BBa_B0010
type: Component
persistent identity: component1909816
display id: component1909816
version: 1
top level: BBa_J48111
definition: BBa_B0010
access: public

BBa_E0030
type: Component
persistent identity: component1909815
display id: component1909815
version: 1
top level: BBa_J48111
definition: eyfp
access: public

BBa_J48104
type: SequenceAnnotation
persistent identity: annotation1909812
display id: annotation1909812
version: 1
top level: BBa_J48111
location: https://synbiohub.org/public/igem/BBa_J48111/annotation1909812/range1909812/1
component: BBa_J48104

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type: Range
persistent identity: range1909812
display id: range1909812
version: 1
top level: BBa_J48111
direction: direction/reverse
start: 1
end: 40
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1909814
display id: annotation1909814
version: 1
top level: BBa_J48111
location: https://synbiohub.org/public/igem/BBa_J48111/annotation1909814/range1909814/1
component: BBa_B0034

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type: Range
persistent identity: range1909814
display id: range1909814
version: 1
top level: BBa_J48111
direction: direction/reverse
start: 49
end: 60
orientation: inline

BBa_E0030
type: SequenceAnnotation
persistent identity: annotation1909815
display id: annotation1909815
version: 1
top level: BBa_J48111
location: https://synbiohub.org/public/igem/BBa_J48111/annotation1909815/range1909815/1
component: BBa_E0030

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type: Range
persistent identity: range1909815
display id: range1909815
version: 1
top level: BBa_J48111
direction: direction/reverse
start: 67
end: 789
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1909816
display id: annotation1909816
version: 1
top level: BBa_J48111
location: https://synbiohub.org/public/igem/BBa_J48111/annotation1909816/range1909816/1
component: BBa_B0010

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type: Range
persistent identity: range1909816
display id: range1909816
version: 1
top level: BBa_J48111
direction: direction/forward
start: 798
end: 877
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1909818
display id: annotation1909818
version: 1
top level: BBa_J48111
location: https://synbiohub.org/public/igem/BBa_J48111/annotation1909818/range1909818/1
component: BBa_B0012

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type: Range
persistent identity: range1909818
display id: range1909818
version: 1
top level: BBa_J48111
direction: direction/forward
start: 886
end: 926
orientation: inline

BBa_J48112
type: ComponentDefinition
persistent identity: BBa J48112
display id: BBa_J48112
version: 1
was generated by: iGEM to SBOL conversion
description: vnfH: vanadium promoter
created: 2006-11-03T12:00:00Z
modified: 2015-08-31T04:08:51Z
mutable provenance: PVAMU
owned by: james
owned by: myers
mutable description: Vanadium promoter
discontinued: false
dominant: false
group u list: _73_
m user id: 0
owner id: 969
owning group id: 73
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Cut sites for EcoRI and PstI
star: false
creator: Laina Magaya
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_J48106
component: BBa_E0022
component: BBa_B0034
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_J48106
sequence annotation: BBa_B0034
sequence annotation: BBa_E0022
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_J48112_sequence/1

BBa_J48106
type: Component
persistent identity: component1909774
display id: component1909774
version: 1
top level: BBa_J48112
definition: BBa_J48106
access: public

BBa_E0022
type: Component
persistent identity: component1909778
display id: component1909778
version: 1
top level: BBa_J48112
definition: ECFP
access: public

BBa_B0034
type: Component
persistent identity: component1909776
display id: component1909776
version: 1
top level: BBa_J48112
definition: BBa_B0034
access: public

BBa_B0012
type: Component
persistent identity: component1909784
display id: component1909784
version: 1
top level: BBa_J48112
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1909782
display id: component1909782
version: 1
top level: BBa_J48112
definition: BBa_B0010
access: public

BBa_J48106
type: SequenceAnnotation
persistent identity: annotation1909774
display id: annotation1909774
version: 1
top level: BBa_J48112
location: https://synbiohub.org/public/igem/BBa_J48112/annotation1909774/range1909774/1
component: BBa_J48106

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type: Range
persistent identity: range1909774
display id: range1909774
version: 1
top level: BBa_J48112
direction: direction/reverse
start: 1
end: 891
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1909776
display id: annotation1909776
version: 1
top level: BBa_J48112
location: https://synbiohub.org/public/igem/BBa_J48112/annotation1909776/range1909776/1
component: BBa_B0034

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type: Range
persistent identity: range1909776
display id: range1909776
version: 1
top level: BBa_J48112
direction: direction/reverse
start: 900
end: 911
orientation: inline

BBa_E0022
type: SequenceAnnotation
persistent identity: annotation1909778
display id: annotation1909778
version: 1
top level: BBa_J48112
location: https://synbiohub.org/public/igem/BBa_J48112/annotation1909778/range1909778/1
component: BBa_E0022

https://synbiohub.org/public/igem/BBa_J48112/annotation1909778/range1909778/1
type: Range
persistent identity: range1909778
display id: range1909778
version: 1
top level: BBa_J48112
direction: direction/forward
start: 918
end: 1679
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1909782
display id: annotation1909782
version: 1
top level: BBa_J48112
location: https://synbiohub.org/public/igem/BBa_J48112/annotation1909782/range1909782/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_J48112/annotation1909782/range1909782/1
type: Range
persistent identity: range1909782
display id: range1909782
version: 1
top level: BBa_J48112
direction: direction/forward
start: 1688
end: 1767
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1909784
display id: annotation1909784
version: 1
top level: BBa_J48112
location: https://synbiohub.org/public/igem/BBa_J48112/annotation1909784/range1909784/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_J48112/annotation1909784/range1909784/1
type: Range
persistent identity: range1909784
display id: range1909784
version: 1
top level: BBa_J48112
direction: direction/forward
start: 1776
end: 1816
orientation: inline

Zif23
type: ComponentDefinition
persistent identity: BBa R2001
display id: BBa_R2001
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter, Zif23 regulated, test: after
created: 2004-01-29T12:00:00Z
modified: 2015-05-08T01:14:15Z
mutable provenance: synthetic
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Promoter with standard -10 and -35 sites. A symmetric Zif23 dimer target site is after the -10 site.
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: consensus sequences based on [Jensen98].
star: true
creator: Ronny Krashinsky
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: consensus
sequence annotation: -35
sequence annotation: -10
sequence annotation: Zif23
sequence annotation: (+1)
sequence: https://synbiohub.org/public/igem/BBa_R2001_sequence/1

consensus
type: SequenceAnnotation
persistent identity: annotation318135
display id: annotation318135
version: 1
top level: Zif23
location: https://synbiohub.org/public/igem/BBa_R2001/annotation318135/range318135/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_R2001/annotation318135/range318135/1
type: Range
persistent identity: range318135
display id: range318135
version: 1
top level: Zif23
direction: direction/forward
start: 1
end: 9
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation318132
display id: annotation318132
version: 1
top level: Zif23
location: https://synbiohub.org/public/igem/BBa_R2001/annotation318132/range318132/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R2001/annotation318132/range318132/1
type: Range
persistent identity: range318132
display id: range318132
version: 1
top level: Zif23
direction: direction/forward
start: 10
end: 15
orientation: inline

consensus
type: SequenceAnnotation
persistent identity: annotation318142
display id: annotation318142
version: 1
top level: Zif23
location: https://synbiohub.org/public/igem/BBa_R2001/annotation318142/range318142/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_R2001/annotation318142/range318142/1
type: Range
persistent identity: range318142
display id: range318142
version: 1
top level: Zif23
direction: direction/forward
start: 30
end: 32
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation318133
display id: annotation318133
version: 1
top level: Zif23
location: https://synbiohub.org/public/igem/BBa_R2001/annotation318133/range318133/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R2001/annotation318133/range318133/1
type: Range
persistent identity: range318133
display id: range318133
version: 1
top level: Zif23
direction: direction/forward
start: 33
end: 38
orientation: inline

Zif23
type: SequenceAnnotation
persistent identity: annotation318136
display id: annotation318136
version: 1
top level: Zif23
location: https://synbiohub.org/public/igem/BBa_R2001/annotation318136/range318136/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R2001/annotation318136/range318136/1
type: Range
persistent identity: range318136
display id: range318136
version: 1
top level: Zif23
direction: direction/forward
start: 39
end: 52
orientation: inline

(+1)
type: SequenceAnnotation
persistent identity: annotation318134
display id: annotation318134
version: 1
top level: Zif23
location: https://synbiohub.org/public/igem/BBa_R2001/annotation318134/range318134/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_R2001/annotation318134/range318134/1
type: Range
persistent identity: range318134
display id: range318134
version: 1
top level: Zif23
direction: direction/forward
start: 45
end: 45
orientation: inline

BBa_I13207
type: ComponentDefinition
persistent identity: BBa I13207
display id: BBa_I13207
version: 1
was generated by: iGEM to SBOL conversion
description: HSL/aiiA test construct
created: 2004-07-31T11:00:00Z
modified: 2015-08-31T04:07:33Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: LuxR receiver circuit (driven by ptet) with pBad controlling the expression of aiiA. Useful for measuring the ability of aiiA (LVA+) to turn off the response of a tet driven receiver cell to exogenous HSL levels
discontinued: false
dominant: false
experience: experience/None
group u list: _11_6_
m user id: 0
owner id: 135
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: false
creator: Barry Canton
type: DnaRegion
role: SO:0000804
role: partType/Signalling
component: BBa_R0040
component: BBa_C0062
component: BBa_B0034
component: BBa_B0012
component: BBa_B0010
component: BBa_R0062
component: BBa_E0030
component: BBa_I0500
component: BBa_C0060
sequence annotation: BBa_R0040
sequence annotation: BBa_B0034
sequence annotation: BBa_C0062
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_R0062
sequence annotation: BBa_E0030
sequence annotation: BBa_I0500
sequence annotation: BBa_C0060
sequence: https://synbiohub.org/public/igem/BBa_I13207_sequence/1

BBa_R0040
type: Component
persistent identity: component1044028
display id: component1044028
version: 1
top level: BBa_I13207
definition: p(tetR)
access: public

BBa_C0062
type: Component
persistent identity: component1044051
display id: component1044051
version: 1
top level: BBa_I13207
definition: luxr
access: public

BBa_B0034
type: Component
persistent identity: component1044036
display id: component1044036
version: 1
top level: BBa_I13207
definition: BBa_B0034
access: public

BBa_B0012
type: Component
persistent identity: component1044069
display id: component1044069
version: 1
top level: BBa_I13207
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1044059
display id: component1044059
version: 1
top level: BBa_I13207
definition: BBa_B0010
access: public

BBa_B0034
type: Component
persistent identity: component1044092
display id: component1044092
version: 1
top level: BBa_I13207
definition: BBa_B0034
access: public

BBa_R0062
type: Component
persistent identity: component1044084
display id: component1044084
version: 1
top level: BBa_I13207
definition: lux pR
access: public

BBa_B0010
type: Component
persistent identity: component1044099
display id: component1044099
version: 1
top level: BBa_I13207
definition: BBa_B0010
access: public

BBa_E0030
type: Component
persistent identity: component1044094
display id: component1044094
version: 1
top level: BBa_I13207
definition: eyfp
access: public

BBa_I0500
type: Component
persistent identity: component1044114
display id: component1044114
version: 1
top level: BBa_I13207
definition: pBad/araC
access: public

BBa_B0012
type: Component
persistent identity: component1044109
display id: component1044109
version: 1
top level: BBa_I13207
definition: BBa_B0012
access: public

BBa_C0060
type: Component
persistent identity: component1044134
display id: component1044134
version: 1
top level: BBa_I13207
definition: aiiA
access: public

BBa_B0034
type: Component
persistent identity: component1044119
display id: component1044119
version: 1
top level: BBa_I13207
definition: BBa_B0034
access: public

BBa_B0012
type: Component
persistent identity: component1044152
display id: component1044152
version: 1
top level: BBa_I13207
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1044142
display id: component1044142
version: 1
top level: BBa_I13207
definition: BBa_B0010
access: public

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1044028
display id: annotation1044028
version: 1
top level: BBa_I13207
location: https://synbiohub.org/public/igem/BBa_I13207/annotation1044028/range1044028/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_I13207/annotation1044028/range1044028/1
type: Range
persistent identity: range1044028
display id: range1044028
version: 1
top level: BBa_I13207
direction: direction/forward
start: 1
end: 54
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1044036
display id: annotation1044036
version: 1
top level: BBa_I13207
location: https://synbiohub.org/public/igem/BBa_I13207/annotation1044036/range1044036/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13207/annotation1044036/range1044036/1
type: Range
persistent identity: range1044036
display id: range1044036
version: 1
top level: BBa_I13207
direction: direction/forward
start: 63
end: 74
orientation: inline

BBa_C0062
type: SequenceAnnotation
persistent identity: annotation1044051
display id: annotation1044051
version: 1
top level: BBa_I13207
location: https://synbiohub.org/public/igem/BBa_I13207/annotation1044051/range1044051/1
component: BBa_C0062

https://synbiohub.org/public/igem/BBa_I13207/annotation1044051/range1044051/1
type: Range
persistent identity: range1044051
display id: range1044051
version: 1
top level: BBa_I13207
direction: direction/forward
start: 81
end: 836
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1044059
display id: annotation1044059
version: 1
top level: BBa_I13207
location: https://synbiohub.org/public/igem/BBa_I13207/annotation1044059/range1044059/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I13207/annotation1044059/range1044059/1
type: Range
persistent identity: range1044059
display id: range1044059
version: 1
top level: BBa_I13207
direction: direction/forward
start: 870
end: 949
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1044069
display id: annotation1044069
version: 1
top level: BBa_I13207
location: https://synbiohub.org/public/igem/BBa_I13207/annotation1044069/range1044069/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I13207/annotation1044069/range1044069/1
type: Range
persistent identity: range1044069
display id: range1044069
version: 1
top level: BBa_I13207
direction: direction/forward
start: 958
end: 998
orientation: inline

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation1044084
display id: annotation1044084
version: 1
top level: BBa_I13207
location: https://synbiohub.org/public/igem/BBa_I13207/annotation1044084/range1044084/1
component: BBa_R0062

https://synbiohub.org/public/igem/BBa_I13207/annotation1044084/range1044084/1
type: Range
persistent identity: range1044084
display id: range1044084
version: 1
top level: BBa_I13207
direction: direction/forward
start: 1007
end: 1061
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1044092
display id: annotation1044092
version: 1
top level: BBa_I13207
location: https://synbiohub.org/public/igem/BBa_I13207/annotation1044092/range1044092/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13207/annotation1044092/range1044092/1
type: Range
persistent identity: range1044092
display id: range1044092
version: 1
top level: BBa_I13207
direction: direction/forward
start: 1070
end: 1081
orientation: inline

BBa_E0030
type: SequenceAnnotation
persistent identity: annotation1044094
display id: annotation1044094
version: 1
top level: BBa_I13207
location: https://synbiohub.org/public/igem/BBa_I13207/annotation1044094/range1044094/1
component: BBa_E0030

https://synbiohub.org/public/igem/BBa_I13207/annotation1044094/range1044094/1
type: Range
persistent identity: range1044094
display id: range1044094
version: 1
top level: BBa_I13207
direction: direction/forward
start: 1088
end: 1810
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1044099
display id: annotation1044099
version: 1
top level: BBa_I13207
location: https://synbiohub.org/public/igem/BBa_I13207/annotation1044099/range1044099/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I13207/annotation1044099/range1044099/1
type: Range
persistent identity: range1044099
display id: range1044099
version: 1
top level: BBa_I13207
direction: direction/forward
start: 1819
end: 1898
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1044109
display id: annotation1044109
version: 1
top level: BBa_I13207
location: https://synbiohub.org/public/igem/BBa_I13207/annotation1044109/range1044109/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I13207/annotation1044109/range1044109/1
type: Range
persistent identity: range1044109
display id: range1044109
version: 1
top level: BBa_I13207
direction: direction/forward
start: 1907
end: 1947
orientation: inline

BBa_I0500
type: SequenceAnnotation
persistent identity: annotation1044114
display id: annotation1044114
version: 1
top level: BBa_I13207
location: https://synbiohub.org/public/igem/BBa_I13207/annotation1044114/range1044114/1
component: BBa_I0500

https://synbiohub.org/public/igem/BBa_I13207/annotation1044114/range1044114/1
type: Range
persistent identity: range1044114
display id: range1044114
version: 1
top level: BBa_I13207
direction: direction/forward
start: 1956
end: 3165
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1044119
display id: annotation1044119
version: 1
top level: BBa_I13207
location: https://synbiohub.org/public/igem/BBa_I13207/annotation1044119/range1044119/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13207/annotation1044119/range1044119/1
type: Range
persistent identity: range1044119
display id: range1044119
version: 1
top level: BBa_I13207
direction: direction/forward
start: 3174
end: 3185
orientation: inline

BBa_C0060
type: SequenceAnnotation
persistent identity: annotation1044134
display id: annotation1044134
version: 1
top level: BBa_I13207
location: https://synbiohub.org/public/igem/BBa_I13207/annotation1044134/range1044134/1
component: BBa_C0060

https://synbiohub.org/public/igem/BBa_I13207/annotation1044134/range1044134/1
type: Range
persistent identity: range1044134
display id: range1044134
version: 1
top level: BBa_I13207
direction: direction/forward
start: 3192
end: 3980
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1044142
display id: annotation1044142
version: 1
top level: BBa_I13207
location: https://synbiohub.org/public/igem/BBa_I13207/annotation1044142/range1044142/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I13207/annotation1044142/range1044142/1
type: Range
persistent identity: range1044142
display id: range1044142
version: 1
top level: BBa_I13207
direction: direction/forward
start: 4014
end: 4093
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1044152
display id: annotation1044152
version: 1
top level: BBa_I13207
location: https://synbiohub.org/public/igem/BBa_I13207/annotation1044152/range1044152/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I13207/annotation1044152/range1044152/1
type: Range
persistent identity: range1044152
display id: range1044152
version: 1
top level: BBa_I13207
direction: direction/forward
start: 4102
end: 4142
orientation: inline

BBa_J48103
type: ComponentDefinition
persistent identity: BBa J48103
display id: BBa_J48103
version: 1
was generated by: iGEM to SBOL conversion
description: Iron promoter
created: 2006-10-30T12:00:00Z
modified: 2015-08-31T04:08:51Z
mutable provenance: Genomic
owned by: james
owned by: myers
mutable description: Encodes for oxidoreductase and promotes the reduction/oxidation of iron (FeII and FeII).
discontinued: false
dominant: false
group u list: _73_
m user id: 0
owner id: 969
owning group id: 73
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Digested using Pst I, Xba I inserted into BBa_E0422.
star: false
creator: Laina Magaya
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence: https://synbiohub.org/public/igem/BBa_J48103_sequence/1

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

eyfp
type: ComponentDefinition
persistent identity: BBa E0030
display id: BBa_E0030
version: 1
was generated by: iGEM to SBOL conversion
description: enhanced yellow fluorescent protein derived from A. victoria GFP
created: 2004-03-02T12:00:00Z
modified: 2015-08-31T04:07:25Z
mutable provenance: Modificaitons to Clontech EYFP by Reshma Shetty
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: -- No description --
discontinued: false
dominant: false
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Caitlin Conboy and Jennifer Braff
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_E0030_sequence/1

luxI
type: ComponentDefinition
persistent identity: BBa C0061
display id: BBa_C0061
version: 1
was generated by: iGEM to SBOL conversion
description: autoinducer synthetase for AHL
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: V. fischeri <genbank>AF170104</genbank>
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Synthesizes 3OC6HSL, which binds to LuxR. The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the Lux repressor, LuxR. Two molecules of LuxR protein form a complex with two molecules the signalling compound HSL. This complex binds to a palindromic site on the promoter, increasing the rate of transcription.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: An LVA tail (sequence: AANDENYALVA) was added to increase protein degradation. .
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: BBa_C0061
sequence annotation: luxI
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0061_sequence/1

BBa_C0061
type: SequenceAnnotation
persistent identity: annotation7038
display id: annotation7038
version: 1
top level: luxI
location: https://synbiohub.org/public/igem/BBa_C0061/annotation7038/range7038/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_C0061/annotation7038/range7038/1
type: Range
persistent identity: range7038
display id: range7038
version: 1
top level: luxI
direction: direction/forward
start: 1
end: 618
orientation: inline

luxI
type: SequenceAnnotation
persistent identity: annotation1761
display id: annotation1761
version: 1
top level: luxI
location: https://synbiohub.org/public/igem/BBa_C0061/annotation1761/range1761/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_C0061/annotation1761/range1761/1
type: Range
persistent identity: range1761
display id: range1761
version: 1
top level: luxI
direction: direction/forward
start: 1
end: 579
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation1760
display id: annotation1760
version: 1
top level: luxI
location: https://synbiohub.org/public/igem/BBa_C0061/annotation1760/range1760/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_C0061/annotation1760/range1760/1
type: Range
persistent identity: range1760
display id: range1760
version: 1
top level: luxI
direction: direction/forward
start: 580
end: 611
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213985
display id: annotation2213985
version: 1
top level: luxI
location: https://synbiohub.org/public/igem/BBa_C0061/annotation2213985/range2213985/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_C0061/annotation2213985/range2213985/1
type: Range
persistent identity: range2213985
display id: range2213985
version: 1
top level: luxI
direction: direction/forward
start: 619
end: 643
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

BBa_J48104
type: ComponentDefinition
persistent identity: BBa J48104
display id: BBa_J48104
version: 1
was generated by: iGEM to SBOL conversion
description: NikR promoter, a protein of the ribbon helix-helix family of trancription factors that repress expre
created: 2006-10-30T12:00:00Z
modified: 2015-08-31T04:08:51Z
mutable provenance: Escherichia coli UTI89
owned by: james
owned by: myers
mutable description: Nickel responsive transcriptional regulator
discontinued: false
dominant: false
experience: experience/None
group u list: _73_
m user id: 0
owner id: 969
owning group id: 73
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Cut with Pst I and Xba I
star: false
creator: Laina Magaya
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J48104_sequence/1

BBa_J48106
type: ComponentDefinition
persistent identity: BBa J48106
display id: BBa_J48106
version: 1
was generated by: iGEM to SBOL conversion
description: vnfH
created: 2006-10-30T12:00:00Z
modified: 2015-08-31T04:08:51Z
mutable provenance: nitrogenase iron protein, vnfH Rhodopseudomonas palustris CGA009
owned by: james
owned by: myers
mutable description: Vanadium promoter
discontinued: false
dominant: false
group u list: _73_
m user id: 0
owner id: 969
owning group id: 73
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: cut with Xba I and Pst I
star: false
creator: Laina Magaya
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence: https://synbiohub.org/public/igem/BBa_J48106_sequence/1

BBa_I13507
type: ComponentDefinition
persistent identity: BBa I13507
display id: BBa_I13507
version: 1
was generated by: iGEM to SBOL conversion
description: Screening plasmid intermediate
created: 2005-05-30T11:00:00Z
modified: 2015-08-31T04:07:34Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Built by Josh as an intermediate in screening plasmid construction.
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jkm
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_B0034
component: BBa_B0010
component: BBa_E1010
component: BBa_B0012
sequence annotation: BBa_B0034
sequence annotation: BBa_E1010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_I13507_sequence/1

BBa_B0034
type: Component
persistent identity: component1524815
display id: component1524815
version: 1
top level: BBa_I13507
definition: BBa_B0034
access: public

BBa_B0010
type: Component
persistent identity: component1524828
display id: component1524828
version: 1
top level: BBa_I13507
definition: BBa_B0010
access: public

BBa_E1010
type: Component
persistent identity: component1524822
display id: component1524822
version: 1
top level: BBa_I13507
definition: mRFP1
access: public

BBa_B0012
type: Component
persistent identity: component1524838
display id: component1524838
version: 1
top level: BBa_I13507
definition: BBa_B0012
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1524815
display id: annotation1524815
version: 1
top level: BBa_I13507
location: https://synbiohub.org/public/igem/BBa_I13507/annotation1524815/range1524815/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I13507/annotation1524815/range1524815/1
type: Range
persistent identity: range1524815
display id: range1524815
version: 1
top level: BBa_I13507
direction: direction/forward
start: 1
end: 12
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation1524822
display id: annotation1524822
version: 1
top level: BBa_I13507
location: https://synbiohub.org/public/igem/BBa_I13507/annotation1524822/range1524822/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_I13507/annotation1524822/range1524822/1
type: Range
persistent identity: range1524822
display id: range1524822
version: 1
top level: BBa_I13507
direction: direction/forward
start: 19
end: 699
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1524828
display id: annotation1524828
version: 1
top level: BBa_I13507
location: https://synbiohub.org/public/igem/BBa_I13507/annotation1524828/range1524828/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I13507/annotation1524828/range1524828/1
type: Range
persistent identity: range1524828
display id: range1524828
version: 1
top level: BBa_I13507
direction: direction/forward
start: 733
end: 812
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1524838
display id: annotation1524838
version: 1
top level: BBa_I13507
location: https://synbiohub.org/public/igem/BBa_I13507/annotation1524838/range1524838/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I13507/annotation1524838/range1524838/1
type: Range
persistent identity: range1524838
display id: range1524838
version: 1
top level: BBa_I13507
direction: direction/forward
start: 821
end: 861
orientation: inline

ECFP
type: ComponentDefinition
persistent identity: BBa E0022
display id: BBa_E0022
version: 1
was generated by: iGEM to SBOL conversion
description: enhanced cyan fluorescent protein derived from A. victoria GFP
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:25Z
mutable provenance: Modified from <bb_part>BBa_E0021</bb_part>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Cyan fluorescent protein (ECFP) reporter coding sequence without the Ribosome Binding Site. Modified with an LVA tail for rapid degradation of the protein and faster fall time for the emission.
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: BBa_E0022 cyan fluorescent protein is based on BioBrick part BBa_E0021. It has been modified to include a rapid degradation LVA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: BBa_E0022
sequence annotation: CFP (LVA)
sequence annotation: A
sequence annotation: SsrA
sequence annotation: 2
sequence: https://synbiohub.org/public/igem/BBa_E0022_sequence/1

BBa_E0022
type: SequenceAnnotation
persistent identity: annotation7040
display id: annotation7040
version: 1
top level: ECFP
location: https://synbiohub.org/public/igem/BBa_E0022/annotation7040/range7040/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_E0022/annotation7040/range7040/1
type: Range
persistent identity: range7040
display id: range7040
version: 1
top level: ECFP
direction: direction/forward
start: 1
end: 762
orientation: inline

CFP (LVA)
type: SequenceAnnotation
persistent identity: annotation2150
display id: annotation2150
version: 1
top level: ECFP
location: https://synbiohub.org/public/igem/BBa_E0022/annotation2150/range2150/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_E0022/annotation2150/range2150/1
type: Range
persistent identity: range2150
display id: range2150
version: 1
top level: ECFP
direction: direction/forward
start: 1
end: 762
orientation: inline

A
type: SequenceAnnotation
persistent identity: annotation2155
display id: annotation2155
version: 1
top level: ECFP
location: https://synbiohub.org/public/igem/BBa_E0022/annotation2155/range2155/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_E0022/annotation2155/range2155/1
type: Range
persistent identity: range2155
display id: range2155
version: 1
top level: ECFP
direction: direction/forward
start: 69
end: 69
orientation: inline

SsrA
type: SequenceAnnotation
persistent identity: annotation2153
display id: annotation2153
version: 1
top level: ECFP
location: https://synbiohub.org/public/igem/BBa_E0022/annotation2153/range2153/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_E0022/annotation2153/range2153/1
type: Range
persistent identity: range2153
display id: range2153
version: 1
top level: ECFP
direction: direction/forward
start: 719
end: 756
orientation: inline

2
type: SequenceAnnotation
persistent identity: annotation2154
display id: annotation2154
version: 1
top level: ECFP
location: https://synbiohub.org/public/igem/BBa_E0022/annotation2154/range2154/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_E0022/annotation2154/range2154/1
type: Range
persistent identity: range2154
display id: range2154
version: 1
top level: ECFP
direction: direction/forward
start: 757
end: 762
orientation: inline

p(tetR)
type: ComponentDefinition
persistent identity: BBa R0040
display id: BBa_R0040
version: 1
was generated by: iGEM to SBOL conversion
description: TetR repressible promoter
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: Lutz, R., Bujard, H., Nucleic Acids Research (1997) 25, 1203-1210.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Sequence for pTet inverting regulator driven by the TetR protein.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0040
sequence annotation: TetR 1
sequence annotation: -35
sequence annotation: TetR 2
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0040_sequence/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1986784
display id: annotation1986784
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
type: Range
persistent identity: range1986784
display id: range1986784
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 54
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation1986783
display id: annotation1986783
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
type: Range
persistent identity: range1986783
display id: range1986783
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1986785
display id: annotation1986785
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
type: Range
persistent identity: range1986785
display id: range1986785
version: 1
top level: p(tetR)
direction: direction/forward
start: 20
end: 25
orientation: inline

TetR 2
type: SequenceAnnotation
persistent identity: annotation1986786
display id: annotation1986786
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
type: Range
persistent identity: range1986786
display id: range1986786
version: 1
top level: p(tetR)
direction: direction/forward
start: 26
end: 44
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1986787
display id: annotation1986787
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
type: Range
persistent identity: range1986787
display id: range1986787
version: 1
top level: p(tetR)
direction: direction/forward
start: 43
end: 48
orientation: inline

luxr
type: ComponentDefinition
persistent identity: BBa C0062
display id: BBa_C0062
version: 1
was generated by: iGEM to SBOL conversion
description: luxR repressor/activator, (no LVA?)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: V. fischeri <genbank>AF170104</genbank>
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: In complex with HSL, LuxR binds to the Lux promoter, activating transcription from Pr <bb_part>BBa_R0062</bb_part>, and repressing transcription from Pl <bb_part>BBa_R0063</bb_part>. The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the Lux activator, LuxR complexed to HSL. Two molecules of LuxR protein form a complex with two molecules the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription.
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: 2 silent point mutants were introduced in the coding sequence to remove internal XbaI and PstI sites. Mutation sites were chosen to replace codons commonly used in E. coli with codons used at a similar frequency.
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: luxR
sequence annotation: prefix
sequence annotation: BBa_C0062
sequence annotation: T
sequence annotation: A
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0062_sequence/1

luxR
type: SequenceAnnotation
persistent identity: annotation1766
display id: annotation1766
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1766/range1766/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_C0062/annotation1766/range1766/1
type: Range
persistent identity: range1766
display id: range1766
version: 1
top level: luxr
direction: direction/forward
start: 1
end: 750
orientation: inline

prefix
type: SequenceAnnotation
persistent identity: annotation1762
display id: annotation1762
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1762/range1762/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_C0062/annotation1762/range1762/1
type: Range
persistent identity: range1762
display id: range1762
version: 1
top level: luxr
direction: direction/forward
start: 1
end: 2
orientation: inline

BBa_C0062
type: SequenceAnnotation
persistent identity: annotation7039
display id: annotation7039
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation7039/range7039/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_C0062/annotation7039/range7039/1
type: Range
persistent identity: range7039
display id: range7039
version: 1
top level: luxr
direction: direction/forward
start: 1
end: 756
orientation: inline

T
type: SequenceAnnotation
persistent identity: annotation1764
display id: annotation1764
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1764/range1764/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_C0062/annotation1764/range1764/1
type: Range
persistent identity: range1764
display id: range1764
version: 1
top level: luxr
direction: direction/forward
start: 174
end: 174
orientation: inline

A
type: SequenceAnnotation
persistent identity: annotation1765
display id: annotation1765
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1765/range1765/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_C0062/annotation1765/range1765/1
type: Range
persistent identity: range1765
display id: range1765
version: 1
top level: luxr
direction: direction/forward
start: 492
end: 492
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213986
display id: annotation2213986
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation2213986/range2213986/1
role: feature/barcode
role: SO:0000807

https://synbiohub.org/public/igem/BBa_C0062/annotation2213986/range2213986/1
type: Range
persistent identity: range2213986
display id: range2213986
version: 1
top level: luxr
direction: direction/forward
start: 757
end: 781
orientation: inline

lux pR
type: ComponentDefinition
persistent identity: BBa R0062
display id: BBa_R0062
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (luxR & HSL regulated -- lux pR)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:15Z
mutable provenance: V. fischeri
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Promoter activated by LuxR in concert with HSL The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the LuxR activator protein complexed with the autoinducer, 3-oxo-hexanoyl-HSL. Two molecules of LuxR protein form a complex with two molecules of the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription.
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: This promoter is based on the Vibrio fischeri quorum sensing gene promoters. Two genes LuxI and LuxR and transcribed in opposite directions as shown below. The original sequence from which the parts <bb_part>BBa_R0062</bb_part> and <bb_part>BBa_R0063</bb_part> were derived is shown in the picture below. <img src="<bb_file>Image1.gif</bb_file>" width="614" height="362">
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: BBa_R0062
sequence annotation: LuxR/HSL
sequence annotation: -35
sequence annotation: -10
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_R0062_sequence/1

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation7070
display id: annotation7070
version: 1
top level: lux pR
location: https://synbiohub.org/public/igem/BBa_R0062/annotation7070/range7070/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0062/annotation7070/range7070/1
type: Range
persistent identity: range7070
display id: range7070
version: 1
top level: lux pR
direction: direction/forward
start: 1
end: 55
orientation: inline

LuxR/HSL
type: SequenceAnnotation
persistent identity: annotation2045
display id: annotation2045
version: 1
top level: lux pR
location: https://synbiohub.org/public/igem/BBa_R0062/annotation2045/range2045/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0062/annotation2045/range2045/1
type: Range
persistent identity: range2045
display id: range2045
version: 1
top level: lux pR
direction: direction/forward
start: 1
end: 20
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2046
display id: annotation2046
version: 1
top level: lux pR
location: https://synbiohub.org/public/igem/BBa_R0062/annotation2046/range2046/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0062/annotation2046/range2046/1
type: Range
persistent identity: range2046
display id: range2046
version: 1
top level: lux pR
direction: direction/forward
start: 20
end: 25
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2047
display id: annotation2047
version: 1
top level: lux pR
location: https://synbiohub.org/public/igem/BBa_R0062/annotation2047/range2047/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0062/annotation2047/range2047/1
type: Range
persistent identity: range2047
display id: range2047
version: 1
top level: lux pR
direction: direction/forward
start: 42
end: 47
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2048
display id: annotation2048
version: 1
top level: lux pR
location: https://synbiohub.org/public/igem/BBa_R0062/annotation2048/range2048/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_R0062/annotation2048/range2048/1
type: Range
persistent identity: range2048
display id: range2048
version: 1
top level: lux pR
direction: direction/forward
start: 53
end: 53
orientation: inline

pBad/araC
type: ComponentDefinition
persistent identity: BBa I0500
display id: BBa_I0500
version: 1
was generated by: iGEM to SBOL conversion
description: Inducible pBad/araC promoter
created: 2003-12-05T12:00:00Z
modified: 2015-08-31T04:07:29Z
owned by: james
owned by: myers
mutable description: -- No description --
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Sri Kosuri
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: AraC
sequence annotation: PC
sequence annotation: Pbad
sequence: https://synbiohub.org/public/igem/BBa_I0500_sequence/1

AraC
type: SequenceAnnotation
persistent identity: annotation1498843
display id: annotation1498843
version: 1
top level: pBad/araC
location: https://synbiohub.org/public/igem/BBa_I0500/annotation1498843/range1498843/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_I0500/annotation1498843/range1498843/1
type: Range
persistent identity: range1498843
display id: range1498843
version: 1
top level: pBad/araC
direction: direction/forward
start: 1
end: 1079
orientation: inline

PC
type: SequenceAnnotation
persistent identity: annotation1498842
display id: annotation1498842
version: 1
top level: pBad/araC
location: https://synbiohub.org/public/igem/BBa_I0500/annotation1498842/range1498842/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_I0500/annotation1498842/range1498842/1
type: Range
persistent identity: range1498842
display id: range1498842
version: 1
top level: pBad/araC
direction: direction/forward
start: 1043
end: 1114
orientation: inline

Pbad
type: SequenceAnnotation
persistent identity: annotation1498841
display id: annotation1498841
version: 1
top level: pBad/araC
location: https://synbiohub.org/public/igem/BBa_I0500/annotation1498841/range1498841/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_I0500/annotation1498841/range1498841/1
type: Range
persistent identity: range1498841
display id: range1498841
version: 1
top level: pBad/araC
direction: direction/forward
start: 1080
end: 1210
orientation: inline

aiiA
type: ComponentDefinition
persistent identity: BBa C0060
display id: BBa_C0060
version: 1
was generated by: iGEM to SBOL conversion
description: autoinducer inactivation enzyme from Bacillus; hydrolyzes acetyl homoserine lactone
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: <genbank>AF196486</genbank> from Bacillus sp. 240B1 putative metallohydrolase (aiiA) gene. Dong,Y.H., Xu,J.L., Li,X.Z. and Zhang,L.H.: AiiA, an enzyme that inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of Erwinia carotovora, Proc. Natl. Acad. Sci. U.S.A. 97 (7), 3526-3531 (2000).
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Coding region for the autoinducer inactivation enzyme A (aiiA) LVA tagged. The gene was originally isolated from Bacillus sp. 240B1 and it encodes an enzyme that catalyzes the degradation of N-acyl homoserine lactones (AHLs)--quorum sensing autoinducers.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: References (unparsed) here: Dong,Y.H., Xu,J.L., Li,X.Z. and Zhang,L.H.: AiiA, an enzyme that inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of Erwinia carotovora, Proc. Natl. Acad. Sci. U.S.A. 97 (7), 3526-3531 (2000). http://www.pnas.org/cgi/content/full/97/7/3526 Lee SJ, Park SY, Lee JJ, Yum DY, Koo BT, Lee JK.: Genes encoding the N-acyl homoserine lactone-degrading enzyme are widespread in many subspecies of Bacillus thuringiensis, Appl Environ Microbiol 2002 Aug;68(8):3919-24. http://aem.asm.org/cgi/content/full/68/8/3919?view=full&pmid=12147491 References (unparsed) here: Dong,Y.H., Xu,J.L., Li,X.Z. and Zhang,L.H.: AiiA, an enzyme that inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of Erwinia carotovora, Proc. Natl. Acad. Sci. U.S.A. 97 (7), 3526-3531 (2000). http://www.pnas.org/cgi/content/full/97/7/3526 Lee SJ, Park SY, Lee JJ, Yum DY, Koo BT, Lee JK.: Genes encoding the N-acyl homoserine lactone-degrading enzyme are widespread in many subspecies of Bacillus thuringiensis, Appl Environ Microbiol 2002 Aug;68(8):3919-24. http://aem.asm.org/cgi/content/full/68/8/3919?view=full&pmid=12147491 BBa_C0060 insert contains open reading frame (nucleotides 49-801) of the GeneBank sequence AF196486 followed by the LVA tag and two double stop codons inserted in the BioBrick prefix and suffix flanking regions. The original stop codon was TAG and in the present sequence it was substituted by TAATAA.
star: true
creator: Vinay S Mahajan, Brian Chow, Peter Carr, Voichita D. Marinescu and Alexander D. Wissner-Gross
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: aiiA
sequence annotation: BBa_C0060
sequence annotation: start
sequence annotation: LVA
sequence annotation: 2
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0060_sequence/1

aiiA
type: SequenceAnnotation
persistent identity: annotation1757
display id: annotation1757
version: 1
top level: aiiA
location: https://synbiohub.org/public/igem/BBa_C0060/annotation1757/range1757/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_C0060/annotation1757/range1757/1
type: Range
persistent identity: range1757
display id: range1757
version: 1
top level: aiiA
direction: direction/forward
start: 1
end: 750
orientation: inline

BBa_C0060
type: SequenceAnnotation
persistent identity: annotation7037
display id: annotation7037
version: 1
top level: aiiA
location: https://synbiohub.org/public/igem/BBa_C0060/annotation7037/range7037/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_C0060/annotation7037/range7037/1
type: Range
persistent identity: range7037
display id: range7037
version: 1
top level: aiiA
direction: direction/forward
start: 1
end: 789
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1754
display id: annotation1754
version: 1
top level: aiiA
location: https://synbiohub.org/public/igem/BBa_C0060/annotation1754/range1754/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_C0060/annotation1754/range1754/1
type: Range
persistent identity: range1754
display id: range1754
version: 1
top level: aiiA
direction: direction/forward
start: 1
end: 3
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation1756
display id: annotation1756
version: 1
top level: aiiA
location: https://synbiohub.org/public/igem/BBa_C0060/annotation1756/range1756/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_C0060/annotation1756/range1756/1
type: Range
persistent identity: range1756
display id: range1756
version: 1
top level: aiiA
direction: direction/forward
start: 751
end: 783
orientation: inline

2
type: SequenceAnnotation
persistent identity: annotation1755
display id: annotation1755
version: 1
top level: aiiA
location: https://synbiohub.org/public/igem/BBa_C0060/annotation1755/range1755/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_C0060/annotation1755/range1755/1
type: Range
persistent identity: range1755
display id: range1755
version: 1
top level: aiiA
direction: direction/forward
start: 784
end: 789
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213987
display id: annotation2213987
version: 1
top level: aiiA
location: https://synbiohub.org/public/igem/BBa_C0060/annotation2213987/range2213987/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_C0060/annotation2213987/range2213987/1
type: Range
persistent identity: range2213987
display id: range2213987
version: 1
top level: aiiA
direction: direction/forward
start: 790
end: 814
orientation: inline

mRFP1
type: ComponentDefinition
persistent identity: BBa E1010
display id: BBa_E1010
version: 1
was generated by: iGEM to SBOL conversion
description: **highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)
created: 2004-07-27T11:00:00Z
modified: 2015-08-31T04:07:26Z
mutable provenance: Campbell et al., PNAS v99 p7877 URL
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: monomeric RFP: Red Fluorescent Protein. Excitation peak: 584 nm Emission peak: 607 nm
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_1_
m user id: 0
owner id: 52
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: TAATAA double stop codon added (DE). Four silent mutations made to remove three EcoRI sites and one PstI site: A28G, A76G, A349G, G337A.
star: true
creator: Drew Endy
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: mrfp1
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_E1010_sequence/1

mrfp1
type: SequenceAnnotation
persistent identity: annotation1014044
display id: annotation1014044
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_E1010/annotation1014044/range1014044/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_E1010/annotation1014044/range1014044/1
type: Range
persistent identity: range1014044
display id: range1014044
version: 1
top level: mRFP1
direction: direction/forward
start: 1
end: 675
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214014
display id: annotation2214014
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_E1010/annotation2214014/range2214014/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_E1010/annotation2214014/range2214014/1
type: Range
persistent identity: range2214014
display id: range2214014
version: 1
top level: mRFP1
direction: direction/forward
start: 682
end: 706
orientation: inline

https://synbiohub.org/public/igem/BBa_J48108_sequence/1
type: Sequence
persistent identity: BBa J48108 sequence
display id: BBa_J48108_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatactagagaaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttatgacaacttgacggctacatcattcactttttcttcacaacc…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I6114_sequence/1
type: Sequence
persistent identity: BBa I6114 sequence
display id: BBa_I6114_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agtttattcttgacatggtcatattacggtgagatactcccacgcgcgtgggtactagagaaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J48107_sequence/1
type: Sequence
persistent identity: BBa J48107 sequence
display id: BBa_J48107_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggatccatggaggatcaggatgctgcatttatcaaacaggctacagaagcaatagtggatgtatcattaaatatagataacatagatcctataataaaagagttattagaaagggtaaggaataggcaaaacaggttacaaaataaaaaaccagcactcataccggcagaaaatggtgttgatataaatagtcaaggcggtaacataaaggttaaaaaggaaaacgcattaccaaaaccaccgaagtccagcaaaagcaaaccccaagatcgtagaaatagtactggtgaaaaaagatttaaatgtgcgaaatgttcgttggaattttcaagatcatcagatttgagaaggcacgaaaagacacacttcgccatattgcctaacatttgtcctcaatgtggcaaaggttttgcaaggaaagatgcattgaaaagacattatgatacactgacatgtaggagaaacaggactaaattactaactgcgggtggtgagggtatcaatgaattactgaaaaaagtcaagcaatccaacatcgttcatcgtcaagataacaaccacaatggtagcagtaatggctgagatatc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J48109_sequence/1
type: Sequence
persistent identity: BBa J48109 sequence
display id: BBa_J48109_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatactagagaaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttatgacaacttgacggctacatcattcactttttcttcacaacc…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J48110_sequence/1
type: Sequence
persistent identity: BBa J48110 sequence
display id: BBa_J48110_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tctagattatgaccatcgaggcgatcgcgagatgcatggatgaggtagccatgcattttaatgagcgcataaaaatactggtagctccgctagcgctctacgtacctactccatcggtacgtaaaattactcgcgtattttactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J48111_sequence/1
type: Sequence
persistent identity: BBa J48111 sequence
display id: BBa_J48111_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taatcagtatgacgaatacttaaaatcgtcatacttattttactagagaaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J48112_sequence/1
type: Sequence
persistent identity: BBa J48112 sequence
display id: BBa_J48112_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgcccagacagatagctttctacggtaaaggcggaatcggcaagtcgacaacgtcgcagaacaccctcgcggcgctggtcgagatgggtcagaaaatcctgatcgtcggctgcgatcccaaggcggactcgacccgcctgatcctcaataccaagctgcaggacaccgtgctggcgctcgcagccgaggcgggctcggtcgaagacctcgagctcgaagacgtgatgaagatcggctacaagggcatcaagtgcaccgaagccggtggcccggagccgggcgtcggctgcgccggccgcggcgttatcaccgcgatcaacttcctcgaagagaacggcgcctatgaggacgtcgactacgtctcctacgacgtgctcggcgacgtggtgtgtggcggcttcgcgatgccgatccgtgagaacaaggcgcaggaaatctacatcgtcatgtcgggcgagatgatggcgctgtacgccgccaacaacatctccaagggcattctgaagtacgcctcgtcgggcggtgtccgcctcggtggcctgatctgcaacgagcgccagaccgaccgcgagctcgacctcgccgaagcgctggctgccaagctgaactcgaagctgatccacttcgtgccgcgcgacaacatcgtgcagcatgccgagcttcgccgcgagactgtgatccagtacgcccccgactcgcagcaggcaaaggaataccgtgcgctggccagcaagattcacgcgaattccggcaacggcaccatcccgacgccgatcaccatggaagaactggaagagatgctgctcgacttcggcatcatgaagaccgaggaacagcagctcgccgagctggccgccaaggaagccgccaaggcggcggcggtcgcctgatactagagaaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtgaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacatcagccacaacgtctatatcaccgccgacaagcagaagaacggcatcaaggccaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagaggcctgctgcaaacgacgaaaactacgctttagtagcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R2001_sequence/1
type: Sequence
persistent identity: BBa R2001 sequence
display id: BBa_R2001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agtttattcttgacatggtcatattacggtgagatactcccacgcgcgtggg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I13207_sequence/1
type: Sequence
persistent identity: BBa I13207 sequence
display id: BBa_I13207_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatactagagaaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttatgacaacttgacggctacatcattcactttttcttcacaacc…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J48103_sequence/1
type: Sequence
persistent identity: BBa J48103 sequence
display id: BBa_J48103_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tctagattatgaccatcgaggcgatcgcgagatgcatggatgaggtagccatgcattttaatgagcgcataaaaatactggtagctccgctagcgctctacgtacctactccatcggtacgtaaaattactcgcgtattt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J48104_sequence/1
type: Sequence
persistent identity: BBa J48104 sequence
display id: BBa_J48104_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taatcagtatgacgaatacttaaaatcgtcatacttattt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J48106_sequence/1
type: Sequence
persistent identity: BBa J48106 sequence
display id: BBa_J48106_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgcccagacagatagctttctacggtaaaggcggaatcggcaagtcgacaacgtcgcagaacaccctcgcggcgctggtcgagatgggtcagaaaatcctgatcgtcggctgcgatcccaaggcggactcgacccgcctgatcctcaataccaagctgcaggacaccgtgctggcgctcgcagccgaggcgggctcggtcgaagacctcgagctcgaagacgtgatgaagatcggctacaagggcatcaagtgcaccgaagccggtggcccggagccgggcgtcggctgcgccggccgcggcgttatcaccgcgatcaacttcctcgaagagaacggcgcctatgaggacgtcgactacgtctcctacgacgtgctcggcgacgtggtgtgtggcggcttcgcgatgccgatccgtgagaacaaggcgcaggaaatctacatcgtcatgtcgggcgagatgatggcgctgtacgccgccaacaacatctccaagggcattctgaagtacgcctcgtcgggcggtgtccgcctcggtggcctgatctgcaacgagcgccagaccgaccgcgagctcgacctcgccgaagcgctggctgccaagctgaactcgaagctgatccacttcgtgccgcgcgacaacatcgtgcagcatgccgagcttcgccgcgagactgtgatccagtacgcccccgactcgcagcaggcaaaggaataccgtgcgctggccagcaagattcacgcgaattccggcaacggcaccatcccgacgccgatcaccatggaagaactggaagagatgctgctcgacttcggcatcatgaagaccgaggaacagcagctcgccgagctggccgccaaggaagccgccaaggcggcggcggtcgcctga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0030_sequence/1
type: Sequence
persistent identity: BBa E0030 sequence
display id: BBa_E0030_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0061_sequence/1
type: Sequence
persistent identity: BBa C0061 sequence
display id: BBa_C0061_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I13507_sequence/1
type: Sequence
persistent identity: BBa I13507 sequence
display id: BBa_I13507_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0022_sequence/1
type: Sequence
persistent identity: BBa E0022 sequence
display id: BBa_E0022_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtgaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacatcagccacaacgtctatatcaccgccgacaagcagaagaacggcatcaaggccaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagaggcctgctgcaaacgacgaaaactacgctttagtagcttaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0040_sequence/1
type: Sequence
persistent identity: BBa R0040 sequence
display id: BBa_R0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0062_sequence/1
type: Sequence
persistent identity: BBa C0062 sequence
display id: BBa_C0062_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0062_sequence/1
type: Sequence
persistent identity: BBa R0062 sequence
display id: BBa_R0062_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I0500_sequence/1
type: Sequence
persistent identity: BBa I0500 sequence
display id: BBa_I0500_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccatacccgtttttttgggctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E1010_sequence/1
type: Sequence
persistent identity: BBa E1010 sequence
display id: BBa_E1010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0060_sequence/1
type: Sequence
persistent identity: BBa C0060 sequence
display id: BBa_C0060_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgacagtaaagaagctttatttcgtcccagcaggtcgttgtatgttggatcattcgtctgttaatagtacattaacaccaggagaattattagacttaccggtttggtgttatcttttggagactgaagaaggacctattttagtagatacaggtatgccagaaagtgcagttaataatgaaggtctttttaacggtacatttgtcgaagggcaggttttaccgaaaatgactgaagaagatagaatcgtgaatattttaaaacgggttggttatgagccggaagaccttctttatattattagttctcacttgcattttgatcatgcaggaggaaatggcgcttttataaatacaccaatcattgtacagcgtgctgaatatgaggcggcgcagcatagcgaagaatatttgaaagaatgtatattgccgaatttaaactacaaaatcattgaaggtgattatgaagtcgtaccaggagttcaattattgcatacaccaggccatactccagggcatcaatcgctattaattgagacagaaaaatccggtcctgtattattaacgattgatgcatcgtatacgaaagagaattttgaaaatgaagtgccatttgcgggatttgattcagaattagctttatcttcaattaaacgtttaaaagaagtggtgatgaaagagaagccgattgttttctttggacatgatatagagcaggaaaggggatgtaaagtgttccctgaatatatagctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z