BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation4184 1 stem_loop range4184 1 12 55 annotation7018 1 BBa_B0010 range7018 1 1 80 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_E0022 1 ECFP enhanced cyan fluorescent protein derived from A. victoria GFP 2003-01-31T12:00:00Z 2015-08-31T04:07:25Z Modified from <bb_part>BBa_E0021</bb_part>. Released HQ 2013 Cyan fluorescent protein (ECFP) reporter coding sequence without the Ribosome Binding Site. Modified with an LVA tail for rapid degradation of the protein and faster fall time for the emission. </P> false false _1_ 0 24 7 In stock false <P> <P>BBa_E0022 cyan fluorescent protein is based on BioBrick part BBa_E0021. It has been modified to include a rapid degradation LVA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA. <P> true June Rhee, Connie Tao, Ty Thomson, Louis Waldman annotation2153 1 SsrA range2153 1 719 756 annotation2150 1 CFP (LVA) range2150 1 1 762 annotation2154 1 2 range2154 1 757 762 annotation7040 1 BBa_E0022 range7040 1 1 762 annotation2155 1 A range2155 1 69 69 BBa_J48112 1 BBa_J48112 vnfH: vanadium promoter 2006-11-03T12:00:00Z 2015-08-31T04:08:51Z PVAMU Vanadium promoter false false _73_ 0 969 73 Not in stock false Cut sites for EcoRI and PstI false Laina Magaya component1909784 1 BBa_B0012 component1909774 1 BBa_J48106 component1909782 1 BBa_B0010 component1909778 1 BBa_E0022 component1909776 1 BBa_B0034 annotation1909776 1 BBa_B0034 range1909776 1 900 911 annotation1909782 1 BBa_B0010 range1909782 1 1688 1767 annotation1909784 1 BBa_B0012 range1909784 1 1776 1816 annotation1909774 1 BBa_J48106 range1909774 1 1 891 annotation1909778 1 BBa_E0022 range1909778 1 918 1679 BBa_J48106 1 BBa_J48106 vnfH 2006-10-30T12:00:00Z 2015-08-31T04:08:51Z nitrogenase iron protein, vnfH Rhodopseudomonas palustris CGA009 Vanadium promoter false false _73_ 0 969 73 Not in stock false cut with Xba I and Pst I false Laina Magaya BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1690 1 polya range1690 1 28 41 annotation1686 1 T7 TE range1686 1 8 27 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1687 1 stop range1687 1 34 34 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_J48112_sequence 1 atgcccagacagatagctttctacggtaaaggcggaatcggcaagtcgacaacgtcgcagaacaccctcgcggcgctggtcgagatgggtcagaaaatcctgatcgtcggctgcgatcccaaggcggactcgacccgcctgatcctcaataccaagctgcaggacaccgtgctggcgctcgcagccgaggcgggctcggtcgaagacctcgagctcgaagacgtgatgaagatcggctacaagggcatcaagtgcaccgaagccggtggcccggagccgggcgtcggctgcgccggccgcggcgttatcaccgcgatcaacttcctcgaagagaacggcgcctatgaggacgtcgactacgtctcctacgacgtgctcggcgacgtggtgtgtggcggcttcgcgatgccgatccgtgagaacaaggcgcaggaaatctacatcgtcatgtcgggcgagatgatggcgctgtacgccgccaacaacatctccaagggcattctgaagtacgcctcgtcgggcggtgtccgcctcggtggcctgatctgcaacgagcgccagaccgaccgcgagctcgacctcgccgaagcgctggctgccaagctgaactcgaagctgatccacttcgtgccgcgcgacaacatcgtgcagcatgccgagcttcgccgcgagactgtgatccagtacgcccccgactcgcagcaggcaaaggaataccgtgcgctggccagcaagattcacgcgaattccggcaacggcaccatcccgacgccgatcaccatggaagaactggaagagatgctgctcgacttcggcatcatgaagaccgaggaacagcagctcgccgagctggccgccaaggaagccgccaaggcggcggcggtcgcctgatactagagaaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtgaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacatcagccacaacgtctatatcaccgccgacaagcagaagaacggcatcaaggccaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagaggcctgctgcaaacgacgaaaactacgctttagtagcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0034_sequence 1 aaagaggagaaa BBa_E0022_sequence 1 atggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtgaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacatcagccacaacgtctatatcaccgccgacaagcagaagaacggcatcaaggccaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagaggcctgctgcaaacgacgaaaactacgctttagtagcttaataa BBa_J48106_sequence 1 atgcccagacagatagctttctacggtaaaggcggaatcggcaagtcgacaacgtcgcagaacaccctcgcggcgctggtcgagatgggtcagaaaatcctgatcgtcggctgcgatcccaaggcggactcgacccgcctgatcctcaataccaagctgcaggacaccgtgctggcgctcgcagccgaggcgggctcggtcgaagacctcgagctcgaagacgtgatgaagatcggctacaagggcatcaagtgcaccgaagccggtggcccggagccgggcgtcggctgcgccggccgcggcgttatcaccgcgatcaacttcctcgaagagaacggcgcctatgaggacgtcgactacgtctcctacgacgtgctcggcgacgtggtgtgtggcggcttcgcgatgccgatccgtgagaacaaggcgcaggaaatctacatcgtcatgtcgggcgagatgatggcgctgtacgccgccaacaacatctccaagggcattctgaagtacgcctcgtcgggcggtgtccgcctcggtggcctgatctgcaacgagcgccagaccgaccgcgagctcgacctcgccgaagcgctggctgccaagctgaactcgaagctgatccacttcgtgccgcgcgacaacatcgtgcagcatgccgagcttcgccgcgagactgtgatccagtacgcccccgactcgcagcaggcaaaggaataccgtgcgctggccagcaagattcacgcgaattccggcaacggcaccatcccgacgccgatcaccatggaagaactggaagagatgctgctcgacttcggcatcatgaagaccgaggaacagcagctcgccgagctggccgccaaggaagccgccaaggcggcggcggtcgcctga BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z