BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1690
1
polya
range1690
1
28
41
annotation7020
1
BBa_B0012
range7020
1
1
41
annotation1686
1
T7 TE
range1686
1
8
27
annotation1687
1
stop
range1687
1
34
34
BBa_J49001
1
BBa_J49001
Testosterone dependent promoter for species Bicyclus Bicyclus
2006-06-14T11:00:00Z
2015-08-31T04:08:51Z
Henry's Mustache
This part will be used to turn young bicycles into mature motorcycles ready to take on the world. VROOM!
false
false
_74_
0
709
74
Not in stock
false
Do not mix with alcohol.
false
John Mangual
annotation1880742
1
binding site
range1880742
1
1
12
BBa_J49015
1
BBa_J49015
Motorcycle adolescence module
2006-06-14T11:00:00Z
2015-08-31T03:54:03Z
BBa_J49003 and BBa_J49001
This is the composite output module comprised of the testosterone promoter and the motorcycle gene.
false
true
_74_
0
704
74
Not in stock
false
Again, just the fact that Bicyclus bicyclus is not made from testosterone. Oh well.
false
Ritu Kamal
component1880767
1
BBa_J49001
component1880772
1
BBa_J49003
component1880769
1
BBa_B0030
component1880773
1
BBa_B0012
annotation1880773
1
BBa_B0012
range1880773
1
166
206
annotation1880772
1
BBa_J49003
range1880772
1
121
157
annotation1880767
1
BBa_J49001
range1880767
1
1
89
annotation1880769
1
BBa_B0030
range1880769
1
98
112
BBa_B0030
1
BBa_B0030
RBS.1 (strong) -- modified from R. Weiss
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_44_46_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation1702
1
RBS
range1702
1
8
12
annotation1701
1
RBS-1\Strong
range1701
1
1
15
annotation7025
1
BBa_B0030
range7025
1
1
15
BBa_J49003
1
BBa_J49003
Gene to make a motorcycle
2006-06-14T11:00:00Z
2015-08-31T04:08:51Z
Draws inspiration from Henry's Moustache
This part makes a motorcycle under the influence of the bicycle dependent promoter.
false
false
_74_
0
704
74
Not in stock
false
the fact that testosterone does not make a bicycle
false
Ritu Kamal
annotation1880743
1
MtrC
range1880743
1
1
35
BBa_J49003_sequence
1
aaaaaattggaatatatatatatatgcgcgcagatgc
BBa_J49015_sequence
1
aggactagataggacacagattagacgagatacccagatacgatagacgatagggatagaccatagactagaacgatactagactacgatactagagattaaagaggagaaatactagagaaaaaattggaatatatatatatatgcgcgcagatgctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_B0030_sequence
1
attaaagaggagaaa
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_J49001_sequence
1
aggactagataggacacagattagacgagatacccagatacgatagacgatagggatagaccatagactagaacgatactagactacga
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z